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The map label for this gene is thiE [H]

Identifier: 33862636

GI number: 33862636

Start: 409742

End: 410803

Strand: Reverse

Name: thiE [H]

Synonym: PMT0363

Alternate gene names: 33862636

Gene position: 410803-409742 (Counterclockwise)

Preceding gene: 33862637

Following gene: 33862635

Centisome position: 17.04

GC content: 54.14

Gene sequence:

>1062_bases
ATGAAATCGATGCCTTTCGCCCCGATCGCAGATCTACGTGTGGCTCAGCTGATCGATGCCAACCTCGATCGAGCTCGGGA
AGGATTGCGAGTCGTCGAAGACTGGTGCCGCTTCGGCCTAGATCGTGAAGAGCTTGTGATGACTCTCAAAGACTGGCGTC
AGCGACTGGGTCGCCATCATCACGACAGTTACAAACAGGCACGCTCCACTGCTACAGATCAAGGGATCGGCCTCAGTCAT
CCTGCTCAACAAGAGCGACACGAGCCATGGCATGTTGTGGCAGCCAACTGCGCACGCGTTCAAGAAGCTCTACGCGTGCT
TGAAGAGTTCGCCCGTCAGCCAGATCCTCAGCTGGCAGCCAGCGCTGCTGCAATCCGCTATGGCCTCTACGACCTAGAGG
TGACCGTGCTGCAGGCCAACGCAGGCAAAAAGAGACGCCAACAATTACAGGACTGCCATCTTTGCCTGATTACGACATCA
CAATCCGATCTAAATAGCAACGATCTGCTCAGGACAGTGAACGCAGCATTAGTCGCTGGCATCGACATGGTGCAATACCG
CAATAAAGAAGCTAGCGACTTGCAACGACTGACTCAGGCAAAAGAACTGGCCAGCCTATGCAGAAAGCATGGGGCGCTAT
TCATCGTTAATGACCGAATCGACTTAGCCCTTGCGGTGGACGCCGATGGCGTTCATCTCGGCCAGGACGACCTCCCCACA
GACGTAGCAAGGCGACTGATCGGCAGCGAACGACTACTGGGTCGAAGCACCCAATTCCTTGCCCAGCTTCAAAAAGCTGA
AGCAGAAGGTTGCGACTATCTAGGAGTGGGACCTGTCAACAGCACAGCCACAAAACCGGAACGACAACCAATTGGGCTTG
CCTATGTGAAGGAGGCATCTAAAGCCACCCAGCTACCTTGGTTTGCCATTGGTGGCATCAACATCTCAAACCTAGAAGCA
TTACGTCAAGCCGGAGCAAAGCGAATCGCTGTGATCGGAGCGATCATGAATTCCAAAGATCCTGCCGCTACCAGCCTTCA
ACTACTGGAGGCTCTGAGATGA

Upstream 100 bases:

>100_bases
CCACCTAAAAGAACAACCCCCCAGATCAGAACGTTCATGGTGCTATCTGAACCACTGTTGTCCATGATCGGAATGGGGAT
GCAATTGTGCCAAGACTGCC

Downstream 100 bases:

>100_bases
AACTGATGGTGAATGGTGAAATCAGGGACATCGAACCAGAACCGATGCCAACCTCGCTGGCGGCCGTGATCGAACAGCTG
GGCTACCACCCTCGCCTTGT

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MKSMPFAPIADLRVAQLIDANLDRAREGLRVVEDWCRFGLDREELVMTLKDWRQRLGRHHHDSYKQARSTATDQGIGLSH
PAQQERHEPWHVVAANCARVQEALRVLEEFARQPDPQLAASAAAIRYGLYDLEVTVLQANAGKKRRQQLQDCHLCLITTS
QSDLNSNDLLRTVNAALVAGIDMVQYRNKEASDLQRLTQAKELASLCRKHGALFIVNDRIDLALAVDADGVHLGQDDLPT
DVARRLIGSERLLGRSTQFLAQLQKAEAEGCDYLGVGPVNSTATKPERQPIGLAYVKEASKATQLPWFAIGGINISNLEA
LRQAGAKRIAVIGAIMNSKDPAATSLQLLEALR

Sequences:

>Translated_353_residues
MKSMPFAPIADLRVAQLIDANLDRAREGLRVVEDWCRFGLDREELVMTLKDWRQRLGRHHHDSYKQARSTATDQGIGLSH
PAQQERHEPWHVVAANCARVQEALRVLEEFARQPDPQLAASAAAIRYGLYDLEVTVLQANAGKKRRQQLQDCHLCLITTS
QSDLNSNDLLRTVNAALVAGIDMVQYRNKEASDLQRLTQAKELASLCRKHGALFIVNDRIDLALAVDADGVHLGQDDLPT
DVARRLIGSERLLGRSTQFLAQLQKAEAEGCDYLGVGPVNSTATKPERQPIGLAYVKEASKATQLPWFAIGGINISNLEA
LRQAGAKRIAVIGAIMNSKDPAATSLQLLEALR
>Mature_353_residues
MKSMPFAPIADLRVAQLIDANLDRAREGLRVVEDWCRFGLDREELVMTLKDWRQRLGRHHHDSYKQARSTATDQGIGLSH
PAQQERHEPWHVVAANCARVQEALRVLEEFARQPDPQLAASAAAIRYGLYDLEVTVLQANAGKKRRQQLQDCHLCLITTS
QSDLNSNDLLRTVNAALVAGIDMVQYRNKEASDLQRLTQAKELASLCRKHGALFIVNDRIDLALAVDADGVHLGQDDLPT
DVARRLIGSERLLGRSTQFLAQLQKAEAEGCDYLGVGPVNSTATKPERQPIGLAYVKEASKATQLPWFAIGGINISNLEA
LRQAGAKRIAVIGAIMNSKDPAATSLQLLEALR

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=185, Percent_Identity=30.8108108108108, Blast_Score=65, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6325042, Length=217, Percent_Identity=33.6405529953917, Blast_Score=107, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR016229
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 38987; Mature: 38987

Theoretical pI: Translated: 7.88; Mature: 7.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSMPFAPIADLRVAQLIDANLDRAREGLRVVEDWCRFGLDREELVMTLKDWRQRLGRHH
CCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC
HDSYKQARSTATDQGIGLSHPAQQERHEPWHVVAANCARVQEALRVLEEFARQPDPQLAA
CHHHHHHHHHCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
SAAAIRYGLYDLEVTVLQANAGKKRRQQLQDCHLCLITTSQSDLNSNDLLRTVNAALVAG
HHHHHHHHHEEEEEEEEECCCCHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHH
IDMVQYRNKEASDLQRLTQAKELASLCRKHGALFIVNDRIDLALAVDADGVHLGQDDLPT
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEEEEEECCCCCCCCCCCCCH
DVARRLIGSERLLGRSTQFLAQLQKAEAEGCDYLGVGPVNSTATKPERQPIGLAYVKEAS
HHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCHHHHHHHH
KATQLPWFAIGGINISNLEALRQAGAKRIAVIGAIMNSKDPAATSLQLLEALR
HHHCCCEEEECCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHC
>Mature Secondary Structure
MKSMPFAPIADLRVAQLIDANLDRAREGLRVVEDWCRFGLDREELVMTLKDWRQRLGRHH
CCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC
HDSYKQARSTATDQGIGLSHPAQQERHEPWHVVAANCARVQEALRVLEEFARQPDPQLAA
CHHHHHHHHHCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
SAAAIRYGLYDLEVTVLQANAGKKRRQQLQDCHLCLITTSQSDLNSNDLLRTVNAALVAG
HHHHHHHHHEEEEEEEEECCCCHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHH
IDMVQYRNKEASDLQRLTQAKELASLCRKHGALFIVNDRIDLALAVDADGVHLGQDDLPT
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEEEEEECCCCCCCCCCCCCH
DVARRLIGSERLLGRSTQFLAQLQKAEAEGCDYLGVGPVNSTATKPERQPIGLAYVKEAS
HHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCHHHHHHHH
KATQLPWFAIGGINISNLEALRQAGAKRIAVIGAIMNSKDPAATSLQLLEALR
HHHCCCEEEECCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA