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The map label for this gene is nirA [H]

Identifier: 33864503

GI number: 33864503

Start: 2365139

End: 2366767

Strand: Direct

Name: nirA [H]

Synonym: PMT2239

Alternate gene names: 33864503

Gene position: 2365139-2366767 (Clockwise)

Preceding gene: 33864500

Following gene: 33864504

Centisome position: 98.1

GC content: 49.85

Gene sequence:

>1629_bases
ATGGTTATTAGCTCTCCTGCGAAACAATTTCTACAAGGAAAGAAGCTCAACAAAATTGAGCAAAATAAAGCTTTTAAAGA
TGGCTTAGATGTTGGTGATGAACTTGAAAACTTTGCTCAGCTCGGTTGGGAACAGGTCGATAAAACAGACCTCGAACTGC
GCCTGAAATGGTATGGAATGTTCTGGCGACCCAAAACCCCTGGCAAGTTCATGCTACGGCTAAGGATACCCAATGGTGTT
CTCAATAGCCACCAAATAAAAGTAGTGGCCTCAATCGTGGGTCGCTACGGAGAAGACGGCAGTTGCGACATCACAACACG
CCAAAACCTACAGCTTCGTGGTGTTTTACTGAGTGATCTTCCCGAAATCCTCAAACGTCTTGAAGAGGCAGGTCTCAACA
CAATCCAATCAGGTTTTGATAACCCACGCAATGTCACAGGAAATCCACTGGCTGGGATTGACCCTCTTGAAATTGTTGAT
ACTCGCCCATACACAACTGAACTACAAAATTTCCTGACGAATCAAGGAAAGGGAAACCATGAATTCTCCAACCTTCCCCG
CAAATGGAATACGGCAGTTGCAGGGGCCCGCGATAATTTCCTGCTCCATAACGACATCATCTTCCATCCAGTGGAACACG
ATGGGGTGATGGGTTTCGGCGTCTGGCTCGGAGGGATCCTCTCCTCGCAGATGAATGACTATGCGCTGCCTTTAAATGCC
TGGGTTAAACCCAATGAAATCTGTCGGCTCACCGGCACTATTCTAACGTTATGGCGAGATAACGGTGAGCGCAACAATCG
CCCTAAGGGCAGGTTTCGCATGTACCTCGACAGCGTGGGCATCGAAGCGTTTCGCACCATGGTGGTGGAAAAATTTGGAC
CGCTAACACCTGATCCCGGTTCTGTATTTGAAGACAAGCCACGCTCTTTTTACGGAATCCACCCTCAGAAACAAACAGGG
CTTCACTACGCCGGCATGCATGTACCGGTTGGACGATTAAACGCAGAAGATCTTCAGGATCTTGCTAGCGCCAGTCTTAA
CTATGGCAGTGGTGAAATTCGTCTTACAGAAGACCAGAACATCATTTTGGTGGGGCTATCAACTACCAACCTCGACAAAT
TTCAAGCTGATCCACTGCTACAGCGTTTTCCACTTGAGCCCGGTTCCATTGCCGCCGGCACTGTCTCCTGCACAGGAAAT
ACGTACTGCAGCTTCGCCCTTACAAACACCAAAGATCAGGCCATGGCAATGGCACATGAACTGGATGCAGAACTCATCCT
CCCTGAGGAAATAAAAGTCCATTGGACTGGCTGTCCCAACAGCTGTGGCCAGGCCTACATGGGCGCAATCGGCCTCACAG
GAACCAAAGCCCGTGCACCGAACGGGGGCCAAGGCATGGTTGAGGCCTATGACCTCAGCCTTGAAGGCAGGCAAGGGCCG
GATGCCCAAATCGGAACACTGCATCGCAAAGGCATTCCTGGCAGCGAACTGAAAGACGTTCTGCGGGAATTACTGATCGA
ACGCTTCGGTGCTCAACCTCGTAGCCAACCGAAGCAAAACAGCGACCCTTTCTCAAACTTCATGAACTGGTTCAGCAATT
TAGGCGAAAACAGCAGCCCATCCCCTTGA

Upstream 100 bases:

>100_bases
TTCAATAATGGCAGGGCCTAAAAACCATGGCTATCGAATGCGCGAATAAAATTTAAACAACATGATCACTTCTTTTCAAG
TTTAATTTCAACAAAATTTC

Downstream 100 bases:

>100_bases
GCTGCGATCGCTAAACCACCGTTCTTAGATCAATCACCTATCAAGCCACACCATGCAATCGACTTCCCAAGCACGTGATC
TTTTCTCTCGCTTTGTCAAC

Product: ferredoxin-nitrite reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 542; Mature: 542

Protein sequence:

>542_residues
MVISSPAKQFLQGKKLNKIEQNKAFKDGLDVGDELENFAQLGWEQVDKTDLELRLKWYGMFWRPKTPGKFMLRLRIPNGV
LNSHQIKVVASIVGRYGEDGSCDITTRQNLQLRGVLLSDLPEILKRLEEAGLNTIQSGFDNPRNVTGNPLAGIDPLEIVD
TRPYTTELQNFLTNQGKGNHEFSNLPRKWNTAVAGARDNFLLHNDIIFHPVEHDGVMGFGVWLGGILSSQMNDYALPLNA
WVKPNEICRLTGTILTLWRDNGERNNRPKGRFRMYLDSVGIEAFRTMVVEKFGPLTPDPGSVFEDKPRSFYGIHPQKQTG
LHYAGMHVPVGRLNAEDLQDLASASLNYGSGEIRLTEDQNIILVGLSTTNLDKFQADPLLQRFPLEPGSIAAGTVSCTGN
TYCSFALTNTKDQAMAMAHELDAELILPEEIKVHWTGCPNSCGQAYMGAIGLTGTKARAPNGGQGMVEAYDLSLEGRQGP
DAQIGTLHRKGIPGSELKDVLRELLIERFGAQPRSQPKQNSDPFSNFMNWFSNLGENSSPSP

Sequences:

>Translated_542_residues
MVISSPAKQFLQGKKLNKIEQNKAFKDGLDVGDELENFAQLGWEQVDKTDLELRLKWYGMFWRPKTPGKFMLRLRIPNGV
LNSHQIKVVASIVGRYGEDGSCDITTRQNLQLRGVLLSDLPEILKRLEEAGLNTIQSGFDNPRNVTGNPLAGIDPLEIVD
TRPYTTELQNFLTNQGKGNHEFSNLPRKWNTAVAGARDNFLLHNDIIFHPVEHDGVMGFGVWLGGILSSQMNDYALPLNA
WVKPNEICRLTGTILTLWRDNGERNNRPKGRFRMYLDSVGIEAFRTMVVEKFGPLTPDPGSVFEDKPRSFYGIHPQKQTG
LHYAGMHVPVGRLNAEDLQDLASASLNYGSGEIRLTEDQNIILVGLSTTNLDKFQADPLLQRFPLEPGSIAAGTVSCTGN
TYCSFALTNTKDQAMAMAHELDAELILPEEIKVHWTGCPNSCGQAYMGAIGLTGTKARAPNGGQGMVEAYDLSLEGRQGP
DAQIGTLHRKGIPGSELKDVLRELLIERFGAQPRSQPKQNSDPFSNFMNWFSNLGENSSPSP
>Mature_542_residues
MVISSPAKQFLQGKKLNKIEQNKAFKDGLDVGDELENFAQLGWEQVDKTDLELRLKWYGMFWRPKTPGKFMLRLRIPNGV
LNSHQIKVVASIVGRYGEDGSCDITTRQNLQLRGVLLSDLPEILKRLEEAGLNTIQSGFDNPRNVTGNPLAGIDPLEIVD
TRPYTTELQNFLTNQGKGNHEFSNLPRKWNTAVAGARDNFLLHNDIIFHPVEHDGVMGFGVWLGGILSSQMNDYALPLNA
WVKPNEICRLTGTILTLWRDNGERNNRPKGRFRMYLDSVGIEAFRTMVVEKFGPLTPDPGSVFEDKPRSFYGIHPQKQTG
LHYAGMHVPVGRLNAEDLQDLASASLNYGSGEIRLTEDQNIILVGLSTTNLDKFQADPLLQRFPLEPGSIAAGTVSCTGN
TYCSFALTNTKDQAMAMAHELDAELILPEEIKVHWTGCPNSCGQAYMGAIGLTGTKARAPNGGQGMVEAYDLSLEGRQGP
DAQIGTLHRKGIPGSELKDVLRELLIERFGAQPRSQPKQNSDPFSNFMNWFSNLGENSSPSP

Specific function: This Enzyme Catalyzes The 6-Electron Reduction Of Sulfite To Sulfide. This Is One Of Several Activities Required For The Biosynthesis Of L-Cysteine From Sulfate. [C]

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Escherichia coli, GI1789122, Length=524, Percent_Identity=24.0458015267176, Blast_Score=101, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6322597, Length=349, Percent_Identity=25.5014326647564, Blast_Score=84, Evalue=7e-17,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: =1.7.7.1 [H]

Molecular weight: Translated: 60049; Mature: 60049

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVISSPAKQFLQGKKLNKIEQNKAFKDGLDVGDELENFAQLGWEQVDKTDLELRLKWYGM
CCCCCHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCHHHCCCCCEEEEEEEEEE
FWRPKTPGKFMLRLRIPNGVLNSHQIKVVASIVGRYGEDGSCDITTRQNLQLRGVLLSDL
EECCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCEEEEEHHHHH
PEILKRLEEAGLNTIQSGFDNPRNVTGNPLAGIDPLEIVDTRPYTTELQNFLTNQGKGNH
HHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCCHHEECCCCCHHHHHHHHHCCCCCCC
EFSNLPRKWNTAVAGARDNFLLHNDIIFHPVEHDGVMGFGVWLGGILSSQMNDYALPLNA
HHHCCCCHHHHHHCCCCCCEEEECCEEEEECCCCCEEEHHHHHHHHHHHCCCCEECCCCC
WVKPNEICRLTGTILTLWRDNGERNNRPKGRFRMYLDSVGIEAFRTMVVEKFGPLTPDPG
CCCCCHHHHHEEEEEEEECCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCC
SVFEDKPRSFYGIHPQKQTGLHYAGMHVPVGRLNAEDLQDLASASLNYGSGEIRLTEDQN
CCCCCCCCCEECCCCCHHCCCEEECEECCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCC
IILVGLSTTNLDKFQADPLLQRFPLEPGSIAAGTVSCTGNTYCSFALTNTKDQAMAMAHE
EEEEEECCCCCCHHHCCHHHHHCCCCCCCEEEEEEEECCCCEEEEEECCCHHHHHHHHHH
LDAELILPEEIKVHWTGCPNSCGQAYMGAIGLTGTKARAPNGGQGMVEAYDLSLEGRQGP
CCCCEECCCEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCCCCC
DAQIGTLHRKGIPGSELKDVLRELLIERFGAQPRSQPKQNSDPFSNFMNWFSNLGENSSP
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCC
SP
CC
>Mature Secondary Structure
MVISSPAKQFLQGKKLNKIEQNKAFKDGLDVGDELENFAQLGWEQVDKTDLELRLKWYGM
CCCCCHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCHHHCCCCCEEEEEEEEEE
FWRPKTPGKFMLRLRIPNGVLNSHQIKVVASIVGRYGEDGSCDITTRQNLQLRGVLLSDL
EECCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCEEEEEHHHHH
PEILKRLEEAGLNTIQSGFDNPRNVTGNPLAGIDPLEIVDTRPYTTELQNFLTNQGKGNH
HHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCCHHEECCCCCHHHHHHHHHCCCCCCC
EFSNLPRKWNTAVAGARDNFLLHNDIIFHPVEHDGVMGFGVWLGGILSSQMNDYALPLNA
HHHCCCCHHHHHHCCCCCCEEEECCEEEEECCCCCEEEHHHHHHHHHHHCCCCEECCCCC
WVKPNEICRLTGTILTLWRDNGERNNRPKGRFRMYLDSVGIEAFRTMVVEKFGPLTPDPG
CCCCCHHHHHEEEEEEEECCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCC
SVFEDKPRSFYGIHPQKQTGLHYAGMHVPVGRLNAEDLQDLASASLNYGSGEIRLTEDQN
CCCCCCCCCEECCCCCHHCCCEEECEECCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCC
IILVGLSTTNLDKFQADPLLQRFPLEPGSIAAGTVSCTGNTYCSFALTNTKDQAMAMAHE
EEEEEECCCCCCHHHCCHHHHHCCCCCCCEEEEEEEECCCCEEEEEECCCHHHHHHHHHH
LDAELILPEEIKVHWTGCPNSCGQAYMGAIGLTGTKARAPNGGQGMVEAYDLSLEGRQGP
CCCCEECCCEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCCCCC
DAQIGTLHRKGIPGSELKDVLRELLIERFGAQPRSQPKQNSDPFSNFMNWFSNLGENSSP
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCC
SP
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8490140 [H]