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The map label for this gene is cca [H]

Identifier: 33864176

GI number: 33864176

Start: 2016021

End: 2017280

Strand: Reverse

Name: cca [H]

Synonym: PMT1910

Alternate gene names: 33864176

Gene position: 2017280-2016021 (Counterclockwise)

Preceding gene: 33864180

Following gene: 33864172

Centisome position: 83.67

GC content: 57.62

Gene sequence:

>1260_bases
ATGGAGAGTCTGGTGAAGCCAGAGCCTGGATTCGATGGACCAGGTTTGCCAATGGGTTTGTTGGCGGTACTGCAGCAAGT
TGCGTCAGAGGTTGGGATTAAACGACTTGCCTTGGTGGGTGGTGCGGTTCGAGATGGCCTGTTGCATCGGGTTCATTTTG
ATCCCTGGCGAGGTTTGCCTGATCTTGATCTGGTGGTGGAGGGTTCAGCGGTGGTTTTAGCTAAGGCGCTTCGCCGTCAT
TTAGGGCCAGAGCAGTTGCCGCAGCTGCGTGTGCATGGGGCTTACGGAACGGTGGAACTTGTGGTTGATGGGGTCTTGCT
TGATTTAGCCACTGCTCGGCAAGAAACCTATGCATCTCCTGGACAAAACCCGCAGGTCACGGAGGGTCATCTCGAAGAAG
ACTTAGCCCGAAGGGACTTCACGGTGAATGCCATGGCTTTGGAGTTGCCAGGGATGACCTTGTTGGATCCCCATGATGGC
TGGGCGGCTTTAGCCATGCGACAGCTGGTTTTTTTACATTCAAACAGTGTCGCGGATGATCCGACGCGAGTCGTGCGAGG
GGCCCGTTATGCGGCCCGGCTTGGTTTTGTTTTGGCGCCTGAGGCTCTTGCTCAGGTGCGCTCCACTCTTCAATCCTGGC
CTTGGGCGTGGCATCCTGGCGAAGCGCCGAAGCTGGCACCGCCAGCTCTTTCCACGCGCTTGCGGATGGAGCTGGAGTTG
TTATTGGAAAGGGAGCCTTGGCAGAAGGCAGTGGCTCATTTGCAGGACTGGGGAGCTCTGGTGTTACTTGATGAGGGCCT
TCAGGCTGATCAAAACTGGCATCGTCGAATGCGCTGGGCCTGGCGTTTGGAGCTGCCGTTGCTGACAGCATTGGTGGCCG
GAGCTGCGGATCCTCTGGCTATGGCAGAACGCTTGCAGTTGCCACAATTGCAGCAAAGGTTGTTGGCTGAAGCGGCCGAA
TTGCAGATTTTGTTGGCTTCGCTAGAGGTCGCTGAGAGTTTGTCGACGTGGTCACCCGCTCGCTGGTGCGAAACCCTGGA
GGGCAATGGTTGGCAGCCAGAGGCGGTTGCTCTTTGCGTTTGCTTGGGTGTACCGATGTGGCGACCCTTGCTGCGTTGGT
GGGGGCGTTGGCGGCAGGTGAAGTCTCCTGTATCGGCACAGGCTTTGATTGATCAGGGCTGGCGACCAGGTCCGGCTTTG
GGGGCCGAATTACAGCGGCTCAGGCTGGAGCTGATCGATCAGCAGGTGGCGTTGAGGTGA

Upstream 100 bases:

>100_bases
AGCGACGGCGATGGCGGCAGCCTCTTCCTGCAGGCAGCGGGTGAGGGCAGACAAACTGTAAGTTCGTGATGATGCGGTAA
TTCTCCTGCATACCACCGCC

Downstream 100 bases:

>100_bases
TGTTGTTCTGGTGGCGCTTTGGGCTGGGAGTAGTGGTCCTGACCAATCAATGCGTTGTCGGCTTAAGCCCCCCTGTTCAA
GAGAGCTGAACAAGGTGGGT

Product: tRNA nucleotidyltransferase/poly(A) polymerase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MESLVKPEPGFDGPGLPMGLLAVLQQVASEVGIKRLALVGGAVRDGLLHRVHFDPWRGLPDLDLVVEGSAVVLAKALRRH
LGPEQLPQLRVHGAYGTVELVVDGVLLDLATARQETYASPGQNPQVTEGHLEEDLARRDFTVNAMALELPGMTLLDPHDG
WAALAMRQLVFLHSNSVADDPTRVVRGARYAARLGFVLAPEALAQVRSTLQSWPWAWHPGEAPKLAPPALSTRLRMELEL
LLEREPWQKAVAHLQDWGALVLLDEGLQADQNWHRRMRWAWRLELPLLTALVAGAADPLAMAERLQLPQLQQRLLAEAAE
LQILLASLEVAESLSTWSPARWCETLEGNGWQPEAVALCVCLGVPMWRPLLRWWGRWRQVKSPVSAQALIDQGWRPGPAL
GAELQRLRLELIDQQVALR

Sequences:

>Translated_419_residues
MESLVKPEPGFDGPGLPMGLLAVLQQVASEVGIKRLALVGGAVRDGLLHRVHFDPWRGLPDLDLVVEGSAVVLAKALRRH
LGPEQLPQLRVHGAYGTVELVVDGVLLDLATARQETYASPGQNPQVTEGHLEEDLARRDFTVNAMALELPGMTLLDPHDG
WAALAMRQLVFLHSNSVADDPTRVVRGARYAARLGFVLAPEALAQVRSTLQSWPWAWHPGEAPKLAPPALSTRLRMELEL
LLEREPWQKAVAHLQDWGALVLLDEGLQADQNWHRRMRWAWRLELPLLTALVAGAADPLAMAERLQLPQLQQRLLAEAAE
LQILLASLEVAESLSTWSPARWCETLEGNGWQPEAVALCVCLGVPMWRPLLRWWGRWRQVKSPVSAQALIDQGWRPGPAL
GAELQRLRLELIDQQVALR
>Mature_419_residues
MESLVKPEPGFDGPGLPMGLLAVLQQVASEVGIKRLALVGGAVRDGLLHRVHFDPWRGLPDLDLVVEGSAVVLAKALRRH
LGPEQLPQLRVHGAYGTVELVVDGVLLDLATARQETYASPGQNPQVTEGHLEEDLARRDFTVNAMALELPGMTLLDPHDG
WAALAMRQLVFLHSNSVADDPTRVVRGARYAARLGFVLAPEALAQVRSTLQSWPWAWHPGEAPKLAPPALSTRLRMELEL
LLEREPWQKAVAHLQDWGALVLLDEGLQADQNWHRRMRWAWRLELPLLTALVAGAADPLAMAERLQLPQLQQRLLAEAAE
LQILLASLEVAESLSTWSPARWCETLEGNGWQPEAVALCVCLGVPMWRPLLRWWGRWRQVKSPVSAQALIDQGWRPGPAL
GAELQRLRLELIDQQVALR

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789436, Length=263, Percent_Identity=34.6007604562738, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 46464; Mature: 46464

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESLVKPEPGFDGPGLPMGLLAVLQQVASEVGIKRLALVGGAVRDGLLHRVHFDPWRGLP
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
DLDLVVEGSAVVLAKALRRHLGPEQLPQLRVHGAYGTVELVVDGVLLDLATARQETYASP
CCCEEECCCHHHHHHHHHHHCCHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCC
GQNPQVTEGHLEEDLARRDFTVNAMALELPGMTLLDPHDGWAALAMRQLVFLHSNSVADD
CCCCCCCCCHHHHHHHHCCCEEEEEEEECCCCEEECCCCCHHHHHHHHHHHHHCCCCCCC
PTRVVRGARYAARLGFVLAPEALAQVRSTLQSWPWAWHPGEAPKLAPPALSTRLRMELEL
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHH
LLEREPWQKAVAHLQDWGALVLLDEGLQADQNWHRRMRWAWRLELPLLTALVAGAADPLA
HHCCCHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHEECHHHHHHHHHCCCCHHH
MAERLQLPQLQQRLLAEAAELQILLASLEVAESLSTWSPARWCETLEGNGWQPEAVALCV
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHH
CLGVPMWRPLLRWWGRWRQVKSPVSAQALIDQGWRPGPALGAELQRLRLELIDQQVALR
HHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MESLVKPEPGFDGPGLPMGLLAVLQQVASEVGIKRLALVGGAVRDGLLHRVHFDPWRGLP
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
DLDLVVEGSAVVLAKALRRHLGPEQLPQLRVHGAYGTVELVVDGVLLDLATARQETYASP
CCCEEECCCHHHHHHHHHHHCCHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCC
GQNPQVTEGHLEEDLARRDFTVNAMALELPGMTLLDPHDGWAALAMRQLVFLHSNSVADD
CCCCCCCCCHHHHHHHHCCCEEEEEEEECCCCEEECCCCCHHHHHHHHHHHHHCCCCCCC
PTRVVRGARYAARLGFVLAPEALAQVRSTLQSWPWAWHPGEAPKLAPPALSTRLRMELEL
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHH
LLEREPWQKAVAHLQDWGALVLLDEGLQADQNWHRRMRWAWRLELPLLTALVAGAADPLA
HHCCCHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHEECHHHHHHHHHCCCCHHH
MAERLQLPQLQQRLLAEAAELQILLASLEVAESLSTWSPARWCETLEGNGWQPEAVALCV
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHH
CLGVPMWRPLLRWWGRWRQVKSPVSAQALIDQGWRPGPALGAELQRLRLELIDQQVALR
HHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA