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The map label for this gene is gloA [H]

Identifier: 33864348

GI number: 33864348

Start: 2198007

End: 2198408

Strand: Direct

Name: gloA [H]

Synonym: PMT2084

Alternate gene names: 33864348

Gene position: 2198007-2198408 (Clockwise)

Preceding gene: 33864346

Following gene: 33864349

Centisome position: 91.17

GC content: 56.72

Gene sequence:

>402_bases
ATGCGGATGTTGCACACGATGCTGAGGGTGGGGGATTTGGATCGTTCTCTGCGCTTCTACACCGAGGTTCTAGGCATGCA
TCTGTTGCGGCGAAAGGACTACCCCTCAGGTCGCTTCACCTTGGCGTTTGTCGGTTATGGCAAGGAGAGTGACACCACTG
TGCTGGAGCTCACACACAACTGGGACCAAGATCACTACGAGTTGGGAGAGGGCTATGGCCATATCGCCTTGGGCGTGGAG
GATATTCAATCCACCTGCTTGGCCATCTCAAAGCGAGGCGGGCGTGTGGTGCGAGAACCCGGGCCTATGCAACATGGCAG
CACCGTGATTGCCTTTGTGGAAGATCCCGATGGCTACAAGGTGGAGCTGATTCAATTGGCCTCTCGTGAGGCCGCGAGCT
GA

Upstream 100 bases:

>100_bases
CGGACTGTGTACCGCCTAGCCCTTTTACTGATGCTGAGGCTGTTGTCAGTCGTAGAACGACGCAGAATGAACTGAGTCTT
AAAGGTCAGGATCGCTCGAG

Downstream 100 bases:

>100_bases
ACCCTATGCATCCTGAGACCAGTCAAGCGGCTTCCGCCCAGGGAAGTCTCACAAGTGATCCTGATCGATTCAGTGATGAC
GCTTGGGATTTGTTGCTTGT

Product: glyoxalase/bleomycin resistance protein/dioxygenase family protein

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 133; Mature: 133

Protein sequence:

>133_residues
MRMLHTMLRVGDLDRSLRFYTEVLGMHLLRRKDYPSGRFTLAFVGYGKESDTTVLELTHNWDQDHYELGEGYGHIALGVE
DIQSTCLAISKRGGRVVREPGPMQHGSTVIAFVEDPDGYKVELIQLASREAAS

Sequences:

>Translated_133_residues
MRMLHTMLRVGDLDRSLRFYTEVLGMHLLRRKDYPSGRFTLAFVGYGKESDTTVLELTHNWDQDHYELGEGYGHIALGVE
DIQSTCLAISKRGGRVVREPGPMQHGSTVIAFVEDPDGYKVELIQLASREAAS
>Mature_133_residues
MRMLHTMLRVGDLDRSLRFYTEVLGMHLLRRKDYPSGRFTLAFVGYGKESDTTVLELTHNWDQDHYELGEGYGHIALGVE
DIQSTCLAISKRGGRVVREPGPMQHGSTVIAFVEDPDGYKVELIQLASREAAS

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=147, Percent_Identity=37.4149659863946, Blast_Score=92, Evalue=2e-19,
Organism=Escherichia coli, GI1787940, Length=125, Percent_Identity=61.6, Blast_Score=168, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6323639, Length=139, Percent_Identity=41.0071942446043, Blast_Score=100, Evalue=9e-23,
Organism=Drosophila melanogaster, GI20129743, Length=150, Percent_Identity=36, Blast_Score=86, Evalue=8e-18,
Organism=Drosophila melanogaster, GI19920404, Length=138, Percent_Identity=30.4347826086957, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 14983; Mature: 14983

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS00934 GLYOXALASE_I_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRMLHTMLRVGDLDRSLRFYTEVLGMHLLRRKDYPSGRFTLAFVGYGKESDTTVLELTHN
CHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCEEEEEECC
WDQDHYELGEGYGHIALGVEDIQSTCLAISKRGGRVVREPGPMQHGSTVIAFVEDPDGYK
CCCCHHHHCCCCCEEEECHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEEECCCCCE
VELIQLASREAAS
EEEEEECCCCCCC
>Mature Secondary Structure
MRMLHTMLRVGDLDRSLRFYTEVLGMHLLRRKDYPSGRFTLAFVGYGKESDTTVLELTHN
CHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCEEEEEECC
WDQDHYELGEGYGHIALGVEDIQSTCLAISKRGGRVVREPGPMQHGSTVIAFVEDPDGYK
CCCCHHHHCCCCCEEEECHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEEECCCCCE
VELIQLASREAAS
EEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]