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The map label for this gene is gloB [H]

Identifier: 33863464

GI number: 33863464

Start: 1284495

End: 1285277

Strand: Direct

Name: gloB [H]

Synonym: PMT1194

Alternate gene names: 33863464

Gene position: 1284495-1285277 (Clockwise)

Preceding gene: 33863460

Following gene: 33863465

Centisome position: 53.28

GC content: 52.62

Gene sequence:

>783_bases
ATGGAGGCAACCTCAACAGCTGCGACTAGCGATAAACAACGTTCATCGATCCATGCCCTTCCTGTTCTTCAGGACAACAT
CATCTGGATCTGGATTAAAGGCAACCAGGCTGTCGTTGTGGATCCAGCAATCGCAGAACCAGTCAAGACATGGCTGCGAA
CTCGAAAGCTCTCACTCGCAGCAGTACTTCAAACCCATCACCATGCTGATCACATTGGTGGCACCCTTGAGCTGCTTAGG
GACTGGCCTAACGCAGCCGTGGTAGCAGCCGCTGAAGACCGTGATCGCATTCCCTTCCAGACGATCTCCGTTCGTGATAG
AGACAAGGTTTCTCTTCTCAACAGCAGTGTTGAAGTGCTCGCTGTTGCGGGTCATACCAGAGCACACATTGCTTATTACC
TGCCCACAAACAAGGAAGATCTTGAAGATCCAGCAGTGTTCTGTGGTGACACCCTTTTTGGAGCTGGATGTGGCCGACTG
TTTGAAGGAACTGCCGAAGACATGTTCAAGGCTCTTCAACGTCTCAGTTGCCTGCCAGCGAAGACCCGTGTGTATTGCGC
TCACGAATACACCGAAGCAAATCTGCGCTGGGCCTCCGCCCTGCATCCAGAAGATGTTGCTATCAGCGAACGACTGGCAG
ACGTTTCAAGTCGCCGCCAGAGGGGTGCCCTGAGCCTGCCTAGCAGCATTTCAGAAGAACAACGTACCAACCTCTTCGTG
CGTGCTCAGAGCAGCAAAGAGCTAGCAGAACTTCGCCACCACAAGGATCAATGGCGAAACTAA

Upstream 100 bases:

>100_bases
AACCCAGCAGATTGAAAACGAGCAACCGAATCATTCAGCAAAGCTCCTTTTGCAAGCGCGACAGTGATCATGGAGCGATG
CGTCGACCTGGACTTTAACG

Downstream 100 bases:

>100_bases
GCACAAATATCTGACTCAGCAACAAGGCAGTCCTGTTGCAATGGAACAGGACCAACAGCTAGTGAGATTGCAATCCATTG
AGATCAAGGCCGCTGGAACA

Product: hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II [H]

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MEATSTAATSDKQRSSIHALPVLQDNIIWIWIKGNQAVVVDPAIAEPVKTWLRTRKLSLAAVLQTHHHADHIGGTLELLR
DWPNAAVVAAAEDRDRIPFQTISVRDRDKVSLLNSSVEVLAVAGHTRAHIAYYLPTNKEDLEDPAVFCGDTLFGAGCGRL
FEGTAEDMFKALQRLSCLPAKTRVYCAHEYTEANLRWASALHPEDVAISERLADVSSRRQRGALSLPSSISEEQRTNLFV
RAQSSKELAELRHHKDQWRN

Sequences:

>Translated_260_residues
MEATSTAATSDKQRSSIHALPVLQDNIIWIWIKGNQAVVVDPAIAEPVKTWLRTRKLSLAAVLQTHHHADHIGGTLELLR
DWPNAAVVAAAEDRDRIPFQTISVRDRDKVSLLNSSVEVLAVAGHTRAHIAYYLPTNKEDLEDPAVFCGDTLFGAGCGRL
FEGTAEDMFKALQRLSCLPAKTRVYCAHEYTEANLRWASALHPEDVAISERLADVSSRRQRGALSLPSSISEEQRTNLFV
RAQSSKELAELRHHKDQWRN
>Mature_260_residues
MEATSTAATSDKQRSSIHALPVLQDNIIWIWIKGNQAVVVDPAIAEPVKTWLRTRKLSLAAVLQTHHHADHIGGTLELLR
DWPNAAVVAAAEDRDRIPFQTISVRDRDKVSLLNSSVEVLAVAGHTRAHIAYYLPTNKEDLEDPAVFCGDTLFGAGCGRL
FEGTAEDMFKALQRLSCLPAKTRVYCAHEYTEANLRWASALHPEDVAISERLADVSSRRQRGALSLPSSISEEQRTNLFV
RAQSSKELAELRHHKDQWRN

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid [H]

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family [H]

Homologues:

Organism=Homo sapiens, GI94538322, Length=266, Percent_Identity=36.0902255639098, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI94538320, Length=230, Percent_Identity=38.2608695652174, Blast_Score=150, Evalue=8e-37,
Organism=Homo sapiens, GI14150041, Length=228, Percent_Identity=35.5263157894737, Blast_Score=132, Evalue=2e-31,
Organism=Homo sapiens, GI116642887, Length=225, Percent_Identity=35.1111111111111, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI21703352, Length=225, Percent_Identity=35.1111111111111, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI46361987, Length=205, Percent_Identity=36.0975609756098, Blast_Score=119, Evalue=3e-27,
Organism=Escherichia coli, GI1786406, Length=229, Percent_Identity=36.6812227074236, Blast_Score=160, Evalue=9e-41,
Organism=Caenorhabditis elegans, GI17536925, Length=229, Percent_Identity=35.3711790393013, Blast_Score=146, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6320478, Length=231, Percent_Identity=32.9004329004329, Blast_Score=101, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6324614, Length=186, Percent_Identity=31.7204301075269, Blast_Score=84, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24667711, Length=232, Percent_Identity=38.7931034482759, Blast_Score=162, Evalue=3e-40,
Organism=Drosophila melanogaster, GI21356335, Length=232, Percent_Identity=38.7931034482759, Blast_Score=162, Evalue=3e-40,
Organism=Drosophila melanogaster, GI24667703, Length=259, Percent_Identity=36.2934362934363, Blast_Score=160, Evalue=5e-40,
Organism=Drosophila melanogaster, GI221330176, Length=166, Percent_Identity=28.9156626506024, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR017782 [H]

Pfam domain/function: PF00753 Lactamase_B [H]

EC number: =3.1.2.6 [H]

Molecular weight: Translated: 28990; Mature: 28990

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEATSTAATSDKQRSSIHALPVLQDNIIWIWIKGNQAVVVDPAIAEPVKTWLRTRKLSLA
CCCCCCCCCCCHHHCCEEECEEEECCEEEEEEECCEEEEECCHHHHHHHHHHHHHHHHHH
AVLQTHHHADHIGGTLELLRDWPNAAVVAAAEDRDRIPFQTISVRDRDKVSLLNSSVEVL
HHHHHHHHHHHCCHHHHHHHCCCCCEEEEECCCCCCCCEEEECCCCCHHHHHHCCCEEEE
AVAGHTRAHIAYYLPTNKEDLEDPAVFCGDTLFGAGCGRLFEGTAEDMFKALQRLSCLPA
EEECCCEEEEEEEECCCCCCCCCCHHHHCCCHHHCCCHHHHCCCHHHHHHHHHHHHCCCC
KTRVYCAHEYTEANLRWASALHPEDVAISERLADVSSRRQRGALSLPSSISEEQRTNLFV
CCEEEEEECCCCCCEEEHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCEEEE
RAQSSKELAELRHHKDQWRN
EECCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEATSTAATSDKQRSSIHALPVLQDNIIWIWIKGNQAVVVDPAIAEPVKTWLRTRKLSLA
CCCCCCCCCCCHHHCCEEECEEEECCEEEEEEECCEEEEECCHHHHHHHHHHHHHHHHHH
AVLQTHHHADHIGGTLELLRDWPNAAVVAAAEDRDRIPFQTISVRDRDKVSLLNSSVEVL
HHHHHHHHHHHCCHHHHHHHCCCCCEEEEECCCCCCCCEEEECCCCCHHHHHHCCCEEEE
AVAGHTRAHIAYYLPTNKEDLEDPAVFCGDTLFGAGCGRLFEGTAEDMFKALQRLSCLPA
EEECCCEEEEEEEECCCCCCCCCCHHHHCCCHHHCCCHHHHCCCHHHHHHHHHHHHCCCC
KTRVYCAHEYTEANLRWASALHPEDVAISERLADVSSRRQRGALSLPSSISEEQRTNLFV
CCEEEEEECCCCCCEEEHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCEEEE
RAQSSKELAELRHHKDQWRN
EECCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA