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The map label for this gene is rpoD4 [H]

Identifier: 33864510

GI number: 33864510

Start: 2374409

End: 2375443

Strand: Direct

Name: rpoD4 [H]

Synonym: PMT2246

Alternate gene names: 33864510

Gene position: 2374409-2375443 (Clockwise)

Preceding gene: 33864509

Following gene: 33864511

Centisome position: 98.49

GC content: 53.72

Gene sequence:

>1035_bases
ATGGGGATCCCTCTGGAATCTGCGAAGGGTGCTTCACTCACGCCTTCGTCAGAAGTTGTATTACCGTCTACATCTAAGCA
ACGTTCAGAAACAGCGAATCGAGCTGGCCGTAACGGGCAAGCATCCCGTAATCAAAATCGCCAAGGTGGACGTTTGGGTA
CTGATGCGATCGGGTTCTACCTGAGCAGCATTGGACGCGTGCCTTTGCTGACCGCAGCTGAGGAAATTGAGCTTGCCCAC
CATGTGCAAGCGATGAAGGAATTGCTGGAGTTACCAGAGCAAGACCAAACGCCACGACAACGCCACAAAATTCGCATGGG
CAAACGCGCCCGTGACCGCATGATGTCAGCCAACCTCCGGCTCGTGGTAAGCGTTGCCAAAAAATATCAAAATCAGGGCC
TTGAACTCCTTGACCTAGTCCAAGAAGGAGCCATTGGTCTCGAACGTGCTGTCGACAAGTTCGATCCAGCCATGGGTTAT
AAGTTCTCCACCTACGCCTATTGGTGGATTCGTCAAGGGATGACCAGGGCCATCGACAACAGTGCCCGCACCATCCGTCT
ACCCATCCACATCAGCGAAAAACTCTCCAAGATGCGACGCATCTCAAGAGAGCTTTCCCATCGCTTCGGCCGTCAACCAA
ATCGATTGGAGTTAGCCCATGCCATGGGTATTCAACCCCAAGACCTTGAGGATCTCATCGCTCAAAGCGCTCCTTGCGCA
TCCCTCGATGCCCATGCCCGCGGAGAAGAAGACCGCAGCACCCTAGGTGAACTGATCCCCGACCCCGATGGGGCCGAACC
AATGGAAGGCCTAGATCGCAGCATCCAAAAGGAACACCTAGGAGGTTGGCTATCTCAGCTCAATGAACGTGAACAGAAAA
TCCTGCGCTTGCGCTTCGGTCTAGATGGTGAAGAACCACTGACCCTCGCTGAAATCGGGCGGCAAATCAGCGTCTCACGA
GAACGCGTACGACAGTTGGAGGCCAAAGCCATTCTCAAGCTACGGATGATGACCAACCATCAACAAGCTGCATGA

Upstream 100 bases:

>100_bases
GATCGCTCTCATGGGCTAACAAAAACTTTTTGCTTCAACCAAGTTTCTAAGCTCTATGAGTGCTACATTCCGCGCAAATC
TAATTTAGGAGGCCAGGGTG

Downstream 100 bases:

>100_bases
AATAAGTCTTGGGGTATATCAACTCTCTGGTTGCCATTGCATGCTGGATAGCTTTTGTGCTTTCAGCTGCTGTGGTTTGC
AGGGTCCGCTGGCCTAACCA

Product: type II alternative sigma-70 family RNA polymerase sigma factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 343

Protein sequence:

>344_residues
MGIPLESAKGASLTPSSEVVLPSTSKQRSETANRAGRNGQASRNQNRQGGRLGTDAIGFYLSSIGRVPLLTAAEEIELAH
HVQAMKELLELPEQDQTPRQRHKIRMGKRARDRMMSANLRLVVSVAKKYQNQGLELLDLVQEGAIGLERAVDKFDPAMGY
KFSTYAYWWIRQGMTRAIDNSARTIRLPIHISEKLSKMRRISRELSHRFGRQPNRLELAHAMGIQPQDLEDLIAQSAPCA
SLDAHARGEEDRSTLGELIPDPDGAEPMEGLDRSIQKEHLGGWLSQLNEREQKILRLRFGLDGEEPLTLAEIGRQISVSR
ERVRQLEAKAILKLRMMTNHQQAA

Sequences:

>Translated_344_residues
MGIPLESAKGASLTPSSEVVLPSTSKQRSETANRAGRNGQASRNQNRQGGRLGTDAIGFYLSSIGRVPLLTAAEEIELAH
HVQAMKELLELPEQDQTPRQRHKIRMGKRARDRMMSANLRLVVSVAKKYQNQGLELLDLVQEGAIGLERAVDKFDPAMGY
KFSTYAYWWIRQGMTRAIDNSARTIRLPIHISEKLSKMRRISRELSHRFGRQPNRLELAHAMGIQPQDLEDLIAQSAPCA
SLDAHARGEEDRSTLGELIPDPDGAEPMEGLDRSIQKEHLGGWLSQLNEREQKILRLRFGLDGEEPLTLAEIGRQISVSR
ERVRQLEAKAILKLRMMTNHQQAA
>Mature_343_residues
GIPLESAKGASLTPSSEVVLPSTSKQRSETANRAGRNGQASRNQNRQGGRLGTDAIGFYLSSIGRVPLLTAAEEIELAHH
VQAMKELLELPEQDQTPRQRHKIRMGKRARDRMMSANLRLVVSVAKKYQNQGLELLDLVQEGAIGLERAVDKFDPAMGYK
FSTYAYWWIRQGMTRAIDNSARTIRLPIHISEKLSKMRRISRELSHRFGRQPNRLELAHAMGIQPQDLEDLIAQSAPCAS
LDAHARGEEDRSTLGELIPDPDGAEPMEGLDRSIQKEHLGGWLSQLNEREQKILRLRFGLDGEEPLTLAEIGRQISVSRE
RVRQLEAKAILKLRMMTNHQQAA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=229, Percent_Identity=45.8515283842795, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI1789098, Length=287, Percent_Identity=39.0243902439024, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI1789871, Length=284, Percent_Identity=26.4084507042254, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1788231, Length=210, Percent_Identity=30, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR017848
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 38630; Mature: 38499

Theoretical pI: Translated: 10.09; Mature: 10.09

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIPLESAKGASLTPSSEVVLPSTSKQRSETANRAGRNGQASRNQNRQGGRLGTDAIGFY
CCCCCCCCCCCCCCCCCCEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHH
LSSIGRVPLLTAAEEIELAHHVQAMKELLELPEQDQTPRQRHKIRMGKRARDRMMSANLR
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCHH
LVVSVAKKYQNQGLELLDLVQEGAIGLERAVDKFDPAMGYKFSTYAYWWIRQGMTRAIDN
HHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHCHHCCCCHHHHHHHHHHHHHHHHHCC
SARTIRLPIHISEKLSKMRRISRELSHRFGRQPNRLELAHAMGIQPQDLEDLIAQSAPCA
CCCEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHCCCCCC
SLDAHARGEEDRSTLGELIPDPDGAEPMEGLDRSIQKEHLGGWLSQLNEREQKILRLRFG
CCCHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
LDGEEPLTLAEIGRQISVSRERVRQLEAKAILKLRMMTNHQQAA
CCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
GIPLESAKGASLTPSSEVVLPSTSKQRSETANRAGRNGQASRNQNRQGGRLGTDAIGFY
CCCCCCCCCCCCCCCCCEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHH
LSSIGRVPLLTAAEEIELAHHVQAMKELLELPEQDQTPRQRHKIRMGKRARDRMMSANLR
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCHH
LVVSVAKKYQNQGLELLDLVQEGAIGLERAVDKFDPAMGYKFSTYAYWWIRQGMTRAIDN
HHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHCHHCCCCHHHHHHHHHHHHHHHHHCC
SARTIRLPIHISEKLSKMRRISRELSHRFGRQPNRLELAHAMGIQPQDLEDLIAQSAPCA
CCCEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHCCCCCC
SLDAHARGEEDRSTLGELIPDPDGAEPMEGLDRSIQKEHLGGWLSQLNEREQKILRLRFG
CCCHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
LDGEEPLTLAEIGRQISVSRERVRQLEAKAILKLRMMTNHQQAA
CCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA