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The map label for this gene is dnaK [H]

Identifier: 33863711

GI number: 33863711

Start: 1529226

End: 1530851

Strand: Direct

Name: dnaK [H]

Synonym: PMT1444

Alternate gene names: 33863711

Gene position: 1529226-1530851 (Clockwise)

Preceding gene: 33863706

Following gene: 33863713

Centisome position: 63.43

GC content: 59.1

Gene sequence:

>1626_bases
ATGGCCATGGACATGCCCCCAGAGGGCGAAGAAAAGCTTCAGGTTTTCCAACAAGTTGGCACCCTTGCCATTGACCTTGG
GAACACCACCACGGTTGTGGCCTTCCAGGCTGAACGTGCTGCCAGCCCTCAACTCCTCGATCTCCCACCAATCAGCCGTC
GCCCAGGAGAAGTCCCAAGTTTGATCTGGGCCGGCCAAGGCAATGACCCCAACCCTCTAGTGGGGCAGCAAGTGGACGAA
GCAGGCCTGGTGGGGCAGGGTCACACAAGCTTGAGCAGAGACTTCAAGCGCTGGATCGGTGCACCTCATCCAAGCAAAAG
CGATGGCTCTTGCCTCTCGCCAGAACAGGCCGGAGAAATCTTGCTGCAGCAGATCTGGCGCCGACTCCCTCCCCATATCG
AGGTGCGACGTCTTGTGCTCACTGCGCCAGTGGAGAGCTATCGCGCCTATAGAACCTGGCTGAATCAGGTCTGCACAACA
CTGCCGGTTGAAGAGATCGCCCTGGTTGATGAACCCACCGCTGCTGCCATGGGTGCCGGCCTACCCCCAGGCTCCAAGCT
GTTGGTGATGGACATCGGTGGCAGCACCATTGATCTATCCCTCGTGGCCCTCGAAGGTGGTGAAGGACGGGCGGCTCCAA
TCGCCCAATTACTTCGATTTGCTGGCCAAGATCTTGAGGACAGCAGCAAACAAGCCCTGCGCTGCGCACGCGTGCTTGGC
AAGGCTGGACTGAAGTTGGGTGGACGGGACCTCGATCATTGGATTGCCAATCATCTTTATCCCGACGTTCCGCTCTCAGA
AACACTCCTCAATGCTGCAGAGAGGCTCAAATGCCGCCTTAGCCAGGTGGATCTTCGCGCTGAGGCCACCCAGCTGGAGA
TTGCCGCCGATTCAGAAGGCAATGAAGTCCTGCCTCTACGTCTCTGCCGCAGGGAGCTGGAAGAGCTGCTCATCGCCAGA
GGCTTGCTTAACAGCCTGGCCAGCCTGCTCAATGAAACCCTGGCAAGGGGGCGTGGCAATGGTTGCGAACTCAAAGACCT
ACAGGGGGTCGTAGCCGTCGGCGGCGGCGCACAAATCCCCCTCGTTCGCAGTTGGTTGCAGCAGCAAACACAACCAGCCC
CACTCCTCACGCCTCCCCCCATAGAGGCCGTTGCTGTTGGAGCCCTGCAACTCACACCGGGGGTCAAAGTTCAGGATGTA
CTGCACCGCGGAGTCTCTCTGCGCTGCTGGGATCAGCGAGGTGGCCAACATCATTGGCATCCGCTGTTCCTCGCTGGTCA
GCCCTGGCCAACAACCGCCCCCCTTGAGCTTGTGCTGGCCGCCAGCCGGATCGATCAACTGGAGCTAGAGGTGGTGCTTG
CTGAGCCAGACATCAACGAGAGCCATGAGGTCATCTACATCGATGGAATGCCAACCCTGCAATCAAAGCCAGCAGAGCGC
AAACTACATCCCTGGCCAGGAACCTGCCCTTCAGCTTCGCTTGCACTACAACCACCCGGTCAGCCGGGGCAAGACTGCCT
GAGGTTGCAATTCTCCATCGATGACGATGCCCAGCTGCAGATGGAGGGGCTAGATCTACGCAGTGGTGAGCGGCTAGGCA
AGCAAAAGCTTGGCACCGTGCAGTAA

Upstream 100 bases:

>100_bases
TACCTCAATGCAAGGGTCCTCTCGATAAAAAGAGAGCACCAAGCGCTGCAACCGATCCATCGTCATTGTCTCTTAGCCTA
GTAATGACTACAACCTGGCC

Downstream 100 bases:

>100_bases
CTGATTCATCCACGCCAACCTCAACGCAATCCAAAGGCAAGACGACCATGGGCTGAACTGGCCTCTGTCATCAAGACGAA
CAACGCAGATGCATGAACAC

Product: HSP70 family molecular chaperone

Products: NA

Alternate protein names: HSP70; Heat shock 70 kDa protein; Heat shock protein 70 [H]

Number of amino acids: Translated: 541; Mature: 540

Protein sequence:

>541_residues
MAMDMPPEGEEKLQVFQQVGTLAIDLGNTTTVVAFQAERAASPQLLDLPPISRRPGEVPSLIWAGQGNDPNPLVGQQVDE
AGLVGQGHTSLSRDFKRWIGAPHPSKSDGSCLSPEQAGEILLQQIWRRLPPHIEVRRLVLTAPVESYRAYRTWLNQVCTT
LPVEEIALVDEPTAAAMGAGLPPGSKLLVMDIGGSTIDLSLVALEGGEGRAAPIAQLLRFAGQDLEDSSKQALRCARVLG
KAGLKLGGRDLDHWIANHLYPDVPLSETLLNAAERLKCRLSQVDLRAEATQLEIAADSEGNEVLPLRLCRRELEELLIAR
GLLNSLASLLNETLARGRGNGCELKDLQGVVAVGGGAQIPLVRSWLQQQTQPAPLLTPPPIEAVAVGALQLTPGVKVQDV
LHRGVSLRCWDQRGGQHHWHPLFLAGQPWPTTAPLELVLAASRIDQLELEVVLAEPDINESHEVIYIDGMPTLQSKPAER
KLHPWPGTCPSASLALQPPGQPGQDCLRLQFSIDDDAQLQMEGLDLRSGERLGKQKLGTVQ

Sequences:

>Translated_541_residues
MAMDMPPEGEEKLQVFQQVGTLAIDLGNTTTVVAFQAERAASPQLLDLPPISRRPGEVPSLIWAGQGNDPNPLVGQQVDE
AGLVGQGHTSLSRDFKRWIGAPHPSKSDGSCLSPEQAGEILLQQIWRRLPPHIEVRRLVLTAPVESYRAYRTWLNQVCTT
LPVEEIALVDEPTAAAMGAGLPPGSKLLVMDIGGSTIDLSLVALEGGEGRAAPIAQLLRFAGQDLEDSSKQALRCARVLG
KAGLKLGGRDLDHWIANHLYPDVPLSETLLNAAERLKCRLSQVDLRAEATQLEIAADSEGNEVLPLRLCRRELEELLIAR
GLLNSLASLLNETLARGRGNGCELKDLQGVVAVGGGAQIPLVRSWLQQQTQPAPLLTPPPIEAVAVGALQLTPGVKVQDV
LHRGVSLRCWDQRGGQHHWHPLFLAGQPWPTTAPLELVLAASRIDQLELEVVLAEPDINESHEVIYIDGMPTLQSKPAER
KLHPWPGTCPSASLALQPPGQPGQDCLRLQFSIDDDAQLQMEGLDLRSGERLGKQKLGTVQ
>Mature_540_residues
AMDMPPEGEEKLQVFQQVGTLAIDLGNTTTVVAFQAERAASPQLLDLPPISRRPGEVPSLIWAGQGNDPNPLVGQQVDEA
GLVGQGHTSLSRDFKRWIGAPHPSKSDGSCLSPEQAGEILLQQIWRRLPPHIEVRRLVLTAPVESYRAYRTWLNQVCTTL
PVEEIALVDEPTAAAMGAGLPPGSKLLVMDIGGSTIDLSLVALEGGEGRAAPIAQLLRFAGQDLEDSSKQALRCARVLGK
AGLKLGGRDLDHWIANHLYPDVPLSETLLNAAERLKCRLSQVDLRAEATQLEIAADSEGNEVLPLRLCRRELEELLIARG
LLNSLASLLNETLARGRGNGCELKDLQGVVAVGGGAQIPLVRSWLQQQTQPAPLLTPPPIEAVAVGALQLTPGVKVQDVL
HRGVSLRCWDQRGGQHHWHPLFLAGQPWPTTAPLELVLAASRIDQLELEVVLAEPDINESHEVIYIDGMPTLQSKPAERK
LHPWPGTCPSASLALQPPGQPGQDCLRLQFSIDDDAQLQMEGLDLRSGERLGKQKLGTVQ

Specific function: Acts as a chaperone [H]

COG id: COG0443

COG function: function code O; Molecular chaperone

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the heat shock protein 70 family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6319972, Length=413, Percent_Identity=25.6658595641646, Blast_Score=68, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6322426, Length=294, Percent_Identity=26.530612244898, Blast_Score=68, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6324120, Length=413, Percent_Identity=25.6658595641646, Blast_Score=68, Evalue=3e-12,

Paralogues:

None

Copy number: 1300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 320 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 500 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stati

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012725
- InterPro:   IPR018181
- InterPro:   IPR001023
- InterPro:   IPR013126 [H]

Pfam domain/function: PF00012 HSP70 [H]

EC number: NA

Molecular weight: Translated: 58529; Mature: 58398

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00329 HSP70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAMDMPPEGEEKLQVFQQVGTLAIDLGNTTTVVAFQAERAASPQLLDLPPISRRPGEVPS
CCCCCCCCCHHHHHHHHHHCCEEEECCCCEEEEEEECCCCCCCCEECCCCCCCCCCCCCC
LIWAGQGNDPNPLVGQQVDEAGLVGQGHTSLSRDFKRWIGAPHPSKSDGSCLSPEQAGEI
EEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHH
LLQQIWRRLPPHIEVRRLVLTAPVESYRAYRTWLNQVCTTLPVEEIALVDEPTAAAMGAG
HHHHHHHHCCCCCEEEHEEEECCHHHHHHHHHHHHHHHHHCCHHHHEECCCCCHHHHCCC
LPPGSKLLVMDIGGSTIDLSLVALEGGEGRAAPIAQLLRFAGQDLEDSSKQALRCARVLG
CCCCCEEEEEECCCCEEEEEEEEEECCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
KAGLKLGGRDLDHWIANHLYPDVPLSETLLNAAERLKCRLSQVDLRAEATQLEIAADSEG
HHCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCC
NEVLPLRLCRRELEELLIARGLLNSLASLLNETLARGRGNGCELKDLQGVVAVGGGAQIP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCEEEECCCCCCH
LVRSWLQQQTQPAPLLTPPPIEAVAVGALQLTPGVKVQDVLHRGVSLRCWDQRGGQHHWH
HHHHHHHHCCCCCCCCCCCCHHHHEECEEEECCCCHHHHHHHCCCEEEECCCCCCCCCCC
PLFLAGQPWPTTAPLELVLAASRIDQLELEVVLAEPDINESHEVIYIDGMPTLQSKPAER
EEEEECCCCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCCEEEECCCCCCCCCCCCC
KLHPWPGTCPSASLALQPPGQPGQDCLRLQFSIDDDAQLQMEGLDLRSGERLGKQKLGTV
CCCCCCCCCCCCCEEECCCCCCCCCCEEEEEECCCCCEEEEECCCCCCCHHHHHHHCCCC
Q
C
>Mature Secondary Structure 
AMDMPPEGEEKLQVFQQVGTLAIDLGNTTTVVAFQAERAASPQLLDLPPISRRPGEVPS
CCCCCCCCHHHHHHHHHHCCEEEECCCCEEEEEEECCCCCCCCEECCCCCCCCCCCCCC
LIWAGQGNDPNPLVGQQVDEAGLVGQGHTSLSRDFKRWIGAPHPSKSDGSCLSPEQAGEI
EEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHH
LLQQIWRRLPPHIEVRRLVLTAPVESYRAYRTWLNQVCTTLPVEEIALVDEPTAAAMGAG
HHHHHHHHCCCCCEEEHEEEECCHHHHHHHHHHHHHHHHHCCHHHHEECCCCCHHHHCCC
LPPGSKLLVMDIGGSTIDLSLVALEGGEGRAAPIAQLLRFAGQDLEDSSKQALRCARVLG
CCCCCEEEEEECCCCEEEEEEEEEECCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
KAGLKLGGRDLDHWIANHLYPDVPLSETLLNAAERLKCRLSQVDLRAEATQLEIAADSEG
HHCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCC
NEVLPLRLCRRELEELLIARGLLNSLASLLNETLARGRGNGCELKDLQGVVAVGGGAQIP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCEEEECCCCCCH
LVRSWLQQQTQPAPLLTPPPIEAVAVGALQLTPGVKVQDVLHRGVSLRCWDQRGGQHHWH
HHHHHHHHCCCCCCCCCCCCHHHHEECEEEECCCCHHHHHHHCCCEEEECCCCCCCCCCC
PLFLAGQPWPTTAPLELVLAASRIDQLELEVVLAEPDINESHEVIYIDGMPTLQSKPAER
EEEEECCCCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCCEEEECCCCCCCCCCCCC
KLHPWPGTCPSASLALQPPGQPGQDCLRLQFSIDDDAQLQMEGLDLRSGERLGKQKLGTV
CCCCCCCCCCCCCEEECCCCCCCCCCEEEEEECCCCCEEEEECCCCCCCHHHHHHHCCCC
Q
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA