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The map label for this gene is clpP4 [H]

Identifier: 33862588

GI number: 33862588

Start: 361312

End: 361983

Strand: Reverse

Name: clpP4 [H]

Synonym: PMT0315

Alternate gene names: 33862588

Gene position: 361983-361312 (Counterclockwise)

Preceding gene: 33862589

Following gene: 33862587

Centisome position: 15.01

GC content: 54.17

Gene sequence:

>672_bases
ATGACGGTGTCCGCTCCCTACTACGGCGAATCAACCGTGATGCGTACACCGCCTCCGGATTTGCCATCGCTACTTCTCAA
GGAACGCATTGTCTACCTAGGACTGCCCCTGTTCTCCGACGACGATGCCAAGCGGCAGCTGGGCATGGATGTGACCGAAC
TGATCATTGCCCAACTTCTCTATCTGGAGTTCGACAACCCAGAAAAACCCATCTACTTCTATATCAACTCCACTGGTACC
AGCTGGTATACGGGTGATGCCGTCGGCTTTGAAACCGAAGCTTTTGCGATCTGTGACACCCTGCGTTACGTCAAACCCCC
TGTCCACACCATCTGCATCGGCCAAGCAATGGGCACGGCAGCGGTGATTCTCTCGGCAGGAACCAAAGGCCACAGAGCTG
CCCTTCCCCACGCCTCAATCGTGCTGCACCAACCTCGCAGTGGTGCCCAAGGTCAAGCCACCGACATTCAGATCCGTGCC
AAGGAAGTGCTGCATAACAAGCAAGCCATGCTCAAAATTCTTGCCAGCAATACAGGCCGCAGCATTGAGCAGTTATCAAA
GGATTCTGATCGGATGAGTTATCTCACCCCTGGGGACGCTGTCGAATACGGCATCATCGATCGAATTCTCAGCAGCCGTA
AAGAGCTCCCCAGTTCAGAGTCACTGAACTGA

Upstream 100 bases:

>100_bases
CTTCGAGAAAAATCAGCCAACCCGCAAATCAAGTAAGACCACCGAGCACCCACCGGCTAACCCTCGCTAGTCTCCAAATT
CCGCCAAAGATCTGCTGCAG

Downstream 100 bases:

>100_bases
TCGATCCACTGCAACCCTGGACTAACCCTTCCAGGCATATCTCAGATCATTCCTAATCCAGTTCTGATTTCCTCCTTTCT
TTTTTCACCATGCCGATCGG

Product: ATP-dependent Clp protease-like protein

Products: NA

Alternate protein names: Endopeptidase Clp-like [H]

Number of amino acids: Translated: 223; Mature: 222

Protein sequence:

>223_residues
MTVSAPYYGESTVMRTPPPDLPSLLLKERIVYLGLPLFSDDDAKRQLGMDVTELIIAQLLYLEFDNPEKPIYFYINSTGT
SWYTGDAVGFETEAFAICDTLRYVKPPVHTICIGQAMGTAAVILSAGTKGHRAALPHASIVLHQPRSGAQGQATDIQIRA
KEVLHNKQAMLKILASNTGRSIEQLSKDSDRMSYLTPGDAVEYGIIDRILSSRKELPSSESLN

Sequences:

>Translated_223_residues
MTVSAPYYGESTVMRTPPPDLPSLLLKERIVYLGLPLFSDDDAKRQLGMDVTELIIAQLLYLEFDNPEKPIYFYINSTGT
SWYTGDAVGFETEAFAICDTLRYVKPPVHTICIGQAMGTAAVILSAGTKGHRAALPHASIVLHQPRSGAQGQATDIQIRA
KEVLHNKQAMLKILASNTGRSIEQLSKDSDRMSYLTPGDAVEYGIIDRILSSRKELPSSESLN
>Mature_222_residues
TVSAPYYGESTVMRTPPPDLPSLLLKERIVYLGLPLFSDDDAKRQLGMDVTELIIAQLLYLEFDNPEKPIYFYINSTGTS
WYTGDAVGFETEAFAICDTLRYVKPPVHTICIGQAMGTAAVILSAGTKGHRAALPHASIVLHQPRSGAQGQATDIQIRAK
EVLHNKQAMLKILASNTGRSIEQLSKDSDRMSYLTPGDAVEYGIIDRILSSRKELPSSESLN

Specific function: Has lost the two conserved residues (Ser and His) proposed to be part of the active site. Therefore it could be inactive [H]

COG id: COG0740

COG function: function code OU; Protease subunit of ATP-dependent Clp proteases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S14 family [H]

Homologues:

Organism=Homo sapiens, GI5174419, Length=191, Percent_Identity=40.3141361256544, Blast_Score=143, Evalue=1e-34,
Organism=Escherichia coli, GI1786641, Length=195, Percent_Identity=43.5897435897436, Blast_Score=166, Evalue=9e-43,
Organism=Caenorhabditis elegans, GI17538017, Length=199, Percent_Identity=37.6884422110553, Blast_Score=132, Evalue=1e-31,
Organism=Drosophila melanogaster, GI20129427, Length=191, Percent_Identity=40.8376963350785, Blast_Score=143, Evalue=7e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001907 [H]

Pfam domain/function: PF00574 CLP_protease [H]

EC number: 3.4.21.92

Molecular weight: Translated: 24487; Mature: 24356

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVSAPYYGESTVMRTPPPDLPSLLLKERIVYLGLPLFSDDDAKRQLGMDVTELIIAQLL
CCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHH
YLEFDNPEKPIYFYINSTGTSWYTGDAVGFETEAFAICDTLRYVKPPVHTICIGQAMGTA
HHCCCCCCCCEEEEEECCCCCEEECCCCCCCCHHHHHHHHHHHHCCCHHEEEECHHHCCE
AVILSAGTKGHRAALPHASIVLHQPRSGAQGQATDIQIRAKEVLHNKQAMLKILASNTGR
EEEEECCCCCCEECCCCCEEEEECCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHCCCCH
SIEQLSKDSDRMSYLTPGDAVEYGIIDRILSSRKELPSSESLN
HHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TVSAPYYGESTVMRTPPPDLPSLLLKERIVYLGLPLFSDDDAKRQLGMDVTELIIAQLL
CCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHH
YLEFDNPEKPIYFYINSTGTSWYTGDAVGFETEAFAICDTLRYVKPPVHTICIGQAMGTA
HHCCCCCCCCEEEEEECCCCCEEECCCCCCCCHHHHHHHHHHHHCCCHHEEEECHHHCCE
AVILSAGTKGHRAALPHASIVLHQPRSGAQGQATDIQIRAKEVLHNKQAMLKILASNTGR
EEEEECCCCCCEECCCCCEEEEECCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHCCCCH
SIEQLSKDSDRMSYLTPGDAVEYGIIDRILSSRKELPSSESLN
HHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: ATP imidodiphosphate; dATP degradation, [C]

Metal ions: Ca2+; Mg2+; Mn2+; Zn2+ [C]

Kcat value (1/min): 800 [C]

Specific activity: NA

Km value (mM): 0.21 {ATP}} 1.3 {N-succinyl-Leu-Tyr} [C]

Substrates: NA

Specific reaction: NA

General reaction: Hydrolysis of peptide bonds [C]

Inhibitor: Diisopropyl fluorophosphate ClpAP; Fluorosulfonyl benzoyl adenosine; Kappa-Casein; Mg2+; NEM hydrolysis; Neohydrin; Succinyl -Leu-Tyr4-methyl coumarin7-amide; Xaa-Tyr-Leu-Tyr-Trp [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]