Click here to switch to the map view.

The map label for this gene is yjfH [C]

Identifier: 33862923

GI number: 33862923

Start: 704882

End: 706489

Strand: Reverse

Name: yjfH [C]

Synonym: PMT0650

Alternate gene names: 33862923

Gene position: 706489-704882 (Counterclockwise)

Preceding gene: 33862924

Following gene: 33862922

Centisome position: 29.3

GC content: 55.1

Gene sequence:

>1608_bases
ATGAGCTCCCGCTTTGATCGCCGTTCAGGCAACCCCCCTCGTGGTGGTCGTCCTAGAGGAGGTCGTCCTGGTGGGATCCG
ACGGGATGGCGATGGGGTTTCTGGTCGATCTGCTTACGGAAGCCGCTCTTCCGGCAGCCGCCCATCGGGTGGTCGTTCCT
CTGGACCGCGAGGGGTGTATCGCTCCAGGCCTGATGGAGAAGCTGGTGATAGCCCTTCAAAAGTATCCAATGGTGAAGGC
TCTGGGCAGGGCCGTTTTAACCGTGATCGTTCTCGCTCTGAGCAGGGGCGTTTTAGCCGGGATCGGCCTCGCTCTGAACA
AGGGCGTGCAAGTGAAACTGACAGAAAAGAGGCTCGTCGATTTCGTGATCAGAGGCCATCACGACGTCGCAGCGATGAAC
GGCTAGGGCGACGTCCCTCCTTGGGAGAACGTTCACGTCCTGCAGACAGGCAGTTGCCTTCAAAAAGATTCGATCCATCT
ACTTCGGATCGCGTTCCAGAGGCAGAAACATTCGGACCGGCTGCTGCCGATGATTTGCTTTGGGGACGTCATGCCAGCCA
AGCTGCTCTGGAAGCTGGCCGCCCGATTCATCGCATCTGGTGCACATCAGATTTACGAAGCGCACCAAGGTTTTTGCAAT
TGCTTCGTGAGGCCAAGTCCTCAGGAGTACTGGTGGAAGAAGTTACCTGGGCTCGTCTCGGTCAACTCACAGGTGGTGCT
GTGCATCAAGGAATTGTTCTACAGACTGCTGCCGCGGAGACCCTTGATTTGCAAAGTCTGATCGAGGGATGTGCGGGTCT
TGGGGAATCGCCCCTTTTACTTGCCCTCGATGGCCTAACGGATCCCCACAATCTTGGCGCCATTATTCGATCTGCAGAGG
CTCTAGGCGCCCATGGAGTTGTTCTCCCACAACGACGAAGTGCTGGTCTCACTGGTTCGGTGGCCAAGGTTGCAGCAGGC
GCTTTGGAGCATCTACCAGTAGCGCGTGTCGTGAATCTCAATCGCTCTTTAGAAGCACTGAAGCAGGCGGGATATCGAGT
TATTGGCCTGGCGGAGGAAGGGGACATCACCCTTCCAGATGTCGATCTAGACGGACCTTTAGTGATCGTAACGGGATCAG
AGAAGCAGGGGATCTCTCTCCTAACTCGTCGCCATTGCGATCAGCTTGTACGCGTTCCATTGCGGGGGGTTACTCCCAGT
CTGAATGCCTCTGTTGCTACTGCGATGTGTTTATACGAGGTTGCCCGTCGTGGCTGGATGAAGGGGGTTCATGGCCAAAC
TCCATCCCCCAGGATCGCGCGGCCTCAATGCCCTTTGCCGGTCATCTCTTGCGACTCCATTTCCGTGGAGTCAGAACCCA
TTGCCAAGACCAATCATTCGCCAATCCATCAGCCAGATGAGATTGAGCGGTTTGGGGAGGCTTTGCCATCCAGCTTGGCT
AACCAGCAACTTTGTTCCAAAGATGATGAACCACCGATGGATCGCCAAATAGGGGATTTGCAGGCAGAAAGAACTTCGAT
TGAGGATGAGGATTTGCTGACCAGTCAGGATTGCAATTTTGACGTTTGTGAGGATCAATCACCTGATGAAGAGAGTGTTC
AGCGTTGA

Upstream 100 bases:

>100_bases
TGGTTGGCTGGCTCTTTTTGCAGAATCCGGCGCGGCTTGCGCAGCTCTTGGATCAACTGGAGGAGACCGAACACGACTTG
CTATAGCTTCTTCTCTTCTT

Downstream 100 bases:

>100_bases
TCCAAAAATTCCTTCGCAGCCGACAGCGGAACACCAATTGGAACATGACGAGGCAAAATAATGCTCATCCGCAAACCTCA
ATCATGGGCTCATTGATTAG

Product: tRNA/rRNA methyltransferase (SpoU):RNA methyltransferase TrmH

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 535; Mature: 534

Protein sequence:

>535_residues
MSSRFDRRSGNPPRGGRPRGGRPGGIRRDGDGVSGRSAYGSRSSGSRPSGGRSSGPRGVYRSRPDGEAGDSPSKVSNGEG
SGQGRFNRDRSRSEQGRFSRDRPRSEQGRASETDRKEARRFRDQRPSRRRSDERLGRRPSLGERSRPADRQLPSKRFDPS
TSDRVPEAETFGPAAADDLLWGRHASQAALEAGRPIHRIWCTSDLRSAPRFLQLLREAKSSGVLVEEVTWARLGQLTGGA
VHQGIVLQTAAAETLDLQSLIEGCAGLGESPLLLALDGLTDPHNLGAIIRSAEALGAHGVVLPQRRSAGLTGSVAKVAAG
ALEHLPVARVVNLNRSLEALKQAGYRVIGLAEEGDITLPDVDLDGPLVIVTGSEKQGISLLTRRHCDQLVRVPLRGVTPS
LNASVATAMCLYEVARRGWMKGVHGQTPSPRIARPQCPLPVISCDSISVESEPIAKTNHSPIHQPDEIERFGEALPSSLA
NQQLCSKDDEPPMDRQIGDLQAERTSIEDEDLLTSQDCNFDVCEDQSPDEESVQR

Sequences:

>Translated_535_residues
MSSRFDRRSGNPPRGGRPRGGRPGGIRRDGDGVSGRSAYGSRSSGSRPSGGRSSGPRGVYRSRPDGEAGDSPSKVSNGEG
SGQGRFNRDRSRSEQGRFSRDRPRSEQGRASETDRKEARRFRDQRPSRRRSDERLGRRPSLGERSRPADRQLPSKRFDPS
TSDRVPEAETFGPAAADDLLWGRHASQAALEAGRPIHRIWCTSDLRSAPRFLQLLREAKSSGVLVEEVTWARLGQLTGGA
VHQGIVLQTAAAETLDLQSLIEGCAGLGESPLLLALDGLTDPHNLGAIIRSAEALGAHGVVLPQRRSAGLTGSVAKVAAG
ALEHLPVARVVNLNRSLEALKQAGYRVIGLAEEGDITLPDVDLDGPLVIVTGSEKQGISLLTRRHCDQLVRVPLRGVTPS
LNASVATAMCLYEVARRGWMKGVHGQTPSPRIARPQCPLPVISCDSISVESEPIAKTNHSPIHQPDEIERFGEALPSSLA
NQQLCSKDDEPPMDRQIGDLQAERTSIEDEDLLTSQDCNFDVCEDQSPDEESVQR
>Mature_534_residues
SSRFDRRSGNPPRGGRPRGGRPGGIRRDGDGVSGRSAYGSRSSGSRPSGGRSSGPRGVYRSRPDGEAGDSPSKVSNGEGS
GQGRFNRDRSRSEQGRFSRDRPRSEQGRASETDRKEARRFRDQRPSRRRSDERLGRRPSLGERSRPADRQLPSKRFDPST
SDRVPEAETFGPAAADDLLWGRHASQAALEAGRPIHRIWCTSDLRSAPRFLQLLREAKSSGVLVEEVTWARLGQLTGGAV
HQGIVLQTAAAETLDLQSLIEGCAGLGESPLLLALDGLTDPHNLGAIIRSAEALGAHGVVLPQRRSAGLTGSVAKVAAGA
LEHLPVARVVNLNRSLEALKQAGYRVIGLAEEGDITLPDVDLDGPLVIVTGSEKQGISLLTRRHCDQLVRVPLRGVTPSL
NASVATAMCLYEVARRGWMKGVHGQTPSPRIARPQCPLPVISCDSISVESEPIAKTNHSPIHQPDEIERFGEALPSSLAN
QQLCSKDDEPPMDRQIGDLQAERTSIEDEDLLTSQDCNFDVCEDQSPDEESVQR

Specific function: Unknown

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=277, Percent_Identity=28.158844765343, Blast_Score=80, Evalue=7e-15,
Organism=Escherichia coli, GI1790623, Length=241, Percent_Identity=40.2489626556017, Blast_Score=171, Evalue=1e-43,
Organism=Escherichia coli, GI1788935, Length=314, Percent_Identity=27.7070063694268, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1790083, Length=149, Percent_Identity=31.5436241610738, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17555908, Length=320, Percent_Identity=27.8125, Blast_Score=81, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324775, Length=256, Percent_Identity=31.640625, Blast_Score=96, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 57857; Mature: 57726

Theoretical pI: Translated: 8.34; Mature: 8.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSRFDRRSGNPPRGGRPRGGRPGGIRRDGDGVSGRSAYGSRSSGSRPSGGRSSGPRGVY
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
RSRPDGEAGDSPSKVSNGEGSGQGRFNRDRSRSEQGRFSRDRPRSEQGRASETDRKEARR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHH
FRDQRPSRRRSDERLGRRPSLGERSRPADRQLPSKRFDPSTSDRVPEAETFGPAAADDLL
HHHCCCHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCHHHCCCCHHHHHH
WGRHASQAALEAGRPIHRIWCTSDLRSAPRFLQLLREAKSSGVLVEEVTWARLGQLTGGA
HCCCHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHCCEEHHHHHHHHHHHHCCCH
VHQGIVLQTAAAETLDLQSLIEGCAGLGESPLLLALDGLTDPHNLGAIIRSAEALGAHGV
HHCCEEEEHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCE
VLPQRRSAGLTGSVAKVAAGALEHLPVARVVNLNRSLEALKQAGYRVIGLAEEGDITLPD
EECCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCEECCC
VDLDGPLVIVTGSEKQGISLLTRRHCDQLVRVPLRGVTPSLNASVATAMCLYEVARRGWM
CCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
KGVHGQTPSPRIARPQCPLPVISCDSISVESEPIAKTNHSPIHQPDEIERFGEALPSSLA
CCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
NQQLCSKDDEPPMDRQIGDLQAERTSIEDEDLLTSQDCNFDVCEDQSPDEESVQR
HHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCHHHHCC
>Mature Secondary Structure 
SSRFDRRSGNPPRGGRPRGGRPGGIRRDGDGVSGRSAYGSRSSGSRPSGGRSSGPRGVY
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
RSRPDGEAGDSPSKVSNGEGSGQGRFNRDRSRSEQGRFSRDRPRSEQGRASETDRKEARR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHH
FRDQRPSRRRSDERLGRRPSLGERSRPADRQLPSKRFDPSTSDRVPEAETFGPAAADDLL
HHHCCCHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCHHHCCCCHHHHHH
WGRHASQAALEAGRPIHRIWCTSDLRSAPRFLQLLREAKSSGVLVEEVTWARLGQLTGGA
HCCCHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHCCEEHHHHHHHHHHHHCCCH
VHQGIVLQTAAAETLDLQSLIEGCAGLGESPLLLALDGLTDPHNLGAIIRSAEALGAHGV
HHCCEEEEHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCE
VLPQRRSAGLTGSVAKVAAGALEHLPVARVVNLNRSLEALKQAGYRVIGLAEEGDITLPD
EECCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCEECCC
VDLDGPLVIVTGSEKQGISLLTRRHCDQLVRVPLRGVTPSLNASVATAMCLYEVARRGWM
CCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
KGVHGQTPSPRIARPQCPLPVISCDSISVESEPIAKTNHSPIHQPDEIERFGEALPSSLA
CCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
NQQLCSKDDEPPMDRQIGDLQAERTSIEDEDLLTSQDCNFDVCEDQSPDEESVQR
HHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]