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The map label for this gene is gcsH

Identifier: 33864434

GI number: 33864434

Start: 2297069

End: 2297467

Strand: Reverse

Name: gcsH

Synonym: PMT2170

Alternate gene names: 33864434

Gene position: 2297467-2297069 (Counterclockwise)

Preceding gene: 33864435

Following gene: 33864433

Centisome position: 95.3

GC content: 53.88

Gene sequence:

>399_bases
ATGGCTTTCCAATTCCCCGACCACTTTCGGTTTGCCGATACCCATGAATACGCCAGTTTGGATGGTGAATTGGTGCGGAT
AGGCATCAGTGCCTTTGCTGTTGATCAGTTGGGTGACATTGTGTTTGTGGATTTGCCGGAGGTGGGCGATCGGCTGAATC
GGGGCACCACTTTCGGGTCCGTTGAGTCGGTGAAAGCTGTCGAGGATTTGCATGCTCCGATCAGCGGTGAGCTGGTGCGG
ATTAATGAGTCGGTGCTTTCCAGCCCGGATGAACTTCAAAATGATCCTCATGGTGAGGGGTGGTTGCTCGTCGTTCGCCC
GGCGGACCCAGCTCAATTGCAGGATCTGATGGACGCAGCCACCTATGCCAACAAGGTGGCTATCGAATCAAGCAACTAA

Upstream 100 bases:

>100_bases
CTCATGTGGAATTGGCACTGATCCGAGCTTTGGTGGCTTTGGTTCGCGCCGGTTTGCTGGATGTACCCCAGACTGGGTGA
GCTTTGCCTCGCCAGGCTCG

Downstream 100 bases:

>100_bases
GCAGCTGATGAAGTGCGATGGTTCTGGGCCAAATTTTCTAAACTTCTGTCCATCTTGTTGATGAATGTCCCTTGACCCTG
CTTGAACAGCGTGCAGTTGA

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 132; Mature: 131

Protein sequence:

>132_residues
MAFQFPDHFRFADTHEYASLDGELVRIGISAFAVDQLGDIVFVDLPEVGDRLNRGTTFGSVESVKAVEDLHAPISGELVR
INESVLSSPDELQNDPHGEGWLLVVRPADPAQLQDLMDAATYANKVAIESSN

Sequences:

>Translated_132_residues
MAFQFPDHFRFADTHEYASLDGELVRIGISAFAVDQLGDIVFVDLPEVGDRLNRGTTFGSVESVKAVEDLHAPISGELVR
INESVLSSPDELQNDPHGEGWLLVVRPADPAQLQDLMDAATYANKVAIESSN
>Mature_131_residues
AFQFPDHFRFADTHEYASLDGELVRIGISAFAVDQLGDIVFVDLPEVGDRLNRGTTFGSVESVKAVEDLHAPISGELVRI
NESVLSSPDELQNDPHGEGWLLVVRPADPAQLQDLMDAATYANKVAIESSN

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI49574537, Length=113, Percent_Identity=38.0530973451327, Blast_Score=101, Evalue=2e-22,
Organism=Homo sapiens, GI89057342, Length=113, Percent_Identity=38.0530973451327, Blast_Score=100, Evalue=5e-22,
Organism=Escherichia coli, GI1789271, Length=118, Percent_Identity=36.4406779661017, Blast_Score=96, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17551294, Length=122, Percent_Identity=34.4262295081967, Blast_Score=89, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17507493, Length=114, Percent_Identity=33.3333333333333, Blast_Score=86, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6319272, Length=123, Percent_Identity=32.520325203252, Blast_Score=84, Evalue=7e-18,
Organism=Drosophila melanogaster, GI17865652, Length=114, Percent_Identity=34.2105263157895, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH_PROMM (Q7TUJ2)

Other databases:

- EMBL:   BX548175
- RefSeq:   NP_895994.1
- HSSP:   Q9WY55
- ProteinModelPortal:   Q7TUJ2
- SMR:   Q7TUJ2
- STRING:   Q7TUJ2
- GeneID:   1728562
- GenomeReviews:   BX548175_GR
- KEGG:   pmt:PMT2170
- NMPDR:   fig|74547.1.peg.2161
- eggNOG:   COG0509
- HOGENOM:   HBG693789
- OMA:   QGAGWFF
- ProtClustDB:   PRK01202
- BioCyc:   PMAR74547:PMT2170-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053
- PANTHER:   PTHR11715
- TIGRFAMs:   TIGR00527

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 14395; Mature: 14264

Theoretical pI: Translated: 4.04; Mature: 4.04

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFQFPDHFRFADTHEYASLDGELVRIGISAFAVDQLGDIVFVDLPEVGDRLNRGTTFGS
CCCCCCCCCCCCCCCCHHCCCCEEEEEEHHHHHHHHCCCEEEEECCHHHHHHHCCCCCCC
VESVKAVEDLHAPISGELVRINESVLSSPDELQNDPHGEGWLLVVRPADPAQLQDLMDAA
HHHHHHHHHHCCCCCCCEEEECHHHHCCCHHHCCCCCCCCEEEEEECCCHHHHHHHHHHH
TYANKVAIESSN
HHHCEEEEECCC
>Mature Secondary Structure 
AFQFPDHFRFADTHEYASLDGELVRIGISAFAVDQLGDIVFVDLPEVGDRLNRGTTFGS
CCCCCCCCCCCCCCCHHCCCCEEEEEEHHHHHHHHCCCEEEEECCHHHHHHHCCCCCCC
VESVKAVEDLHAPISGELVRINESVLSSPDELQNDPHGEGWLLVVRPADPAQLQDLMDAA
HHHHHHHHHHCCCCCCCEEEECHHHHCCCHHHCCCCCCCCEEEEEECCCHHHHHHHHHHH
TYANKVAIESSN
HHHCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917642