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The map label for this gene is 33864145

Identifier: 33864145

GI number: 33864145

Start: 1984161

End: 1985684

Strand: Reverse

Name: 33864145

Synonym: PMT1879

Alternate gene names: NA

Gene position: 1985684-1984161 (Counterclockwise)

Preceding gene: 33864146

Following gene: 33864141

Centisome position: 82.36

GC content: 52.69

Gene sequence:

>1524_bases
TTGATCAGCCTTGCAGTGAGCACAGTGAGCGATAGCCCTTCAACAGTGTCCGCGACCTCAATAGATGTACCCGGCGGGTT
TGATCGGGATGACTGGGCAAGTGGTTATTGCAATGTTGAGCAAGAGCTCAATGCTGTGTCTTTACGTCCCCTGAGGGGAC
AGATTCCAGCTGAACTGCAAGGCAGCCACTATCGCAATGGTCCAGGCAGGTTTGAACGCGATGGGCAATGGGTGCATCAC
CCCTTTGATGGTGACGGCATGATCACTGCTGTGCGATTCAGCGATAGCAAGGCCACGCTGAGTAATCGCTTTGTGCGAAC
GAAATATTGGCAGGAAGAGGAAAAGGCCGGTCGCTTCCTCTATCGGGGGGTGTTTGGCACGCAGAAGCGTGGTGGGGTGA
AGGCGAATGCTTTTGACGTGCGTTTCAAGAACATTGCCAATACCCAAGTGGTTCAACTTGGCGATCAGTTACTGGCTCTT
TGGGAGGCGGCTGGCCCGCACGCGCTTGACCCTGTCAGCCTGGAAACTCATGGGCTTTCGAGTCTCAATGGGGTGCTGAA
GCCTGGAGAGGCTTTTAGCGCTCATCCCCGCTTCGATCCTGGCCACCATGGCGATCCTCGTATGGTCACCTTTGGTGTGA
ACACAGGGCCGACTAGCAAGATCCGGCTGATGGAGTTTGCCACTCAGGGCCCAGATGCGGGACGCCTCATCAGTGATCGC
ACAGACAGTTTTAAGGGTTTTGCTTTTCTGCATGACTTCGCGATTACTCCCAACTGGACGCTTTTTTTGCAGAATGCGAT
CAACTTCAATCCGTTGCCGTTTGTGCTGGGTCAGAAGGGTGCGGCGCAATGTTTAACGTCTAAGCCTGGAGGCCAGGCAA
AGTTTTGGTTGATTCCTCGTGATTGCGGCACTTTTGCTGGGCAGCCGCCGCGCATCTTTGATGCTCCCGATGGTTTTGTT
TTTCACCATCTCAATGCTTGGGAGGAGGGGGATGAGTTGGTGTTGGAGAGCATTTACTACAGCGATTACCCCTCGATTGA
TCCCGGCGAGGACTTCCTCTCGATGGACTTTGACTTTTTTCCTGAGGGTCGATTAGAGCAGTGCCGCATCAATCTTTTGG
GGAAAGGAATTGAAACCAAGCGCTTTAGTGAGCGTTGTTGTGAATTTGCGATGGTCAATCCACGCCGAGAAGGGTTGTCG
GCTCGTTATGCATGGATGGCTGTGGCAGAGCGTGAAACCGGCTATGCCCCCCTACAGGCGATCAAAAAACTGGATTTGAG
CAATGGTGATCAATGCTTATGGAGTGCAGCGCCGCGTGGATTTGTCAGTGAGCCATTGATGGTGCCACGCCCTGGCAGTG
AAGCTGAGGATGATGGTTGGCTGTTGGTTTTTGTATGGAATGGAGAGCGTCGTGCCAGTCAGTTGTTGATTTTGAATGCA
AACGATTTAAGCGAGCAGGCAGTGCTTGAGATGCCTATAACCATTCCATACGGATTACATGGCAGTTGGGTTGCTGCTGA
TTGA

Upstream 100 bases:

>100_bases
TGTGCTGGAGCAGGCTGGCGGGCAGAAGAGCTGAATGCTTGGGGAGTGGGGATTTCCTCGCTCGCGGCTGTAAGCCCTTT
TGCGTAAGGATTAGTCAATC

Downstream 100 bases:

>100_bases
TTGATTGAGTTTAGGGTGTTGATTGGCGAACAAATTCCAAGCTTATTTGGAATTTGTTCGCTCTGAGCTTCAGGATTTGG
TTGGTTGACTAGATAAAGTC

Product: retinal pigment epithelial membrane protein

Products: NA

Alternate protein names: ACO; 8'-apo-beta-carotenal 15,15'-oxygenase; Diox1 [H]

Number of amino acids: Translated: 507; Mature: 507

Protein sequence:

>507_residues
MISLAVSTVSDSPSTVSATSIDVPGGFDRDDWASGYCNVEQELNAVSLRPLRGQIPAELQGSHYRNGPGRFERDGQWVHH
PFDGDGMITAVRFSDSKATLSNRFVRTKYWQEEEKAGRFLYRGVFGTQKRGGVKANAFDVRFKNIANTQVVQLGDQLLAL
WEAAGPHALDPVSLETHGLSSLNGVLKPGEAFSAHPRFDPGHHGDPRMVTFGVNTGPTSKIRLMEFATQGPDAGRLISDR
TDSFKGFAFLHDFAITPNWTLFLQNAINFNPLPFVLGQKGAAQCLTSKPGGQAKFWLIPRDCGTFAGQPPRIFDAPDGFV
FHHLNAWEEGDELVLESIYYSDYPSIDPGEDFLSMDFDFFPEGRLEQCRINLLGKGIETKRFSERCCEFAMVNPRREGLS
ARYAWMAVAERETGYAPLQAIKKLDLSNGDQCLWSAAPRGFVSEPLMVPRPGSEAEDDGWLLVFVWNGERRASQLLILNA
NDLSEQAVLEMPITIPYGLHGSWVAAD

Sequences:

>Translated_507_residues
MISLAVSTVSDSPSTVSATSIDVPGGFDRDDWASGYCNVEQELNAVSLRPLRGQIPAELQGSHYRNGPGRFERDGQWVHH
PFDGDGMITAVRFSDSKATLSNRFVRTKYWQEEEKAGRFLYRGVFGTQKRGGVKANAFDVRFKNIANTQVVQLGDQLLAL
WEAAGPHALDPVSLETHGLSSLNGVLKPGEAFSAHPRFDPGHHGDPRMVTFGVNTGPTSKIRLMEFATQGPDAGRLISDR
TDSFKGFAFLHDFAITPNWTLFLQNAINFNPLPFVLGQKGAAQCLTSKPGGQAKFWLIPRDCGTFAGQPPRIFDAPDGFV
FHHLNAWEEGDELVLESIYYSDYPSIDPGEDFLSMDFDFFPEGRLEQCRINLLGKGIETKRFSERCCEFAMVNPRREGLS
ARYAWMAVAERETGYAPLQAIKKLDLSNGDQCLWSAAPRGFVSEPLMVPRPGSEAEDDGWLLVFVWNGERRASQLLILNA
NDLSEQAVLEMPITIPYGLHGSWVAAD
>Mature_507_residues
MISLAVSTVSDSPSTVSATSIDVPGGFDRDDWASGYCNVEQELNAVSLRPLRGQIPAELQGSHYRNGPGRFERDGQWVHH
PFDGDGMITAVRFSDSKATLSNRFVRTKYWQEEEKAGRFLYRGVFGTQKRGGVKANAFDVRFKNIANTQVVQLGDQLLAL
WEAAGPHALDPVSLETHGLSSLNGVLKPGEAFSAHPRFDPGHHGDPRMVTFGVNTGPTSKIRLMEFATQGPDAGRLISDR
TDSFKGFAFLHDFAITPNWTLFLQNAINFNPLPFVLGQKGAAQCLTSKPGGQAKFWLIPRDCGTFAGQPPRIFDAPDGFV
FHHLNAWEEGDELVLESIYYSDYPSIDPGEDFLSMDFDFFPEGRLEQCRINLLGKGIETKRFSERCCEFAMVNPRREGLS
ARYAWMAVAERETGYAPLQAIKKLDLSNGDQCLWSAAPRGFVSEPLMVPRPGSEAEDDGWLLVFVWNGERRASQLLILNA
NDLSEQAVLEMPITIPYGLHGSWVAAD

Specific function: Cleaves a number of carotenals and carotenols in the all-trans configuration at the 15-15' double bond producing retinal or retinol, respectively. Also shows activity toward lycopenals and the corresponding alcohols. Does not cleave beta- carotene or lyco

COG id: COG3670

COG function: function code Q; Lignostilbene-alpha,beta-dioxygenase and related enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carotenoid oxygenase family [H]

Homologues:

Organism=Caenorhabditis elegans, GI32564756, Length=529, Percent_Identity=24.1965973534972, Blast_Score=104, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004294 [H]

Pfam domain/function: PF03055 RPE65 [H]

EC number: =1.14.99.41 [H]

Molecular weight: Translated: 56169; Mature: 56169

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISLAVSTVSDSPSTVSATSIDVPGGFDRDDWASGYCNVEQELNAVSLRPLRGQIPAELQ
CEEEEEEECCCCCCEEEEEEEECCCCCCCCCCCCCCCCHHHCCCEEEECCCCCCCCCCCC
GSHYRNGPGRFERDGQWVHHPFDGDGMITAVRFSDSKATLSNRFVRTKYWQEEEKAGRFL
CCCCCCCCCCCCCCCCEEECCCCCCCEEEEEEECCCHHHHHHCEEEHHHCCCHHHHCCEE
YRGVFGTQKRGGVKANAFDVRFKNIANTQVVQLGDQLLALWEAAGPHALDPVSLETHGLS
EECCCCCCCCCCEECCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCH
SLNGVLKPGEAFSAHPRFDPGHHGDPRMVTFGVNTGPTSKIRLMEFATQGPDAGRLISDR
HHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEEEECCCCCCCCCEECCC
TDSFKGFAFLHDFAITPNWTLFLQNAINFNPLPFVLGQKGAAQCLTSKPGGQAKFWLIPR
CCCCCCEEEEEEEECCCCEEEEEECCCCCCCCCEEECCCCHHHHHCCCCCCCEEEEEEEC
DCGTFAGQPPRIFDAPDGFVFHHLNAWEEGDELVLESIYYSDYPSIDPGEDFLSMDFDFF
CCCCCCCCCCEEEECCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCHHEECCCCCC
PEGRLEQCRINLLGKGIETKRFSERCCEFAMVNPRREGLSARYAWMAVAERETGYAPLQA
CCCCHHHEEEEECCCCCCHHHHHHHHHHHEECCCHHHCCCCEEEEEEEECCCCCCHHHHH
IKKLDLSNGDQCLWSAAPRGFVSEPLMVPRPGSEAEDDGWLLVFVWNGERRASQLLILNA
HHHCCCCCCCEEEEECCCCCCCCCCEECCCCCCCCCCCCEEEEEEECCCCCCEEEEEEEC
NDLSEQAVLEMPITIPYGLHGSWVAAD
CCCCCCEEEEECEEEECCCCCCEEECC
>Mature Secondary Structure
MISLAVSTVSDSPSTVSATSIDVPGGFDRDDWASGYCNVEQELNAVSLRPLRGQIPAELQ
CEEEEEEECCCCCCEEEEEEEECCCCCCCCCCCCCCCCHHHCCCEEEECCCCCCCCCCCC
GSHYRNGPGRFERDGQWVHHPFDGDGMITAVRFSDSKATLSNRFVRTKYWQEEEKAGRFL
CCCCCCCCCCCCCCCCEEECCCCCCCEEEEEEECCCHHHHHHCEEEHHHCCCHHHHCCEE
YRGVFGTQKRGGVKANAFDVRFKNIANTQVVQLGDQLLALWEAAGPHALDPVSLETHGLS
EECCCCCCCCCCEECCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCH
SLNGVLKPGEAFSAHPRFDPGHHGDPRMVTFGVNTGPTSKIRLMEFATQGPDAGRLISDR
HHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEEEECCCCCCCCCEECCC
TDSFKGFAFLHDFAITPNWTLFLQNAINFNPLPFVLGQKGAAQCLTSKPGGQAKFWLIPR
CCCCCCEEEEEEEECCCCEEEEEECCCCCCCCCEEECCCCHHHHHCCCCCCCEEEEEEEC
DCGTFAGQPPRIFDAPDGFVFHHLNAWEEGDELVLESIYYSDYPSIDPGEDFLSMDFDFF
CCCCCCCCCCEEEECCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCHHEECCCCCC
PEGRLEQCRINLLGKGIETKRFSERCCEFAMVNPRREGLSARYAWMAVAERETGYAPLQA
CCCCHHHEEEEECCCCCCHHHHHHHHHHHEECCCHHHCCCCEEEEEEEECCCCCCHHHHH
IKKLDLSNGDQCLWSAAPRGFVSEPLMVPRPGSEAEDDGWLLVFVWNGERRASQLLILNA
HHHCCCCCCCEEEEECCCCCCCCCCEECCCCCCCCCCCCEEEEEEECCCCCCEEEEEEEC
NDLSEQAVLEMPITIPYGLHGSWVAAD
CCCCCCEEEEECEEEECCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]