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The map label for this gene is gsiB [H]

Identifier: 33863416

GI number: 33863416

Start: 1233653

End: 1235242

Strand: Direct

Name: gsiB [H]

Synonym: PMT1145

Alternate gene names: 33863416

Gene position: 1233653-1235242 (Clockwise)

Preceding gene: 33863415

Following gene: 33863417

Centisome position: 51.17

GC content: 56.92

Gene sequence:

>1590_bases
GTGTCAAGCTCTGGCCGTCGTGATTCTCTGCTCTGGGCCGCTGCTCTATTTGCTTGCTCCCTAGCGCTGAGCCAGGTTGC
CTGTCAGCCGCCTCGCCGTAGTGAGCGACTTGTCGTGGCCAGTGCCGGCAAGATCACCTCTCTCGATCCTGCCCAGGCCA
GTACTTTTGATGCTCTGCAACTGTTGAGCGCCCTCGGAGAGCCCCTCTACCAACTTGATCACAAAGGGGAGCTAGAGCCA
CGACTGGCATCCGCCCCACCTCAGATAAGTGATGGCGGCTTCACCATCTCGATCCCTCTGCGCAGGGATGTGCTGTTTCA
CGACGGCACCCAATTCAACGCCGCCGCGATGGCCTTCAGCCTGCGGCGATTTCTGAGTATCGGCACCCTCAACTACGTGG
TAGGAGGACGGATTGCCGCCGTGGAGACAGCCGGTCCCTACCTGCTACGCCTTCGACTAACACGACCCTCAACTTCTCTA
GAGGGACTACTCAGCTCAATCAATCTGACGCCGGTATCACCAACGGCCTACGCCAAGCACAGAGATCAGTTCCTCAATAA
GCAATTCATTGGAACCGGCCCTTACCGACTCACTAGCTTCCAGACCCAGCAACAACGCCTTGAGCCTTTTCAGCAGTACT
GGAGTACCCAGGCCAGTAATGCTGGAATCGATTTCATCAATCTGAGCAACTCCACGGCCCTATTCGGTGCCCTACGAAGC
GGTGAAGTGGATGTACTCCTTTCCAATTCTCTGGATGAAGACCAACGCCTTGCCCTCCATCGCCTTGCCAAGCAAGGGAA
GCTCCGCGAAGGAGAGGGACCAGCACTTGAGATCGGCTACATCACTCTCCTGAGTAATGCCCCTCCACTCAATCAACCCC
TCTTACGTCAGGCCCTCGCCTACAGCCTCGACCGTCAGCTGATGGTGAAGCGCGTGAGCTATGGACTAAGACGCCCGCTG
CGGTCTTTAGTGCCGCCAAACTTGCAGGCTGAACCACTCACACCTTGGCCCAGCTACAACCCTCAACGAGCCAAACAGCT
GCTGAAAAAGGCAGGCTATTGCGGAACTCAAACGCTGACACTACCTTTTACTTTCCGTTCCAACGTGCCAGCAGACAAGT
TATTGGCGCTTACATGGCAGGCACAGGTGGAACGTGATCTCTCGGATTGCCTGACACTGAAGCTCAATAGCGTCGAATCG
ACCACCGTTTATCGCCAACTGGGGGAAGGCGCATTCCAGGCCGTCATCCTTGAGTGGCGAGGCGCCTATCCCGACCCGGA
AGCCTATTTAGCTCCATTATTAAGCTGCAGCAAAGCCAATGGATCTGTATGCGAAGAAGGGGAAGCGGCAATTAGCGGCA
GCTTCTGGACCGCAGATGGGCTGGAAACGAGCCTTCGCTACAGCGATGAACTTCGCGGACCCGATCGTCTCCACCAGCTC
AGAGAGATCGAACGCTCCGCCGCCGGCGGAGCGGCCTATCTACCAATCTGGCTGGTAGCTCCACGAGCTTGGGCCCAGCG
GCGGTTATCCAAACCAGAGTTTGACGGGAGCGGCCATTTAATGCTCCAGCATCTACGGGAGCTGCACTGA

Upstream 100 bases:

>100_bases
GTTTGAAGTGTCGATACAACAAATCTCCTGCGCCTCCAAGTAAACAGAACGACACTCACTAGCAAGTCTTTAAATCAAGG
CCATCCGATCCGCCCGCGTT

Downstream 100 bases:

>100_bases
TGGGCCGCGGGAGAGAACTACTGCGCTACAGCTCGACCCGCTTGGCCCTGGCACCGGTGATGCTCTGGCTCATTGCCAGC
ATGGTGTTTCTCCTCCTACG

Product: ABC transporter substrate-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 529; Mature: 528

Protein sequence:

>529_residues
MSSSGRRDSLLWAAALFACSLALSQVACQPPRRSERLVVASAGKITSLDPAQASTFDALQLLSALGEPLYQLDHKGELEP
RLASAPPQISDGGFTISIPLRRDVLFHDGTQFNAAAMAFSLRRFLSIGTLNYVVGGRIAAVETAGPYLLRLRLTRPSTSL
EGLLSSINLTPVSPTAYAKHRDQFLNKQFIGTGPYRLTSFQTQQQRLEPFQQYWSTQASNAGIDFINLSNSTALFGALRS
GEVDVLLSNSLDEDQRLALHRLAKQGKLREGEGPALEIGYITLLSNAPPLNQPLLRQALAYSLDRQLMVKRVSYGLRRPL
RSLVPPNLQAEPLTPWPSYNPQRAKQLLKKAGYCGTQTLTLPFTFRSNVPADKLLALTWQAQVERDLSDCLTLKLNSVES
TTVYRQLGEGAFQAVILEWRGAYPDPEAYLAPLLSCSKANGSVCEEGEAAISGSFWTADGLETSLRYSDELRGPDRLHQL
REIERSAAGGAAYLPIWLVAPRAWAQRRLSKPEFDGSGHLMLQHLRELH

Sequences:

>Translated_529_residues
MSSSGRRDSLLWAAALFACSLALSQVACQPPRRSERLVVASAGKITSLDPAQASTFDALQLLSALGEPLYQLDHKGELEP
RLASAPPQISDGGFTISIPLRRDVLFHDGTQFNAAAMAFSLRRFLSIGTLNYVVGGRIAAVETAGPYLLRLRLTRPSTSL
EGLLSSINLTPVSPTAYAKHRDQFLNKQFIGTGPYRLTSFQTQQQRLEPFQQYWSTQASNAGIDFINLSNSTALFGALRS
GEVDVLLSNSLDEDQRLALHRLAKQGKLREGEGPALEIGYITLLSNAPPLNQPLLRQALAYSLDRQLMVKRVSYGLRRPL
RSLVPPNLQAEPLTPWPSYNPQRAKQLLKKAGYCGTQTLTLPFTFRSNVPADKLLALTWQAQVERDLSDCLTLKLNSVES
TTVYRQLGEGAFQAVILEWRGAYPDPEAYLAPLLSCSKANGSVCEEGEAAISGSFWTADGLETSLRYSDELRGPDRLHQL
REIERSAAGGAAYLPIWLVAPRAWAQRRLSKPEFDGSGHLMLQHLRELH
>Mature_528_residues
SSSGRRDSLLWAAALFACSLALSQVACQPPRRSERLVVASAGKITSLDPAQASTFDALQLLSALGEPLYQLDHKGELEPR
LASAPPQISDGGFTISIPLRRDVLFHDGTQFNAAAMAFSLRRFLSIGTLNYVVGGRIAAVETAGPYLLRLRLTRPSTSLE
GLLSSINLTPVSPTAYAKHRDQFLNKQFIGTGPYRLTSFQTQQQRLEPFQQYWSTQASNAGIDFINLSNSTALFGALRSG
EVDVLLSNSLDEDQRLALHRLAKQGKLREGEGPALEIGYITLLSNAPPLNQPLLRQALAYSLDRQLMVKRVSYGLRRPLR
SLVPPNLQAEPLTPWPSYNPQRAKQLLKKAGYCGTQTLTLPFTFRSNVPADKLLALTWQAQVERDLSDCLTLKLNSVEST
TVYRQLGEGAFQAVILEWRGAYPDPEAYLAPLLSCSKANGSVCEEGEAAISGSFWTADGLETSLRYSDELRGPDRLHQLR
EIERSAAGGAAYLPIWLVAPRAWAQRRLSKPEFDGSGHLMLQHLRELH

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=359, Percent_Identity=28.4122562674095, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1789966, Length=467, Percent_Identity=23.7687366167024, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1787762, Length=455, Percent_Identity=23.2967032967033, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1787551, Length=434, Percent_Identity=23.7327188940092, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1789887, Length=543, Percent_Identity=23.9410681399632, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1787495, Length=457, Percent_Identity=23.1947483588621, Blast_Score=86, Evalue=8e-18,
Organism=Escherichia coli, GI1789397, Length=464, Percent_Identity=24.3534482758621, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87081878, Length=469, Percent_Identity=22.8144989339019, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 58282; Mature: 58151

Theoretical pI: Translated: 9.06; Mature: 9.06

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSSGRRDSLLWAAALFACSLALSQVACQPPRRSERLVVASAGKITSLDPAQASTFDALQ
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHH
LLSALGEPLYQLDHKGELEPRLASAPPQISDGGFTISIPLRRDVLFHDGTQFNAAAMAFS
HHHHHCCHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEECEECEEEECCCCCHHHHHHHH
LRRFLSIGTLNYVVGGRIAAVETAGPYLLRLRLTRPSTSLEGLLSSINLTPVSPTAYAKH
HHHHHHHCHHHHHHCCEEEEEECCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCHHHHHH
RDQFLNKQFIGTGPYRLTSFQTQQQRLEPFQQYWSTQASNAGIDFINLSNSTALFGALRS
HHHHHCCCCCCCCCEEECCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEEHEECCC
GEVDVLLSNSLDEDQRLALHRLAKQGKLREGEGPALEIGYITLLSNAPPLNQPLLRQALA
CCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCCCCHHHHHHHHH
YSLDRQLMVKRVSYGLRRPLRSLVPPNLQAEPLTPWPSYNPQRAKQLLKKAGYCGTQTLT
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEE
LPFTFRSNVPADKLLALTWQAQVERDLSDCLTLKLNSVESTTVYRQLGEGAFQAVILEWR
EEEEECCCCCHHHHEEEHHHHHHHHHHHHHHEEEECCCHHHHHHHHHCCHHHEEEEEEEC
GAYPDPEAYLAPLLSCSKANGSVCEEGEAAISGSFWTADGLETSLRYSDELRGPDRLHQL
CCCCCHHHHHHHHHHHCCCCCCCCCCCCCEECCCEEECCCCHHHHCCCHHCCCHHHHHHH
REIERSAAGGAAYLPIWLVAPRAWAQRRLSKPEFDGSGHLMLQHLRELH
HHHHHHCCCCCEEEEEEEECCHHHHHHHCCCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
SSSGRRDSLLWAAALFACSLALSQVACQPPRRSERLVVASAGKITSLDPAQASTFDALQ
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHH
LLSALGEPLYQLDHKGELEPRLASAPPQISDGGFTISIPLRRDVLFHDGTQFNAAAMAFS
HHHHHCCHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEECEECEEEECCCCCHHHHHHHH
LRRFLSIGTLNYVVGGRIAAVETAGPYLLRLRLTRPSTSLEGLLSSINLTPVSPTAYAKH
HHHHHHHCHHHHHHCCEEEEEECCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCHHHHHH
RDQFLNKQFIGTGPYRLTSFQTQQQRLEPFQQYWSTQASNAGIDFINLSNSTALFGALRS
HHHHHCCCCCCCCCEEECCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEEHEECCC
GEVDVLLSNSLDEDQRLALHRLAKQGKLREGEGPALEIGYITLLSNAPPLNQPLLRQALA
CCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCCCCHHHHHHHHH
YSLDRQLMVKRVSYGLRRPLRSLVPPNLQAEPLTPWPSYNPQRAKQLLKKAGYCGTQTLT
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEE
LPFTFRSNVPADKLLALTWQAQVERDLSDCLTLKLNSVESTTVYRQLGEGAFQAVILEWR
EEEEECCCCCHHHHEEEHHHHHHHHHHHHHHEEEECCCHHHHHHHHHCCHHHEEEEEEEC
GAYPDPEAYLAPLLSCSKANGSVCEEGEAAISGSFWTADGLETSLRYSDELRGPDRLHQL
CCCCCHHHHHHHHHHHCCCCCCCCCCCCCEECCCEEECCCCHHHHCCCHHCCCHHHHHHH
REIERSAAGGAAYLPIWLVAPRAWAQRRLSKPEFDGSGHLMLQHLRELH
HHHHHHCCCCCEEEEEEEECCHHHHHHHCCCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA