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The map label for this gene is cobA [H]

Identifier: 33864501

GI number: 33864501

Start: 2362876

End: 2363661

Strand: Reverse

Name: cobA [H]

Synonym: PMT2237

Alternate gene names: 33864501

Gene position: 2363661-2362876 (Counterclockwise)

Preceding gene: 33864502

Following gene: 33864496

Centisome position: 98.04

GC content: 52.16

Gene sequence:

>786_bases
ATGAGCTCTTTGGACTTTGGCACTGTGTATTTGGTGGGTGCAGGCCCTGGTGATCCTGATTTGCTCACACTGAAGGCTTA
TCGTCTGCTTCGCCAGTGCGGAGCCTTGGTTCACGATGCTCTGGTGCCTAAGGAAGTACTCGAGTTAGTGCCCGAGTCTT
GTGAACGGCATTTTGTTGGTAAACGTCGAGAACGTCATTGGGTTCCACAGTCCAAGACAAATGAGTTGCTTGTTGAATTG
GCTCAGAGTCATTCCTGTGTAGTACGCCTAAAAGGTGGTGATCCTTTTTTGTTTGGGCGGGGTGGTGAGGAGGCCGCCTA
TTTGCAGAGTCATGGGGTATCGGTGGAGGTGGTCCCTGGGATTACAGCGGGGATGGCTGCGCCGGCCTATGTGGGTATTC
CTCTGACCCATCGTTGTGGTGGTAGTTCTGTCACCTTTGTGACAGGTCACGAGAAGATCGACAAACAGCGCCCTTCTGTG
AATTGGAAGGATCTTGCTAGCGCAAGTGACAGCCTGGTGATTTATATGGGTTTGCATAACCTTGCTTATATCGTCGAAGA
GTTGATTGCCGGTGGACTCGATCCAGCTACGTCCGCAGCAATCATTCAACAGGGCACTGTCGTAGGTCAGCGCTACCTCA
AAGCTCCCTTGGAACAGTTGGTTCAAAGGGTGCAGAGCGAAGGGTTTGCAGCGCCGTCGATTGTGATTGTGGGTGCAGTA
GTTGATGAACAGGTTGCAGAGTGTGCTCCTCAGCCGGCTGAGGTTGTGATGCCGATCAAGTCTTGA

Upstream 100 bases:

>100_bases
TCCAGATTACGCAGACGCTGCGCGATTGTGGGGTGATGACTTCCCTACTTGAGATTGAGTTATTTCGATTGGGTTTGATT
GATTTGCTGGCTGCATTGCC

Downstream 100 bases:

>100_bases
TCTATTACTTAGCAACCTAAAGGTAGATGTTGAATTTAGCGTTTAAAGTAGGAGATATCGCTGGGCCATTGGCAGTTCTT
CTGCGGGGTCGCATGTGAGT

Product: uroporphyrin-III C-methyltransferase

Products: NA

Alternate protein names: Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM [H]

Number of amino acids: Translated: 261; Mature: 260

Protein sequence:

>261_residues
MSSLDFGTVYLVGAGPGDPDLLTLKAYRLLRQCGALVHDALVPKEVLELVPESCERHFVGKRRERHWVPQSKTNELLVEL
AQSHSCVVRLKGGDPFLFGRGGEEAAYLQSHGVSVEVVPGITAGMAAPAYVGIPLTHRCGGSSVTFVTGHEKIDKQRPSV
NWKDLASASDSLVIYMGLHNLAYIVEELIAGGLDPATSAAIIQQGTVVGQRYLKAPLEQLVQRVQSEGFAAPSIVIVGAV
VDEQVAECAPQPAEVVMPIKS

Sequences:

>Translated_261_residues
MSSLDFGTVYLVGAGPGDPDLLTLKAYRLLRQCGALVHDALVPKEVLELVPESCERHFVGKRRERHWVPQSKTNELLVEL
AQSHSCVVRLKGGDPFLFGRGGEEAAYLQSHGVSVEVVPGITAGMAAPAYVGIPLTHRCGGSSVTFVTGHEKIDKQRPSV
NWKDLASASDSLVIYMGLHNLAYIVEELIAGGLDPATSAAIIQQGTVVGQRYLKAPLEQLVQRVQSEGFAAPSIVIVGAV
VDEQVAECAPQPAEVVMPIKS
>Mature_260_residues
SSLDFGTVYLVGAGPGDPDLLTLKAYRLLRQCGALVHDALVPKEVLELVPESCERHFVGKRRERHWVPQSKTNELLVELA
QSHSCVVRLKGGDPFLFGRGGEEAAYLQSHGVSVEVVPGITAGMAAPAYVGIPLTHRCGGSSVTFVTGHEKIDKQRPSVN
WKDLASASDSLVIYMGLHNLAYIVEELIAGGLDPATSAAIIQQGTVVGQRYLKAPLEQLVQRVQSEGFAAPSIVIVGAVV
DEQVAECAPQPAEVVMPIKS

Specific function: Catalyzes both methylations at C-2 and C-7 of uroporphyrinogen III leading to precorrin-1 and precorrin-2; their oxidative esterification gives respectively factor I octamethyl ester and sirohydrochlorin [H]

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=235, Percent_Identity=45.9574468085106, Blast_Score=196, Evalue=1e-51,
Organism=Saccharomyces cerevisiae, GI6322922, Length=240, Percent_Identity=30.8333333333333, Blast_Score=109, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: =2.1.1.107 [H]

Molecular weight: Translated: 27899; Mature: 27768

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSLDFGTVYLVGAGPGDPDLLTLKAYRLLRQCGALVHDALVPKEVLELVPESCERHFVG
CCCCCCCEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
KRRERHWVPQSKTNELLVELAQSHSCVVRLKGGDPFLFGRGGEEAAYLQSHGVSVEVVPG
CHHHHCCCCCCCHHHHHHHHHCCCCEEEEECCCCEEEEECCCCCHHHHHHCCCEEEECCC
ITAGMAAPAYVGIPLTHRCGGSSVTFVTGHEKIDKQRPSVNWKDLASASDSLVIYMGLHN
CHHCCCCCEEECCCEEECCCCCEEEEEECHHHHHHCCCCCCHHHHHCCCCCEEEEECHHH
LAYIVEELIAGGLDPATSAAIIQQGTVVGQRYLKAPLEQLVQRVQSEGFAAPSIVIVGAV
HHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHH
VDEQVAECAPQPAEVVMPIKS
HHHHHHHHCCCCCCEEEECCC
>Mature Secondary Structure 
SSLDFGTVYLVGAGPGDPDLLTLKAYRLLRQCGALVHDALVPKEVLELVPESCERHFVG
CCCCCCEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
KRRERHWVPQSKTNELLVELAQSHSCVVRLKGGDPFLFGRGGEEAAYLQSHGVSVEVVPG
CHHHHCCCCCCCHHHHHHHHHCCCCEEEEECCCCEEEEECCCCCHHHHHHCCCEEEECCC
ITAGMAAPAYVGIPLTHRCGGSSVTFVTGHEKIDKQRPSVNWKDLASASDSLVIYMGLHN
CHHCCCCCEEECCCEEECCCCCEEEEEECHHHHHHCCCCCCHHHHHCCCCCEEEEECHHH
LAYIVEELIAGGLDPATSAAIIQQGTVVGQRYLKAPLEQLVQRVQSEGFAAPSIVIVGAV
HHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHH
VDEQVAECAPQPAEVVMPIKS
HHHHHHHHCCCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]