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The map label for this gene is trpA

Identifier: 33862738

GI number: 33862738

Start: 517240

End: 518067

Strand: Reverse

Name: trpA

Synonym: PMT0465

Alternate gene names: 33862738

Gene position: 518067-517240 (Counterclockwise)

Preceding gene: 33862739

Following gene: 33862736

Centisome position: 21.49

GC content: 53.5

Gene sequence:

>828_bases
ATGGCAGATTTCTTGGCACCTAAATCCAATACACACGCCACAATCAGCAGTCGCTTCCAACGAGTACAAGCTGAGGGACG
CCTTGCACTCATGCCATTCCTCATGGCTGGTGATCCTGATCTGGAAACCACTGCTGAGGTATTGCTCAGCCTGGAGCAAT
CTGGCGCGGACATGATCGAGCTGGGCATTCCCTATAGCGATCCTCTCGCCGATGGTCCGGTAATTCAGGCAGCTGCTTCT
CGAGCACTGGCTTCTGGTACGACACCAGCTCGGGTCTTGCAGATGTTGATTGACTTGCGAGGCAAACTTTCCATTCCTGT
GATCCTGTTCACCTACACCAATCCATTGTTCAACCGGGGTATGGAGCGTTTCTGTGATGAAGCTGCTGAGGCTGGAGTTG
CCGGTCTTGTGGTCCCAGATCTTCCACTCGAGGAAGCTGAGCGCCTTTCCCCCTTGGCTTCTGCTCGTGGACTTGATCTG
GTTCTTCTGGTGGCTCCTACTACACCTGCGGAGCGTATGGCAAGGATTGCAGAAAGCAGCAGGGGTTTTACCTATTTAGT
AAGCGTGACGGGAGTTACTGGCGAACGTTCTGTAATGGAGGATCGTGTTCAATCCTTGGTGCAGCAACTCAAGTTGTCCG
GCTCCAATCCTGTAGCAGTCGGATTTGGAATTTCTGGGCCTCAGCAAGTACGGCAGGTGCGAAGTTGGGGTGCTGATGGA
GCCATTGTTGGCAGTGCTCTAGTGAAACGAATGGCTGCTGCGGCTCCTGGATTAGTTGCTCAAGAAGCGGGTCTTTTCTG
CAAGGAGCTGCGCAACGCTGCTGGATGA

Upstream 100 bases:

>100_bases
CTGAAGGATATTGAGGATGAGAATCCTAAGCAACAAGCCCCCTCTGACCCATAGGTTCTAGGGCGTTTGCCCCCCTCACG
CCTTCTTCTAATTGATTGCT

Downstream 100 bases:

>100_bases
GTTTTAACTTGCTCATATCTATCATCCTGCCTTAAGACACAACAAAAAAAGCCCTGGAAGATTTCCAGGGCTTGCTTGAT
TCAAATGAGGTTGGAACGAT

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MADFLAPKSNTHATISSRFQRVQAEGRLALMPFLMAGDPDLETTAEVLLSLEQSGADMIELGIPYSDPLADGPVIQAAAS
RALASGTTPARVLQMLIDLRGKLSIPVILFTYTNPLFNRGMERFCDEAAEAGVAGLVVPDLPLEEAERLSPLASARGLDL
VLLVAPTTPAERMARIAESSRGFTYLVSVTGVTGERSVMEDRVQSLVQQLKLSGSNPVAVGFGISGPQQVRQVRSWGADG
AIVGSALVKRMAAAAPGLVAQEAGLFCKELRNAAG

Sequences:

>Translated_275_residues
MADFLAPKSNTHATISSRFQRVQAEGRLALMPFLMAGDPDLETTAEVLLSLEQSGADMIELGIPYSDPLADGPVIQAAAS
RALASGTTPARVLQMLIDLRGKLSIPVILFTYTNPLFNRGMERFCDEAAEAGVAGLVVPDLPLEEAERLSPLASARGLDL
VLLVAPTTPAERMARIAESSRGFTYLVSVTGVTGERSVMEDRVQSLVQQLKLSGSNPVAVGFGISGPQQVRQVRSWGADG
AIVGSALVKRMAAAAPGLVAQEAGLFCKELRNAAG
>Mature_274_residues
ADFLAPKSNTHATISSRFQRVQAEGRLALMPFLMAGDPDLETTAEVLLSLEQSGADMIELGIPYSDPLADGPVIQAAASR
ALASGTTPARVLQMLIDLRGKLSIPVILFTYTNPLFNRGMERFCDEAAEAGVAGLVVPDLPLEEAERLSPLASARGLDLV
LLVAPTTPAERMARIAESSRGFTYLVSVTGVTGERSVMEDRVQSLVQQLKLSGSNPVAVGFGISGPQQVRQVRSWGADGA
IVGSALVKRMAAAAPGLVAQEAGLFCKELRNAAG

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=234, Percent_Identity=39.7435897435897, Blast_Score=159, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6321412, Length=258, Percent_Identity=37.2093023255814, Blast_Score=172, Evalue=4e-44,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_PROMM (Q7TV26)

Other databases:

- EMBL:   BX548175
- RefSeq:   NP_894298.1
- ProteinModelPortal:   Q7TV26
- STRING:   Q7TV26
- GeneID:   1728268
- GenomeReviews:   BX548175_GR
- KEGG:   pmt:PMT0465
- NMPDR:   fig|74547.1.peg.465
- eggNOG:   COG0159
- HOGENOM:   HBG391690
- OMA:   NPIYIYG
- ProtClustDB:   PRK13111
- BioCyc:   PMAR74547:PMT0465-MONOMER
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 28959; Mature: 28828

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 60-60 ACT_SITE 71-71

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADFLAPKSNTHATISSRFQRVQAEGRLALMPFLMAGDPDLETTAEVLLSLEQSGADMIE
CCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEHHHHCCCCCHHHHHHHHHHHHHCCCCEEE
LGIPYSDPLADGPVIQAAASRALASGTTPARVLQMLIDLRGKLSIPVILFTYTNPLFNRG
ECCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHH
MERFCDEAAEAGVAGLVVPDLPLEEAERLSPLASARGLDLVLLVAPTTPAERMARIAESS
HHHHHHHHHHCCCCEEEECCCCHHHHHHHCHHHHCCCCEEEEEEECCCCHHHHHHHHHCC
RGFTYLVSVTGVTGERSVMEDRVQSLVQQLKLSGSNPVAVGFGISGPQQVRQVRSWGADG
CCEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHCCCCC
AIVGSALVKRMAAAAPGLVAQEAGLFCKELRNAAG
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ADFLAPKSNTHATISSRFQRVQAEGRLALMPFLMAGDPDLETTAEVLLSLEQSGADMIE
CCCCCCCCCCCHHHHHHHHHHHHCCCEEEEHHHHCCCCCHHHHHHHHHHHHHCCCCEEE
LGIPYSDPLADGPVIQAAASRALASGTTPARVLQMLIDLRGKLSIPVILFTYTNPLFNRG
ECCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHH
MERFCDEAAEAGVAGLVVPDLPLEEAERLSPLASARGLDLVLLVAPTTPAERMARIAESS
HHHHHHHHHHCCCCEEEECCCCHHHHHHHCHHHHCCCCEEEEEEECCCCHHHHHHHHHCC
RGFTYLVSVTGVTGERSVMEDRVQSLVQQLKLSGSNPVAVGFGISGPQQVRQVRSWGADG
CCEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHCCCCC
AIVGSALVKRMAAAAPGLVAQEAGLFCKELRNAAG
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917642