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The map label for this gene is tetA [H]

Identifier: 33864508

GI number: 33864508

Start: 2370712

End: 2372019

Strand: Direct

Name: tetA [H]

Synonym: PMT2244

Alternate gene names: 33864508

Gene position: 2370712-2372019 (Clockwise)

Preceding gene: 33864506

Following gene: 33864509

Centisome position: 98.33

GC content: 53.29

Gene sequence:

>1308_bases
ATGGCGTCAAGTCTCTATGGGATCGTGACATTCATCCTGACTCATTCCCAATTGCGCCGACCACAGATTCCCATCTTTCT
CTGTGCCTTTGTCACTCTGTTGAACGATCGCCTAGGTGAAACCCTCTTACTGCCATTACTTCCATACCTCCCAGGACGCT
TCACAGACAGCGGCACAATCCTGGGGCTACTTGGAGGTACTTATGCATTGGCTCAATTCGTCGTGGCTCCCCTAATTGGA
GCTCTCAGTGATCGTTTTGGCCGCAAACCAATTTTAACCGCTTGCGTTGCTGGCTCAGTAGTAGGCCTTGGCTTATTCGC
TATCACAGTATGGATTGACTGGAACATACTTCCAGCCGCTTGGATCGGCATTGTTCCCCTGATCCTTCTCTTCTCAGCAA
GAATCATCGATGGCGTTAGCGGTGGAACAGCAAGTAGCGCCACAGCAGTGCTTGCCGACATCTCAACACCTGAGAGCCGA
GCAAAGGCATTCGGCCTGATTGGCGTTGCATTCGGCCTCGGGTTCATCTTGGGCCCTTATATCGGTGGCCGCTTAGCAGA
GATCAATATTGCTCTACCCGGGATAGCCGCCACAGCCTTCGCCGTCGCGAACCTGCTTCTTGTGATCTATATCCTTCCCG
AAACACACCCGCCAGCAGCTCGCAATTCCCTACCAAGCAAAAGACAACTCAACCCGATCACCCAGCTAGCACAGATCTTT
GCCAATCCATTAGTAAGCCGCCTTTGTTTCGCCTTCTTCCTGTTCTTCATGGCATTCAACGGTTTCACAGCTGTGCTGGT
GCTTTACCTGAAGCAAGCCTTTTCATGGACAGTCGGTTTAGCGGGCCTGACCTTTGCAGTTGTAGGCGTGATCGCAATGG
TGGTTCAAGGGCTGCTCATCGGTCCACTGGTTAAATCCTTCGGCGAATGGCGGCTCACCATTGCTGGCGTTGGCTTCGTC
ATTGCAGGCTGTCTGCTATTGCCCATGGCCAGTCAGCAGAATTCGATTTCTGTTGTATTCACTGCCGTATCGGTACTAGC
CCTTGGCACAGGTCTAGTAGTGCCATGCTTGAGAGCCCTCGTCTCGAGACGCCTCGACAACGCAGGCCAAGGGGCAGTAC
TCGGTAGCCTTCAAGGCCTGCAGAGTCTAGGGACCTTCCTTGGGGCAGCGGCTGCAGGATTCGCCTACGACCAAATAGGC
CCTCGCAGTCCCTTCTGGCTGGCCATCCTCGTACTAACGGGAGTTGTTGCCCTTGTTGCAGGAGGCCCACCTGCAAGCAC
AAGTAACACAACAATCAAACAATCATGA

Upstream 100 bases:

>100_bases
ATTCAATCGCATCGGCATAAATCTCCCTGACAACAAAATGATCAGTTTTTGTAAGGGGTCTTAAGCGGCGACAAGTCACA
AGAGCCTGCCATTTAGATCA

Downstream 100 bases:

>100_bases
TTGCTACTTTGCCTGGATAGTGGATCAACAAACTGGCATTCAATGAGTAATCCAGCAGTAGCTTCGGAGCACTACATCAA
CCGAGAGCTCAGCTGGATCT

Product: major facilitator superfamily multidrug-efflux transporter

Products: NA

Alternate protein names: TetA(C) [H]

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MASSLYGIVTFILTHSQLRRPQIPIFLCAFVTLLNDRLGETLLLPLLPYLPGRFTDSGTILGLLGGTYALAQFVVAPLIG
ALSDRFGRKPILTACVAGSVVGLGLFAITVWIDWNILPAAWIGIVPLILLFSARIIDGVSGGTASSATAVLADISTPESR
AKAFGLIGVAFGLGFILGPYIGGRLAEINIALPGIAATAFAVANLLLVIYILPETHPPAARNSLPSKRQLNPITQLAQIF
ANPLVSRLCFAFFLFFMAFNGFTAVLVLYLKQAFSWTVGLAGLTFAVVGVIAMVVQGLLIGPLVKSFGEWRLTIAGVGFV
IAGCLLLPMASQQNSISVVFTAVSVLALGTGLVVPCLRALVSRRLDNAGQGAVLGSLQGLQSLGTFLGAAAAGFAYDQIG
PRSPFWLAILVLTGVVALVAGGPPASTSNTTIKQS

Sequences:

>Translated_435_residues
MASSLYGIVTFILTHSQLRRPQIPIFLCAFVTLLNDRLGETLLLPLLPYLPGRFTDSGTILGLLGGTYALAQFVVAPLIG
ALSDRFGRKPILTACVAGSVVGLGLFAITVWIDWNILPAAWIGIVPLILLFSARIIDGVSGGTASSATAVLADISTPESR
AKAFGLIGVAFGLGFILGPYIGGRLAEINIALPGIAATAFAVANLLLVIYILPETHPPAARNSLPSKRQLNPITQLAQIF
ANPLVSRLCFAFFLFFMAFNGFTAVLVLYLKQAFSWTVGLAGLTFAVVGVIAMVVQGLLIGPLVKSFGEWRLTIAGVGFV
IAGCLLLPMASQQNSISVVFTAVSVLALGTGLVVPCLRALVSRRLDNAGQGAVLGSLQGLQSLGTFLGAAAAGFAYDQIG
PRSPFWLAILVLTGVVALVAGGPPASTSNTTIKQS
>Mature_434_residues
ASSLYGIVTFILTHSQLRRPQIPIFLCAFVTLLNDRLGETLLLPLLPYLPGRFTDSGTILGLLGGTYALAQFVVAPLIGA
LSDRFGRKPILTACVAGSVVGLGLFAITVWIDWNILPAAWIGIVPLILLFSARIIDGVSGGTASSATAVLADISTPESRA
KAFGLIGVAFGLGFILGPYIGGRLAEINIALPGIAATAFAVANLLLVIYILPETHPPAARNSLPSKRQLNPITQLAQIFA
NPLVSRLCFAFFLFFMAFNGFTAVLVLYLKQAFSWTVGLAGLTFAVVGVIAMVVQGLLIGPLVKSFGEWRLTIAGVGFVI
AGCLLLPMASQQNSISVVFTAVSVLALGTGLVVPCLRALVSRRLDNAGQGAVLGSLQGLQSLGTFLGAAAAGFAYDQIGP
RSPFWLAILVLTGVVALVAGGPPASTSNTTIKQS

Specific function: Resistance to tetracycline by an active tetracycline efflux. This is an energy-dependent process that decreases the accumulation of the antibiotic in whole cells. This protein functions as a metal-tetracycline/H(+) antiporter [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family [H]

Homologues:

Organism=Homo sapiens, GI39753965, Length=340, Percent_Identity=28.2352941176471, Blast_Score=113, Evalue=4e-25,
Organism=Homo sapiens, GI94721311, Length=397, Percent_Identity=26.9521410579345, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI225703104, Length=443, Percent_Identity=23.4762979683973, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI225703102, Length=443, Percent_Identity=23.4762979683973, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI16445357, Length=426, Percent_Identity=26.2910798122066, Blast_Score=71, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71996331, Length=364, Percent_Identity=25.5494505494505, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17551570, Length=344, Percent_Identity=24.4186046511628, Blast_Score=88, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI71985371, Length=368, Percent_Identity=25.8152173913043, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI17738085, Length=433, Percent_Identity=28.4064665127021, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI21357569, Length=361, Percent_Identity=28.5318559556787, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24656463, Length=361, Percent_Identity=28.5318559556787, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24656468, Length=361, Percent_Identity=28.5318559556787, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI161080926, Length=361, Percent_Identity=28.5318559556787, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI161080924, Length=361, Percent_Identity=28.5318559556787, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI221513297, Length=359, Percent_Identity=25.9052924791086, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI24667557, Length=208, Percent_Identity=24.0384615384615, Blast_Score=69, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24667561, Length=196, Percent_Identity=25.5102040816327, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829
- InterPro:   IPR001958 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 45318; Mature: 45187

Theoretical pI: Translated: 10.04; Mature: 10.04

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASSLYGIVTFILTHSQLRRPQIPIFLCAFVTLLNDRLGETLLLPLLPYLPGRFTDSGTI
CCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCE
LGLLGGTYALAQFVVAPLIGALSDRFGRKPILTACVAGSVVGLGLFAITVWIDWNILPAA
EHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
WIGIVPLILLFSARIIDGVSGGTASSATAVLADISTPESRAKAFGLIGVAFGLGFILGPY
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
IGGRLAEINIALPGIAATAFAVANLLLVIYILPETHPPAARNSLPSKRQLNPITQLAQIF
CCCEEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCHHHHHHHHH
ANPLVSRLCFAFFLFFMAFNGFTAVLVLYLKQAFSWTVGLAGLTFAVVGVIAMVVQGLLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GPLVKSFGEWRLTIAGVGFVIAGCLLLPMASQQNSISVVFTAVSVLALGTGLVVPCLRAL
HHHHHHCCCCEEEEHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHH
VSRRLDNAGQGAVLGSLQGLQSLGTFLGAAAAGFAYDQIGPRSPFWLAILVLTGVVALVA
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHHHHHC
GGPPASTSNTTIKQS
CCCCCCCCCCCCCCC
>Mature Secondary Structure 
ASSLYGIVTFILTHSQLRRPQIPIFLCAFVTLLNDRLGETLLLPLLPYLPGRFTDSGTI
CCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCE
LGLLGGTYALAQFVVAPLIGALSDRFGRKPILTACVAGSVVGLGLFAITVWIDWNILPAA
EHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
WIGIVPLILLFSARIIDGVSGGTASSATAVLADISTPESRAKAFGLIGVAFGLGFILGPY
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
IGGRLAEINIALPGIAATAFAVANLLLVIYILPETHPPAARNSLPSKRQLNPITQLAQIF
CCCEEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCHHHHHHHHH
ANPLVSRLCFAFFLFFMAFNGFTAVLVLYLKQAFSWTVGLAGLTFAVVGVIAMVVQGLLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GPLVKSFGEWRLTIAGVGFVIAGCLLLPMASQQNSISVVFTAVSVLALGTGLVVPCLRAL
HHHHHHCCCCEEEEHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHH
VSRRLDNAGQGAVLGSLQGLQSLGTFLGAAAAGFAYDQIGPRSPFWLAILVLTGVVALVA
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHHHHHC
GGPPASTSNTTIKQS
CCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 383387; 6337373; 6307828; 1517220 [H]