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The map label for this gene is sphS [H]

Identifier: 33863268

GI number: 33863268

Start: 1074070

End: 1074477

Strand: Direct

Name: sphS [H]

Synonym: PMT0997

Alternate gene names: 33863268

Gene position: 1074070-1074477 (Clockwise)

Preceding gene: 33863267

Following gene: 33863272

Centisome position: 44.55

GC content: 41.42

Gene sequence:

>408_bases
ATGGCGGAAATACGTAGTATAAGCCTGCTAACATATATTGACGAACCTGGCCTCATACTTGGTGATAAGCAACGACTACA
AACAGCATTGATTAATTTGCATGATAACGCTCTGCTTTGCTCACCTAAGAATGGCTCTATTGAAGTAGATATCCTTCCAA
AGGGGCTTTGGTGGCTTATCAAGATCCGTGATCATGGCCCTGGTCTTAGTGAGATTGTCTTAAGCAATATGTTTCAGCGT
TTTTATCGAGGAGATATCTCAAGAAAACGAACAAGCCGAAGTGGCAGTGGTCTTGGATTAGCCATTGTCGAGCAAATTAT
TATCAGTCATGGCCGAAGCATCAAAGCAGAAAATCACCCTGAAGGAGGAACTAGTATCGAACTTTTACTACCTAAAGGCA
AATACTAA

Upstream 100 bases:

>100_bases
AGCTATGAAGGCTTAAGAATATACAGCAAATAGGTATTCAAGTTACATTGCCAATTCTGTAGAAGATCTCCTGGAAATTT
ACAGGAATACCATTCGCCCT

Downstream 100 bases:

>100_bases
GAAATAATCGAAAAATAAAAAAAATACTCGTAGCCAAGATGCATTGAGAATATGCTATTTGAAAATAAAAAAATGCCCTC
CGTTTAAGGAGGGCATTGAA

Product: two component sensor histidine kinase fragment, pseudogene

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 135; Mature: 134

Protein sequence:

>135_residues
MAEIRSISLLTYIDEPGLILGDKQRLQTALINLHDNALLCSPKNGSIEVDILPKGLWWLIKIRDHGPGLSEIVLSNMFQR
FYRGDISRKRTSRSGSGLGLAIVEQIIISHGRSIKAENHPEGGTSIELLLPKGKY

Sequences:

>Translated_135_residues
MAEIRSISLLTYIDEPGLILGDKQRLQTALINLHDNALLCSPKNGSIEVDILPKGLWWLIKIRDHGPGLSEIVLSNMFQR
FYRGDISRKRTSRSGSGLGLAIVEQIIISHGRSIKAENHPEGGTSIELLLPKGKY
>Mature_134_residues
AEIRSISLLTYIDEPGLILGDKQRLQTALINLHDNALLCSPKNGSIEVDILPKGLWWLIKIRDHGPGLSEIVLSNMFQRF
YRGDISRKRTSRSGSGLGLAIVEQIIISHGRSIKAENHPEGGTSIELLLPKGKY

Specific function: Member of the two-component regulatory system sphR/sphS. Sensory kinase. Is involved in inducible production of alkaline phosphatase in response to phosphate limitation as it is directly involved in the regulation of phoA transcription in response to phos

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1788393, Length=127, Percent_Identity=35.4330708661417, Blast_Score=74, Evalue=4e-15,
Organism=Escherichia coli, GI1790346, Length=116, Percent_Identity=37.9310344827586, Blast_Score=66, Evalue=1e-12,
Organism=Escherichia coli, GI1786912, Length=115, Percent_Identity=35.6521739130435, Blast_Score=64, Evalue=3e-12,
Organism=Escherichia coli, GI87081816, Length=129, Percent_Identity=34.1085271317829, Blast_Score=59, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 14971; Mature: 14839

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEIRSISLLTYIDEPGLILGDKQRLQTALINLHDNALLCSPKNGSIEVDILPKGLWWLI
CCCCCEEEEEEEECCCCEEECCHHHHHHHHEEECCCEEEECCCCCCEEEEEECCCEEEEE
KIRDHGPGLSEIVLSNMFQRFYRGDISRKRTSRSGSGLGLAIVEQIIISHGRSIKAENHP
EEECCCCCHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCC
EGGTSIELLLPKGKY
CCCCEEEEEECCCCC
>Mature Secondary Structure 
AEIRSISLLTYIDEPGLILGDKQRLQTALINLHDNALLCSPKNGSIEVDILPKGLWWLI
CCCCEEEEEEEECCCCEEECCHHHHHHHHEEECCCEEEECCCCCCEEEEEECCCEEEEE
KIRDHGPGLSEIVLSNMFQRFYRGDISRKRTSRSGSGLGLAIVEQIIISHGRSIKAENHP
EEECCCCCHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCC
EGGTSIELLLPKGKY
CCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8497200 [H]