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The map label for this gene is aroF [H]

Identifier: 33864512

GI number: 33864512

Start: 2376113

End: 2377195

Strand: Reverse

Name: aroF [H]

Synonym: PMT2248

Alternate gene names: 33864512

Gene position: 2377195-2376113 (Counterclockwise)

Preceding gene: 33864516

Following gene: 33864507

Centisome position: 98.6

GC content: 52.35

Gene sequence:

>1083_bases
ATGAATTCTGGCGAAATGACCACCACTTCCGACTTGCATGTGGTGGATACGCGGCCCTTGGTGTCGCCGCTCTTGCTTCA
TCAGGAGCTGCCCCTTGATCTAGTGGCTCTCAAAACCGTTGCAGATACTCGTAAGCGCATCCAGGCAATTCTGCGTGGTG
AGGATCCCCGCTTGCTGGTGATTGTTGGTCCCTGCTCGGTTCATGACATTGCCTCTGCAAGGGACTATGCCCGTCAGTTG
GAGCCATTGCGACAGCGATATGCCGCCCAGTTGGAGGTGGTTTTGCGGGTCTATTTCGAAAAACCTCGCACCACAGTTGG
CTGGAAAGGTCTCATCAATGATCCCCATCTCGATGGTTCCTATGACATCAACACTGGCTTGAGGCGTGCGCGGTCGTTGT
TGCTCGACCTTGCTCGCGAGGGTATGCCGACGGCCACTGAATTGCTGGATCCGGTTGTGCCTCAATACATCGCTGATTTG
ATCAGTTGGACGGCGATTGGAGCCAGAACCACTGAGAGTCAGACCCATCGGGAGATGGCTTCTGGATTGTCAATGCCCGT
CGGTTACAAAAACGGTACCGATGGCAGTGCCAAGATTGCGATCCATGCGATGCAGGCAGCATCTAGACCGCATCATTTTC
TTGGGATCAATCGGCAGGGTCAGGCTTCGATTGTGCATACCACTGGAAACCCTGATGGCCATCTCGTGTTGCGGGGAGGT
AATGGCTGTACCAATTACCATCCCGAAGCGGTGGAAGAGGTTGCAAAAGAATTAGTGAAGGCTGGCTTGGTTGATCGGTT
GATGGTGGATTGCAGCCATGACAATTCGAAGAAAGATTTTCGGCGACAGTCAGAGGTGCTTCAGGCTGTTGCTACTCAGG
TACGCCAAGGATCAACCCATCTCATGGGTGTGATGTTGGAAAGTCATCTTGTCGAGGGCAATCAGAAGTTGCCTGAAGAC
CTCTCTACTCTTGTCTATGGTCAAAGCATTACGGATGCTTGTATCGATATAGAGACAACGGCAACTCTCCTTGAGGATTT
GGCGGCTGCAGTGGCTTCAGTGACGTTGTCACCAATAACTTGA

Upstream 100 bases:

>100_bases
TTCTATTGTTATTTGCCGTCGATCCATCCTGGAAGCAGATCGATATTGAAATGCAATTACCGGATGCTGCCACCTTGCTG
GTTTCCTAGCCTGGGTTGTC

Downstream 100 bases:

>100_bases
TCAGCCTTTAGATTGCTGTCATCCATGACCAGGCGACTGCAACTCCTATCGGCACAGTGAGGTTGTCGATACCCCAGCGA
CTTAGTTGCTCTAGTCCAAC

Product: phospho-2-dehydro-3-deoxyheptonate aldolase

Products: NA

Alternate protein names: 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase; DAHP synthase; Phospho-2-keto-3-deoxyheptonate aldolase [H]

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MNSGEMTTTSDLHVVDTRPLVSPLLLHQELPLDLVALKTVADTRKRIQAILRGEDPRLLVIVGPCSVHDIASARDYARQL
EPLRQRYAAQLEVVLRVYFEKPRTTVGWKGLINDPHLDGSYDINTGLRRARSLLLDLAREGMPTATELLDPVVPQYIADL
ISWTAIGARTTESQTHREMASGLSMPVGYKNGTDGSAKIAIHAMQAASRPHHFLGINRQGQASIVHTTGNPDGHLVLRGG
NGCTNYHPEAVEEVAKELVKAGLVDRLMVDCSHDNSKKDFRRQSEVLQAVATQVRQGSTHLMGVMLESHLVEGNQKLPED
LSTLVYGQSITDACIDIETTATLLEDLAAAVASVTLSPIT

Sequences:

>Translated_360_residues
MNSGEMTTTSDLHVVDTRPLVSPLLLHQELPLDLVALKTVADTRKRIQAILRGEDPRLLVIVGPCSVHDIASARDYARQL
EPLRQRYAAQLEVVLRVYFEKPRTTVGWKGLINDPHLDGSYDINTGLRRARSLLLDLAREGMPTATELLDPVVPQYIADL
ISWTAIGARTTESQTHREMASGLSMPVGYKNGTDGSAKIAIHAMQAASRPHHFLGINRQGQASIVHTTGNPDGHLVLRGG
NGCTNYHPEAVEEVAKELVKAGLVDRLMVDCSHDNSKKDFRRQSEVLQAVATQVRQGSTHLMGVMLESHLVEGNQKLPED
LSTLVYGQSITDACIDIETTATLLEDLAAAVASVTLSPIT
>Mature_360_residues
MNSGEMTTTSDLHVVDTRPLVSPLLLHQELPLDLVALKTVADTRKRIQAILRGEDPRLLVIVGPCSVHDIASARDYARQL
EPLRQRYAAQLEVVLRVYFEKPRTTVGWKGLINDPHLDGSYDINTGLRRARSLLLDLAREGMPTATELLDPVVPQYIADL
ISWTAIGARTTESQTHREMASGLSMPVGYKNGTDGSAKIAIHAMQAASRPHHFLGINRQGQASIVHTTGNPDGHLVLRGG
NGCTNYHPEAVEEVAKELVKAGLVDRLMVDCSHDNSKKDFRRQSEVLQAVATQVRQGSTHLMGVMLESHLVEGNQKLPED
LSTLVYGQSITDACIDIETTATLLEDLAAAVASVTLSPIT

Specific function: Stereospecific condensation of phosphoenolpyruvate (PEP) and D-erythrose-4-phosphate (E4P) giving rise to 3-deoxy-D- arabino-heptulosonate-7-phosphate (DAHP) [H]

COG id: COG0722

COG function: function code E; 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I DAHP synthase family [H]

Homologues:

Organism=Escherichia coli, GI1788953, Length=338, Percent_Identity=55.0295857988166, Blast_Score=397, Evalue=1e-112,
Organism=Escherichia coli, GI1786969, Length=345, Percent_Identity=54.4927536231884, Blast_Score=379, Evalue=1e-106,
Organism=Escherichia coli, GI1787996, Length=346, Percent_Identity=52.0231213872832, Blast_Score=354, Evalue=4e-99,
Organism=Saccharomyces cerevisiae, GI6319726, Length=342, Percent_Identity=49.7076023391813, Blast_Score=344, Evalue=1e-95,
Organism=Saccharomyces cerevisiae, GI6320240, Length=355, Percent_Identity=45.9154929577465, Blast_Score=337, Evalue=1e-93,

Paralogues:

None

Copy number: 2,200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006218
- InterPro:   IPR006219 [H]

Pfam domain/function: PF00793 DAHP_synth_1 [H]

EC number: =2.5.1.54 [H]

Molecular weight: Translated: 39292; Mature: 39292

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSGEMTTTSDLHVVDTRPLVSPLLLHQELPLDLVALKTVADTRKRIQAILRGEDPRLLV
CCCCCCCCCCCEEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
IVGPCSVHDIASARDYARQLEPLRQRYAAQLEVVLRVYFEKPRTTVGWKGLINDPHLDGS
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
YDINTGLRRARSLLLDLAREGMPTATELLDPVVPQYIADLISWTAIGARTTESQTHREMA
CCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
SGLSMPVGYKNGTDGSAKIAIHAMQAASRPHHFLGINRQGQASIVHTTGNPDGHLVLRGG
CCCCCCCCCCCCCCCCCEEEEEEHHHCCCCCCEEECCCCCCEEEEEECCCCCCEEEEECC
NGCTNYHPEAVEEVAKELVKAGLVDRLMVDCSHDNSKKDFRRQSEVLQAVATQVRQGSTH
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHH
LMGVMLESHLVEGNQKLPEDLSTLVYGQSITDACIDIETTATLLEDLAAAVASVTLSPIT
HHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNSGEMTTTSDLHVVDTRPLVSPLLLHQELPLDLVALKTVADTRKRIQAILRGEDPRLLV
CCCCCCCCCCCEEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
IVGPCSVHDIASARDYARQLEPLRQRYAAQLEVVLRVYFEKPRTTVGWKGLINDPHLDGS
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
YDINTGLRRARSLLLDLAREGMPTATELLDPVVPQYIADLISWTAIGARTTESQTHREMA
CCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
SGLSMPVGYKNGTDGSAKIAIHAMQAASRPHHFLGINRQGQASIVHTTGNPDGHLVLRGG
CCCCCCCCCCCCCCCCCEEEEEEHHHCCCCCCEEECCCCCCEEEEEECCCCCCEEEEECC
NGCTNYHPEAVEEVAKELVKAGLVDRLMVDCSHDNSKKDFRRQSEVLQAVATQVRQGSTH
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHH
LMGVMLESHLVEGNQKLPEDLSTLVYGQSITDACIDIETTATLLEDLAAAVASVTLSPIT
HHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6146618; 6396419; 9205837; 9278503; 1977738; 6104668; 2857723; 9056491 [H]