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The map label for this gene is hemF

Identifier: 33863973

GI number: 33863973

Start: 1805100

End: 1806149

Strand: Reverse

Name: hemF

Synonym: PMT1706

Alternate gene names: 33863973

Gene position: 1806149-1805100 (Counterclockwise)

Preceding gene: 33863984

Following gene: 33863970

Centisome position: 74.92

GC content: 54.0

Gene sequence:

>1050_bases
ATGGGCGCTTCTGAGAATCTTGGCCAAGGTCCTCCTCCTCCTCACTCGCGCAAACGTGCACGTGAATTGGTACTCGGCTT
GCAAGATGAGATCTGTAATGAACTGGAGAGTCTGGATGGTGGCCAATCTTTTAGAACTGACAGTTGGGAGCGGCCTGAAG
GGGGTGGTGGGCGATCCAAGGTGATGCGTGAGGGCCGAGTTTTCGAGCAGGGCGGCGTTAATTTCTCTGAGGTGCACGGC
GAGGAGTTGCCTCCGTCGATTTTGAATCAGCGGCCTGAGGCAAAGGGGCATCCCTGGTTTGCTACCGGCACTTCGATGGT
GCTACACCCGCGCAATCCCTATGTACCTACGATCCACCTGAATTACCGCTATTTCGAGGCGGGGCCGGTTTGGTGGTTTG
GTGGTGGCGCTGACCTCACGCCGTTTTATCCCTACCTGGAAGATGCCCGCCATTTCCATCGCGTTCACAAGCAGGCTTGT
GACACGGTTGGACCTGAGCTCCATAAGGTCTTTAAACCTTGGTGTGACGAATATTTCTATCTGAAGCACCGTGGTGAGAC
ACGTGGAGTGGGTGGAATTTTTTACGACTATCAGGATGGATCTGGAGTGCTTTACAAAGGTCAAAACTCTGAGGGGCCCG
CTGCACAGGTCTCACGGGAGTTAGGGCCTCATCCGAAGAGCTGGGAACAGTTGTTTGAGCTGGCCAAGGCTTGTGGGAAG
GCTTTCTTGCCGGCTTATGTGCCGATTGTGGAGAAACGTCAGGAGCAGGCCTATGGCGATCGAGAACGTCAATTCCAGTT
GTATCGCCGTGGGCGATATGCGGAGTTCAATCTGGTCTGGGATCGGGGCACGATTTTCGGATTGCAAACCAATGGTCGAA
CTGAGTCGATCTTGATGTCTTTGCCACCACTGGCTCGTTGGGAGTATGGATATGTCGCACCAGCTGATTCAAGGGAGGCT
TTGCTCACTGATTTGTTTACTCGACCTCAGAATTGGTTTGAGGATGCGACGTTGGAGGAGCGTTGTCGACCACACCAGGC
GGTGGATTAG

Upstream 100 bases:

>100_bases
CGATATCTGAAAAGACACAAAAACAGAGCTGTCTTGGCACAAAATACAACGCAATTCTATTACTGTTGTCTGCATTGTTC
CTACTTTAATTGGCAAGCTT

Downstream 100 bases:

>100_bases
AAGACGTCTTCCCACGTACTCCCAGCATCAAAGCATCGGTGACTTTTAGGGCGATGGCTTGAATGGTTGCTTCTCTGGTT
TTGGGGTTGCGGAGATTTGC

Product: coproporphyrinogen III oxidase

Products: NA

Alternate protein names: Coprogen oxidase; Coproporphyrinogenase

Number of amino acids: Translated: 349; Mature: 348

Protein sequence:

>349_residues
MGASENLGQGPPPPHSRKRARELVLGLQDEICNELESLDGGQSFRTDSWERPEGGGGRSKVMREGRVFEQGGVNFSEVHG
EELPPSILNQRPEAKGHPWFATGTSMVLHPRNPYVPTIHLNYRYFEAGPVWWFGGGADLTPFYPYLEDARHFHRVHKQAC
DTVGPELHKVFKPWCDEYFYLKHRGETRGVGGIFYDYQDGSGVLYKGQNSEGPAAQVSRELGPHPKSWEQLFELAKACGK
AFLPAYVPIVEKRQEQAYGDRERQFQLYRRGRYAEFNLVWDRGTIFGLQTNGRTESILMSLPPLARWEYGYVAPADSREA
LLTDLFTRPQNWFEDATLEERCRPHQAVD

Sequences:

>Translated_349_residues
MGASENLGQGPPPPHSRKRARELVLGLQDEICNELESLDGGQSFRTDSWERPEGGGGRSKVMREGRVFEQGGVNFSEVHG
EELPPSILNQRPEAKGHPWFATGTSMVLHPRNPYVPTIHLNYRYFEAGPVWWFGGGADLTPFYPYLEDARHFHRVHKQAC
DTVGPELHKVFKPWCDEYFYLKHRGETRGVGGIFYDYQDGSGVLYKGQNSEGPAAQVSRELGPHPKSWEQLFELAKACGK
AFLPAYVPIVEKRQEQAYGDRERQFQLYRRGRYAEFNLVWDRGTIFGLQTNGRTESILMSLPPLARWEYGYVAPADSREA
LLTDLFTRPQNWFEDATLEERCRPHQAVD
>Mature_348_residues
GASENLGQGPPPPHSRKRARELVLGLQDEICNELESLDGGQSFRTDSWERPEGGGGRSKVMREGRVFEQGGVNFSEVHGE
ELPPSILNQRPEAKGHPWFATGTSMVLHPRNPYVPTIHLNYRYFEAGPVWWFGGGADLTPFYPYLEDARHFHRVHKQACD
TVGPELHKVFKPWCDEYFYLKHRGETRGVGGIFYDYQDGSGVLYKGQNSEGPAAQVSRELGPHPKSWEQLFELAKACGKA
FLPAYVPIVEKRQEQAYGDRERQFQLYRRGRYAEFNLVWDRGTIFGLQTNGRTESILMSLPPLARWEYGYVAPADSREAL
LTDLFTRPQNWFEDATLEERCRPHQAVD

Specific function: Key enzyme in heme biosynthesis. Catalyzes the oxidative decarboxylation of propionic acid side chains of rings A and B of coproporphyrinogen III

COG id: COG0408

COG function: function code H; Coproporphyrinogen III oxidase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aerobic coproporphyrinogen-III oxidase family

Homologues:

Organism=Homo sapiens, GI41393599, Length=334, Percent_Identity=43.4131736526946, Blast_Score=280, Evalue=1e-75,
Organism=Escherichia coli, GI1788777, Length=319, Percent_Identity=51.41065830721, Blast_Score=325, Evalue=3e-90,
Organism=Saccharomyces cerevisiae, GI6320249, Length=342, Percent_Identity=43.2748538011696, Blast_Score=261, Evalue=1e-70,
Organism=Drosophila melanogaster, GI17864388, Length=330, Percent_Identity=46.3636363636364, Blast_Score=291, Evalue=3e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM6_PROMM (Q7V568)

Other databases:

- EMBL:   BX548175
- RefSeq:   NP_895533.1
- ProteinModelPortal:   Q7V568
- SMR:   Q7V568
- STRING:   Q7V568
- GeneID:   1728083
- GenomeReviews:   BX548175_GR
- KEGG:   pmt:PMT1706
- NMPDR:   fig|74547.1.peg.1700
- eggNOG:   COG0408
- HOGENOM:   HBG631180
- OMA:   VFKPWCD
- ProtClustDB:   PRK05330
- BioCyc:   PMAR74547:PMT1706-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00333
- InterPro:   IPR001260
- InterPro:   IPR018375
- Gene3D:   G3DSA:3.40.1500.10
- PANTHER:   PTHR10755
- PIRSF:   PIRSF000166
- PRINTS:   PR00073

Pfam domain/function: PF01218 Coprogen_oxidas; SSF102886 Coprogen_oxidas

EC number: =1.3.3.3

Molecular weight: Translated: 39847; Mature: 39716

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS01021 COPROGEN_OXIDASE

Important sites: ACT_SITE 119-119 BINDING 105-105

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGASENLGQGPPPPHSRKRARELVLGLQDEICNELESLDGGQSFRTDSWERPEGGGGRSK
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHH
VMREGRVFEQGGVNFSEVHGEELPPSILNQRPEAKGHPWFATGTSMVLHPRNPYVPTIHL
HHHCCCCHHCCCCCHHHHCCCCCCHHHHCCCCCCCCCCEEECCCEEEEECCCCCCCEEEE
NYRYFEAGPVWWFGGGADLTPFYPYLEDARHFHRVHKQACDTVGPELHKVFKPWCDEYFY
EEEEEECCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHEEE
LKHRGETRGVGGIFYDYQDGSGVLYKGQNSEGPAAQVSRELGPHPKSWEQLFELAKACGK
EEECCCCCCCCEEEEEEECCCEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHH
AFLPAYVPIVEKRQEQAYGDRERQFQLYRRGRYAEFNLVWDRGTIFGLQTNGRTESILMS
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEEEEECCEEEEEECCCCCHHHEEE
LPPLARWEYGYVAPADSREALLTDLFTRPQNWFEDATLEERCRPHQAVD
CCCCCCCCCCEECCCCCHHHHHHHHHHCCHHHHHCCCHHHHCCCCCCCC
>Mature Secondary Structure 
GASENLGQGPPPPHSRKRARELVLGLQDEICNELESLDGGQSFRTDSWERPEGGGGRSK
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHH
VMREGRVFEQGGVNFSEVHGEELPPSILNQRPEAKGHPWFATGTSMVLHPRNPYVPTIHL
HHHCCCCHHCCCCCHHHHCCCCCCHHHHCCCCCCCCCCEEECCCEEEEECCCCCCCEEEE
NYRYFEAGPVWWFGGGADLTPFYPYLEDARHFHRVHKQACDTVGPELHKVFKPWCDEYFY
EEEEEECCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHEEE
LKHRGETRGVGGIFYDYQDGSGVLYKGQNSEGPAAQVSRELGPHPKSWEQLFELAKACGK
EEECCCCCCCCEEEEEEECCCEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHH
AFLPAYVPIVEKRQEQAYGDRERQFQLYRRGRYAEFNLVWDRGTIFGLQTNGRTESILMS
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEEEEECCEEEEEECCCCCHHHEEE
LPPLARWEYGYVAPADSREALLTDLFTRPQNWFEDATLEERCRPHQAVD
CCCCCCCCCCEECCCCCHHHHHHHHHHCCHHHHHCCCHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917642