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The map label for this gene is gatA [H]

Identifier: 33862921

GI number: 33862921

Start: 703044

End: 704504

Strand: Reverse

Name: gatA [H]

Synonym: PMT0648

Alternate gene names: 33862921

Gene position: 704504-703044 (Counterclockwise)

Preceding gene: 33862922

Following gene: 33862920

Centisome position: 29.22

GC content: 55.44

Gene sequence:

>1461_bases
ATGGCGATCGCTGAATGGCGTCAGCAACTAAAGCTCGGCGAGGTTTCCGCCAGGGAACTCACAGATCACCAACTTGCTCG
TATCGCAGTGGTGGATCCCACTCTGCACGCTTTTCTGGATATCACAGCAGATCGAGCTCGTGCCGATGCCGATCGCATCG
ATGAGGCCTTGGCTGCGGGTGAGTCTCTGCCACCTCTGGCTGGTGTGCCTTTGGCAATTAAGGACAACCTCTGCACGAAA
GGAATACGCACCACCTGTTCCAGTCGAATGCTGGAAACCTTTGTGCCGCCCTATGAATCCACCGTCACTGAGCGCCTCTG
GCAGGCCGGTGCTGTACTTCTCGGGAAAACAAATCTCGATGAGTTCGCCATGGGTAGCTCGACCGAGACATCTGCGTTTG
GGGCTACATCCAATCCCTGGGATATCAGCCGTGTGCCGGGTGGCAGTTCTGGTGGCAGTGCAGCAGCTGTTGCGGCAGGT
GAATGCATGGCTGCCTTGGGTTCGGATACTGGGGGCTCAATCCGTCAGCCCGCTTCATTTTGTGGAGTCGTAGGCCTTAA
ACCCACCTATGGCCGTGTCAGTCGTTGGGGTTTGGTGGCTTTTGCCAGTTCTCTTGACCAGGTTGGACCATTCACGACCA
ACGTGGCCGATGCTGCTGAGCTTTTACAAGTGATCGCAGGATCTGATCCTCGTGACTCCACTTGTCTCAATGTTGCTGTT
CCCGATTATTGCTCTGCCCTTTCGCAACCAATGTCTGGGGTACGCATCGGCTTGATTCGTGAGTGCTTTGACCAAAACGG
CTTGGATGCGCAAGTGAAGAGAACAGTTTTGACAGCTGCAGAGAAGCTGCAATCCTTGGGAGCGGAACTGGTTGAGGTGA
GTTGCCCACGCTTTAGCGATGGCATCGCCACCTATTACGTGATTGCCCCATCGGAGGCTTCGGCCAACTTGGCGCGTTAC
GACGGGGTGAAGTACGGCTACCGAGCTGAAGGAGCTGATGCTCTAGCCGCGATGACAGCCCGAAGTCGCGCAGAGGGTTT
TGGTAGCGAGGTGCAGCGACGCATCCTGATCGGCACCTATGCCCTCTCGGCTGGTTACATGGACGCTTACTACAAGAAAG
CCCAGCAGGTCCGCACATTGATCCGTCAAGATTTTGATGCAGCCTTCCAAACGGTCGATGTGCTGCTGACACCCACATCA
CCTACCACTGCATTCAAAGCCGGTGCCCACGCTGATGATCCACTTGCCATGTACCTGGCTGACCTGCTGACGATCCCAGC
CAACCTGGCTGGCTTACCTGCCATCAGTCTTCCCTGTGGATTCGATGACGATGGCCTTCCGATCGGGGTGCAGTTGATTG
CAAATGTGCTGGAGGAGTCACGCCTCCTACAAGTGGCTTTTCACTATGAGCAAGCAGCAAACGTTATGGCAAATCACCCG
CAGGGAAACTTCATTCCCTAA

Upstream 100 bases:

>100_bases
CCTCGTGCGTTGTCGTCGCGTTGAGCCGCTGACGATCGCAGTCGAAGGCTGGTCAGACGACTGATAGCGTCGCTGCTGCA
CAGCACAGCACCGCAAGCCG

Downstream 100 bases:

>100_bases
ACGATCAATTGCACTAGATGCAGCGTTTGGCTACATGAACAACCCTGCATCTTGCGCCTCCTCCTAGGCTGGGGGCATGG
CATTCGTTCCTCTTCACAAC

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MAIAEWRQQLKLGEVSARELTDHQLARIAVVDPTLHAFLDITADRARADADRIDEALAAGESLPPLAGVPLAIKDNLCTK
GIRTTCSSRMLETFVPPYESTVTERLWQAGAVLLGKTNLDEFAMGSSTETSAFGATSNPWDISRVPGGSSGGSAAAVAAG
ECMAALGSDTGGSIRQPASFCGVVGLKPTYGRVSRWGLVAFASSLDQVGPFTTNVADAAELLQVIAGSDPRDSTCLNVAV
PDYCSALSQPMSGVRIGLIRECFDQNGLDAQVKRTVLTAAEKLQSLGAELVEVSCPRFSDGIATYYVIAPSEASANLARY
DGVKYGYRAEGADALAAMTARSRAEGFGSEVQRRILIGTYALSAGYMDAYYKKAQQVRTLIRQDFDAAFQTVDVLLTPTS
PTTAFKAGAHADDPLAMYLADLLTIPANLAGLPAISLPCGFDDDGLPIGVQLIANVLEESRLLQVAFHYEQAANVMANHP
QGNFIP

Sequences:

>Translated_486_residues
MAIAEWRQQLKLGEVSARELTDHQLARIAVVDPTLHAFLDITADRARADADRIDEALAAGESLPPLAGVPLAIKDNLCTK
GIRTTCSSRMLETFVPPYESTVTERLWQAGAVLLGKTNLDEFAMGSSTETSAFGATSNPWDISRVPGGSSGGSAAAVAAG
ECMAALGSDTGGSIRQPASFCGVVGLKPTYGRVSRWGLVAFASSLDQVGPFTTNVADAAELLQVIAGSDPRDSTCLNVAV
PDYCSALSQPMSGVRIGLIRECFDQNGLDAQVKRTVLTAAEKLQSLGAELVEVSCPRFSDGIATYYVIAPSEASANLARY
DGVKYGYRAEGADALAAMTARSRAEGFGSEVQRRILIGTYALSAGYMDAYYKKAQQVRTLIRQDFDAAFQTVDVLLTPTS
PTTAFKAGAHADDPLAMYLADLLTIPANLAGLPAISLPCGFDDDGLPIGVQLIANVLEESRLLQVAFHYEQAANVMANHP
QGNFIP
>Mature_485_residues
AIAEWRQQLKLGEVSARELTDHQLARIAVVDPTLHAFLDITADRARADADRIDEALAAGESLPPLAGVPLAIKDNLCTKG
IRTTCSSRMLETFVPPYESTVTERLWQAGAVLLGKTNLDEFAMGSSTETSAFGATSNPWDISRVPGGSSGGSAAAVAAGE
CMAALGSDTGGSIRQPASFCGVVGLKPTYGRVSRWGLVAFASSLDQVGPFTTNVADAAELLQVIAGSDPRDSTCLNVAVP
DYCSALSQPMSGVRIGLIRECFDQNGLDAQVKRTVLTAAEKLQSLGAELVEVSCPRFSDGIATYYVIAPSEASANLARYD
GVKYGYRAEGADALAAMTARSRAEGFGSEVQRRILIGTYALSAGYMDAYYKKAQQVRTLIRQDFDAAFQTVDVLLTPTSP
TTAFKAGAHADDPLAMYLADLLTIPANLAGLPAISLPCGFDDDGLPIGVQLIANVLEESRLLQVAFHYEQAANVMANHPQ
GNFIP

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=487, Percent_Identity=39.4250513347023, Blast_Score=318, Evalue=5e-87,
Organism=Homo sapiens, GI195972892, Length=492, Percent_Identity=25.609756097561, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI166795287, Length=232, Percent_Identity=33.1896551724138, Blast_Score=72, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17543272, Length=440, Percent_Identity=39.3181818181818, Blast_Score=274, Evalue=9e-74,
Organism=Caenorhabditis elegans, GI17537465, Length=492, Percent_Identity=24.5934959349593, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17556264, Length=235, Percent_Identity=32.3404255319149, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI71990152, Length=265, Percent_Identity=31.6981132075472, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17538252, Length=318, Percent_Identity=25.1572327044025, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17556276, Length=268, Percent_Identity=30.2238805970149, Blast_Score=79, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17556278, Length=268, Percent_Identity=30.2238805970149, Blast_Score=79, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17538254, Length=169, Percent_Identity=30.1775147928994, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6323950, Length=419, Percent_Identity=35.5608591885441, Blast_Score=249, Evalue=5e-67,
Organism=Saccharomyces cerevisiae, GI6319685, Length=455, Percent_Identity=32.0879120879121, Blast_Score=140, Evalue=6e-34,
Organism=Saccharomyces cerevisiae, GI6320448, Length=190, Percent_Identity=35.7894736842105, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24648113, Length=473, Percent_Identity=44.1860465116279, Blast_Score=333, Evalue=2e-91,
Organism=Drosophila melanogaster, GI24644968, Length=492, Percent_Identity=27.4390243902439, Blast_Score=144, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24652985, Length=493, Percent_Identity=25.7606490872211, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI19922090, Length=493, Percent_Identity=25.7606490872211, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24652981, Length=493, Percent_Identity=25.7606490872211, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24652983, Length=493, Percent_Identity=25.7606490872211, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI45550774, Length=490, Percent_Identity=25.3061224489796, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24648435, Length=490, Percent_Identity=25.3061224489796, Blast_Score=126, Evalue=4e-29,
Organism=Drosophila melanogaster, GI24648437, Length=490, Percent_Identity=25.3061224489796, Blast_Score=126, Evalue=4e-29,
Organism=Drosophila melanogaster, GI161078093, Length=504, Percent_Identity=26.5873015873016, Blast_Score=122, Evalue=7e-28,
Organism=Drosophila melanogaster, GI24648441, Length=432, Percent_Identity=26.1574074074074, Blast_Score=111, Evalue=9e-25,
Organism=Drosophila melanogaster, GI24648439, Length=432, Percent_Identity=26.1574074074074, Blast_Score=111, Evalue=9e-25,
Organism=Drosophila melanogaster, GI21356731, Length=487, Percent_Identity=24.8459958932238, Blast_Score=102, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 51395; Mature: 51263

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIAEWRQQLKLGEVSARELTDHQLARIAVVDPTLHAFLDITADRARADADRIDEALAAG
CCHHHHHHHHHHCCCHHHHHHHHHHHEEEEECHHHHHHHHHHHHHHHCCHHHHHHHHHCC
ESLPPLAGVPLAIKDNLCTKGIRTTCSSRMLETFVPPYESTVTERLWQAGAVLLGKTNLD
CCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEECCCCH
EFAMGSSTETSAFGATSNPWDISRVPGGSSGGSAAAVAAGECMAALGSDTGGSIRQPASF
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHH
CGVVGLKPTYGRVSRWGLVAFASSLDQVGPFTTNVADAAELLQVIAGSDPRDSTCLNVAV
HCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEC
PDYCSALSQPMSGVRIGLIRECFDQNGLDAQVKRTVLTAAEKLQSLGAELVEVSCPRFSD
HHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GIATYYVIAPSEASANLARYDGVKYGYRAEGADALAAMTARSRAEGFGSEVQRRILIGTY
CCEEEEEEECCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
ALSAGYMDAYYKKAQQVRTLIRQDFDAAFQTVDVLLTPTSPTTAFKAGAHADDPLAMYLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCHHHCCCCCCCCHHHHHHH
DLLTIPANLAGLPAISLPCGFDDDGLPIGVQLIANVLEESRLLQVAFHYEQAANVMANHP
HHHHHCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
QGNFIP
CCCCCC
>Mature Secondary Structure 
AIAEWRQQLKLGEVSARELTDHQLARIAVVDPTLHAFLDITADRARADADRIDEALAAG
CHHHHHHHHHHCCCHHHHHHHHHHHEEEEECHHHHHHHHHHHHHHHCCHHHHHHHHHCC
ESLPPLAGVPLAIKDNLCTKGIRTTCSSRMLETFVPPYESTVTERLWQAGAVLLGKTNLD
CCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEECCCCH
EFAMGSSTETSAFGATSNPWDISRVPGGSSGGSAAAVAAGECMAALGSDTGGSIRQPASF
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHH
CGVVGLKPTYGRVSRWGLVAFASSLDQVGPFTTNVADAAELLQVIAGSDPRDSTCLNVAV
HCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEC
PDYCSALSQPMSGVRIGLIRECFDQNGLDAQVKRTVLTAAEKLQSLGAELVEVSCPRFSD
HHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GIATYYVIAPSEASANLARYDGVKYGYRAEGADALAAMTARSRAEGFGSEVQRRILIGTY
CCEEEEEEECCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
ALSAGYMDAYYKKAQQVRTLIRQDFDAAFQTVDVLLTPTSPTTAFKAGAHADDPLAMYLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCHHHCCCCCCCCHHHHHHH
DLLTIPANLAGLPAISLPCGFDDDGLPIGVQLIANVLEESRLLQVAFHYEQAANVMANHP
HHHHHCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
QGNFIP
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA