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The map label for this gene is ndhI [H]

Identifier: 33864284

GI number: 33864284

Start: 2132110

End: 2132766

Strand: Reverse

Name: ndhI [H]

Synonym: PMT2019

Alternate gene names: 33864284

Gene position: 2132766-2132110 (Counterclockwise)

Preceding gene: 33864285

Following gene: 33864283

Centisome position: 88.46

GC content: 52.05

Gene sequence:

>657_bases
ATGTTCGGGTTCCTCAAAAAAGTTGTTGACTACACCCGCGACGCTGCGGATGCAGCGAACTATCTGATCCAGGGCCTTGC
GGTCACATTCGATCACCTGCGCCGGCGTCCGATCACGGTGCAGTATCCATACGAGAAGCTCATCCCTTCAGAGCGTTATC
GCGGTCGTATCCACTACGAGTTTGATAAATGCATTGCTTGTGAGGTTTGCGTCAGGGTTTGTCCCATCAACTTGCCTGTT
GTGGACTGGGTGATGAATAAGGAGACGAAGAAAAAGGAGCTGCGCAATTATTCCATTGATTTCGGTGTCTGCATCTTTTG
TGGCAACTGTGTGGAGTACTGCCCCACCAATTGCTTATCGATGACAGAGGAATATGAGCTGGCTGCGTACGATCGCCATA
GCCTCAACTACGACAATGTTGCATTGGGTCGTTTGCCAACCAGTGTGACGACAGACCCATCTGTGCAGCCTTTACGAGAG
CTGGCTTATCTGCCTAAAGGGGTTATGGATCCCCATGATGTCCCTGCAAATCAACCTCGAGCCGGACAGCTCCCGGCAGA
GGCGCTCAAGAGTCTGAGCTTGCAACAAGAGTCTGTGCAAGGGGATGAGGGAGAATCCTTGCAGGATGCTTCTGATCAAG
ACCAGCCCAGCGGATGA

Upstream 100 bases:

>100_bases
ATCTCGCTCCGGCAGGGCTAAGCACTTTTACTTATCCTGGCTAAAAAGCTTGTAGGATTTCTTGAATGCTCCTAGTGGGC
CTCTTAGAGGTTCCTTTCGC

Downstream 100 bases:

>100_bases
CGATTGCTACTTCCACAGAATTAATCTGTTTCCTGGTACTAGGCGCCGTGGTGGTTCTCGGCAGCCTTGGTGTCGTCTTG
CTGGGCAATATCGTCTACTC

Product: NADH dehydrogenase subunit I

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase I subunit I; NDH-1 subunit I; NDH-I [H]

Number of amino acids: Translated: 218; Mature: 218

Protein sequence:

>218_residues
MFGFLKKVVDYTRDAADAANYLIQGLAVTFDHLRRRPITVQYPYEKLIPSERYRGRIHYEFDKCIACEVCVRVCPINLPV
VDWVMNKETKKKELRNYSIDFGVCIFCGNCVEYCPTNCLSMTEEYELAAYDRHSLNYDNVALGRLPTSVTTDPSVQPLRE
LAYLPKGVMDPHDVPANQPRAGQLPAEALKSLSLQQESVQGDEGESLQDASDQDQPSG

Sequences:

>Translated_218_residues
MFGFLKKVVDYTRDAADAANYLIQGLAVTFDHLRRRPITVQYPYEKLIPSERYRGRIHYEFDKCIACEVCVRVCPINLPV
VDWVMNKETKKKELRNYSIDFGVCIFCGNCVEYCPTNCLSMTEEYELAAYDRHSLNYDNVALGRLPTSVTTDPSVQPLRE
LAYLPKGVMDPHDVPANQPRAGQLPAEALKSLSLQQESVQGDEGESLQDASDQDQPSG
>Mature_218_residues
MFGFLKKVVDYTRDAADAANYLIQGLAVTFDHLRRRPITVQYPYEKLIPSERYRGRIHYEFDKCIACEVCVRVCPINLPV
VDWVMNKETKKKELRNYSIDFGVCIFCGNCVEYCPTNCLSMTEEYELAAYDRHSLNYDNVALGRLPTSVTTDPSVQPLRE
LAYLPKGVMDPHDVPANQPRAGQLPAEALKSLSLQQESVQGDEGESLQDASDQDQPSG

Specific function: NDH-1 shuttles electrons from an unknown electron donor, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory and/or the photosynthetic chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone

COG id: COG1143

COG function: function code C; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I)

Gene ontology:

Cell location: Cellular thylakoid membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI4505371, Length=126, Percent_Identity=31.7460317460317, Blast_Score=74, Evalue=9e-14,
Organism=Escherichia coli, GI1788617, Length=110, Percent_Identity=34.5454545454545, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789075, Length=135, Percent_Identity=30.3703703703704, Blast_Score=65, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17555194, Length=126, Percent_Identity=30.1587301587302, Blast_Score=70, Evalue=7e-13,
Organism=Drosophila melanogaster, GI17864306, Length=143, Percent_Identity=30.7692307692308, Blast_Score=76, Evalue=1e-14,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012285
- InterPro:   IPR004497
- InterPro:   IPR010226 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: 1.6.5.3

Molecular weight: Translated: 24632; Mature: 24632

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
4.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGFLKKVVDYTRDAADAANYLIQGLAVTFDHLRRRPITVQYPYEKLIPSERYRGRIHYE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHCCCCHHHCCCEEEH
FDKCIACEVCVRVCPINLPVVDWVMNKETKKKELRNYSIDFGVCIFCGNCVEYCPTNCLS
HHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHCCCCHHHHHHHCCHHHHCCCHHHH
MTEEYELAAYDRHSLNYDNVALGRLPTSVTTDPSVQPLRELAYLPKGVMDPHDVPANQPR
HHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCC
AGQLPAEALKSLSLQQESVQGDEGESLQDASDQDQPSG
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MFGFLKKVVDYTRDAADAANYLIQGLAVTFDHLRRRPITVQYPYEKLIPSERYRGRIHYE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHCCCCHHHCCCEEEH
FDKCIACEVCVRVCPINLPVVDWVMNKETKKKELRNYSIDFGVCIFCGNCVEYCPTNCLS
HHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHCCCCHHHHHHHCCHHHHCCCHHHH
MTEEYELAAYDRHSLNYDNVALGRLPTSVTTDPSVQPLRELAYLPKGVMDPHDVPANQPR
HHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCC
AGQLPAEALKSLSLQQESVQGDEGESLQDASDQDQPSG
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA