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The map label for this gene is phoB [H]

Identifier: 33863265

GI number: 33863265

Start: 1072591

End: 1073328

Strand: Direct

Name: phoB [H]

Synonym: PMT0994

Alternate gene names: 33863265

Gene position: 1072591-1073328 (Clockwise)

Preceding gene: 33863264

Following gene: 33863266

Centisome position: 44.49

GC content: 40.65

Gene sequence:

>738_bases
ATGACTGTTGCACCTCCAAAGGTCATACCTCCTCAGTCATTGAAGGTTTTCCGATTGCTTGTTGTTGAAGACGATGACGC
GATTCGAGAAACTATTGGCGAGGCTTTGTGTGCTGAGGGTTTTGAAACTATATTGTGTGGAGATGGATTCGAAGCTGTTT
CGATCCTTACAAGTCAACCGAGACATAAATTTGATCTAATTATTTTAGATGTGATGCTCCCTGGCATTGGTGGTCTAGAT
ATTTGTCGAAGATTGAGAGAATTTGATCCTAAAACACGCATATTCATGATCAGTGCTCGAGATAATGAAATCGATCGTGT
TTTGGGGCTTGAGGTTGGAGCTGATGATTACCTAGTTAAACCATTTAGCGTTAGAGAACTTGTTGCACGTTGTCGAGCAC
AATTACGTCGTTATCAGGAACGATCTGACGATTCAAATGCAAAAATGATTGAATATGGGAATTTATGTTTATACATTGAA
GAATGTAGAGTCACCAAGGATGGAAAAGATCTAAGCCTTGCTCCAAAGGAGTATAAAATATTAAAGTTATTACTAGAACA
CCCAAGACAGGTCCTGAGCCGAGATCAGATTCTTGAGCGAATATGGGGTGATGATTATTTTGGCGATACCAAAACTGTAG
ACGTACACATTCGGTGGATAAGAGAAAAGATTGAGCAGGACCCTTCATCCCCTCAGTGCATCCATACTGTGAGAGGATTT
GGTTATCGCTTTGGGTAA

Upstream 100 bases:

>100_bases
TGAGTGATGAGGCTCAGGCGCAAGCTGATGGTTTGGGCTTTGTCCCCCTTCAGGGTGAAATTCGCTCCAAGGCTCGAGCA
GCAGTTGAAAAGGTCGGCAA

Downstream 100 bases:

>100_bases
TATTGATCTTGTATTTTTCTCTGATTTATCTTAGGATTCTCAATTGGTATCGCCACAAGGCAAAACTTGCCAAGGAAATT
ACTTAAAGAAAAATACATCT

Product: two-component response regulator, phosphate

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 245; Mature: 244

Protein sequence:

>245_residues
MTVAPPKVIPPQSLKVFRLLVVEDDDAIRETIGEALCAEGFETILCGDGFEAVSILTSQPRHKFDLIILDVMLPGIGGLD
ICRRLREFDPKTRIFMISARDNEIDRVLGLEVGADDYLVKPFSVRELVARCRAQLRRYQERSDDSNAKMIEYGNLCLYIE
ECRVTKDGKDLSLAPKEYKILKLLLEHPRQVLSRDQILERIWGDDYFGDTKTVDVHIRWIREKIEQDPSSPQCIHTVRGF
GYRFG

Sequences:

>Translated_245_residues
MTVAPPKVIPPQSLKVFRLLVVEDDDAIRETIGEALCAEGFETILCGDGFEAVSILTSQPRHKFDLIILDVMLPGIGGLD
ICRRLREFDPKTRIFMISARDNEIDRVLGLEVGADDYLVKPFSVRELVARCRAQLRRYQERSDDSNAKMIEYGNLCLYIE
ECRVTKDGKDLSLAPKEYKILKLLLEHPRQVLSRDQILERIWGDDYFGDTKTVDVHIRWIREKIEQDPSSPQCIHTVRGF
GYRFG
>Mature_244_residues
TVAPPKVIPPQSLKVFRLLVVEDDDAIRETIGEALCAEGFETILCGDGFEAVSILTSQPRHKFDLIILDVMLPGIGGLDI
CRRLREFDPKTRIFMISARDNEIDRVLGLEVGADDYLVKPFSVRELVARCRAQLRRYQERSDDSNAKMIEYGNLCLYIEE
CRVTKDGKDLSLAPKEYKILKLLLEHPRQVLSRDQILERIWGDDYFGDTKTVDVHIRWIREKIEQDPSSPQCIHTVRGFG
YRFG

Specific function: Member of the two-component regulatory system sphR/sphS. Response regulator. Involved in inducible production of alkaline phosphatase in response to phosphate limitation as it is directly involved in the regulation of phoA transcription in response to pho

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789809, Length=231, Percent_Identity=37.6623376623377, Blast_Score=165, Evalue=2e-42,
Organism=Escherichia coli, GI1786599, Length=231, Percent_Identity=41.1255411255411, Blast_Score=154, Evalue=6e-39,
Organism=Escherichia coli, GI1786784, Length=226, Percent_Identity=36.7256637168142, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI87082012, Length=228, Percent_Identity=36.4035087719298, Blast_Score=140, Evalue=7e-35,
Organism=Escherichia coli, GI1788394, Length=232, Percent_Identity=36.2068965517241, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1790863, Length=236, Percent_Identity=34.3220338983051, Blast_Score=137, Evalue=5e-34,
Organism=Escherichia coli, GI1790860, Length=222, Percent_Identity=36.9369369369369, Blast_Score=137, Evalue=8e-34,
Organism=Escherichia coli, GI1789402, Length=229, Percent_Identity=35.8078602620087, Blast_Score=130, Evalue=9e-32,
Organism=Escherichia coli, GI2367329, Length=229, Percent_Identity=37.5545851528384, Blast_Score=130, Evalue=9e-32,
Organism=Escherichia coli, GI1786911, Length=229, Percent_Identity=32.7510917030568, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1790552, Length=226, Percent_Identity=34.5132743362832, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1787229, Length=231, Percent_Identity=31.6017316017316, Blast_Score=107, Evalue=8e-25,
Organism=Escherichia coli, GI1787375, Length=229, Percent_Identity=30.5676855895196, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI145693140, Length=236, Percent_Identity=30.0847457627119, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1790299, Length=129, Percent_Identity=28.6821705426357, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1788550, Length=113, Percent_Identity=28.3185840707965, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787487, Length=118, Percent_Identity=30.5084745762712, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 28143; Mature: 28012

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVAPPKVIPPQSLKVFRLLVVEDDDAIRETIGEALCAEGFETILCGDGFEAVSILTSQP
CCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHCCC
RHKFDLIILDVMLPGIGGLDICRRLREFDPKTRIFMISARDNEIDRVLGLEVGADDYLVK
CCHHHHEEEEHHCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHEEEECCCCCEEC
PFSVRELVARCRAQLRRYQERSDDSNAKMIEYGNLCLYIEECRVTKDGKDLSLAPKEYKI
CCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCEEEEEEECEECCCCCCCCCCCHHHHH
LKLLLEHPRQVLSRDQILERIWGDDYFGDTKTVDVHIRWIREKIEQDPSSPQCIHTVRGF
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHCCCCCCHHHHHHHHC
GYRFG
CCCCC
>Mature Secondary Structure 
TVAPPKVIPPQSLKVFRLLVVEDDDAIRETIGEALCAEGFETILCGDGFEAVSILTSQP
CCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHCCC
RHKFDLIILDVMLPGIGGLDICRRLREFDPKTRIFMISARDNEIDRVLGLEVGADDYLVK
CCHHHHEEEEHHCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHEEEECCCCCEEC
PFSVRELVARCRAQLRRYQERSDDSNAKMIEYGNLCLYIEECRVTKDGKDLSLAPKEYKI
CCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCEEEEEEECEECCCCCCCCCCCHHHHH
LKLLLEHPRQVLSRDQILERIWGDDYFGDTKTVDVHIRWIREKIEQDPSSPQCIHTVRGF
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHCCCCCCHHHHHHHHC
GYRFG
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8497200; 8157591 [H]