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The map label for this gene is def1

Identifier: 33862657

GI number: 33862657

Start: 433040

End: 433618

Strand: Reverse

Name: def1

Synonym: PMT0384

Alternate gene names: 33862657

Gene position: 433618-433040 (Counterclockwise)

Preceding gene: 33862658

Following gene: 33862656

Centisome position: 17.99

GC content: 50.95

Gene sequence:

>579_bases
ATGGCTGTGAAAGAAATCCTGCGTATGGGCAATCCTCAACTGAGGAAAGTGTCCAATGTCGTCGATGATGCCTCTGATGA
GCTGATCATTTCACTCATCAAGGATTTACAAGACACGGTGAAGGCCCATCAAGGAGCAGGGCTCGCCGCACCCCAAATTG
GTGTCCCTTTAAGAGTGGTGCTGTTTGGTGGAGGAGGCCCTAATCCTCGCTATCCCGAGGCCCCATCGATTCCACAAACA
CTTCTGATTAATCCTGTCTTAACACCAATCGGCTCTGATCTAGAGGATGGTTGGGAGGGGTGCTTGAGTGTGCCAGGCCT
GCGGGGCAAGGTATCGCGCTGGAGTCGGATTCACTACAGGGCGCTGAATGAAGATGGTTTTGAGGTTGAGCATTGCCTTG
AGGGCTTCCCAGCTAGAGTTATCCAACATGAATGTGACCACTTAGACGGGGTTTTGTTCCCCGATCGTCTTGTTGACTCT
GCCTCATTTGGCTTCACAGGGGAGTTAGAAACTGCTGGCATCATTGAGAAGCTTTCCAGTGCAGAGCAAAAAGCATCACA
ACAGAGCAGAGCAGATTGA

Upstream 100 bases:

>100_bases
TGATAACTCACTTGTCCATTTACAGAATCTTGCTCATCCTTGTGGCTGGTTTTGTTGTGTTGGCGAAAACTACAGAAAAG
AGAAAGGGAGGTTAATGATC

Downstream 100 bases:

>100_bases
AAGATTAAATCTTATTCTTAAAGAGACAACGCGCCATCATTAGCCAAGGGCTAAACAGTGACAATGGTCAACATGAGGCT
ATTAATATGGACATTTTTTG

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 1; Polypeptide deformylase 1

Number of amino acids: Translated: 192; Mature: 191

Protein sequence:

>192_residues
MAVKEILRMGNPQLRKVSNVVDDASDELIISLIKDLQDTVKAHQGAGLAAPQIGVPLRVVLFGGGGPNPRYPEAPSIPQT
LLINPVLTPIGSDLEDGWEGCLSVPGLRGKVSRWSRIHYRALNEDGFEVEHCLEGFPARVIQHECDHLDGVLFPDRLVDS
ASFGFTGELETAGIIEKLSSAEQKASQQSRAD

Sequences:

>Translated_192_residues
MAVKEILRMGNPQLRKVSNVVDDASDELIISLIKDLQDTVKAHQGAGLAAPQIGVPLRVVLFGGGGPNPRYPEAPSIPQT
LLINPVLTPIGSDLEDGWEGCLSVPGLRGKVSRWSRIHYRALNEDGFEVEHCLEGFPARVIQHECDHLDGVLFPDRLVDS
ASFGFTGELETAGIIEKLSSAEQKASQQSRAD
>Mature_191_residues
AVKEILRMGNPQLRKVSNVVDDASDELIISLIKDLQDTVKAHQGAGLAAPQIGVPLRVVLFGGGGPNPRYPEAPSIPQTL
LINPVLTPIGSDLEDGWEGCLSVPGLRGKVSRWSRIHYRALNEDGFEVEHCLEGFPARVIQHECDHLDGVLFPDRLVDSA
SFGFTGELETAGIIEKLSSAEQKASQQSRAD

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Homo sapiens, GI11641243, Length=169, Percent_Identity=32.5443786982249, Blast_Score=72, Evalue=2e-13,
Organism=Escherichia coli, GI1789682, Length=158, Percent_Identity=35.4430379746835, Blast_Score=79, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24645728, Length=171, Percent_Identity=32.1637426900585, Blast_Score=91, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24645726, Length=169, Percent_Identity=32.5443786982249, Blast_Score=85, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF1_PROMM (Q7V8G6)

Other databases:

- EMBL:   BX548175
- RefSeq:   NP_894217.1
- ProteinModelPortal:   Q7V8G6
- SMR:   Q7V8G6
- STRING:   Q7V8G6
- GeneID:   1728773
- GenomeReviews:   BX548175_GR
- KEGG:   pmt:PMT0384
- NMPDR:   fig|74547.1.peg.384
- eggNOG:   COG0242
- HOGENOM:   HBG665227
- OMA:   YARIRYE
- ProtClustDB:   PRK12846
- BioCyc:   PMAR74547:PMT0384-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 20788; Mature: 20657

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: ACT_SITE 144-144

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVKEILRMGNPQLRKVSNVVDDASDELIISLIKDLQDTVKAHQGAGLAAPQIGVPLRVV
CCHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEE
LFGGGGPNPRYPEAPSIPQTLLINPVLTPIGSDLEDGWEGCLSVPGLRGKVSRWSRIHYR
EECCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHH
ALNEDGFEVEHCLEGFPARVIQHECDHLDGVLFPDRLVDSASFGFTGELETAGIIEKLSS
HCCCCCCHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHH
AEQKASQQSRAD
HHHHHHHHHCCC
>Mature Secondary Structure 
AVKEILRMGNPQLRKVSNVVDDASDELIISLIKDLQDTVKAHQGAGLAAPQIGVPLRVV
CHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEE
LFGGGGPNPRYPEAPSIPQTLLINPVLTPIGSDLEDGWEGCLSVPGLRGKVSRWSRIHYR
EECCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHH
ALNEDGFEVEHCLEGFPARVIQHECDHLDGVLFPDRLVDSASFGFTGELETAGIIEKLSS
HCCCCCCHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHH
AEQKASQQSRAD
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917642