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The map label for this gene is intA [H]

Identifier: 33862563

GI number: 33862563

Start: 329289

End: 330557

Strand: Direct

Name: intA [H]

Synonym: PMT0290

Alternate gene names: 33862563

Gene position: 329289-330557 (Clockwise)

Preceding gene: 33862562

Following gene: 33862565

Centisome position: 13.66

GC content: 46.97

Gene sequence:

>1269_bases
ATGGGTCTGACAGAAAGGGAAGTCAGGTCACTATCAGTCAAGTCAAAGAGGTACAGCAGGTCCGTGGGAGACTCCCTATA
TGTATTAGTAGAACCTGTAAAAGGTAGTGGTGGCAAGAGTTTTGTAGGGAAAATGCGTTCTAATGGCAAGCAAATTGATG
TTCGTATCGGCGTTTTTGGCAAAGGTCCAGGGCAGTGGACTCTCAAAGCAGCAAGAGATGAATGGTTTCGTTTGCGGAGT
TGGTATAGAGAGACAGGCAGAGATCCAAGAGAATTGCAAAAGGATGAGAAAAGGCAATTAGCAAAACAATCCTCTGATCA
AACACTTAATGAGTTGGTTGATTCTTATTTGATTGCATGTAAGACGAAATACAAACAATCAGAGACCACCATTAAGGACA
AGAAAAATAAGTTGTCCAATGAAATTATCCCTGCATTAGGTGGAAGTACTCCTGTTAGTCGTTTGGGATGGGATGAGAAA
TCAAAAGGTAGGACTGGCAGAGAAGCGGTCTTTGAGATCACTGACTCCATAGAGAAGAGGGGGTCTCTAAATCAGGCAAG
GAAGGTGCTTGGTGTGATGAGGCACCTCTTTGACTACGCCATAGAACGGGGATGGATGGAGAGAGGTCAAAACCCTGCAG
TGCAAGCAAGGAGTGTTGGTGCAGGGCATGAGGTCCAACACAACCCTTGTTTGTCGTGGGATGAGTTGCCGCAGTTATTG
GATGACCTAAACCGCAATAAGGGGAATGGGTCACAGGTGGTTCTTGCTGCGATCAAGTTGGATTTAATGACTTTCTTGCG
TGTTGGGTCATTGGTTCCAATGCGTTGGGATGAAGTGGATTACGCAGCAAACCTATGGACTGTGCCTGGATCCAGGATGA
AGAGTGGCAAGAATCATTGGGTTCCTCTGACTGATCCAATTAAGGATCTGCTGGCACATCTGAGGAAAATCAATGGTGAC
CAGGATTATGTGTTCTGGAGTCCAAGGGGTAGAAGCAAACCCCATATCGATGAGTCAGCACTGAACCAACACCTCAAAGT
GCGCCTTGGTTATGGAGGAAGGCAAACCGCTCATGGACTTCGTTCTTTGCCACTAACAGCAGGGCAGGAAGTCCTCAAGT
TCTCTGCTGAGGTCATTCAACGGCAGATGGACCATGCAGTTGGGGACAAGGTTCGTCAGGCATACGACAAGTCCCAACTT
CTTGATGAGAGGTGGAAGTTCATGCGTGCCTGGTGTGATGCTCTCCTGGATCAAGGACTTGAGGTTTGA

Upstream 100 bases:

>100_bases
CTCGGAAAACCCCTCCGAAGCGCTTGATTTGTTGGTGGATATGTTGGTGGATATTGTGGTTGTGTTGGTGGATAAAATGT
ATGTTGGTGGATTTCGTTAG

Downstream 100 bases:

>100_bases
GAAGGGGCATCAAGCAGTTCCATTGGTCGAGGAACCCTTCTTGCACCTGCTCTTAATTGGCAACCAGTCTTCTGAGTAGG
TACTGGTCCATCTCGCCTTA

Product: Phage integrase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 422; Mature: 421

Protein sequence:

>422_residues
MGLTEREVRSLSVKSKRYSRSVGDSLYVLVEPVKGSGGKSFVGKMRSNGKQIDVRIGVFGKGPGQWTLKAARDEWFRLRS
WYRETGRDPRELQKDEKRQLAKQSSDQTLNELVDSYLIACKTKYKQSETTIKDKKNKLSNEIIPALGGSTPVSRLGWDEK
SKGRTGREAVFEITDSIEKRGSLNQARKVLGVMRHLFDYAIERGWMERGQNPAVQARSVGAGHEVQHNPCLSWDELPQLL
DDLNRNKGNGSQVVLAAIKLDLMTFLRVGSLVPMRWDEVDYAANLWTVPGSRMKSGKNHWVPLTDPIKDLLAHLRKINGD
QDYVFWSPRGRSKPHIDESALNQHLKVRLGYGGRQTAHGLRSLPLTAGQEVLKFSAEVIQRQMDHAVGDKVRQAYDKSQL
LDERWKFMRAWCDALLDQGLEV

Sequences:

>Translated_422_residues
MGLTEREVRSLSVKSKRYSRSVGDSLYVLVEPVKGSGGKSFVGKMRSNGKQIDVRIGVFGKGPGQWTLKAARDEWFRLRS
WYRETGRDPRELQKDEKRQLAKQSSDQTLNELVDSYLIACKTKYKQSETTIKDKKNKLSNEIIPALGGSTPVSRLGWDEK
SKGRTGREAVFEITDSIEKRGSLNQARKVLGVMRHLFDYAIERGWMERGQNPAVQARSVGAGHEVQHNPCLSWDELPQLL
DDLNRNKGNGSQVVLAAIKLDLMTFLRVGSLVPMRWDEVDYAANLWTVPGSRMKSGKNHWVPLTDPIKDLLAHLRKINGD
QDYVFWSPRGRSKPHIDESALNQHLKVRLGYGGRQTAHGLRSLPLTAGQEVLKFSAEVIQRQMDHAVGDKVRQAYDKSQL
LDERWKFMRAWCDALLDQGLEV
>Mature_421_residues
GLTEREVRSLSVKSKRYSRSVGDSLYVLVEPVKGSGGKSFVGKMRSNGKQIDVRIGVFGKGPGQWTLKAARDEWFRLRSW
YRETGRDPRELQKDEKRQLAKQSSDQTLNELVDSYLIACKTKYKQSETTIKDKKNKLSNEIIPALGGSTPVSRLGWDEKS
KGRTGREAVFEITDSIEKRGSLNQARKVLGVMRHLFDYAIERGWMERGQNPAVQARSVGAGHEVQHNPCLSWDELPQLLD
DLNRNKGNGSQVVLAAIKLDLMTFLRVGSLVPMRWDEVDYAANLWTVPGSRMKSGKNHWVPLTDPIKDLLAHLRKINGDQ
DYVFWSPRGRSKPHIDESALNQHLKVRLGYGGRQTAHGLRSLPLTAGQEVLKFSAEVIQRQMDHAVGDKVRQAYDKSQLL
DERWKFMRAWCDALLDQGLEV

Specific function: Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome [H]

COG id: COG0582

COG function: function code L; Integrase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI1788974, Length=420, Percent_Identity=28.0952380952381, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1788690, Length=424, Percent_Identity=27.8301886792453, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI145693166, Length=415, Percent_Identity=25.3012048192771, Blast_Score=83, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 47865; Mature: 47734

Theoretical pI: Translated: 10.28; Mature: 10.28

Prosite motif: PS00142 ZINC_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLTEREVRSLSVKSKRYSRSVGDSLYVLVEPVKGSGGKSFVGKMRSNGKQIDVRIGVFG
CCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHCCCCEEEEEEEEEE
KGPGQWTLKAARDEWFRLRSWYRETGRDPRELQKDEKRQLAKQSSDQTLNELVDSYLIAC
CCCCCEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
KTKYKQSETTIKDKKNKLSNEIIPALGGSTPVSRLGWDEKSKGRTGREAVFEITDSIEKR
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHC
GSLNQARKVLGVMRHLFDYAIERGWMERGQNPAVQARSVGAGHEVQHNPCLSWDELPQLL
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHH
DDLNRNKGNGSQVVLAAIKLDLMTFLRVGSLVPMRWDEVDYAANLWTVPGSRMKSGKNHW
HHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHEEECCCHHHHCCCCCC
VPLTDPIKDLLAHLRKINGDQDYVFWSPRGRSKPHIDESALNQHLKVRLGYGGRQTAHGL
CCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCHHHHCCCEEEEECCCCCHHHHHH
RSLPLTAGQEVLKFSAEVIQRQMDHAVGDKVRQAYDKSQLLDERWKFMRAWCDALLDQGL
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EV
CC
>Mature Secondary Structure 
GLTEREVRSLSVKSKRYSRSVGDSLYVLVEPVKGSGGKSFVGKMRSNGKQIDVRIGVFG
CCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHCCCCEEEEEEEEEE
KGPGQWTLKAARDEWFRLRSWYRETGRDPRELQKDEKRQLAKQSSDQTLNELVDSYLIAC
CCCCCEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
KTKYKQSETTIKDKKNKLSNEIIPALGGSTPVSRLGWDEKSKGRTGREAVFEITDSIEKR
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHC
GSLNQARKVLGVMRHLFDYAIERGWMERGQNPAVQARSVGAGHEVQHNPCLSWDELPQLL
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHH
DDLNRNKGNGSQVVLAAIKLDLMTFLRVGSLVPMRWDEVDYAANLWTVPGSRMKSGKNHW
HHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHEEECCCHHHHCCCCCC
VPLTDPIKDLLAHLRKINGDQDYVFWSPRGRSKPHIDESALNQHLKVRLGYGGRQTAHGL
CCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCHHHHCCCEEEEECCCCCHHHHHH
RSLPLTAGQEVLKFSAEVIQRQMDHAVGDKVRQAYDKSQLLDERWKFMRAWCDALLDQGL
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EV
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7511583; 9278503; 9205837; 7524073 [H]