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The map label for this gene is spt [H]

Identifier: 33862873

GI number: 33862873

Start: 655810

End: 656994

Strand: Reverse

Name: spt [H]

Synonym: PMT0600

Alternate gene names: 33862873

Gene position: 656994-655810 (Counterclockwise)

Preceding gene: 33862875

Following gene: 33862871

Centisome position: 27.25

GC content: 53.42

Gene sequence:

>1185_bases
TTGGCGACGACGCAAACCCTTCCCCAGGTTGACCAATCTCATAAGACGGTTCTCGAACCGATTGCCACGCCTAGCCGCTT
GCTATTTGGGCCTGGACCCTCAAATGCCGATCCCAAGGTGCTTCAGGCACTTGCGCGCTCTCCGATTGGCCATCTTGATC
CCCTTTATCTGGAATTGATGGGTGAGGTTCAGGAGTTGCTCCGCTACACCTGGCAGACCGACAACCGTTTCACACTGCCG
ATGAGCGGTACTGGTAGTGCCGCCATGGAGGCGACCTTGGCCAATACCGTTGAACCTGGTGACACGGTATTGGTTGCTGT
AAACGGTTATTTCGGCAATCGCCTTGCCGATATGGCTGCTCGCTATCGAGCCAATGTTCAGTTGATTGAAAAACCCTGGG
GAGAAGCCTTTTCCCTTGCAGAGCTAGAAGCAGCTTTGAGCGAGCATCGTCCAGCAATTCTGGCTCTAGTGCATGCAGAA
ACATCCACTGGCGTTTGTCAGCCAATGGATGGGGTCGGCGATCTTTGCCATAAGCACGATTGCCTCTTACTGCTTGATAC
GGTCACCTCCTTAGGCGCTGTACCGGTTTTTCTGGATCAATGGAAGGTTGATCTGTCTTACAGCTGCAGCCAGAAGGGGC
TCAGCTGCCCTCCTGGTCTTGGCCCTTTCACCATGGGACCCCGCGCCGAAGAGAAGCTTGTGGCCCGTAGTAGCAAGGTG
CCCAATTGGTATCTAGACGTGTCGCTGCTGAATCAGTATTGGGGAAGCGACAGGGTTTATCACCACACTGCGCCGGTGAA
TATGAACTTTGGGATGCGTGAAGCGCTGCGATTACTTGCGGATGAGGGCCTTGAAAACGCTTGGGATCGTCATCGCTGCA
ATGCCGAAGTGCTCTGGGCAGGGCTCGAGAGTCTTGGTTTGGAGTTGCATGTACCTGAAGCCTTGCGTTTGCCCACACTC
ACAACTGTTTGCATTCCTAATGGAGTTGATGGCAATGCCTTCCGTCTGCATTTGCTGAATGAGCACGGCATTGAAATCGG
CGGCGGCTTAGGAAGCTTGGCTGGCAAGATCTGGAGGATTGGCTTGATGGGATACAACTCCACCCCTAAAAACGTTGAGC
TGTTGCTCAATCTGTTCGAGTCAGAGTTACCTCGTTTTCGTGAAAACGTTGCCGTTGCTGCTTGA

Upstream 100 bases:

>100_bases
TATTGATTGACCTGCTTGGTTGCCGTTGCAATCCCTCAAAAAAGTCCCGGGGATGAAGGGTGACTGCTTAGGCTCTAGAT
AATTGAACTAACCAAGGGCC

Downstream 100 bases:

>100_bases
ACCAGGCTTCTAGCTGCTGCCTAGCTTCAACTTCCATCACACCGCCAAGAACACGCATTTTATGATGTGCACTGGGGTGT
TCCCCCAGATGGATTGTGCT

Product: serine:pyruvate/alanine:glyoxylate aminotransferase

Products: pyruvate; glycine

Alternate protein names: NA

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MATTQTLPQVDQSHKTVLEPIATPSRLLFGPGPSNADPKVLQALARSPIGHLDPLYLELMGEVQELLRYTWQTDNRFTLP
MSGTGSAAMEATLANTVEPGDTVLVAVNGYFGNRLADMAARYRANVQLIEKPWGEAFSLAELEAALSEHRPAILALVHAE
TSTGVCQPMDGVGDLCHKHDCLLLLDTVTSLGAVPVFLDQWKVDLSYSCSQKGLSCPPGLGPFTMGPRAEEKLVARSSKV
PNWYLDVSLLNQYWGSDRVYHHTAPVNMNFGMREALRLLADEGLENAWDRHRCNAEVLWAGLESLGLELHVPEALRLPTL
TTVCIPNGVDGNAFRLHLLNEHGIEIGGGLGSLAGKIWRIGLMGYNSTPKNVELLLNLFESELPRFRENVAVAA

Sequences:

>Translated_394_residues
MATTQTLPQVDQSHKTVLEPIATPSRLLFGPGPSNADPKVLQALARSPIGHLDPLYLELMGEVQELLRYTWQTDNRFTLP
MSGTGSAAMEATLANTVEPGDTVLVAVNGYFGNRLADMAARYRANVQLIEKPWGEAFSLAELEAALSEHRPAILALVHAE
TSTGVCQPMDGVGDLCHKHDCLLLLDTVTSLGAVPVFLDQWKVDLSYSCSQKGLSCPPGLGPFTMGPRAEEKLVARSSKV
PNWYLDVSLLNQYWGSDRVYHHTAPVNMNFGMREALRLLADEGLENAWDRHRCNAEVLWAGLESLGLELHVPEALRLPTL
TTVCIPNGVDGNAFRLHLLNEHGIEIGGGLGSLAGKIWRIGLMGYNSTPKNVELLLNLFESELPRFRENVAVAA
>Mature_393_residues
ATTQTLPQVDQSHKTVLEPIATPSRLLFGPGPSNADPKVLQALARSPIGHLDPLYLELMGEVQELLRYTWQTDNRFTLPM
SGTGSAAMEATLANTVEPGDTVLVAVNGYFGNRLADMAARYRANVQLIEKPWGEAFSLAELEAALSEHRPAILALVHAET
STGVCQPMDGVGDLCHKHDCLLLLDTVTSLGAVPVFLDQWKVDLSYSCSQKGLSCPPGLGPFTMGPRAEEKLVARSSKVP
NWYLDVSLLNQYWGSDRVYHHTAPVNMNFGMREALRLLADEGLENAWDRHRCNAEVLWAGLESLGLELHVPEALRLPTLT
TVCIPNGVDGNAFRLHLLNEHGIEIGGGLGSLAGKIWRIGLMGYNSTPKNVELLLNLFESELPRFRENVAVAA

Specific function: Could encode ureidoglycolase or L-alanine:glyoxylate aminotransferase, or perhaps both activities [H]

COG id: COG0075

COG function: function code E; Serine-pyruvate aminotransferase/archaeal aspartate aminotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI4557289, Length=373, Percent_Identity=43.1635388739946, Blast_Score=325, Evalue=4e-89,
Organism=Caenorhabditis elegans, GI17536281, Length=373, Percent_Identity=41.2868632707775, Blast_Score=313, Evalue=1e-85,
Organism=Saccharomyces cerevisiae, GI6321079, Length=373, Percent_Identity=26.2734584450402, Blast_Score=111, Evalue=2e-25,
Organism=Drosophila melanogaster, GI17530823, Length=365, Percent_Identity=40.5479452054795, Blast_Score=299, Evalue=3e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: 2.6.1.44

Molecular weight: Translated: 43034; Mature: 42903

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATTQTLPQVDQSHKTVLEPIATPSRLLFGPGPSNADPKVLQALARSPIGHLDPLYLELM
CCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHCCCCCCCHHHHHHH
GEVQELLRYTWQTDNRFTLPMSGTGSAAMEATLANTVEPGDTVLVAVNGYFGNRLADMAA
HHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHH
RYRANVQLIEKPWGEAFSLAELEAALSEHRPAILALVHAETSTGVCQPMDGVGDLCHKHD
HHHCCEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCHHHHHHHHCCC
CLLLLDTVTSLGAVPVFLDQWKVDLSYSCSQKGLSCPPGLGPFTMGPRAEEKLVARSSKV
CEEEEHHHHHHCCCEEEEECEEECEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCC
PNWYLDVSLLNQYWGSDRVYHHTAPVNMNFGMREALRLLADEGLENAWDRHRCNAEVLWA
CCEEEEHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHCCHHHHHHHHCCCHHHEEH
GLESLGLELHVPEALRLPTLTTVCIPNGVDGNAFRLHLLNEHGIEIGGGLGSLAGKIWRI
HHHHCCCEEECCCHHCCCCEEEEEECCCCCCCEEEEEEECCCCCEECCCHHHHHHHHEEE
GLMGYNSTPKNVELLLNLFESELPRFRENVAVAA
EEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ATTQTLPQVDQSHKTVLEPIATPSRLLFGPGPSNADPKVLQALARSPIGHLDPLYLELM
CCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHCCCCCCCHHHHHHH
GEVQELLRYTWQTDNRFTLPMSGTGSAAMEATLANTVEPGDTVLVAVNGYFGNRLADMAA
HHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHH
RYRANVQLIEKPWGEAFSLAELEAALSEHRPAILALVHAETSTGVCQPMDGVGDLCHKHD
HHHCCEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCHHHHHHHHCCC
CLLLLDTVTSLGAVPVFLDQWKVDLSYSCSQKGLSCPPGLGPFTMGPRAEEKLVARSSKV
CEEEEHHHHHHCCCEEEEECEEECEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCC
PNWYLDVSLLNQYWGSDRVYHHTAPVNMNFGMREALRLLADEGLENAWDRHRCNAEVLWA
CCEEEEHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHCCHHHHHHHHCCCHHHEEH
GLESLGLELHVPEALRLPTLTTVCIPNGVDGNAFRLHLLNEHGIEIGGGLGSLAGKIWRI
HHHHCCCEEECCCHHCCCCEEEEEECCCCCCCEEEEEEECCCCCEECCCHHHHHHHHEEE
GLMGYNSTPKNVELLLNLFESELPRFRENVAVAA
EEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-alanine; glyoxylate

Specific reaction: L-alanine + glyoxylate = pyruvate + glycine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11344136 [H]