Click here to switch to the map view.

The map label for this gene is spoT [H]

Identifier: 33864375

GI number: 33864375

Start: 2225482

End: 2227812

Strand: Direct

Name: spoT [H]

Synonym: PMT2111

Alternate gene names: 33864375

Gene position: 2225482-2227812 (Clockwise)

Preceding gene: 33864373

Following gene: 33864376

Centisome position: 92.31

GC content: 52.85

Gene sequence:

>2331_bases
ATGCCCAACGTCACCACTGCCCCAAAGTCAACACAGGCCAATGCTGGCTGTGGTTCAGAGGTGTGTGGTTTACCCGAGAT
CAGGACACATCCGTTCAGCAGTGTTGATGACTACGGCATTGCCTTGCCGGAATGGTTGAAAGACTGTATTAAGCACATTC
CTCCTGGGCTTGGGCAGAGTTGCCCAATCGATTCAGAAGCCCTGCTTGCAGCAGCTTTTGATTTGGCCTTTCAGCTACAT
GAGGGGCAATTTCGAGCCAGTGGTGACCCATATATCGTTCACCCCGTGGCAGTCGCTGATCTGTTGCGGGATATCGGCGC
TAGCGCCAGCGTGATCGCTGCAGGTTTCCTTCACGATGTGGTGGAAGACACCGATTTAACCCCAGAGCAACTGGAAGATT
ATTTCGGCGCTGAAGTACGGGAACTGGTGGAAGGCGTCACCAAACTGGGGGGGATTAATTTCACGAACCACACTGAGGCT
CAAGCTGAGAACTTGCGCAAAATGTTTCTGGCAATGGCCAGCGACATCCGCGTGGTGCTGGTGAAATTGGCCGATAGGCT
TCACAACATGCGCACGCTTGGCTCATTGCAGGCTGATAAGCAGCAGCGCATTGCTCGTGAAACCCGCGAGATATATGCAC
CTCTAGCCAATCGCCTCGGCATTGGTCGCTTCAAATGGGAACTCGAGGATTTGGCCTTCAAGCTTCTTGAACCTGAGGCT
TTCCGAGAGATTCAGCAAGAGGTGGCTACCAAGCGCAGTGAACGGGAGAACCGTCTGGCGAACACGGTTGAACTGTTAAC
CGAACGACTGGCCTCTGCGGGGCTTGATACTTGTGAAGTGAGTGGGCGACCAAAGCATCTCTATGGCATCTGGAGCAAGA
TGCAGCGCCAACAAAAAGCCTTCCATGAGATCTTCGATGTAGCGGCTTTGCGGATTATCGCTCCCAGTGTGGAGACGTGT
TACCGCGCCTTGGCTGTCGTGCATGACACCTTCCGGCCTATTCCCGGACGTTTCAAGGATTACATCGGTTTACCAAAGCC
CAATGGCTATCAATCGCTACATACGGCGGTGATCGGCAAGCATCGGCCCATTGAGGTGCAGATTCGTACTCCAGGGATGC
ATCGTGTGTCTGAATTCGGCATTGCAGCTCATTGGGAGTACAAAGAAGGTGGATCCCCAGCGGCCGCTAATAAGGAGCGG
TTTAATTGGCTTCGTCAGCTGGTGGAGTGGCAACAAGAAGGAGGCAATGATGACCACAACGACTACCTGGCTTCGATCAA
AGAAGACTTGTTTGATGAGGAGGTGTTTGTATTTACTCCTAAAGGCGATGTAGTGGGCTTGCGCAAAGACTCAACAGTTG
TTGATTTCGCCTATCGGATTCATTCAGAGGTTGGCAACCATTGCCATGGTGCGCGCATTAATGATCGTCTTTGTCCATTG
TCTACCTCATTACAGAACGGTGATTTCGTGCAAGTTCTCACCAGCAAAACAGCGCATCCAAGCCTCGACTGGCTGAACTT
TGTGGCTACTCCCACAGCCCGTAATCGCATCCGTCAGTGGTATAAGCGCAGCCACCGTAATGAAACGATCCAACGGGGGA
AAGACCTGTTGGAGCGTGAACTTGGTCGCAATGGTTTTGATGCGCTACTCAATAGTGAAGCGATGACACGCGTAGCAGAG
CGCTGCAACTTGACGGTTACAGAGGATTTGCTGGCGGCGCTTGGTTTTGGAGCCTTGACATTGCAGCAAGTGCTGAATCG
TTTGCGAGAAGAAATCCGTCTTCAGACTATCGAGCCTGAGCAACCACTTAGCAATGTCAATGTTGCCAAACGGCTTGTGG
CTCAAGTGGAGTCGAGCCCAGCCAGGCTGCCTACTTCTGATGGCGTACCGATCCTTGGCGTGGAGGGCCTTGATTACCGC
CTGGGCGGCTGTTGTAGTCCGCTGCCAGGAGAAGCGATCCTTGGCACGGTTGCTCTTGGTAATCACGGCATCACAATTCA
CCGGCAGGATTGCGTCAATATTGGCTCGGTCCCCAGTGAAAGACGATTGCCGGTGCGCTGGAATCAAGGTGCCTTCATCG
AAAACGAGCGATTCCCGGTGCAGTTGCGCATTGAAGTGATTGACCGGGTGGGAATCCTGAAAGACATCCTGATGCGTCTT
TCCGATAACCACATCAATGTGAGCGATGCCAGGGTGAAAACGTCCTATGGCAAACCCGCCTGCATTGATTTGCGGGTTGA
GCTCAACAGTGCTGCTCAGCTGGTCTCAATGATGGATCAGATTCGCTCGATGGCCGATGTTCTCGATATCGCCCGCACTG
GCTTGAGCTAG

Upstream 100 bases:

>100_bases
GCGCAGTTGGCTGAGCTCGAGTACCTGCTCTGAAGAGCTGGGCATCGTTAGTAGCACGGAGTCTTGAGACTGAATACCAT
GGATTGATCCGCTGGGTCGG

Downstream 100 bases:

>100_bases
GGGAGAACGATATAAGCTGGTAAAAGCCTACTGGGATGTCTCTTAGTGGCTATGCCTTTATTGAGTGAGTTTTGTCTATA
CGTCTACTGCCATGAGGCAT

Product: guanosine-3',5'-bis(diphosphate) 3'-diphosphatase, (ppGpp)ase

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 776; Mature: 775

Protein sequence:

>776_residues
MPNVTTAPKSTQANAGCGSEVCGLPEIRTHPFSSVDDYGIALPEWLKDCIKHIPPGLGQSCPIDSEALLAAAFDLAFQLH
EGQFRASGDPYIVHPVAVADLLRDIGASASVIAAGFLHDVVEDTDLTPEQLEDYFGAEVRELVEGVTKLGGINFTNHTEA
QAENLRKMFLAMASDIRVVLVKLADRLHNMRTLGSLQADKQQRIARETREIYAPLANRLGIGRFKWELEDLAFKLLEPEA
FREIQQEVATKRSERENRLANTVELLTERLASAGLDTCEVSGRPKHLYGIWSKMQRQQKAFHEIFDVAALRIIAPSVETC
YRALAVVHDTFRPIPGRFKDYIGLPKPNGYQSLHTAVIGKHRPIEVQIRTPGMHRVSEFGIAAHWEYKEGGSPAAANKER
FNWLRQLVEWQQEGGNDDHNDYLASIKEDLFDEEVFVFTPKGDVVGLRKDSTVVDFAYRIHSEVGNHCHGARINDRLCPL
STSLQNGDFVQVLTSKTAHPSLDWLNFVATPTARNRIRQWYKRSHRNETIQRGKDLLERELGRNGFDALLNSEAMTRVAE
RCNLTVTEDLLAALGFGALTLQQVLNRLREEIRLQTIEPEQPLSNVNVAKRLVAQVESSPARLPTSDGVPILGVEGLDYR
LGGCCSPLPGEAILGTVALGNHGITIHRQDCVNIGSVPSERRLPVRWNQGAFIENERFPVQLRIEVIDRVGILKDILMRL
SDNHINVSDARVKTSYGKPACIDLRVELNSAAQLVSMMDQIRSMADVLDIARTGLS

Sequences:

>Translated_776_residues
MPNVTTAPKSTQANAGCGSEVCGLPEIRTHPFSSVDDYGIALPEWLKDCIKHIPPGLGQSCPIDSEALLAAAFDLAFQLH
EGQFRASGDPYIVHPVAVADLLRDIGASASVIAAGFLHDVVEDTDLTPEQLEDYFGAEVRELVEGVTKLGGINFTNHTEA
QAENLRKMFLAMASDIRVVLVKLADRLHNMRTLGSLQADKQQRIARETREIYAPLANRLGIGRFKWELEDLAFKLLEPEA
FREIQQEVATKRSERENRLANTVELLTERLASAGLDTCEVSGRPKHLYGIWSKMQRQQKAFHEIFDVAALRIIAPSVETC
YRALAVVHDTFRPIPGRFKDYIGLPKPNGYQSLHTAVIGKHRPIEVQIRTPGMHRVSEFGIAAHWEYKEGGSPAAANKER
FNWLRQLVEWQQEGGNDDHNDYLASIKEDLFDEEVFVFTPKGDVVGLRKDSTVVDFAYRIHSEVGNHCHGARINDRLCPL
STSLQNGDFVQVLTSKTAHPSLDWLNFVATPTARNRIRQWYKRSHRNETIQRGKDLLERELGRNGFDALLNSEAMTRVAE
RCNLTVTEDLLAALGFGALTLQQVLNRLREEIRLQTIEPEQPLSNVNVAKRLVAQVESSPARLPTSDGVPILGVEGLDYR
LGGCCSPLPGEAILGTVALGNHGITIHRQDCVNIGSVPSERRLPVRWNQGAFIENERFPVQLRIEVIDRVGILKDILMRL
SDNHINVSDARVKTSYGKPACIDLRVELNSAAQLVSMMDQIRSMADVLDIARTGLS
>Mature_775_residues
PNVTTAPKSTQANAGCGSEVCGLPEIRTHPFSSVDDYGIALPEWLKDCIKHIPPGLGQSCPIDSEALLAAAFDLAFQLHE
GQFRASGDPYIVHPVAVADLLRDIGASASVIAAGFLHDVVEDTDLTPEQLEDYFGAEVRELVEGVTKLGGINFTNHTEAQ
AENLRKMFLAMASDIRVVLVKLADRLHNMRTLGSLQADKQQRIARETREIYAPLANRLGIGRFKWELEDLAFKLLEPEAF
REIQQEVATKRSERENRLANTVELLTERLASAGLDTCEVSGRPKHLYGIWSKMQRQQKAFHEIFDVAALRIIAPSVETCY
RALAVVHDTFRPIPGRFKDYIGLPKPNGYQSLHTAVIGKHRPIEVQIRTPGMHRVSEFGIAAHWEYKEGGSPAAANKERF
NWLRQLVEWQQEGGNDDHNDYLASIKEDLFDEEVFVFTPKGDVVGLRKDSTVVDFAYRIHSEVGNHCHGARINDRLCPLS
TSLQNGDFVQVLTSKTAHPSLDWLNFVATPTARNRIRQWYKRSHRNETIQRGKDLLERELGRNGFDALLNSEAMTRVAER
CNLTVTEDLLAALGFGALTLQQVLNRLREEIRLQTIEPEQPLSNVNVAKRLVAQVESSPARLPTSDGVPILGVEGLDYRL
GGCCSPLPGEAILGTVALGNHGITIHRQDCVNIGSVPSERRLPVRWNQGAFIENERFPVQLRIEVIDRVGILKDILMRLS
DNHINVSDARVKTSYGKPACIDLRVELNSAAQLVSMMDQIRSMADVLDIARTGLS

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Homo sapiens, GI38348360, Length=132, Percent_Identity=39.3939393939394, Blast_Score=73, Evalue=1e-12,
Organism=Escherichia coli, GI1790082, Length=715, Percent_Identity=39.8601398601399, Blast_Score=523, Evalue=1e-149,
Organism=Escherichia coli, GI1789147, Length=716, Percent_Identity=34.7765363128492, Blast_Score=439, Evalue=1e-124,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 86467; Mature: 86336

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNVTTAPKSTQANAGCGSEVCGLPEIRTHPFSSVDDYGIALPEWLKDCIKHIPPGLGQS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCC
CPIDSEALLAAAFDLAFQLHEGQFRASGDPYIVHPVAVADLLRDIGASASVIAAGFLHDV
CCCCHHHHHHHHHHHHHHHCCCCEECCCCCEEEHHHHHHHHHHHHCCCHHHHHHHHHHHH
VEDTDLTPEQLEDYFGAEVRELVEGVTKLGGINFTNHTEAQAENLRKMFLAMASDIRVVL
HHCCCCCHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VKLADRLHNMRTLGSLQADKQQRIARETREIYAPLANRLGIGRFKWELEDLAFKLLEPEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHH
FREIQQEVATKRSERENRLANTVELLTERLASAGLDTCEVSGRPKHLYGIWSKMQRQQKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHH
FHEIFDVAALRIIAPSVETCYRALAVVHDTFRPIPGRFKDYIGLPKPNGYQSLHTAVIGK
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCHHHHHHHHHCC
HRPIEVQIRTPGMHRVSEFGIAAHWEYKEGGSPAAANKERFNWLRQLVEWQQEGGNDDHN
CCCEEEEEECCCHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHH
DYLASIKEDLFDEEVFVFTPKGDVVGLRKDSTVVDFAYRIHSEVGNHCHGARINDRLCPL
HHHHHHHHHHCCCCEEEECCCCCEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
STSLQNGDFVQVLTSKTAHPSLDWLNFVATPTARNRIRQWYKRSHRNETIQRGKDLLERE
CCCCCCCCEEEEECCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHH
LGRNGFDALLNSEAMTRVAERCNLTVTEDLLAALGFGALTLQQVLNRLREEIRLQTIEPE
HCCCHHHHHHCHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCC
QPLSNVNVAKRLVAQVESSPARLPTSDGVPILGVEGLDYRLGGCCSPLPGEAILGTVALG
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCHHHEEEEEEC
NHGITIHRQDCVNIGSVPSERRLPVRWNQGAFIENERFPVQLRIEVIDRVGILKDILMRL
CCCEEEEHHHCCCCCCCCCCCCCCEEECCCCEEECCCCCEEEHHHHHHHHHHHHHHHHHH
SDNHINVSDARVKTSYGKPACIDLRVELNSAAQLVSMMDQIRSMADVLDIARTGLS
CCCCCCCCCCEEEECCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PNVTTAPKSTQANAGCGSEVCGLPEIRTHPFSSVDDYGIALPEWLKDCIKHIPPGLGQS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCC
CPIDSEALLAAAFDLAFQLHEGQFRASGDPYIVHPVAVADLLRDIGASASVIAAGFLHDV
CCCCHHHHHHHHHHHHHHHCCCCEECCCCCEEEHHHHHHHHHHHHCCCHHHHHHHHHHHH
VEDTDLTPEQLEDYFGAEVRELVEGVTKLGGINFTNHTEAQAENLRKMFLAMASDIRVVL
HHCCCCCHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VKLADRLHNMRTLGSLQADKQQRIARETREIYAPLANRLGIGRFKWELEDLAFKLLEPEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHH
FREIQQEVATKRSERENRLANTVELLTERLASAGLDTCEVSGRPKHLYGIWSKMQRQQKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHH
FHEIFDVAALRIIAPSVETCYRALAVVHDTFRPIPGRFKDYIGLPKPNGYQSLHTAVIGK
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCHHHHHHHHHCC
HRPIEVQIRTPGMHRVSEFGIAAHWEYKEGGSPAAANKERFNWLRQLVEWQQEGGNDDHN
CCCEEEEEECCCHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHH
DYLASIKEDLFDEEVFVFTPKGDVVGLRKDSTVVDFAYRIHSEVGNHCHGARINDRLCPL
HHHHHHHHHHCCCCEEEECCCCCEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
STSLQNGDFVQVLTSKTAHPSLDWLNFVATPTARNRIRQWYKRSHRNETIQRGKDLLERE
CCCCCCCCEEEEECCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHH
LGRNGFDALLNSEAMTRVAERCNLTVTEDLLAALGFGALTLQQVLNRLREEIRLQTIEPE
HCCCHHHHHHCHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCC
QPLSNVNVAKRLVAQVESSPARLPTSDGVPILGVEGLDYRLGGCCSPLPGEAILGTVALG
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCHHHEEEEEEC
NHGITIHRQDCVNIGSVPSERRLPVRWNQGAFIENERFPVQLRIEVIDRVGILKDILMRL
CCCEEEEHHHCCCCCCCCCCCCCCEEECCCCEEECCCCCEEEHHHHHHHHHHHHHHHHHH
SDNHINVSDARVKTSYGKPACIDLRVELNSAAQLVSMMDQIRSMADVLDIARTGLS
CCCCCCCCCCEEEECCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]