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The map label for this gene is ppnK

Identifier: 33864281

GI number: 33864281

Start: 2130182

End: 2131129

Strand: Reverse

Name: ppnK

Synonym: PMT2016

Alternate gene names: 33864281

Gene position: 2131129-2130182 (Counterclockwise)

Preceding gene: 33864282

Following gene: 33864280

Centisome position: 88.4

GC content: 54.75

Gene sequence:

>948_bases
ATGCGTCTCGATTTGGTCTGGGTGATCTATCGGGCGGGAAGTCATTCTGCGCATCGTGAAGCCTGTTGTTGTGTTGACGA
GCTCCAAGCGCTTGGAGTGAAAGTGGTGATGGCCATGAGTGGAGTGACGGCCAACCCTTTCCCCGATTTATTGGCCTCAG
AAGCCGGCCTGCCTGATTTGGCTGTTGTGCTTGGGGGCGATGGCACGGTGCTCGGTGCGGCCCGTCATCTTGCCGTTCAT
GATGTACCAATCCTTAGTTTCAACGTCGGAGGGCATCTAGGTTTTCTGACGCATGAACGCTGTTTGCTTGATGGCGGTCA
GCTATGGCAGCGATTGTTGCAGGACAACTTTGCTTTGGAGCGCAGGATGATGTTGCAGGCTGCCGTGGACAGCCGCTCCC
CTGCTGAACGAACTGCTCGACCTACTGCTTCGCTCCAGGATTTGAATGGGACTAAGGCACTGCATTGGGCCCTGAATGAT
TTTTATATGCGTCCTTATCGCGATGATGTGTCGCCTACCTGCACCCTTGAACTAGAGATCGATGGTGAGGTGGTGGATCA
TTACCGTGGCGATGGTTTGATCTTGGCGACACCCACAGGGTCTACCGGCTACTCGATGGCGTCTGGAGGACCGATTCTTC
ACCCTGGCATTGATGCCATCATCGTGAGTCCAATTTGTCCGATGAGTCTCTCTAGTCGTCCCGTCATCGTGCCTCCAGCT
TCACGGCTCGTGATTGGGCTTCTGGGAGAAAACACTCGCCGGGTGAAGCTCTGGAAAGATGGGGCGAGTGGTGCCCTGCT
TGAACCAGGCCAGTGTTGTGTTGTTCAGAGGGCTCGGCACCATGCATTGATGGTGGTTCTTGAACAGAGCCCCTCTTATT
ACAGAACTCTCACGCACAAGCTGCATTGGGCGGGAAGTCTGCTCAACAATCAACCATCTCCCAGCTGA

Upstream 100 bases:

>100_bases
GGCAGCTGTGGGTCTTGCGATCCTCCTTTCCTTGTATCGCAATCGCGTCACTGTGGATATGGAGCGCTTCAATCTGCTGC
GCTGGTAAGGAGACCCAGGC

Downstream 100 bases:

>100_bases
AGCTCATGGGCCTCGAGATCGAACGGAGGTTTTTGGTGGTGGGAGAAGAGTGGCGAGCTTTAGCAGGACCCGCCTTGCCT
TTGCGTCAGGGTTACCTGGC

Product: inorganic polyphosphate/ATP-NAD kinase

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase 2

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MRLDLVWVIYRAGSHSAHREACCCVDELQALGVKVVMAMSGVTANPFPDLLASEAGLPDLAVVLGGDGTVLGAARHLAVH
DVPILSFNVGGHLGFLTHERCLLDGGQLWQRLLQDNFALERRMMLQAAVDSRSPAERTARPTASLQDLNGTKALHWALND
FYMRPYRDDVSPTCTLELEIDGEVVDHYRGDGLILATPTGSTGYSMASGGPILHPGIDAIIVSPICPMSLSSRPVIVPPA
SRLVIGLLGENTRRVKLWKDGASGALLEPGQCCVVQRARHHALMVVLEQSPSYYRTLTHKLHWAGSLLNNQPSPS

Sequences:

>Translated_315_residues
MRLDLVWVIYRAGSHSAHREACCCVDELQALGVKVVMAMSGVTANPFPDLLASEAGLPDLAVVLGGDGTVLGAARHLAVH
DVPILSFNVGGHLGFLTHERCLLDGGQLWQRLLQDNFALERRMMLQAAVDSRSPAERTARPTASLQDLNGTKALHWALND
FYMRPYRDDVSPTCTLELEIDGEVVDHYRGDGLILATPTGSTGYSMASGGPILHPGIDAIIVSPICPMSLSSRPVIVPPA
SRLVIGLLGENTRRVKLWKDGASGALLEPGQCCVVQRARHHALMVVLEQSPSYYRTLTHKLHWAGSLLNNQPSPS
>Mature_315_residues
MRLDLVWVIYRAGSHSAHREACCCVDELQALGVKVVMAMSGVTANPFPDLLASEAGLPDLAVVLGGDGTVLGAARHLAVH
DVPILSFNVGGHLGFLTHERCLLDGGQLWQRLLQDNFALERRMMLQAAVDSRSPAERTARPTASLQDLNGTKALHWALND
FYMRPYRDDVSPTCTLELEIDGEVVDHYRGDGLILATPTGSTGYSMASGGPILHPGIDAIIVSPICPMSLSSRPVIVPPA
SRLVIGLLGENTRRVKLWKDGASGALLEPGQCCVVQRARHHALMVVLEQSPSYYRTLTHKLHWAGSLLNNQPSPS

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family

Homologues:

Organism=Homo sapiens, GI55743112, Length=256, Percent_Identity=29.6875, Blast_Score=82, Evalue=7e-16,
Organism=Escherichia coli, GI1788968, Length=261, Percent_Identity=33.3333333333333, Blast_Score=129, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6320794, Length=260, Percent_Identity=30, Blast_Score=108, Evalue=8e-25,
Organism=Saccharomyces cerevisiae, GI6322509, Length=263, Percent_Identity=30.0380228136882, Blast_Score=102, Evalue=7e-23,
Organism=Saccharomyces cerevisiae, GI6325068, Length=225, Percent_Identity=34.2222222222222, Blast_Score=100, Evalue=3e-22,
Organism=Drosophila melanogaster, GI28573828, Length=249, Percent_Identity=32.9317269076305, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI28573830, Length=249, Percent_Identity=32.9317269076305, Blast_Score=88, Evalue=6e-18,
Organism=Drosophila melanogaster, GI161077047, Length=249, Percent_Identity=32.9317269076305, Blast_Score=88, Evalue=7e-18,
Organism=Drosophila melanogaster, GI28573832, Length=249, Percent_Identity=32.9317269076305, Blast_Score=88, Evalue=8e-18,
Organism=Drosophila melanogaster, GI28573826, Length=249, Percent_Identity=32.9317269076305, Blast_Score=88, Evalue=8e-18,
Organism=Drosophila melanogaster, GI20129957, Length=283, Percent_Identity=28.9752650176678, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI281363321, Length=279, Percent_Identity=28.673835125448, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24653424, Length=279, Percent_Identity=28.673835125448, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI281363323, Length=279, Percent_Identity=28.673835125448, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24653422, Length=283, Percent_Identity=28.9752650176678, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PPNK2_PROMM (Q7V4D8)

Other databases:

- EMBL:   BX548175
- RefSeq:   NP_895841.1
- ProteinModelPortal:   Q7V4D8
- SMR:   Q7V4D8
- STRING:   Q7V4D8
- GeneID:   1728997
- GenomeReviews:   BX548175_GR
- KEGG:   pmt:PMT2016
- NMPDR:   fig|74547.1.peg.2008
- eggNOG:   COG0061
- HOGENOM:   HBG713904
- OMA:   KLHWAGS
- ProtClustDB:   PRK02645
- BioCyc:   PMAR74547:PMT2016-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00361
- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504
- Gene3D:   G3DSA:2.60.200.30
- Gene3D:   G3DSA:3.40.50.10330
- PANTHER:   PTHR20275

Pfam domain/function: PF01513 NAD_kinase; SSF111331 ATP-NAD_kinase_PpnK-typ

EC number: =2.7.1.23

Molecular weight: Translated: 34060; Mature: 34060

Theoretical pI: Translated: 6.83; Mature: 6.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLDLVWVIYRAGSHSAHREACCCVDELQALGVKVVMAMSGVTANPFPDLLASEAGLPDL
CCEEEEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHCCCCCCE
AVVLGGDGTVLGAARHLAVHDVPILSFNVGGHLGFLTHERCLLDGGQLWQRLLQDNFALE
EEEECCCCEEEHHEHEEEEECCEEEEEECCCEEEEEECCHHHCCCHHHHHHHHHCCHHHH
RRMMLQAAVDSRSPAERTARPTASLQDLNGTKALHWALNDFYMRPYRDDVSPTCTLELEI
HHHHHHHHHCCCCCHHHHCCCCCCHHCCCCCEEEEEEHHHHCCCCCCCCCCCEEEEEEEE
DGEVVDHYRGDGLILATPTGSTGYSMASGGPILHPGIDAIIVSPICPMSLSSRPVIVPPA
CCCEEEEECCCEEEEECCCCCCCCCCCCCCCEECCCCCEEEECCCCCCCCCCCCEEECCH
SRLVIGLLGENTRRVKLWKDGASGALLEPGQCCVVQRARHHALMVVLEQSPSYYRTLTHK
HHEEEEEECCCCEEEEEEECCCCCCCCCCCCHHHHHHHCCEEEEEEEECCCCHHHHHHHH
LHWAGSLLNNQPSPS
HHHHHHHHCCCCCCC
>Mature Secondary Structure
MRLDLVWVIYRAGSHSAHREACCCVDELQALGVKVVMAMSGVTANPFPDLLASEAGLPDL
CCEEEEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHCCCCCCE
AVVLGGDGTVLGAARHLAVHDVPILSFNVGGHLGFLTHERCLLDGGQLWQRLLQDNFALE
EEEECCCCEEEHHEHEEEEECCEEEEEECCCEEEEEECCHHHCCCHHHHHHHHHCCHHHH
RRMMLQAAVDSRSPAERTARPTASLQDLNGTKALHWALNDFYMRPYRDDVSPTCTLELEI
HHHHHHHHHCCCCCHHHHCCCCCCHHCCCCCEEEEEEHHHHCCCCCCCCCCCEEEEEEEE
DGEVVDHYRGDGLILATPTGSTGYSMASGGPILHPGIDAIIVSPICPMSLSSRPVIVPPA
CCCEEEEECCCEEEEECCCCCCCCCCCCCCCEECCCCCEEEECCCCCCCCCCCCEEECCH
SRLVIGLLGENTRRVKLWKDGASGALLEPGQCCVVQRARHHALMVVLEQSPSYYRTLTHK
HHEEEEEECCCCEEEEEEECCCCCCCCCCCCHHHHHHHCCEEEEEEEECCCCHHHHHHHH
LHWAGSLLNNQPSPS
HHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917642