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The map label for this gene is gspE1 [H]

Identifier: 33862293

GI number: 33862293

Start: 23030

End: 24946

Strand: Reverse

Name: gspE1 [H]

Synonym: PMT0020

Alternate gene names: 33862293

Gene position: 24946-23030 (Counterclockwise)

Preceding gene: 33862298

Following gene: 33862292

Centisome position: 1.03

GC content: 52.84

Gene sequence:

>1917_bases
ATGCTGACCGTTTGCAAATTCACGTCTAGACATGTTGATGGGAATAGCACACCTCCCACAAGCGGCGGAAGCCCGCACCT
CCAATTTCATTTCTTCTTCGTGACCCAGGGCCGACCAATCCCCAAAGCAACCAACAGCATCCAGCAGCGACTGGAACTGG
AACTGCTCCTACAAGTCAATGTGCTTAGCCACGAAGAACTTGTTGTCGGAGTGGAGCTAATGGCCAACCACACCACTCTT
GACATCTCAACGTGGCAACAATTCCAGGCCCTGCCAATCAACATGCACAACCAACATTTGGTTGTAGCCATCTCGGACCA
ATGCAATGAGCAAACCAAAAATCAACTGATCTCAGTGCTGCAGTCCCAAGGCTTCAGCACAGAATTCCGTCTCGCGCTTG
CAGCCGACATCAGCCAGCTGCTTGCACCAATGAGATCTGAGCAGCACGTTGAATCTGCATCCAAGTCAAAAACAACAAAG
CCAATTGCACAAACACCAACCTCCCTGCTGGCAGGATTTAGTGCCGAAGGCGTGCTCGAAGAAGATCCTGAAGAACAAGC
CAGACTTGCTAGTTCCATAGAGGATTTGGAGTCCAGCTTGATGGACTCAGACAGTTCACCAGTCATCAACCTGGTGGACC
GCATATTGCTAGAGGCACTGCAAACAGAAGCCAGCGACGTACACGTTGAGCCGCAACAAGACGGACTACAGATCCGCTTC
CGTCAAGACGGTGTCCTGCAGCGCTATATCGAACCCCTCCCGAGCCGGTTGATCCCTGCTGTTACCTCACGCTTCAAGAT
CATGGCCGACCTAGACATCGCAGAACGGCGCATGGCTCAGGACGGTCGCATTCGTCGCACATATCGCAATCGTATGGTCG
ATTTCAGGGTTAATAGCCTGCCAAGCCGTTACGGGGAAAAAATCTGTCTGCGACTACTCGACAGCAGTGCTCCACAACTC
GGGTTAGACAAACTGATCAGCAACCCAAGTGCTCTCTCTCTCGTACGCAACTTGGGCTCTAAGCCCTTCGGCATGATCCT
TGTGACAGGTCCAACAGGGTCAGGCAAGTCAACAACCCTTTACTCCCTCCTGGCAGAACGCAACGAACCTGGTATCAACA
TTTCCACCGTAGAAGATCCCATCGAATACACCCTCCCTGGGATTACCCAATGTCAAGTGAATAGGGAAAAAGGCTTCGAT
TTCAGCACTGCACTGAGAGCCTTCATGCGTCAAGACCCAGACGTTCTGCTGGTCGGTGAAACCCGTGACCTAGAAACCGC
AAAAACCGCCATCGAAGCCGCACTGACTGGTCATCTGGTACTAACCACACTGCATTGCAATGACGCATCAAGTGCGATCG
CCCGCCTCAACGAGATGGGCGTGGAACCATTCATGGTGAGCGCTTCGTTGATTGGCATCGTCTCTCAACGACTTCTACGA
AGAGTGTGCCGTTCTTGTCGTAAGTCATATCACCCAACTGAAAAAGAACTGGGGCGATTCGGCTTGATGGCCCATACAGA
AACTGGGGTGACCTTTTTCAAGGCCCACCATCACGGTCAAGAAAAACAGCCGTGCCCCAAATGTCAAGGCAGCGGCTACA
AGGGCCGAGTTGGTGTCTATGAAGTACTGCGCATGAACGAAGAGCTCGCTTCAGCCGTCGCCAAAGGTGCCACTACTGAT
TTAGTAAGACGTCTGGCGCTCGAGGCTGGTATGAAAACTCTATTGGGCTACAGCCTTGACCTGGTGCGAGAAGGCCACAC
CACCCTTGAGGAAGTAGGCCGAATGATCCTCACCGATTCCGGATTGGAATCAGAGCGCCGCGCCAGAGCCCTTAGCACTC
TTACCTGCAACGTCTGCGGGGCAGGCCTCCAAGACGACTGGCTGGAATGTCCCTACTGTCTAACCGCTCGCCAATGA

Upstream 100 bases:

>100_bases
ACCCTCGTCGGAATTGATCGATGCCACTGATTCCAGAGGCACGGAAGCCTCTAAACCCTCAGCAGCAGGATCGTGAGCTG
GGGTAGGGGCGTCGCCACTC

Downstream 100 bases:

>100_bases
AGGACCCGTCATGGATCTAATGATCGAGGACCTGATGGAACAGCTGGTGCAAGGTGGCGGCAGTGATCTGCACATTGCTA
CCGGCCAACCCCCGTATGGA

Product: general secretion pathway protein E

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 638; Mature: 638

Protein sequence:

>638_residues
MLTVCKFTSRHVDGNSTPPTSGGSPHLQFHFFFVTQGRPIPKATNSIQQRLELELLLQVNVLSHEELVVGVELMANHTTL
DISTWQQFQALPINMHNQHLVVAISDQCNEQTKNQLISVLQSQGFSTEFRLALAADISQLLAPMRSEQHVESASKSKTTK
PIAQTPTSLLAGFSAEGVLEEDPEEQARLASSIEDLESSLMDSDSSPVINLVDRILLEALQTEASDVHVEPQQDGLQIRF
RQDGVLQRYIEPLPSRLIPAVTSRFKIMADLDIAERRMAQDGRIRRTYRNRMVDFRVNSLPSRYGEKICLRLLDSSAPQL
GLDKLISNPSALSLVRNLGSKPFGMILVTGPTGSGKSTTLYSLLAERNEPGINISTVEDPIEYTLPGITQCQVNREKGFD
FSTALRAFMRQDPDVLLVGETRDLETAKTAIEAALTGHLVLTTLHCNDASSAIARLNEMGVEPFMVSASLIGIVSQRLLR
RVCRSCRKSYHPTEKELGRFGLMAHTETGVTFFKAHHHGQEKQPCPKCQGSGYKGRVGVYEVLRMNEELASAVAKGATTD
LVRRLALEAGMKTLLGYSLDLVREGHTTLEEVGRMILTDSGLESERRARALSTLTCNVCGAGLQDDWLECPYCLTARQ

Sequences:

>Translated_638_residues
MLTVCKFTSRHVDGNSTPPTSGGSPHLQFHFFFVTQGRPIPKATNSIQQRLELELLLQVNVLSHEELVVGVELMANHTTL
DISTWQQFQALPINMHNQHLVVAISDQCNEQTKNQLISVLQSQGFSTEFRLALAADISQLLAPMRSEQHVESASKSKTTK
PIAQTPTSLLAGFSAEGVLEEDPEEQARLASSIEDLESSLMDSDSSPVINLVDRILLEALQTEASDVHVEPQQDGLQIRF
RQDGVLQRYIEPLPSRLIPAVTSRFKIMADLDIAERRMAQDGRIRRTYRNRMVDFRVNSLPSRYGEKICLRLLDSSAPQL
GLDKLISNPSALSLVRNLGSKPFGMILVTGPTGSGKSTTLYSLLAERNEPGINISTVEDPIEYTLPGITQCQVNREKGFD
FSTALRAFMRQDPDVLLVGETRDLETAKTAIEAALTGHLVLTTLHCNDASSAIARLNEMGVEPFMVSASLIGIVSQRLLR
RVCRSCRKSYHPTEKELGRFGLMAHTETGVTFFKAHHHGQEKQPCPKCQGSGYKGRVGVYEVLRMNEELASAVAKGATTD
LVRRLALEAGMKTLLGYSLDLVREGHTTLEEVGRMILTDSGLESERRARALSTLTCNVCGAGLQDDWLECPYCLTARQ
>Mature_638_residues
MLTVCKFTSRHVDGNSTPPTSGGSPHLQFHFFFVTQGRPIPKATNSIQQRLELELLLQVNVLSHEELVVGVELMANHTTL
DISTWQQFQALPINMHNQHLVVAISDQCNEQTKNQLISVLQSQGFSTEFRLALAADISQLLAPMRSEQHVESASKSKTTK
PIAQTPTSLLAGFSAEGVLEEDPEEQARLASSIEDLESSLMDSDSSPVINLVDRILLEALQTEASDVHVEPQQDGLQIRF
RQDGVLQRYIEPLPSRLIPAVTSRFKIMADLDIAERRMAQDGRIRRTYRNRMVDFRVNSLPSRYGEKICLRLLDSSAPQL
GLDKLISNPSALSLVRNLGSKPFGMILVTGPTGSGKSTTLYSLLAERNEPGINISTVEDPIEYTLPGITQCQVNREKGFD
FSTALRAFMRQDPDVLLVGETRDLETAKTAIEAALTGHLVLTTLHCNDASSAIARLNEMGVEPFMVSASLIGIVSQRLLR
RVCRSCRKSYHPTEKELGRFGLMAHTETGVTFFKAHHHGQEKQPCPKCQGSGYKGRVGVYEVLRMNEELASAVAKGATTD
LVRRLALEAGMKTLLGYSLDLVREGHTTLEEVGRMILTDSGLESERRARALSTLTCNVCGAGLQDDWLECPYCLTARQ

Specific function: Involved in the translocation of the type IV pilin [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=460, Percent_Identity=43.9130434782609, Blast_Score=341, Evalue=8e-95,
Organism=Escherichia coli, GI1786296, Length=390, Percent_Identity=38.7179487179487, Blast_Score=266, Evalue=4e-72,
Organism=Escherichia coli, GI87082188, Length=199, Percent_Identity=40.7035175879397, Blast_Score=116, Evalue=5e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013374
- InterPro:   IPR007831
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E; PF05157 GSPII_E_N [H]

EC number: NA

Molecular weight: Translated: 70506; Mature: 70506

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTVCKFTSRHVDGNSTPPTSGGSPHLQFHFFFVTQGRPIPKATNSIQQRLELELLLQVN
CCEEEECHHCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHEEEEE
VLSHEELVVGVELMANHTTLDISTWQQFQALPINMHNQHLVVAISDQCNEQTKNQLISVL
CCCCCHHEEEHHHHCCCCEEEHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHH
QSQGFSTEFRLALAADISQLLAPMRSEQHVESASKSKTTKPIAQTPTSLLAGFSAEGVLE
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCHHHHHCCCCCCCCC
EDPEEQARLASSIEDLESSLMDSDSSPVINLVDRILLEALQTEASDVHVEPQQDGLQIRF
CCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCEEECCCCCCCEEEE
RQDGVLQRYIEPLPSRLIPAVTSRFKIMADLDIAERRMAQDGRIRRTYRNRMVDFRVNSL
CCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHCCCHHHHHHHHHHEEEEHHHC
PSRYGEKICLRLLDSSAPQLGLDKLISNPSALSLVRNLGSKPFGMILVTGPTGSGKSTTL
HHHHHHHHHHHHHHCCCCCCCHHHHHCCCHHHHHHHHCCCCCCEEEEEECCCCCCCHHHH
YSLLAERNEPGINISTVEDPIEYTLPGITQCQVNREKGFDFSTALRAFMRQDPDVLLVGE
HHHHHHCCCCCCEEEECCCCHHHCCCCCEEEEECHHCCCCHHHHHHHHHHCCCCEEEEEC
TRDLETAKTAIEAALTGHLVLTTLHCNDASSAIARLNEMGVEPFMVSASLIGIVSQRLLR
CCCHHHHHHHHHHHHCCEEEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
RVCRSCRKSYHPTEKELGRFGLMAHTETGVTFFKAHHHGQEKQPCPKCQGSGYKGRVGVY
HHHHHHHHCCCCCHHHHHHHCCEEECCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHH
EVLRMNEELASAVAKGATTDLVRRLALEAGMKTLLGYSLDLVREGHTTLEEVGRMILTDS
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHEECCC
GLESERRARALSTLTCNVCGAGLQDDWLECPYCLTARQ
CCCHHHHHHHHHHHHHHHCCCCCCCCHHCCCCEECCCC
>Mature Secondary Structure
MLTVCKFTSRHVDGNSTPPTSGGSPHLQFHFFFVTQGRPIPKATNSIQQRLELELLLQVN
CCEEEECHHCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHEEEEE
VLSHEELVVGVELMANHTTLDISTWQQFQALPINMHNQHLVVAISDQCNEQTKNQLISVL
CCCCCHHEEEHHHHCCCCEEEHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHH
QSQGFSTEFRLALAADISQLLAPMRSEQHVESASKSKTTKPIAQTPTSLLAGFSAEGVLE
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCHHHHHCCCCCCCCC
EDPEEQARLASSIEDLESSLMDSDSSPVINLVDRILLEALQTEASDVHVEPQQDGLQIRF
CCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCEEECCCCCCCEEEE
RQDGVLQRYIEPLPSRLIPAVTSRFKIMADLDIAERRMAQDGRIRRTYRNRMVDFRVNSL
CCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHCCCHHHHHHHHHHEEEEHHHC
PSRYGEKICLRLLDSSAPQLGLDKLISNPSALSLVRNLGSKPFGMILVTGPTGSGKSTTL
HHHHHHHHHHHHHHCCCCCCCHHHHHCCCHHHHHHHHCCCCCCEEEEEECCCCCCCHHHH
YSLLAERNEPGINISTVEDPIEYTLPGITQCQVNREKGFDFSTALRAFMRQDPDVLLVGE
HHHHHHCCCCCCEEEECCCCHHHCCCCCEEEEECHHCCCCHHHHHHHHHHCCCCEEEEEC
TRDLETAKTAIEAALTGHLVLTTLHCNDASSAIARLNEMGVEPFMVSASLIGIVSQRLLR
CCCHHHHHHHHHHHHCCEEEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
RVCRSCRKSYHPTEKELGRFGLMAHTETGVTFFKAHHHGQEKQPCPKCQGSGYKGRVGVY
HHHHHHHHCCCCCHHHHHHHCCEEECCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHH
EVLRMNEELASAVAKGATTDLVRRLALEAGMKTLLGYSLDLVREGHTTLEEVGRMILTDS
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHEECCC
GLESERRARALSTLTCNVCGAGLQDDWLECPYCLTARQ
CCCHHHHHHHHHHHHHHHCCCCCCCCHHCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8820654 [H]