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The map label for this gene is res [H]

Identifier: 33862567

GI number: 33862567

Start: 333292

End: 333927

Strand: Reverse

Name: res [H]

Synonym: PMT0294

Alternate gene names: 33862567

Gene position: 333927-333292 (Counterclockwise)

Preceding gene: 33862570

Following gene: 33862566

Centisome position: 13.85

GC content: 49.37

Gene sequence:

>636_bases
ATGGGCAAATTCCAAAAGCGCAAGTTCGAGAGACCATCATTCATGGATAAACCCAACTACAAATCGATTGGTTATGCCCG
CGTCAGTACCTCAGGGCAGACCACTGAAGGTCAGGTTGTTGATCTGAAAAAAGAAGGGTGCTGTGTTGTTTTCCAGGAAA
CGATCAGCACAACAGTCAAAGAGAAAGAACGACCTCAACTGATGGCAGCACTCAGTGCTCTTGATGAAGGTGATGAGTTG
GTGGTCTCGAAGATGGATCGATTAGGTAGGACCCAGGTCGAGGTGGTTAATCGCATCCATGACCTGCAGCAGCAAGGCAT
CCATATCAGGACCTTGGATGGACTGATCAATACAAGAGGACTTGGGAAATTCGCTCCTGTGCTCATTGGTCTGCTCTCTG
GTCTTGCAGAAGTAGAGCGCTCACTGACCAGAGAGAGAACGATTGAAAGCATCCAACACCGCAAAGACACTGGCGGGAAC
TTGGGAGGTAGACCCAAGACCAGTCGCATCAAAGAAGGTCTTGTCCTACGCCTAAGAAAGGAAGGGTGCTCTTACAGGTC
CATAAGAGAGCAAACGGGTCTTGCTCTATCGACGATTAGAAGGATCATCGTGGAGCAAGAGGTCGTGACGGCATGA

Upstream 100 bases:

>100_bases
CCTCAAAATCCAGTGAAGTCAATCTCAGATGTGTTCCATTAAGGCGTTCTGGTTGCTAAAGTAGAAATACAGGTGAAACA
CCCCTCTTTTTGGAACACGC

Downstream 100 bases:

>100_bases
TCAAAACTCTTCTACTTGCTGTCATCGTTGCCCTTCTGTGGTCTACACCTCAAGCAAGAGAGACAACAGCAAATGCACTA
CGAATTGCGGCAAACTGGTT

Product: site-specific recombinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 211; Mature: 210

Protein sequence:

>211_residues
MGKFQKRKFERPSFMDKPNYKSIGYARVSTSGQTTEGQVVDLKKEGCCVVFQETISTTVKEKERPQLMAALSALDEGDEL
VVSKMDRLGRTQVEVVNRIHDLQQQGIHIRTLDGLINTRGLGKFAPVLIGLLSGLAEVERSLTRERTIESIQHRKDTGGN
LGGRPKTSRIKEGLVLRLRKEGCSYRSIREQTGLALSTIRRIIVEQEVVTA

Sequences:

>Translated_211_residues
MGKFQKRKFERPSFMDKPNYKSIGYARVSTSGQTTEGQVVDLKKEGCCVVFQETISTTVKEKERPQLMAALSALDEGDEL
VVSKMDRLGRTQVEVVNRIHDLQQQGIHIRTLDGLINTRGLGKFAPVLIGLLSGLAEVERSLTRERTIESIQHRKDTGGN
LGGRPKTSRIKEGLVLRLRKEGCSYRSIREQTGLALSTIRRIIVEQEVVTA
>Mature_210_residues
GKFQKRKFERPSFMDKPNYKSIGYARVSTSGQTTEGQVVDLKKEGCCVVFQETISTTVKEKERPQLMAALSALDEGDELV
VSKMDRLGRTQVEVVNRIHDLQQQGIHIRTLDGLINTRGLGKFAPVLIGLLSGLAEVERSLTRERTIESIQHRKDTGGNL
GGRPKTSRIKEGLVLRLRKEGCSYRSIREQTGLALSTIRRIIVEQEVVTA

Specific function: A likely role for the res protein would be to stabilize pCP13 by reducing the number of plasmid multimers resulting from homologous recombination [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=145, Percent_Identity=40, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1787638, Length=185, Percent_Identity=30.8108108108108, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI1787827, Length=185, Percent_Identity=30.8108108108108, Blast_Score=72, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR006118
- InterPro:   IPR006119
- InterPro:   IPR006120 [H]

Pfam domain/function: PF02796 HTH_7; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 23609; Mature: 23477

Theoretical pI: Translated: 10.36; Mature: 10.36

Prosite motif: PS00397 RECOMBINASES_1 ; PS00398 RECOMBINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKFQKRKFERPSFMDKPNYKSIGYARVSTSGQTTEGQVVDLKKEGCCVVFQETISTTVK
CCCCHHHHCCCCCCCCCCCCCCCCEEEECCCCCCCCCCEEEECCCCCEEEHHHHHHHHHH
EKERPQLMAALSALDEGDELVVSKMDRLGRTQVEVVNRIHDLQQQGIHIRTLDGLINTRG
HHHHHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCEEEEHHHHHHCCC
LGKFAPVLIGLLSGLAEVERSLTRERTIESIQHRKDTGGNLGGRPKTSRIKEGLVLRLRK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHH
EGCSYRSIREQTGLALSTIRRIIVEQEVVTA
HCCCHHHHHHHHCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GKFQKRKFERPSFMDKPNYKSIGYARVSTSGQTTEGQVVDLKKEGCCVVFQETISTTVK
CCCHHHHCCCCCCCCCCCCCCCCEEEECCCCCCCCCCEEEECCCCCEEEHHHHHHHHHH
EKERPQLMAALSALDEGDELVVSKMDRLGRTQVEVVNRIHDLQQQGIHIRTLDGLINTRG
HHHHHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCEEEEHHHHHHCCC
LGKFAPVLIGLLSGLAEVERSLTRERTIESIQHRKDTGGNLGGRPKTSRIKEGLVLRLRK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHH
EGCSYRSIREQTGLALSTIRRIIVEQEVVTA
HCCCHHHHHHHHCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11792842 [H]