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The map label for this gene is menE [H]

Identifier: 33864322

GI number: 33864322

Start: 2165964

End: 2167199

Strand: Reverse

Name: menE [H]

Synonym: PMT2057

Alternate gene names: 33864322

Gene position: 2167199-2165964 (Counterclockwise)

Preceding gene: 33864323

Following gene: 33864321

Centisome position: 89.89

GC content: 57.2

Gene sequence:

>1236_bases
ATGAGGGAGTTGGTTGCCCTGCGTTGCAATCCCGCTCAGGCTGAGCAAAGGGCCTTGCAGCTCGCTCAGGCCTTGGAGCA
AGGCGATTGGGTGCAACTGCTGCCGCCTCTTGGAACTAGCGGTTTGATCCCTAGGGCTCTTTTGCCTGATGGTCCTGGTG
TGGTGGTCGCAAGTGGAGGCACCTCCGGCGGACAGCAGCACTGTTTGCAGCCTTGTAGCCATCTCGACTTATCAGCTTCG
GCTACAGGTCAATGGCTTCAGCAGCAGGGTTACGACCTGCAGCAATGCATGGTGCTTAATCCTTTACCGTTGCATCACGT
TAGTGGCTTGATGTCTTGGTGGCGTAGTCGTTGTTGGCGTATTGGGCATACATGGCTACAGCCTGGCTTGATGAGGGATC
CAGACGCTTTAGAGCAGTTGTGTGGCTCGTTGCCGGGATGGGGAGAGCAACTGGTCTTGATGTCTTTGGTGCCAACACAA
CTGCAGCGGTTGTTGACGCATAACGCTGGTGTGCGTGTGTTGAAGGCTTGTGCTGTGATTTGGGTTGGTGGAGCAGTGCT
TTCGCCAGGGTTGATGAAAGCGGCACGGGCCGAAGGCTTGCGCTTGGCACCCTGCTATGGAGCAACTGAGACAGCGGCGA
TGGTGACAGCGCTTGCTCCTGAGGATTTCCTGGCGGGTGCGACGGGCTGTGGATCACCGCTTGAAGATGTGGAGCTGCAG
TTGACGCAAGCAGGCGCGTTGCAAGTTCGAACGGCGCGGTTGGCGTTGGCGCGCTGGTTGGGTGAGGGCTTGGAGGATGT
TCGCGATCAGCAAGGTTGGTGGACCTCTGGAGATGCTGGTTGCTTGATCACTGAGAGCAACCGGGTTTGGCTCAGCATTG
CTGGCCGCTTGGATGGAGCGATTCACTCTGGTGGTGAAACGGTGTTCCCTGAACAGTTGGAGGCCCGTTTACAAGTGGCG
GCGGTTGGTTTGCCTGTTGAGGCTGTGTTGCTGCTTGCTGTTGAAGACAGGGAATGGGGGCAACGCTTGGTGGCGCTTGT
GCGTCCTGCTGCTGGGGCGAATGGAGAGGATTTGATCAAGCAACTACGCCATCTCTGTGCCGATTGGCCGCCTGCCGATC
GGCCGATGCATTGGCGGCTTTGTCCAATGCTTGCTCCGATGGCGAATGGAAAATGGCAACGGGGCTATTGGCAGCAATGG
TTGCGGTTGCTAGAAGCGGAAAGTACAGAGTTTTGA

Upstream 100 bases:

>100_bases
AGCATCAGGGCCCTACACCAGTGGCTCCTGGCCTGGCCCCGGGTTGGTGCCCTGATGGACCTTTATTTAGCGCTGATCCC
GAAGTGGTCTGGGCGGCGGC

Downstream 100 bases:

>100_bases
TTATGGCCAGTCTTTCTGATGCTGAGCTTAGTAATAAGAAATTAGCCGCGGGTTTGCTAGGGATCTTTCTTGGCGCTTTA
GGGATTCATAAATTTGTCTT

Product: O-succinylbenzoic acid--CoA ligase (OSB-CoA synthetase)

Products: NA

Alternate protein names: o-succinylbenzoyl-CoA synthetase; OSB-CoA synthetase [H]

Number of amino acids: Translated: 411; Mature: 411

Protein sequence:

>411_residues
MRELVALRCNPAQAEQRALQLAQALEQGDWVQLLPPLGTSGLIPRALLPDGPGVVVASGGTSGGQQHCLQPCSHLDLSAS
ATGQWLQQQGYDLQQCMVLNPLPLHHVSGLMSWWRSRCWRIGHTWLQPGLMRDPDALEQLCGSLPGWGEQLVLMSLVPTQ
LQRLLTHNAGVRVLKACAVIWVGGAVLSPGLMKAARAEGLRLAPCYGATETAAMVTALAPEDFLAGATGCGSPLEDVELQ
LTQAGALQVRTARLALARWLGEGLEDVRDQQGWWTSGDAGCLITESNRVWLSIAGRLDGAIHSGGETVFPEQLEARLQVA
AVGLPVEAVLLLAVEDREWGQRLVALVRPAAGANGEDLIKQLRHLCADWPPADRPMHWRLCPMLAPMANGKWQRGYWQQW
LRLLEAESTEF

Sequences:

>Translated_411_residues
MRELVALRCNPAQAEQRALQLAQALEQGDWVQLLPPLGTSGLIPRALLPDGPGVVVASGGTSGGQQHCLQPCSHLDLSAS
ATGQWLQQQGYDLQQCMVLNPLPLHHVSGLMSWWRSRCWRIGHTWLQPGLMRDPDALEQLCGSLPGWGEQLVLMSLVPTQ
LQRLLTHNAGVRVLKACAVIWVGGAVLSPGLMKAARAEGLRLAPCYGATETAAMVTALAPEDFLAGATGCGSPLEDVELQ
LTQAGALQVRTARLALARWLGEGLEDVRDQQGWWTSGDAGCLITESNRVWLSIAGRLDGAIHSGGETVFPEQLEARLQVA
AVGLPVEAVLLLAVEDREWGQRLVALVRPAAGANGEDLIKQLRHLCADWPPADRPMHWRLCPMLAPMANGKWQRGYWQQW
LRLLEAESTEF
>Mature_411_residues
MRELVALRCNPAQAEQRALQLAQALEQGDWVQLLPPLGTSGLIPRALLPDGPGVVVASGGTSGGQQHCLQPCSHLDLSAS
ATGQWLQQQGYDLQQCMVLNPLPLHHVSGLMSWWRSRCWRIGHTWLQPGLMRDPDALEQLCGSLPGWGEQLVLMSLVPTQ
LQRLLTHNAGVRVLKACAVIWVGGAVLSPGLMKAARAEGLRLAPCYGATETAAMVTALAPEDFLAGATGCGSPLEDVELQ
LTQAGALQVRTARLALARWLGEGLEDVRDQQGWWTSGDAGCLITESNRVWLSIAGRLDGAIHSGGETVFPEQLEARLQVA
AVGLPVEAVLLLAVEDREWGQRLVALVRPAAGANGEDLIKQLRHLCADWPPADRPMHWRLCPMLAPMANGKWQRGYWQQW
LRLLEAESTEF

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788595, Length=249, Percent_Identity=34.5381526104418, Blast_Score=96, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17558820, Length=327, Percent_Identity=25.3822629969419, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 44656; Mature: 44656

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS00228 TUBULIN_B_AUTOREG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRELVALRCNPAQAEQRALQLAQALEQGDWVQLLPPLGTSGLIPRALLPDGPGVVVASGG
CCCCEEEECCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHCCCCCCCEEEECCC
TSGGQQHCLQPCSHLDLSASATGQWLQQQGYDLQQCMVLNPLPLHHVSGLMSWWRSRCWR
CCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHH
IGHTWLQPGLMRDPDALEQLCGSLPGWGEQLVLMSLVPTQLQRLLTHNAGVRVLKACAVI
HHHHHHCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
WVGGAVLSPGLMKAARAEGLRLAPCYGATETAAMVTALAPEDFLAGATGCGSPLEDVELQ
HHCHHHHCHHHHHHHHHCCCEEECCCCCCHHHHHHHHCCCHHHHCCCCCCCCCHHHHHEE
LTQAGALQVRTARLALARWLGEGLEDVRDQQGWWTSGDAGCLITESNRVWLSIAGRLDGA
EECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCEEEEEEECCCCCH
IHSGGETVFPEQLEARLQVAAVGLPVEAVLLLAVEDREWGQRLVALVRPAAGANGEDLIK
HCCCCCCCCHHHHHHHHHHHHCCCCHHHEEEEEECCHHHHHHHHHHHCCCCCCCHHHHHH
QLRHLCADWPPADRPMHWRLCPMLAPMANGKWQRGYWQQWLRLLEAESTEF
HHHHHHCCCCCCCCCCCEEHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRELVALRCNPAQAEQRALQLAQALEQGDWVQLLPPLGTSGLIPRALLPDGPGVVVASGG
CCCCEEEECCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHCCCCCCCEEEECCC
TSGGQQHCLQPCSHLDLSASATGQWLQQQGYDLQQCMVLNPLPLHHVSGLMSWWRSRCWR
CCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHH
IGHTWLQPGLMRDPDALEQLCGSLPGWGEQLVLMSLVPTQLQRLLTHNAGVRVLKACAVI
HHHHHHCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
WVGGAVLSPGLMKAARAEGLRLAPCYGATETAAMVTALAPEDFLAGATGCGSPLEDVELQ
HHCHHHHCHHHHHHHHHCCCEEECCCCCCHHHHHHHHCCCHHHHCCCCCCCCCHHHHHEE
LTQAGALQVRTARLALARWLGEGLEDVRDQQGWWTSGDAGCLITESNRVWLSIAGRLDGA
EECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCEEEEEEECCCCCH
IHSGGETVFPEQLEARLQVAAVGLPVEAVLLLAVEDREWGQRLVALVRPAAGANGEDLIK
HCCCCCCCCHHHHHHHHHHHHCCCCHHHEEEEEECCHHHHHHHHHHHCCCCCCCHHHHHH
QLRHLCADWPPADRPMHWRLCPMLAPMANGKWQRGYWQQWLRLLEAESTEF
HHHHHHCCCCCCCCCCCEEHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609; 8206837 [H]