Click here to switch to the map view.

The map label for this gene is ligA [H]

Identifier: 33864448

GI number: 33864448

Start: 2309318

End: 2311408

Strand: Direct

Name: ligA [H]

Synonym: PMT2184

Alternate gene names: 33864448

Gene position: 2309318-2311408 (Clockwise)

Preceding gene: 33864447

Following gene: 33864449

Centisome position: 95.79

GC content: 54.85

Gene sequence:

>2091_bases
GTGACCCCGACGTCGATAACACCCCCAAAGCGGGCAGCCGAACTACGCCAACTACTCAATCAGGCCGCCCACTCTTACTA
CGTACTTGATACACCAACGATGGAGGATGCCGTCTACGACCGCCTCTATCGCGAGCTACTGAACCTAGAAGCTAAAGATT
CATCGCTACTCACCCCAGACAGCCCCACCCAAAGGATTGGTGGAGCCCCTGCTCAAGGCTTTGCAAGTGTTCAGCATCGC
ATCCCATTACTCAGCCTCGACAATGCTTTCAATGTTGACGAACTAACGGGCTGGTACGTCCGACTCGCCAAGCTGCTTGG
TCATCAAACCAACCCTAAAACGCCTCAACCTGATCTATCGATGGTGGGCGAACTGAAGATCGATGGCAACGCGCTAGCCC
TCAGCTACGAACACGGCGTACTCATACGCGCAGCCACCCGCGGTGATGGCGCTAATGGAGAGGAGATCACAGCCAACGTC
CGCACCATTGCCTCCATTCCGCTGCGATTACAACTCGTGCATCCTCCAGCCTGGCTGGAAGTGCGAGGAGAAGCGTTTAT
CCCGAATAGCACCTTTGCAGACATCAACACTGCCCGAGGAAAACGGGGCGACGTAGCCTTTGCCAACCCCCGCAATGCCT
GTGCCGGCACGTTGCGTCAACTAGATCCCAAGGTGGTGGCCTCACGTCAGCTGGACTTCTTTGCCTACACCCTGCATCTA
CCAGACAACTGGCAAGCGAAAGCTCATGATCCAGCCAGACCCATGAACCAATGGGCAGCATTGCAATGGCTCCAGGCCAT
CGGCTTCAAAGTGAATCCAAACACCGCGTTGCTTGCGGATTTAACTGCGGTAGAAAACTTCTTCAAGCTCTGGGATGAGG
ATCGCCATCAACTTCCATACGCCACCGACGGAGTAGTCGTAAAGCTCAACGACTTACAACTACAAGAAACTGCAGGCTTT
ACTCAAAAGGCCCCACGTTGGGCGATTGCACTCAAATACGCCGCTGAAGAAGCCCCAAGCAAATTGCTAAGACTCACCTG
CCAGGTGGGACGCACAGGCGCCGTAACACCTGTGGCTGAATTCGAAGCGATACCACTAGCAGGTACAAGTGTGAGCCGCG
CCACACTGCACAACGCCAATCGGGTCAGCGAGCTCGATCTCCATAGCGGCGACACCATCGTGGTGCGCAAGGCCGGCGAA
ATCATCCCGGAAGTGATAGGTGTGTTGAGAGAACTACGACCTGCAGGAGCACAGACACTCAAGCTGCCTCATATCTGTCC
GGAATGCCGATCAGACCTAGTGCGGGAAGAAGACGAAGCAGCAACACGCTGTGTCAACAGCAGCTGCCCAGCGATCCTGC
GCGGAACATTACGTCACTGGGTCAGTAAAGGAGCTCTGGATGTAGATGGCCTCGGCAGCAAGCTGATCGAACAGCTGGTA
AACCGCGGTCTAGTGCAATCGATTGCTGACCTCTATGCCCTCGAGGCAAGCCTTCTGGCAAGCATGGATCGTATGGGCAG
TAAATCAACAGCAAACCTGATAACCGCCCTGCAAGCCTCCAAAAGCAAACCCTGGCATAAGCAGCTCTACGGCCTAGGAA
TCCACCATGTCGGCGAAGTCAATGCTAAAGCCCTAGCTAAAAAGTTCCCCAGCGCCGCCACACTGGCCACCGCCGCCTGC
AAGGCACCCGAACAAATCACGTCAGTGTTCGGAGTCGGCAACGAAATTGCGCAGTCACTCCAACAATGGTTTGCCACCCC
CGCCAATCAACAGCTTCTGGCCAAGTTGAAACAGCTAGGTATCTCCCTGGAAGCCAATGAAGACGAGCTCGCTCACGACA
CAGATCAAAACCAAAGAACGGTCAGCTTGGAGGGCCAAACCTTTGTATTAACAGGCACTCTGCCAACCCTGAGCCGCAGC
GAAGCCCAAGCCCTGATCGAAGCCTCCGGTGGCAAAGTCAATAACTCTGTCAGCAAAAACACCAGTTATGTGGTGGCTGG
AGTTGCAGCGGGCAGCAAACTGAACAAGGCCGAAAAACTTGAAGTCACGGTCCTTAACCAGGAGGAGCTGCTCAAACTTT
TGGCCAGCTGA

Upstream 100 bases:

>100_bases
ATTAAAAATGGATTTCACATTCAGCAAGATTGACGATTCATGGCGAGTCGATCGCATTGTGCCCCTCAATTGATGACCAA
TCGTTCTTAAGACAGCTGCG

Downstream 100 bases:

>100_bases
AGTTCTCCATCCCTAGGCTTTGAATCACTGCATAAGAGCATGAGCCAAGCCAACATCAACACCTGGATCAAAACAGCCTG
CGGACGTGCCAAATATGCAG

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] [H]

Number of amino acids: Translated: 696; Mature: 695

Protein sequence:

>696_residues
MTPTSITPPKRAAELRQLLNQAAHSYYVLDTPTMEDAVYDRLYRELLNLEAKDSSLLTPDSPTQRIGGAPAQGFASVQHR
IPLLSLDNAFNVDELTGWYVRLAKLLGHQTNPKTPQPDLSMVGELKIDGNALALSYEHGVLIRAATRGDGANGEEITANV
RTIASIPLRLQLVHPPAWLEVRGEAFIPNSTFADINTARGKRGDVAFANPRNACAGTLRQLDPKVVASRQLDFFAYTLHL
PDNWQAKAHDPARPMNQWAALQWLQAIGFKVNPNTALLADLTAVENFFKLWDEDRHQLPYATDGVVVKLNDLQLQETAGF
TQKAPRWAIALKYAAEEAPSKLLRLTCQVGRTGAVTPVAEFEAIPLAGTSVSRATLHNANRVSELDLHSGDTIVVRKAGE
IIPEVIGVLRELRPAGAQTLKLPHICPECRSDLVREEDEAATRCVNSSCPAILRGTLRHWVSKGALDVDGLGSKLIEQLV
NRGLVQSIADLYALEASLLASMDRMGSKSTANLITALQASKSKPWHKQLYGLGIHHVGEVNAKALAKKFPSAATLATAAC
KAPEQITSVFGVGNEIAQSLQQWFATPANQQLLAKLKQLGISLEANEDELAHDTDQNQRTVSLEGQTFVLTGTLPTLSRS
EAQALIEASGGKVNNSVSKNTSYVVAGVAAGSKLNKAEKLEVTVLNQEELLKLLAS

Sequences:

>Translated_696_residues
MTPTSITPPKRAAELRQLLNQAAHSYYVLDTPTMEDAVYDRLYRELLNLEAKDSSLLTPDSPTQRIGGAPAQGFASVQHR
IPLLSLDNAFNVDELTGWYVRLAKLLGHQTNPKTPQPDLSMVGELKIDGNALALSYEHGVLIRAATRGDGANGEEITANV
RTIASIPLRLQLVHPPAWLEVRGEAFIPNSTFADINTARGKRGDVAFANPRNACAGTLRQLDPKVVASRQLDFFAYTLHL
PDNWQAKAHDPARPMNQWAALQWLQAIGFKVNPNTALLADLTAVENFFKLWDEDRHQLPYATDGVVVKLNDLQLQETAGF
TQKAPRWAIALKYAAEEAPSKLLRLTCQVGRTGAVTPVAEFEAIPLAGTSVSRATLHNANRVSELDLHSGDTIVVRKAGE
IIPEVIGVLRELRPAGAQTLKLPHICPECRSDLVREEDEAATRCVNSSCPAILRGTLRHWVSKGALDVDGLGSKLIEQLV
NRGLVQSIADLYALEASLLASMDRMGSKSTANLITALQASKSKPWHKQLYGLGIHHVGEVNAKALAKKFPSAATLATAAC
KAPEQITSVFGVGNEIAQSLQQWFATPANQQLLAKLKQLGISLEANEDELAHDTDQNQRTVSLEGQTFVLTGTLPTLSRS
EAQALIEASGGKVNNSVSKNTSYVVAGVAAGSKLNKAEKLEVTVLNQEELLKLLAS
>Mature_695_residues
TPTSITPPKRAAELRQLLNQAAHSYYVLDTPTMEDAVYDRLYRELLNLEAKDSSLLTPDSPTQRIGGAPAQGFASVQHRI
PLLSLDNAFNVDELTGWYVRLAKLLGHQTNPKTPQPDLSMVGELKIDGNALALSYEHGVLIRAATRGDGANGEEITANVR
TIASIPLRLQLVHPPAWLEVRGEAFIPNSTFADINTARGKRGDVAFANPRNACAGTLRQLDPKVVASRQLDFFAYTLHLP
DNWQAKAHDPARPMNQWAALQWLQAIGFKVNPNTALLADLTAVENFFKLWDEDRHQLPYATDGVVVKLNDLQLQETAGFT
QKAPRWAIALKYAAEEAPSKLLRLTCQVGRTGAVTPVAEFEAIPLAGTSVSRATLHNANRVSELDLHSGDTIVVRKAGEI
IPEVIGVLRELRPAGAQTLKLPHICPECRSDLVREEDEAATRCVNSSCPAILRGTLRHWVSKGALDVDGLGSKLIEQLVN
RGLVQSIADLYALEASLLASMDRMGSKSTANLITALQASKSKPWHKQLYGLGIHHVGEVNAKALAKKFPSAATLATAACK
APEQITSVFGVGNEIAQSLQQWFATPANQQLLAKLKQLGISLEANEDELAHDTDQNQRTVSLEGQTFVLTGTLPTLSRSE
AQALIEASGGKVNNSVSKNTSYVVAGVAAGSKLNKAEKLEVTVLNQEELLKLLAS

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain [H]

Homologues:

Organism=Escherichia coli, GI1788750, Length=684, Percent_Identity=41.9590643274854, Blast_Score=518, Evalue=1e-148,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001357
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149 [H]

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 75444; Mature: 75312

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: PS50172 BRCT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPTSITPPKRAAELRQLLNQAAHSYYVLDTPTMEDAVYDRLYRELLNLEAKDSSLLTPD
CCCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCC
SPTQRIGGAPAQGFASVQHRIPLLSLDNAFNVDELTGWYVRLAKLLGHQTNPKTPQPDLS
CCHHHCCCCCCHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHH
MVGELKIDGNALALSYEHGVLIRAATRGDGANGEEITANVRTIASIPLRLQLVHPPAWLE
EEEEEEECCCEEEEEECCCEEEEEECCCCCCCCCEEEEHHHEEEECCEEEEEECCCCCEE
VRGEAFIPNSTFADINTARGKRGDVAFANPRNACAGTLRQLDPKVVASRQLDFFAYTLHL
ECCCEECCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHCCHHHHHHCCEEEEEEEEEC
PDNWQAKAHDPARPMNQWAALQWLQAIGFKVNPNTALLADLTAVENFFKLWDEDRHQLPY
CCCCCCCCCCCCCHHHHHHHHHHHHHCCEEECCCCEEEHHHHHHHHHHHHHCCCCCCCCC
ATDGVVVKLNDLQLQETAGFTQKAPRWAIALKYAAEEAPSKLLRLTCQVGRTGAVTPVAE
CCCCEEEEEECEEEHHHCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
FEAIPLAGTSVSRATLHNANRVSELDLHSGDTIVVRKAGEIIPEVIGVLRELRPAGAQTL
CCCCCCCCCCCHHHHHCCCCCCCEEECCCCCEEEEECHHHHHHHHHHHHHHHCCCCCCEE
KLPHICPECRSDLVREEDEAATRCVNSSCPAILRGTLRHWVSKGALDVDGLGSKLIEQLV
ECCCCCHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
NRGLVQSIADLYALEASLLASMDRMGSKSTANLITALQASKSKPWHKQLYGLGIHHVGEV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHCCCHHCCCC
NAKALAKKFPSAATLATAACKAPEQITSVFGVGNEIAQSLQQWFATPANQQLLAKLKQLG
CHHHHHHHCCCHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHC
ISLEANEDELAHDTDQNQRTVSLEGQTFVLTGTLPTLSRSEAQALIEASGGKVNNSVSKN
CEEECCCHHHCCCCCCCCEEEEECCCEEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCC
TSYVVAGVAAGSKLNKAEKLEVTVLNQEELLKLLAS
CCEEEEEEECCCCCCCCCEEEEEEECHHHHHHHHCC
>Mature Secondary Structure 
TPTSITPPKRAAELRQLLNQAAHSYYVLDTPTMEDAVYDRLYRELLNLEAKDSSLLTPD
CCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCC
SPTQRIGGAPAQGFASVQHRIPLLSLDNAFNVDELTGWYVRLAKLLGHQTNPKTPQPDLS
CCHHHCCCCCCHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHH
MVGELKIDGNALALSYEHGVLIRAATRGDGANGEEITANVRTIASIPLRLQLVHPPAWLE
EEEEEEECCCEEEEEECCCEEEEEECCCCCCCCCEEEEHHHEEEECCEEEEEECCCCCEE
VRGEAFIPNSTFADINTARGKRGDVAFANPRNACAGTLRQLDPKVVASRQLDFFAYTLHL
ECCCEECCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHCCHHHHHHCCEEEEEEEEEC
PDNWQAKAHDPARPMNQWAALQWLQAIGFKVNPNTALLADLTAVENFFKLWDEDRHQLPY
CCCCCCCCCCCCCHHHHHHHHHHHHHCCEEECCCCEEEHHHHHHHHHHHHHCCCCCCCCC
ATDGVVVKLNDLQLQETAGFTQKAPRWAIALKYAAEEAPSKLLRLTCQVGRTGAVTPVAE
CCCCEEEEEECEEEHHHCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
FEAIPLAGTSVSRATLHNANRVSELDLHSGDTIVVRKAGEIIPEVIGVLRELRPAGAQTL
CCCCCCCCCCCHHHHHCCCCCCCEEECCCCCEEEEECHHHHHHHHHHHHHHHCCCCCCEE
KLPHICPECRSDLVREEDEAATRCVNSSCPAILRGTLRHWVSKGALDVDGLGSKLIEQLV
ECCCCCHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
NRGLVQSIADLYALEASLLASMDRMGSKSTANLITALQASKSKPWHKQLYGLGIHHVGEV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHCCCHHCCCC
NAKALAKKFPSAATLATAACKAPEQITSVFGVGNEIAQSLQQWFATPANQQLLAKLKQLG
CHHHHHHHCCCHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHC
ISLEANEDELAHDTDQNQRTVSLEGQTFVLTGTLPTLSRSEAQALIEASGGKVNNSVSKN
CEEECCCHHHCCCCCCCCEEEEECCCEEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCC
TSYVVAGVAAGSKLNKAEKLEVTVLNQEELLKLLAS
CCEEEEEEECCCCCCCCCEEEEEEECHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA