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The map label for this gene is rsmE [H]

Identifier: 33863058

GI number: 33863058

Start: 860951

End: 861727

Strand: Reverse

Name: rsmE [H]

Synonym: PMT0786

Alternate gene names: 33863058

Gene position: 861727-860951 (Counterclockwise)

Preceding gene: 33863059

Following gene: 33863057

Centisome position: 35.74

GC content: 51.48

Gene sequence:

>777_bases
TTGGCTGAGCTCAGGCGCCTTTTGGTTGAATCGCAGCGGTTTGATGCAATCACTGCTTCTGACCATTTGTTATCTCTCAA
GCAAGACGAAGTCCATTACCTGCGCAGAGTGCTTCGCCTTCGCAATGGGCAAGCGGTTGCGCTTGTTGATGGTGTTGGCC
ATTTCTGGGAGGCCCATCTACAAGCAGGTGATTTTTTAAAACTTGACACGTCACCTTCGTCTCCTTCTCTACAACAACCT
CGCCATCGTCAACAACTCGGTTTGGCTGTTGTTCTCCCAAAGCGTGGCTTTGAGGAGGTTTTGAGGATGAGTTGTGAACT
AGGCGTCAACCTGATTCAGCCTCTACGTTCTGATAGGTGTACTCCTCAGGCGGAGGAGCGACCATTGCGATGGAGGGTGA
TCCTTAGGGAAGCAGTTGAGCAATCTGAGCAGCTTTGGCAGCCAGAACTTTTGCCGATAGCAGAAGCTTCAGCCTTGTGG
AAAAGTCCACCAGATCATGCAGTTTTTGCTTTGGCCACCACTCGTCGGCAGGGACTGATTGATCTACAACTTTTGATGGA
AGGACTGACCCCAGAAATCAACCAAGTCACAGTTGCGATTGGTCCTGAGGGTGGTTGGAGCCCACTTGAAGAGTGTGCCG
CTGAGGCCGCCGGTTGGGTGCCTGTGGCGTTGGGAGATTCAATCCTGCGCACTTGCACAGCTGCTGTGGCAGCAGCTCAA
ACAATGGTTTCAAGTCGACGGATCAGCTTGTCAAGAGATCAGTCAAGATCTGATTGA

Upstream 100 bases:

>100_bases
TGGTGCAGTTGGATGAAGTGTTGATTGGTGGTGTCAATGATGACTGGCCTGTTGAGCCCCATCCCGACAGCATCTTCCCT
GTGGAGGCCTAGGCAGTCTC

Downstream 100 bases:

>100_bases
ATTCCTGCAGGCTTGTTTTTGATCTAAGCGGCTGCATTTTTACTCACGAATGTCAGCGCTTATCGGCGTCTTTTTCAAGC
AAACCTTCATTTCATAGCGG

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MAELRRLLVESQRFDAITASDHLLSLKQDEVHYLRRVLRLRNGQAVALVDGVGHFWEAHLQAGDFLKLDTSPSSPSLQQP
RHRQQLGLAVVLPKRGFEEVLRMSCELGVNLIQPLRSDRCTPQAEERPLRWRVILREAVEQSEQLWQPELLPIAEASALW
KSPPDHAVFALATTRRQGLIDLQLLMEGLTPEINQVTVAIGPEGGWSPLEECAAEAAGWVPVALGDSILRTCTAAVAAAQ
TMVSSRRISLSRDQSRSD

Sequences:

>Translated_258_residues
MAELRRLLVESQRFDAITASDHLLSLKQDEVHYLRRVLRLRNGQAVALVDGVGHFWEAHLQAGDFLKLDTSPSSPSLQQP
RHRQQLGLAVVLPKRGFEEVLRMSCELGVNLIQPLRSDRCTPQAEERPLRWRVILREAVEQSEQLWQPELLPIAEASALW
KSPPDHAVFALATTRRQGLIDLQLLMEGLTPEINQVTVAIGPEGGWSPLEECAAEAAGWVPVALGDSILRTCTAAVAAAQ
TMVSSRRISLSRDQSRSD
>Mature_257_residues
AELRRLLVESQRFDAITASDHLLSLKQDEVHYLRRVLRLRNGQAVALVDGVGHFWEAHLQAGDFLKLDTSPSSPSLQQPR
HRQQLGLAVVLPKRGFEEVLRMSCELGVNLIQPLRSDRCTPQAEERPLRWRVILREAVEQSEQLWQPELLPIAEASALWK
SPPDHAVFALATTRRQGLIDLQLLMEGLTPEINQVTVAIGPEGGWSPLEECAAEAAGWVPVALGDSILRTCTAAVAAAQT
MVSSRRISLSRDQSRSD

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=230, Percent_Identity=26.9565217391304, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001206
- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 28684; Mature: 28553

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAELRRLLVESQRFDAITASDHLLSLKQDEVHYLRRVLRLRNGQAVALVDGVGHFWEAHL
CHHHHHHHHHHHHCCEEECCHHHHHCCHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHH
QAGDFLKLDTSPSSPSLQQPRHRQQLGLAVVLPKRGFEEVLRMSCELGVNLIQPLRSDRC
CCCCEEEECCCCCCCCCCCCHHHHHCCEEEEECCCCHHHHHHHHHHHCHHHHHHHHCCCC
TPQAEERPLRWRVILREAVEQSEQLWQPELLPIAEASALWKSPPDHAVFALATTRRQGLI
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCEEEEEEHHCCCCCH
DLQLLMEGLTPEINQVTVAIGPEGGWSPLEECAAEAAGWVPVALGDSILRTCTAAVAAAQ
HHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHH
TMVSSRRISLSRDQSRSD
HHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
AELRRLLVESQRFDAITASDHLLSLKQDEVHYLRRVLRLRNGQAVALVDGVGHFWEAHL
HHHHHHHHHHHHCCEEECCHHHHHCCHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHH
QAGDFLKLDTSPSSPSLQQPRHRQQLGLAVVLPKRGFEEVLRMSCELGVNLIQPLRSDRC
CCCCEEEECCCCCCCCCCCCHHHHHCCEEEEECCCCHHHHHHHHHHHCHHHHHHHHCCCC
TPQAEERPLRWRVILREAVEQSEQLWQPELLPIAEASALWKSPPDHAVFALATTRRQGLI
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCEEEEEEHHCCCCCH
DLQLLMEGLTPEINQVTVAIGPEGGWSPLEECAAEAAGWVPVALGDSILRTCTAAVAAAQ
HHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHH
TMVSSRRISLSRDQSRSD
HHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]