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The map label for this gene is ndhF [H]

Identifier: 33863120

GI number: 33863120

Start: 927194

End: 928099

Strand: Reverse

Name: ndhF [H]

Synonym: PMT0848

Alternate gene names: 33863120

Gene position: 928099-927194 (Counterclockwise)

Preceding gene: 33863121

Following gene: 33863117

Centisome position: 38.5

GC content: 43.82

Gene sequence:

>906_bases
ATGCCTTTACCTTCAGAACTTGCTTGGTTAATTCCAGTGCTGCCTTTGGCAGGTGCATGCTTTGTAGGATTTTTGTTGAT
CAGTTTCAAGCTCACCATGAACCGACTGAGTAAGCCGGTTTCATTCCTGTTAGTTAGCTGTGTTGGTGCAGCAGCTGTAC
TCAGTTACGCCCTTCTTGCGGAACAAAGGGCTGGAACTGTTGCCAGTAAGGTGATCTTCGACTTGCCAGGCTTAACTGAT
CTCAATTTACAGATTGGTTTTGTTGTAGATGGCATTGGTACCGAGATGCTTGCACTGATTAGCACTGCAGGAATATTGTT
GATGTTGTCCGCACATATTTATATGGTGGGCAAAAAAAACTATGTGAGTTTTTTTACTTATCTAGGCTTTTTTACTAGTG
CCTTATTAGGTCTAGCCCTAAGTCCAAACCTATTAGAGATGTTTTTCTTTTGGCTGCTGGTTGGGATCTCTTCATACCTT
CTAGTTGGTTTTTGGTACGACACCAATGGCTCTTCCAAAACAACCCAAAAGATTTTCCTGGTAGACCGTGTTGGTGACGT
TGGATTTCTACTTGGTAGTCTTGGTTTGTTCTGGGTGACCAGAAGTTTTGGATTTGATGAAAGTGGCCCCCTTCTCGAAG
AAGCGATTATCAGCGGTAAGCTTTCAAATCCAACGACTCTTCTCCTTTGCTTCCTTATTATTATGGGACCAATTGCAAAA
CTAATAGAGTTTCCATTGTATATCTGGCGGCCTGATGTGTTGAAAACACCCGTTCCTGCCTCGGCTCTCATTCATGCAAC
AACCCTAGTTGCTGCAGGTGTTTTTGTCGTTGCCAGGCTTGAACCAGTTTTGGCTGCTGGAGCTAGGGCCTTGCCTGGAA
AGGTTTTGGCCAATTTATTTGCTTGA

Upstream 100 bases:

>100_bases
TCTCCAATTTCCATGTTTGGTGTCTTCGGCTTAATTTTCTTGGTTGGACTTGACGACCTGACAAAGGAACTTGCAACTTC
TAACTTCACTAAATTCGCTA

Downstream 100 bases:

>100_bases
CTCTTTATATTTAGTTTGCAGGGAGAGAGTATTGATAGTGATTAAGGTTATCCGTAAGAAAGATGATCAAGTCTTTAAGA
TCGAGGTTAGAGATAAGTAG

Product: NADH dehydrogenase (complex I) subunit

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase I, chain 5; NDH-1, chain 5 [H]

Number of amino acids: Translated: 301; Mature: 300

Protein sequence:

>301_residues
MPLPSELAWLIPVLPLAGACFVGFLLISFKLTMNRLSKPVSFLLVSCVGAAAVLSYALLAEQRAGTVASKVIFDLPGLTD
LNLQIGFVVDGIGTEMLALISTAGILLMLSAHIYMVGKKNYVSFFTYLGFFTSALLGLALSPNLLEMFFFWLLVGISSYL
LVGFWYDTNGSSKTTQKIFLVDRVGDVGFLLGSLGLFWVTRSFGFDESGPLLEEAIISGKLSNPTTLLLCFLIIMGPIAK
LIEFPLYIWRPDVLKTPVPASALIHATTLVAAGVFVVARLEPVLAAGARALPGKVLANLFA

Sequences:

>Translated_301_residues
MPLPSELAWLIPVLPLAGACFVGFLLISFKLTMNRLSKPVSFLLVSCVGAAAVLSYALLAEQRAGTVASKVIFDLPGLTD
LNLQIGFVVDGIGTEMLALISTAGILLMLSAHIYMVGKKNYVSFFTYLGFFTSALLGLALSPNLLEMFFFWLLVGISSYL
LVGFWYDTNGSSKTTQKIFLVDRVGDVGFLLGSLGLFWVTRSFGFDESGPLLEEAIISGKLSNPTTLLLCFLIIMGPIAK
LIEFPLYIWRPDVLKTPVPASALIHATTLVAAGVFVVARLEPVLAAGARALPGKVLANLFA
>Mature_300_residues
PLPSELAWLIPVLPLAGACFVGFLLISFKLTMNRLSKPVSFLLVSCVGAAAVLSYALLAEQRAGTVASKVIFDLPGLTDL
NLQIGFVVDGIGTEMLALISTAGILLMLSAHIYMVGKKNYVSFFTYLGFFTSALLGLALSPNLLEMFFFWLLVGISSYLL
VGFWYDTNGSSKTTQKIFLVDRVGDVGFLLGSLGLFWVTRSFGFDESGPLLEEAIISGKLSNPTTLLLCFLIIMGPIAKL
IEFPLYIWRPDVLKTPVPASALIHATTLVAAGVFVVARLEPVLAAGARALPGKVLANLFA

Specific function: NDH-1 shuttles electrons from NAD(P)H, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton tran

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=171, Percent_Identity=32.1637426900585, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1788614, Length=272, Percent_Identity=32.3529411764706, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI1788829, Length=265, Percent_Identity=24.9056603773585, Blast_Score=75, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR001516
- InterPro:   IPR002128
- InterPro:   IPR003945
- InterPro:   IPR018393 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF01010 Oxidored_q1_C; PF00662 Oxidored_q1_N [H]

EC number: 1.6.5.3

Molecular weight: Translated: 32331; Mature: 32200

Theoretical pI: Translated: 8.03; Mature: 8.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLPSELAWLIPVLPLAGACFVGFLLISFKLTMNRLSKPVSFLLVSCVGAAAVLSYALLA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EQRAGTVASKVIFDLPGLTDLNLQIGFVVDGIGTEMLALISTAGILLMLSAHIYMVGKKN
HHHHHHHHHHHHHHCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHEEECCCH
YVSFFTYLGFFTSALLGLALSPNLLEMFFFWLLVGISSYLLVGFWYDTNGSSKTTQKIFL
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCHHHEEEE
VDRVGDVGFLLGSLGLFWVTRSFGFDESGPLLEEAIISGKLSNPTTLLLCFLIIMGPIAK
EECCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
LIEFPLYIWRPDVLKTPVPASALIHATTLVAAGVFVVARLEPVLAAGARALPGKVLANLF
HHHCCHHEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHC
A
C
>Mature Secondary Structure 
PLPSELAWLIPVLPLAGACFVGFLLISFKLTMNRLSKPVSFLLVSCVGAAAVLSYALLA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EQRAGTVASKVIFDLPGLTDLNLQIGFVVDGIGTEMLALISTAGILLMLSAHIYMVGKKN
HHHHHHHHHHHHHHCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHEEECCCH
YVSFFTYLGFFTSALLGLALSPNLLEMFFFWLLVGISSYLLVGFWYDTNGSSKTTQKIFL
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCHHHEEEE
VDRVGDVGFLLGSLGLFWVTRSFGFDESGPLLEEAIISGKLSNPTTLLLCFLIIMGPIAK
EECCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
LIEFPLYIWRPDVLKTPVPASALIHATTLVAAGVFVVARLEPVLAAGARALPGKVLANLF
HHHCCHHEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHC
A
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]