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The map label for this gene is sphR [H]

Identifier: 33863368

GI number: 33863368

Start: 1187783

End: 1188556

Strand: Direct

Name: sphR [H]

Synonym: PMT1097

Alternate gene names: 33863368

Gene position: 1187783-1188556 (Clockwise)

Preceding gene: 33863366

Following gene: 33863370

Centisome position: 49.27

GC content: 54.78

Gene sequence:

>774_bases
GTGACCTCCATTCCCCGCAATCTTCATTCCAATGAGGACACCCAAGTGTCTTCATCGGGGTCTTTGCAGATGCAGACCAC
GCCAAAGCAGCCCTCAAGGGTCCTTGTGGTGGAACCTCACCAAACTCTTCGCACGGTGCTGGTCCATCGCCTCCGTCAGG
ACGGCCATCTCACCGCTGCAGTGGCATCAGCCGTGGAAGCCCTTGACGTCTGCCGAGATCAATCACCGGATCTTCTAGTT
AGTGCTGAGTTACTTGAGAGGAGTTCTGCAATGCGACTCTCTCATCAGCTCCGTTGCCCTGTGATCGTGTTAACAGCTCG
AACAGGCACAGAGCCCATGGTGGGCCTCCTGAATGAAGGCGCCGACGATGTGTTGCGCAAACCATTTGGACTTGAAGAAC
TCGCCGCACGCTGTCGCACCTTGCTGAAACGGGGACATAGCGGTTTACAGGAGCGCGTCACAGTGGGCCCCCTTGAAGTC
CACTTGTTGCTCAGACAAGTGACTCTGCGCGATAAACCTGTGGAATTAAGCCCCAGAGAATTTGCACTTCTCTGTGCACT
TTTAATGCCTCCAGGTATCGTACGAAGCCGCCAAGAATTATTGCGAATGGCTTGGCCTCCCTTCAGCGGCGGGCCTCGTT
CTGTAGACACCCAGATCCTTACCTTGCGACGCAAACTTGAGCAAGCTGGGCTTGGCGAAGGAGGTGGCATCACCACCATG
CGCCAGCAGGGCTATAGATTCAGCCTGGATACTCTTCCTGATTCTGAATCATAA

Upstream 100 bases:

>100_bases
TCAGGGTGATCAAGAGATGTCATGGTCGGAGATCACGGCAGCCTCTTTTGGCAGCTTGCCATTGGATTGGGTTTAGCGTA
AGCGCGATCTGTGATCACAT

Downstream 100 bases:

>100_bases
ATATTGAACCTCACAACTATCGATTAGCCAATGATTAAAGGCCCTGGCTGAAGTGGCCGAAACAGATTCTAGGCATTACT
AGAGGTTGATGATTTATAAT

Product: two-component response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 257; Mature: 256

Protein sequence:

>257_residues
MTSIPRNLHSNEDTQVSSSGSLQMQTTPKQPSRVLVVEPHQTLRTVLVHRLRQDGHLTAAVASAVEALDVCRDQSPDLLV
SAELLERSSAMRLSHQLRCPVIVLTARTGTEPMVGLLNEGADDVLRKPFGLEELAARCRTLLKRGHSGLQERVTVGPLEV
HLLLRQVTLRDKPVELSPREFALLCALLMPPGIVRSRQELLRMAWPPFSGGPRSVDTQILTLRRKLEQAGLGEGGGITTM
RQQGYRFSLDTLPDSES

Sequences:

>Translated_257_residues
MTSIPRNLHSNEDTQVSSSGSLQMQTTPKQPSRVLVVEPHQTLRTVLVHRLRQDGHLTAAVASAVEALDVCRDQSPDLLV
SAELLERSSAMRLSHQLRCPVIVLTARTGTEPMVGLLNEGADDVLRKPFGLEELAARCRTLLKRGHSGLQERVTVGPLEV
HLLLRQVTLRDKPVELSPREFALLCALLMPPGIVRSRQELLRMAWPPFSGGPRSVDTQILTLRRKLEQAGLGEGGGITTM
RQQGYRFSLDTLPDSES
>Mature_256_residues
TSIPRNLHSNEDTQVSSSGSLQMQTTPKQPSRVLVVEPHQTLRTVLVHRLRQDGHLTAAVASAVEALDVCRDQSPDLLVS
AELLERSSAMRLSHQLRCPVIVLTARTGTEPMVGLLNEGADDVLRKPFGLEELAARCRTLLKRGHSGLQERVTVGPLEVH
LLLRQVTLRDKPVELSPREFALLCALLMPPGIVRSRQELLRMAWPPFSGGPRSVDTQILTLRRKLEQAGLGEGGGITTMR
QQGYRFSLDTLPDSES

Specific function: Member of the two-component regulatory system sphR/sphS. Response regulator. Involved in inducible production of alkaline phosphatase in response to phosphate limitation as it is directly involved in the regulation of phoA transcription in response to pho

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790860, Length=224, Percent_Identity=35.7142857142857, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI1787375, Length=223, Percent_Identity=30.0448430493274, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI1786599, Length=225, Percent_Identity=31.1111111111111, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1788394, Length=226, Percent_Identity=30.0884955752212, Blast_Score=87, Evalue=8e-19,
Organism=Escherichia coli, GI1786911, Length=231, Percent_Identity=33.3333333333333, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1789809, Length=213, Percent_Identity=30.9859154929577, Blast_Score=77, Evalue=9e-16,
Organism=Escherichia coli, GI1790552, Length=148, Percent_Identity=35.8108108108108, Blast_Score=74, Evalue=7e-15,
Organism=Escherichia coli, GI1786784, Length=231, Percent_Identity=29.004329004329, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI2367329, Length=169, Percent_Identity=37.2781065088757, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI87082012, Length=220, Percent_Identity=27.7272727272727, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787229, Length=237, Percent_Identity=28.6919831223629, Blast_Score=64, Evalue=7e-12,
Organism=Escherichia coli, GI1789402, Length=131, Percent_Identity=37.4045801526718, Blast_Score=61, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 28354; Mature: 28223

Theoretical pI: Translated: 8.68; Mature: 8.68

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSIPRNLHSNEDTQVSSSGSLQMQTTPKQPSRVLVVEPHQTLRTVLVHRLRQDGHLTAA
CCCCCCCCCCCCCCEECCCCCEEEEECCCCCCEEEEECCHHHHHHHHHHHHHCCCCHHHH
VASAVEALDVCRDQSPDLLVSAELLERSSAMRLSHQLRCPVIVLTARTGTEPMVGLLNEG
HHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHCC
ADDVLRKPFGLEELAARCRTLLKRGHSGLQERVTVGPLEVHLLLRQVTLRDKPVELSPRE
HHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHHHCCCCCCCCHHH
FALLCALLMPPGIVRSRQELLRMAWPPFSGGPRSVDTQILTLRRKLEQAGLGEGGGITTM
HHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHH
RQQGYRFSLDTLPDSES
HHCCCEEEECCCCCCCC
>Mature Secondary Structure 
TSIPRNLHSNEDTQVSSSGSLQMQTTPKQPSRVLVVEPHQTLRTVLVHRLRQDGHLTAA
CCCCCCCCCCCCCEECCCCCEEEEECCCCCCEEEEECCHHHHHHHHHHHHHCCCCHHHH
VASAVEALDVCRDQSPDLLVSAELLERSSAMRLSHQLRCPVIVLTARTGTEPMVGLLNEG
HHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHCC
ADDVLRKPFGLEELAARCRTLLKRGHSGLQERVTVGPLEVHLLLRQVTLRDKPVELSPRE
HHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHHHCCCCCCCCHHH
FALLCALLMPPGIVRSRQELLRMAWPPFSGGPRSVDTQILTLRRKLEQAGLGEGGGITTM
HHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHH
RQQGYRFSLDTLPDSES
HHCCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8497200; 8157591 [H]