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The map label for this gene is glyA

Identifier: 33864114

GI number: 33864114

Start: 1955275

End: 1956564

Strand: Reverse

Name: glyA

Synonym: PMT1847

Alternate gene names: 33864114

Gene position: 1956564-1955275 (Counterclockwise)

Preceding gene: 33864118

Following gene: 33864113

Centisome position: 81.16

GC content: 55.58

Gene sequence:

>1290_bases
ATGACGGATCGCTTCTTGGCTTCTATTAATGCTGCCCTGACGGATTCCGACCCGGCGATTGCAGGCTTGATCGATCAGGA
AAGGCAACGTCAGGAGACCCATCTAGAGCTGATAGCCAGTGAGAACTTCACTTCTCAAGCTGTGATGCAGGCTCAGGGTT
CTGTTCTCACCAACAAGTACGCCGAAGGTCTTCCCCATAAGCGCTATTACGGCGGTTGTGAGCATGTCGATGCGATTGAG
GAGTTGGCGATCGAGCGTGCTCAGCGGCTCTTTGGTGCGGCTTGGGCCAACGTGCAACCTCATAGTGGCGCGCAGGCCAA
CTTCGCCGTCTTCCTGGCGCTGTTGCAGCCCGGTGACACCATCATGGGGATGGATCTATCCCATGGGGGGCATCTCACTC
ATGGTTCTCCTGTCAATGTCAGCGGCAAGTGGTTCAAGGTCGTTCATTACGGCGTGGAGCGTGATAGTCAGCAGCTCGAC
ATGGAGGCTGTTCGTCAGCTGGCCCTGAAAGAGCGTCCTCAACTGATCATCTGCGGCTACTCGGCCTATCCCCGCACGAT
CGATTTTGCTGCTTTTCGCAGCATCGCTGATGAGGTGGGCGCTTACTTGTTGGCGGACATGGCACACATTGCCGGCCTTG
TTGCGGCTGGGGTCCATCCGAGTCCTATCGCCCACTGTGATGTGGTGACGACGACCACCCACAAGACATTGCGTGGCCCC
AGAGGCGGCTTAATCCTTTGTCGTGATGCGGACTTTGGCCGTAAGTTTGACAAGGCAGTGTTTCCTGGCAGTCAGGGCGG
CCCTTTGGAACATGTGATTGCAGCCAAGGCTGTGGCCTTAGGAGAAGCTCTTCAGCCAGAGTTCCAGGTTTATAGCTGTC
AGGTGGTGGCGAATGCCCAGGTTCTTGCTGGTCGCATTCAGGAGCGCGGGATTGCGGTGGTGAGTGGAGGAACAGATAAT
CACCTTGTCTTGCTGGATTTGCGCAGCATTGGGATGACCGGGAAGGTTGCTGATTTGCTTGTCAGTGAAGTGAACATCAC
CGCCAATAAGAACACAGTGCCCTTTGATCCTGAGTCGCCTTTTGTGACCAGTGGTCTGCGCTTGGGCACGGCGGCCCTCA
CAACTCGTGGCTTTGATGATGAGGCATTCCGTGAGGTTGCTGATGTGATTGCAGATCGTTTGCTTAAACCGCAAGATGAA
TCGATCAAGGCGCAGTGCTTGGAACGGGTGAGACAACTTTGTGGCCGTTTCCCTCTCTACAGGGATGTCCTCCAGCCAGC
GCTTGCTTGA

Upstream 100 bases:

>100_bases
TTTTTTTGCACTTGGGTCCGGCGGCAGTCTTAGGGCTAAGGGCTTTGCTTAAGACGTGAAGAACCATTGCCTACGATCCG
AAAACTGTTGGGTTCTGTAA

Downstream 100 bases:

>100_bases
CTGGCCCTTCGCTCTGGAATCACAGGTTTGCTCTGCCTAGGATTCATTCCATCGGCCCTTCTTAAGGTCGCTTGCTACAG
GAGCAAACTTGACTCTCGCG

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MTDRFLASINAALTDSDPAIAGLIDQERQRQETHLELIASENFTSQAVMQAQGSVLTNKYAEGLPHKRYYGGCEHVDAIE
ELAIERAQRLFGAAWANVQPHSGAQANFAVFLALLQPGDTIMGMDLSHGGHLTHGSPVNVSGKWFKVVHYGVERDSQQLD
MEAVRQLALKERPQLIICGYSAYPRTIDFAAFRSIADEVGAYLLADMAHIAGLVAAGVHPSPIAHCDVVTTTTHKTLRGP
RGGLILCRDADFGRKFDKAVFPGSQGGPLEHVIAAKAVALGEALQPEFQVYSCQVVANAQVLAGRIQERGIAVVSGGTDN
HLVLLDLRSIGMTGKVADLLVSEVNITANKNTVPFDPESPFVTSGLRLGTAALTTRGFDDEAFREVADVIADRLLKPQDE
SIKAQCLERVRQLCGRFPLYRDVLQPALA

Sequences:

>Translated_429_residues
MTDRFLASINAALTDSDPAIAGLIDQERQRQETHLELIASENFTSQAVMQAQGSVLTNKYAEGLPHKRYYGGCEHVDAIE
ELAIERAQRLFGAAWANVQPHSGAQANFAVFLALLQPGDTIMGMDLSHGGHLTHGSPVNVSGKWFKVVHYGVERDSQQLD
MEAVRQLALKERPQLIICGYSAYPRTIDFAAFRSIADEVGAYLLADMAHIAGLVAAGVHPSPIAHCDVVTTTTHKTLRGP
RGGLILCRDADFGRKFDKAVFPGSQGGPLEHVIAAKAVALGEALQPEFQVYSCQVVANAQVLAGRIQERGIAVVSGGTDN
HLVLLDLRSIGMTGKVADLLVSEVNITANKNTVPFDPESPFVTSGLRLGTAALTTRGFDDEAFREVADVIADRLLKPQDE
SIKAQCLERVRQLCGRFPLYRDVLQPALA
>Mature_428_residues
TDRFLASINAALTDSDPAIAGLIDQERQRQETHLELIASENFTSQAVMQAQGSVLTNKYAEGLPHKRYYGGCEHVDAIEE
LAIERAQRLFGAAWANVQPHSGAQANFAVFLALLQPGDTIMGMDLSHGGHLTHGSPVNVSGKWFKVVHYGVERDSQQLDM
EAVRQLALKERPQLIICGYSAYPRTIDFAAFRSIADEVGAYLLADMAHIAGLVAAGVHPSPIAHCDVVTTTTHKTLRGPR
GGLILCRDADFGRKFDKAVFPGSQGGPLEHVIAAKAVALGEALQPEFQVYSCQVVANAQVLAGRIQERGIAVVSGGTDNH
LVLLDLRSIGMTGKVADLLVSEVNITANKNTVPFDPESPFVTSGLRLGTAALTTRGFDDEAFREVADVIADRLLKPQDES
IKAQCLERVRQLCGRFPLYRDVLQPALA

Specific function: Interconversion of serine and glycine

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family

Homologues:

Organism=Homo sapiens, GI19923315, Length=402, Percent_Identity=51.4925373134328, Blast_Score=364, Evalue=1e-100,
Organism=Homo sapiens, GI261862352, Length=402, Percent_Identity=51.4925373134328, Blast_Score=363, Evalue=1e-100,
Organism=Homo sapiens, GI261862350, Length=402, Percent_Identity=51.4925373134328, Blast_Score=363, Evalue=1e-100,
Organism=Homo sapiens, GI261862348, Length=402, Percent_Identity=51.4925373134328, Blast_Score=363, Evalue=1e-100,
Organism=Homo sapiens, GI261862346, Length=397, Percent_Identity=52.3929471032746, Blast_Score=359, Evalue=3e-99,
Organism=Homo sapiens, GI22547186, Length=411, Percent_Identity=48.4184914841849, Blast_Score=335, Evalue=5e-92,
Organism=Homo sapiens, GI22547189, Length=397, Percent_Identity=47.103274559194, Blast_Score=306, Evalue=2e-83,
Organism=Escherichia coli, GI1788902, Length=410, Percent_Identity=57.0731707317073, Blast_Score=468, Evalue=1e-133,
Organism=Caenorhabditis elegans, GI25144732, Length=396, Percent_Identity=48.2323232323232, Blast_Score=353, Evalue=1e-97,
Organism=Caenorhabditis elegans, GI25144729, Length=396, Percent_Identity=48.2323232323232, Blast_Score=352, Evalue=2e-97,
Organism=Saccharomyces cerevisiae, GI6319739, Length=437, Percent_Identity=43.4782608695652, Blast_Score=357, Evalue=2e-99,
Organism=Saccharomyces cerevisiae, GI6323087, Length=413, Percent_Identity=41.8886198547215, Blast_Score=329, Evalue=4e-91,
Organism=Drosophila melanogaster, GI24640005, Length=412, Percent_Identity=46.8446601941748, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI221329721, Length=412, Percent_Identity=46.8446601941748, Blast_Score=360, Evalue=1e-100,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): GLYA_PROMM (Q7V4U3)

Other databases:

- EMBL:   BX548175
- RefSeq:   NP_895674.1
- ProteinModelPortal:   Q7V4U3
- SMR:   Q7V4U3
- STRING:   Q7V4U3
- GeneID:   1728349
- GenomeReviews:   BX548175_GR
- KEGG:   pmt:PMT1847
- NMPDR:   fig|74547.1.peg.1841
- eggNOG:   COG0112
- HOGENOM:   HBG301263
- OMA:   ISTTTHK
- ProtClustDB:   PRK00011
- BioCyc:   PMAR74547:PMT1847-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00051_B
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11680
- PIRSF:   PIRSF000412

Pfam domain/function: PF00464 SHMT; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.1.2.1

Molecular weight: Translated: 46384; Mature: 46253

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDRFLASINAALTDSDPAIAGLIDQERQRQETHLELIASENFTSQAVMQAQGSVLTNKY
CCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHH
AEGLPHKRYYGGCEHVDAIEELAIERAQRLFGAAWANVQPHSGAQANFAVFLALLQPGDT
HHCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHEEEEECCCCE
IMGMDLSHGGHLTHGSPVNVSGKWFKVVHYGVERDSQQLDMEAVRQLALKERPQLIICGY
EEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHCCHHHHHHHHHHCCCCEEEECC
SAYPRTIDFAAFRSIADEVGAYLLADMAHIAGLVAAGVHPSPIAHCDVVTTTTHKTLRGP
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCHHHHCCC
RGGLILCRDADFGRKFDKAVFPGSQGGPLEHVIAAKAVALGEALQPEFQVYSCQVVANAQ
CCCEEEEECCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEEECHH
VLAGRIQERGIAVVSGGTDNHLVLLDLRSIGMTGKVADLLVSEVNITANKNTVPFDPESP
HHHHHHHHCCEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHEEEEECCCCCCCCCCCC
FVTSGLRLGTAALTTRGFDDEAFREVADVIADRLLKPQDESIKAQCLERVRQLCGRFPLY
CHHCCCHHCHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHH
RDVLQPALA
HHHHHHHCC
>Mature Secondary Structure 
TDRFLASINAALTDSDPAIAGLIDQERQRQETHLELIASENFTSQAVMQAQGSVLTNKY
CCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHH
AEGLPHKRYYGGCEHVDAIEELAIERAQRLFGAAWANVQPHSGAQANFAVFLALLQPGDT
HHCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHEEEEECCCCE
IMGMDLSHGGHLTHGSPVNVSGKWFKVVHYGVERDSQQLDMEAVRQLALKERPQLIICGY
EEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHCCHHHHHHHHHHCCCCEEEECC
SAYPRTIDFAAFRSIADEVGAYLLADMAHIAGLVAAGVHPSPIAHCDVVTTTTHKTLRGP
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCHHHHCCC
RGGLILCRDADFGRKFDKAVFPGSQGGPLEHVIAAKAVALGEALQPEFQVYSCQVVANAQ
CCCEEEEECCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEEECHH
VLAGRIQERGIAVVSGGTDNHLVLLDLRSIGMTGKVADLLVSEVNITANKNTVPFDPESP
HHHHHHHHCCEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHEEEEECCCCCCCCCCCC
FVTSGLRLGTAALTTRGFDDEAFREVADVIADRLLKPQDESIKAQCLERVRQLCGRFPLY
CHHCCCHHCHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHH
RDVLQPALA
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12917642