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The map label for this gene is leuS [H]

Identifier: 33862987

GI number: 33862987

Start: 772530

End: 775166

Strand: Reverse

Name: leuS [H]

Synonym: PMT0715

Alternate gene names: 33862987

Gene position: 775166-772530 (Counterclockwise)

Preceding gene: 33862993

Following gene: 33862985

Centisome position: 32.15

GC content: 48.08

Gene sequence:

>2637_bases
GTGACGGAGTCTTTCCCCTCAACGTCTGCCTCCAGCCAATCCTCAGCTCGATATGACCCTATTGAGCTTGAAACGCGTTG
GCAAAAGGAGTGGTTACGACAAGGATTAGATCGAACGCCAGTCGCAGAAACTAACCAGAAACGCTTTTACGCCCTATCGA
TGTTCCCTTATCCATCAGGGAAACTTCACATGGGCCACGTACGCAATTACGTGATTACCGATGTGATTGCCAGGGTGCAG
CGCATGCGAGGTGATGCGGTACTTCACCCAATGGGATGGGATGCCTTTGGCTTACCAGCTGAGAATGCAGCCATCGCCAG
AAACGTAGATCCAGGTGATTGGACAGACCAAAATATTGCCCAAATGAGGGCCCAGCTTGATCGACTGGGTTTGTCGATTG
ACTGGGACCGGCAACAGGCGACTTGCCATCAGGACTATTACCGCTGGACCCAGTGGTTGTTCCTTGAACTTTTTGCGGGT
GGATTGGCTTATCAGAAGGAAGCGACAGTCAACTGGGATCCCGTCGACAAAACTGTTCTGGCTAATGAACAAGTCGATGG
CGAGGGTCGTTCATGGCGGTCAGGCGCTCTTGTGGAACAGCGACAACTCAAGCAATGGTTCCTGCGAATCACCGACTATG
CCGATGCGTTAATTGATGACCTTGATGAATTGACTGGTTGGCCCGAGCGTGTCCGCACGATGCAAGCCAATTGGATTGGT
CGCTCTCATGGTGCTGAGATCAAGTTCAGAGTCGCTGGTGTAGCCAACTCAATCATCACGGTCTTCACAACAAGGCCAGA
CACACTTCACGGTGCAAGTTATGTGGTTTTGGCACCTGAGCACCCTTTGGTGGAATCCCTAACAAGTCCGGAACAACGTC
TTGCCGTTACTGCATTCTGTGACCTTATAAGCCAACTCAGCGTCAAGGATCGCACCGCTGAGGACCAACCAAAACGTGGG
GTCCCGATCGGTGCACAGGTGATCAATCCAGTCAATGGTGAATCACTACCCGTTTGGATTGCCGATTACGTATTGGCTGA
CTATGGCTCAGGAGCCGTTATGGGAGTACCCGCTCATGATGAGCGGGATTTCATCTTTGCTCGCAGCCACGAACTACCGA
TACGCATTGTGGTGCAGCTGCCAGATACAGACGAGCATCACAATGATGGACAAGCCTGGACCGGGGCCGGAGTTCTTGTA
AATTCTGGAGCCTTTGATGGTCTCTCCACAGAGGAAGCGAAGGTTGCGATTACAACCCATGGAGCTTCTGAGGGTTGGGC
TCAAAGCAAAGTGCAATACCGTTTGCGCGATTGGCTCATTTCTCGTCAGCGCTACTGGGGTTGCCCGATCCCAATCATTC
ACTGTGCAAGCTGTGGAATAGTTCCTGTTCCGCAAGAGGACCTACCTGTAACCCTTCCTAGGGACATAGATCTTTCAGGA
AAAGGAGGATCCCCCATAGCGCAAGAACAGGCTTGGGTTGAGGTTAAATGTCCTATTTGTGGTGAAAAAGCTCACAGGGA
AACAGACACGATGGACACCTTTATGTGTTCTTCATGGTATTACCTACGTTTTGCTGATCCACTCAATAGTCAACGTCCAT
TTGATAAAGATATTGTTGATGAATGGTTGCCTGTTGATCAGTATGTAGGTGGTATTGAACATGCAATCCTCCATCTCCTA
TATGCACGCTTTTTTACAAAAGCACTCCACGATAGAAACCTCATTGGCTTTAAAGAACCATTTAATCGATTACTTACTCA
AGGCATGGTTCAAGGGCTTACCTACCGCAATGCTAAAAATGGCAGTTATATTTCTCCTGAACTTGTTAGTGATGATAGTG
ATCCGAGGGACCCAGAGTCTGGAGACAGACTAGAGGTCCTGTTTGAAAAGATGTCTAAATCAAAATATAATGGCGTTGAT
CCTGCCGTTGTAATCGATCGATACGGTGCCGACACCGCTAGAATGTTCATACTTTTTAAAGCGCCACCAGAGAAAGACCT
GGAATGGGATGATGCAGATGTTGAAGGTCAATTTCGTTTTCTGCAACGACTTATTCGTCTGATTGATAGTTTTGCATGGC
CTAAGACTGATGGTGAGAATGCCTCCATTTCTTCGGCAAACTTGATCATAGATTCAGCTGATCTCAGTGAAGAAGAGATC
AACATGAGAAGAGCTACTCACAAGGCGATAGAGGCAATAACTGAGGACTTAAGTGGTGACATTCAATTGAATACAGCCAT
CTCCGAACTCATGAAGCTATCCAACTCGTTGAGTGGAAAACTAGACAAGGTACGTAATGAAGTGGCTGCAGAGGCTCTTT
CAGTTTTAGTTCGATTGATGGCACCCTTTGCTCCTCATCTTGCAGAAGAATTCTGGCTGAAGCTTCATGGGCATGGAAGC
ATCCATCAACAATCCTGGCCTGTAATCGACCCAAGCGCACTTGTTCTCGAAACGATTGAGCTCGTCATTCAAGTCAAAGG
GAAGGTACGTGGAAAAATCCAGGTACCTGCTAACGCAGACAAAAAGACATTAGAAGAATTGGCCTTAAACAGTGACATCG
CCGTCAAATGGTTAGAAGGTCAATCGCCTAGACGGATCATCATTGTGCCAGGCAAGTTGGTGAACCTAGTGCCATAG

Upstream 100 bases:

>100_bases
GATTAACCGTCGGGCTGTACTGATAATTCGTCCACTGGGCTAACTCAACTCCCTAGCGTCATCTTGTTATGGCACGATCG
AAAGTTGGTGAAACTTGTAC

Downstream 100 bases:

>100_bases
AAATGATCAGCCTTTGCTGAAACGTAGTGAAGTCGGAATTCCCCAATCACCTTCAGCAACATAACCACGCTTATTCGCAG
TTAGATGCCTCATGATCCAA

Product: leucyl-tRNA synthetase

Products: NA

Alternate protein names: Leucine--tRNA ligase; LeuRS [H]

Number of amino acids: Translated: 878; Mature: 877

Protein sequence:

>878_residues
MTESFPSTSASSQSSARYDPIELETRWQKEWLRQGLDRTPVAETNQKRFYALSMFPYPSGKLHMGHVRNYVITDVIARVQ
RMRGDAVLHPMGWDAFGLPAENAAIARNVDPGDWTDQNIAQMRAQLDRLGLSIDWDRQQATCHQDYYRWTQWLFLELFAG
GLAYQKEATVNWDPVDKTVLANEQVDGEGRSWRSGALVEQRQLKQWFLRITDYADALIDDLDELTGWPERVRTMQANWIG
RSHGAEIKFRVAGVANSIITVFTTRPDTLHGASYVVLAPEHPLVESLTSPEQRLAVTAFCDLISQLSVKDRTAEDQPKRG
VPIGAQVINPVNGESLPVWIADYVLADYGSGAVMGVPAHDERDFIFARSHELPIRIVVQLPDTDEHHNDGQAWTGAGVLV
NSGAFDGLSTEEAKVAITTHGASEGWAQSKVQYRLRDWLISRQRYWGCPIPIIHCASCGIVPVPQEDLPVTLPRDIDLSG
KGGSPIAQEQAWVEVKCPICGEKAHRETDTMDTFMCSSWYYLRFADPLNSQRPFDKDIVDEWLPVDQYVGGIEHAILHLL
YARFFTKALHDRNLIGFKEPFNRLLTQGMVQGLTYRNAKNGSYISPELVSDDSDPRDPESGDRLEVLFEKMSKSKYNGVD
PAVVIDRYGADTARMFILFKAPPEKDLEWDDADVEGQFRFLQRLIRLIDSFAWPKTDGENASISSANLIIDSADLSEEEI
NMRRATHKAIEAITEDLSGDIQLNTAISELMKLSNSLSGKLDKVRNEVAAEALSVLVRLMAPFAPHLAEEFWLKLHGHGS
IHQQSWPVIDPSALVLETIELVIQVKGKVRGKIQVPANADKKTLEELALNSDIAVKWLEGQSPRRIIIVPGKLVNLVP

Sequences:

>Translated_878_residues
MTESFPSTSASSQSSARYDPIELETRWQKEWLRQGLDRTPVAETNQKRFYALSMFPYPSGKLHMGHVRNYVITDVIARVQ
RMRGDAVLHPMGWDAFGLPAENAAIARNVDPGDWTDQNIAQMRAQLDRLGLSIDWDRQQATCHQDYYRWTQWLFLELFAG
GLAYQKEATVNWDPVDKTVLANEQVDGEGRSWRSGALVEQRQLKQWFLRITDYADALIDDLDELTGWPERVRTMQANWIG
RSHGAEIKFRVAGVANSIITVFTTRPDTLHGASYVVLAPEHPLVESLTSPEQRLAVTAFCDLISQLSVKDRTAEDQPKRG
VPIGAQVINPVNGESLPVWIADYVLADYGSGAVMGVPAHDERDFIFARSHELPIRIVVQLPDTDEHHNDGQAWTGAGVLV
NSGAFDGLSTEEAKVAITTHGASEGWAQSKVQYRLRDWLISRQRYWGCPIPIIHCASCGIVPVPQEDLPVTLPRDIDLSG
KGGSPIAQEQAWVEVKCPICGEKAHRETDTMDTFMCSSWYYLRFADPLNSQRPFDKDIVDEWLPVDQYVGGIEHAILHLL
YARFFTKALHDRNLIGFKEPFNRLLTQGMVQGLTYRNAKNGSYISPELVSDDSDPRDPESGDRLEVLFEKMSKSKYNGVD
PAVVIDRYGADTARMFILFKAPPEKDLEWDDADVEGQFRFLQRLIRLIDSFAWPKTDGENASISSANLIIDSADLSEEEI
NMRRATHKAIEAITEDLSGDIQLNTAISELMKLSNSLSGKLDKVRNEVAAEALSVLVRLMAPFAPHLAEEFWLKLHGHGS
IHQQSWPVIDPSALVLETIELVIQVKGKVRGKIQVPANADKKTLEELALNSDIAVKWLEGQSPRRIIIVPGKLVNLVP
>Mature_877_residues
TESFPSTSASSQSSARYDPIELETRWQKEWLRQGLDRTPVAETNQKRFYALSMFPYPSGKLHMGHVRNYVITDVIARVQR
MRGDAVLHPMGWDAFGLPAENAAIARNVDPGDWTDQNIAQMRAQLDRLGLSIDWDRQQATCHQDYYRWTQWLFLELFAGG
LAYQKEATVNWDPVDKTVLANEQVDGEGRSWRSGALVEQRQLKQWFLRITDYADALIDDLDELTGWPERVRTMQANWIGR
SHGAEIKFRVAGVANSIITVFTTRPDTLHGASYVVLAPEHPLVESLTSPEQRLAVTAFCDLISQLSVKDRTAEDQPKRGV
PIGAQVINPVNGESLPVWIADYVLADYGSGAVMGVPAHDERDFIFARSHELPIRIVVQLPDTDEHHNDGQAWTGAGVLVN
SGAFDGLSTEEAKVAITTHGASEGWAQSKVQYRLRDWLISRQRYWGCPIPIIHCASCGIVPVPQEDLPVTLPRDIDLSGK
GGSPIAQEQAWVEVKCPICGEKAHRETDTMDTFMCSSWYYLRFADPLNSQRPFDKDIVDEWLPVDQYVGGIEHAILHLLY
ARFFTKALHDRNLIGFKEPFNRLLTQGMVQGLTYRNAKNGSYISPELVSDDSDPRDPESGDRLEVLFEKMSKSKYNGVDP
AVVIDRYGADTARMFILFKAPPEKDLEWDDADVEGQFRFLQRLIRLIDSFAWPKTDGENASISSANLIIDSADLSEEEIN
MRRATHKAIEAITEDLSGDIQLNTAISELMKLSNSLSGKLDKVRNEVAAEALSVLVRLMAPFAPHLAEEFWLKLHGHGSI
HQQSWPVIDPSALVLETIELVIQVKGKVRGKIQVPANADKKTLEELALNSDIAVKWLEGQSPRRIIIVPGKLVNLVP

Specific function: Unknown

COG id: COG0495

COG function: function code J; Leucyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI7661872, Length=879, Percent_Identity=41.8657565415245, Blast_Score=673, Evalue=0.0,
Organism=Homo sapiens, GI268370297, Length=885, Percent_Identity=21.9209039548023, Blast_Score=91, Evalue=7e-18,
Organism=Homo sapiens, GI268370293, Length=885, Percent_Identity=21.9209039548023, Blast_Score=91, Evalue=7e-18,
Organism=Escherichia coli, GI1786861, Length=876, Percent_Identity=44.7488584474886, Blast_Score=730, Evalue=0.0,
Organism=Escherichia coli, GI1790708, Length=377, Percent_Identity=23.8726790450928, Blast_Score=94, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI71997510, Length=845, Percent_Identity=31.4792899408284, Blast_Score=399, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI71997517, Length=845, Percent_Identity=31.4792899408284, Blast_Score=399, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI212645227, Length=380, Percent_Identity=31.0526315789474, Blast_Score=169, Evalue=5e-42,
Organism=Saccharomyces cerevisiae, GI6323414, Length=885, Percent_Identity=41.5819209039548, Blast_Score=657, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6321531, Length=379, Percent_Identity=21.6358839050132, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6325217, Length=145, Percent_Identity=26.2068965517241, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI21355409, Length=895, Percent_Identity=36.8715083798883, Blast_Score=518, Evalue=1e-147,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002300
- InterPro:   IPR002302
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155
- InterPro:   IPR009008 [H]

Pfam domain/function: PF08264 Anticodon_1; PF00133 tRNA-synt_1 [H]

EC number: =6.1.1.4 [H]

Molecular weight: Translated: 98559; Mature: 98427

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTESFPSTSASSQSSARYDPIELETRWQKEWLRQGLDRTPVAETNQKRFYALSMFPYPSG
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCC
KLHMGHVRNYVITDVIARVQRMRGDAVLHPMGWDAFGLPAENAAIARNVDPGDWTDQNIA
CEEHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCEEECCCCCCCCCHHHHH
QMRAQLDRLGLSIDWDRQQATCHQDYYRWTQWLFLELFAGGLAYQKEATVNWDPVDKTVL
HHHHHHHHCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEE
ANEQVDGEGRSWRSGALVEQRQLKQWFLRITDYADALIDDLDELTGWPERVRTMQANWIG
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCC
RSHGAEIKFRVAGVANSIITVFTTRPDTLHGASYVVLAPEHPLVESLTSPEQRLAVTAFC
CCCCCEEEEEEEECCCCEEEEEECCCCCCCCCEEEEECCCCHHHHHHCCHHHHHHHHHHH
DLISQLSVKDRTAEDQPKRGVPIGAQVINPVNGESLPVWIADYVLADYGSGAVMGVPAHD
HHHHHHCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHCCCCCEEECCCCC
ERDFIFARSHELPIRIVVQLPDTDEHHNDGQAWTGAGVLVNSGAFDGLSTEEAKVAITTH
CCCEEEEECCCCCEEEEEECCCCCCCCCCCCEEECCCEEEECCCCCCCCCCCCEEEEEEC
GASEGWAQSKVQYRLRDWLISRQRYWGCPIPIIHCASCGIVPVPQEDLPVTLPRDIDLSG
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCCCCEECCCCCCCCC
KGGSPIAQEQAWVEVKCPICGEKAHRETDTMDTFMCSSWYYLRFADPLNSQRPFDKDIVD
CCCCCCCCCCCEEEEECCCCCCHHCCCHHHHHHHHHCCEEEEEECCCCCCCCCCCHHHHH
EWLPVDQYVGGIEHAILHLLYARFFTKALHDRNLIGFKEPFNRLLTQGMVQGLTYRNAKN
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
GSYISPELVSDDSDPRDPESGDRLEVLFEKMSKSKYNGVDPAVVIDRYGADTARMFILFK
CCCCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCEEEEEEEE
APPEKDLEWDDADVEGQFRFLQRLIRLIDSFAWPKTDGENASISSANLIIDSADLSEEEI
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCHHHH
NMRRATHKAIEAITEDLSGDIQLNTAISELMKLSNSLSGKLDKVRNEVAAEALSVLVRLM
HHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
APFAPHLAEEFWLKLHGHGSIHQQSWPVIDPSALVLETIELVIQVKGKVRGKIQVPANAD
HHHCHHHHHHHEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCC
KKTLEELALNSDIAVKWLEGQSPRRIIIVPGKLVNLVP
HHHHHHHHCCCCEEEEEECCCCCCEEEEECCHHHCCCC
>Mature Secondary Structure 
TESFPSTSASSQSSARYDPIELETRWQKEWLRQGLDRTPVAETNQKRFYALSMFPYPSG
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCC
KLHMGHVRNYVITDVIARVQRMRGDAVLHPMGWDAFGLPAENAAIARNVDPGDWTDQNIA
CEEHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCEEECCCCCCCCCHHHHH
QMRAQLDRLGLSIDWDRQQATCHQDYYRWTQWLFLELFAGGLAYQKEATVNWDPVDKTVL
HHHHHHHHCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEE
ANEQVDGEGRSWRSGALVEQRQLKQWFLRITDYADALIDDLDELTGWPERVRTMQANWIG
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCC
RSHGAEIKFRVAGVANSIITVFTTRPDTLHGASYVVLAPEHPLVESLTSPEQRLAVTAFC
CCCCCEEEEEEEECCCCEEEEEECCCCCCCCCEEEEECCCCHHHHHHCCHHHHHHHHHHH
DLISQLSVKDRTAEDQPKRGVPIGAQVINPVNGESLPVWIADYVLADYGSGAVMGVPAHD
HHHHHHCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHCCCCCEEECCCCC
ERDFIFARSHELPIRIVVQLPDTDEHHNDGQAWTGAGVLVNSGAFDGLSTEEAKVAITTH
CCCEEEEECCCCCEEEEEECCCCCCCCCCCCEEECCCEEEECCCCCCCCCCCCEEEEEEC
GASEGWAQSKVQYRLRDWLISRQRYWGCPIPIIHCASCGIVPVPQEDLPVTLPRDIDLSG
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCCCCEECCCCCCCCC
KGGSPIAQEQAWVEVKCPICGEKAHRETDTMDTFMCSSWYYLRFADPLNSQRPFDKDIVD
CCCCCCCCCCCEEEEECCCCCCHHCCCHHHHHHHHHCCEEEEEECCCCCCCCCCCHHHHH
EWLPVDQYVGGIEHAILHLLYARFFTKALHDRNLIGFKEPFNRLLTQGMVQGLTYRNAKN
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
GSYISPELVSDDSDPRDPESGDRLEVLFEKMSKSKYNGVDPAVVIDRYGADTARMFILFK
CCCCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCEEEEEEEE
APPEKDLEWDDADVEGQFRFLQRLIRLIDSFAWPKTDGENASISSANLIIDSADLSEEEI
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCHHHH
NMRRATHKAIEAITEDLSGDIQLNTAISELMKLSNSLSGKLDKVRNEVAAEALSVLVRLM
HHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
APFAPHLAEEFWLKLHGHGSIHQQSWPVIDPSALVLETIELVIQVKGKVRGKIQVPANAD
HHHCHHHHHHHEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCC
KKTLEELALNSDIAVKWLEGQSPRRIIIVPGKLVNLVP
HHHHHHHHCCCCEEEEEECCCCCCEEEEECCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA