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The map label for this gene is eco

Identifier: 33864485

GI number: 33864485

Start: 2348807

End: 2349340

Strand: Reverse

Name: eco

Synonym: PMT2221

Alternate gene names: 33864485

Gene position: 2349340-2348807 (Counterclockwise)

Preceding gene: 33864489

Following gene: 33864473

Centisome position: 97.45

GC content: 52.06

Gene sequence:

>534_bases
ATGCAGGCATCAATTCAGAACAGAATCTTTTTCGGGCTGGTTGTTTTGTGGTCGACGACAGTGTTGGAACCCCTGCGGGC
AATACCTCGAATGGATTTGAATGATTACCCGCAGCCGATTGCTGGACATCAACGCTGGGTGATTCAGCTCCCAGGGTTGC
TAGCAAAAAGTTCTGATCCTGGCCTGTCCACTAATGCTGTGGATTGGCGGGTCCAGTTGATTGTGGGTCGAACGATCCAA
TTGGTCTGCAATCAGTATCACTTGGCTGGTCAAGGGCTGAGGATGGAGCGGTTTCAAGGAGCTGAGCAGCGGATGCTCTA
TAGCGTTGCAGGTGCAGTCAAGGTGATGTCCACCCGCATGGTTTGTCCTCCTGATGAGCCGAAGCGTGAGAGCTTTCTTG
TTCTTGGCAGTAAGCCTTATTTGGTGCCCTATAACGCCTCCTTTCCGATAGTGGTTGATGTACCTGACGGCTTAGAGGTG
CGTTGGCGGTTGTGGAAGGCTGAGATCACTCAGCGGGAGGCAATCAAGCTTTGA

Upstream 100 bases:

>100_bases
CCACTGCCAAGGCAGCGGCAGTGGCGGAATCAAGACCTCCTGATAGCAGGGCAATGGTTGTTGAGTGGATCATGCTGGAC
ATTCTCTAGCGCTTGTTGGT

Downstream 100 bases:

>100_bases
GGTTCAACGTATACCAAGCCACTTGTGGGTTTGCATGCTGAGCCTCCAGTGGGGATGACTTTGTACGTGCTCGAAAGCTA
GTTGTTGACCTCTTGTTGTT

Product: serine protease inhibitor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 177; Mature: 177

Protein sequence:

>177_residues
MQASIQNRIFFGLVVLWSTTVLEPLRAIPRMDLNDYPQPIAGHQRWVIQLPGLLAKSSDPGLSTNAVDWRVQLIVGRTIQ
LVCNQYHLAGQGLRMERFQGAEQRMLYSVAGAVKVMSTRMVCPPDEPKRESFLVLGSKPYLVPYNASFPIVVDVPDGLEV
RWRLWKAEITQREAIKL

Sequences:

>Translated_177_residues
MQASIQNRIFFGLVVLWSTTVLEPLRAIPRMDLNDYPQPIAGHQRWVIQLPGLLAKSSDPGLSTNAVDWRVQLIVGRTIQ
LVCNQYHLAGQGLRMERFQGAEQRMLYSVAGAVKVMSTRMVCPPDEPKRESFLVLGSKPYLVPYNASFPIVVDVPDGLEV
RWRLWKAEITQREAIKL
>Mature_177_residues
MQASIQNRIFFGLVVLWSTTVLEPLRAIPRMDLNDYPQPIAGHQRWVIQLPGLLAKSSDPGLSTNAVDWRVQLIVGRTIQ
LVCNQYHLAGQGLRMERFQGAEQRMLYSVAGAVKVMSTRMVCPPDEPKRESFLVLGSKPYLVPYNASFPIVVDVPDGLEV
RWRLWKAEITQREAIKL

Specific function: General inhibitor of family S1 serine proteases

COG id: COG4574

COG function: function code R; Serine protease inhibitor ecotin

Gene ontology:

Cell location: Periplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the protease inhibitor I11 (ecotin) family

Homologues:

Organism=Escherichia coli, GI1788538, Length=160, Percent_Identity=28.75, Blast_Score=68, Evalue=3e-13,

Paralogues:

None

Copy number: 260 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): ECOT_PROMM (P59839)

Other databases:

- EMBL:   BX548175
- RefSeq:   NP_896045.1
- ProteinModelPortal:   P59839
- SMR:   P59839
- STRING:   P59839
- GeneID:   1727944
- GenomeReviews:   BX548175_GR
- KEGG:   pmt:PMT2221
- NMPDR:   fig|74547.1.peg.2212
- eggNOG:   COG4574
- HOGENOM:   HBG710412
- OMA:   WRVQLIV
- ProtClustDB:   CLSK826322
- BioCyc:   PMAR74547:PMT2221-MONOMER
- HAMAP:   MF_00706
- InterPro:   IPR005658
- InterPro:   IPR023189
- InterPro:   IPR023084
- Gene3D:   G3DSA:2.60.40.550
- PIRSF:   PIRSF006865

Pfam domain/function: PF03974 Ecotin; SSF49772 Prot_inh_ecotin

EC number: NA

Molecular weight: Translated: 20071; Mature: 20071

Theoretical pI: Translated: 9.78; Mature: 9.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQASIQNRIFFGLVVLWSTTVLEPLRAIPRMDLNDYPQPIAGHQRWVIQLPGLLAKSSDP
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCEEECCCCC
GLSTNAVDWRVQLIVGRTIQLVCNQYHLAGQGLRMERFQGAEQRMLYSVAGAVKVMSTRM
CCCCCCEEEEEEEEECCHHEEHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHCCC
VCPPDEPKRESFLVLGSKPYLVPYNASFPIVVDVPDGLEVRWRLWKAEITQREAIKL
CCCCCCCCCCEEEEECCCCEEEEECCCCCEEEECCCCCEEEEEEEEHHHCHHHHCCC
>Mature Secondary Structure
MQASIQNRIFFGLVVLWSTTVLEPLRAIPRMDLNDYPQPIAGHQRWVIQLPGLLAKSSDP
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCEEECCCCC
GLSTNAVDWRVQLIVGRTIQLVCNQYHLAGQGLRMERFQGAEQRMLYSVAGAVKVMSTRM
CCCCCCEEEEEEEEECCHHEEHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHCCC
VCPPDEPKRESFLVLGSKPYLVPYNASFPIVVDVPDGLEVRWRLWKAEITQREAIKL
CCCCCCCCCCEEEEECCCCEEEEECCCCCEEEECCCCCEEEEEEEEHHHCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917642