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The map label for this gene is pleD [H]

Identifier: 326424077

GI number: 326424077

Start: 2866268

End: 2867596

Strand: Reverse

Name: pleD [H]

Synonym: NA

Alternate gene names: 326424077

Gene position: 2867596-2866268 (Counterclockwise)

Preceding gene: 27366091

Following gene: 27366084

Centisome position: 87.38

GC content: 46.58

Gene sequence:

>1329_bases
ATGAAATGGATACATAAACTTATTTTTGCTCTCGCAATATGTACCATCAGTTTAGTCGCGCTTTATAGTGTGTTAGGTGA
CACGAGAAAACTTGTCATCACACCAGAACGTTTTGCTTATTTTGCCACCGATGATCGGGCAGCAGGCGGTATTACGACGA
CGGAAATGGCGATTGATGGCCAAACGATAGATTTAAGCTGTAACATGCAAACGGCTAACTACCCGTGGCCTTACTGTGGC
ATTTCGATTTTTACTGACACACTTGAAGCGACTAAAGGTATCGACCTTTCCAACTACCACACCATCCGGCTCAAACTCAG
CTACGACAAAACGGGCGAATCCAATGGTCGATTACGCTTCTATTTGCGCAATTTTAATCCGGCTTATTCCACCGCAGATA
ATGAGTACACTTACAAATACAACGGCATGGAGTTCACACCGAGTGCCAGCATGGAAACCATTGAGATCCCGTTGGCGAAC
TTGCAAGTCATGACCTGGTGGCTAGCCGACAATAAGATTCCAATTGAACATTCTGCACCAGAATATTCCAACGTGACACG
AATTGAGTTGGCGACAGGCTCAGGAGCGGCAAATGGAGAGCATCATATCCGTATTGAACGCATTGAGTTTGAAGGTGCGT
ATTTAGCTCAGGAAACCCTGTTGTTTGCTTTGTTGCTTTCTTGGGTATTATTGGGACTGGCGTTTTCTTTGAATGAACTA
CGTAAAAGTAAGCACACCCTAGCTGAGTCTCGTTTTCGCCAAGAGCATTTAAAGAAGATGAATGCGGATCTTCGCGCGCA
GAATTACGAGTTTGCTGAACTTGCTCACCGCGATGCGTTGACGGGAGCGATGAACCGTCATGCGGTTCGAACTTGGTTGG
AGAAAAAAGCGCGTACGGTACGTTGGGGGCAAGAACAGCTTTCAGTTCTCTATCTGGATCTGGATAAGTTCAAGCGTGTC
AATGACCGTTATGGCCATCAAATGGGAGACGACATCTTACGTGAATTCGTGCTGGTGGTCGCCAGTTCTTTAGAAGAAGG
TGAACGCTTAGTTCGATGGGGCGGTGAGGAGTTTATCGTCTTTCTTCCAGAAACCACGCTGCAACAAGCAGTAGAAAAAG
CAGAAATGATTCGTAAAAATGTTTCGAATCATCTTTGGGTGCATGGTGGCATGCTTACTTGCAGTATCGGCGTGGCGCAG
ATGCAGGAAGAACGGGTAACGGAGTTTCTTGCTCGTGCTGATGACGCACTCTATCAAGCCAAACATCGCGGCCGTAACCG
AGTCGAAGTGAATTACGGTATGCAGAAGATAACCAGCGAATGCGGTTAG

Upstream 100 bases:
NA

Downstream 100 bases:
NA

Product: GGDEF family protein

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:
NA

Sequences:

>Translated_442_residues
MKWIHKLIFALAICTISLVALYSVLGDTRKLVITPERFAYFATDDRAAGGITTTEMAIDGQTIDLSCNMQTANYPWPYCG
ISIFTDTLEATKGIDLSNYHTIRLKLSYDKTGESNGRLRFYLRNFNPAYSTADNEYTYKYNGMEFTPSASMETIEIPLAN
LQVMTWWLADNKIPIEHSAPEYSNVTRIELATGSGAANGEHHIRIERIEFEGAYLAQETLLFALLLSWVLLGLAFSLNEL
RKSKHTLAESRFRQEHLKKMNADLRAQNYEFAELAHRDALTGAMNRHAVRTWLEKKARTVRWGQEQLSVLYLDLDKFKRV
NDRYGHQMGDDILREFVLVVASSLEEGERLVRWGGEEFIVFLPETTLQQAVEKAEMIRKNVSNHLWVHGGMLTCSIGVAQ
MQEERVTEFLARADDALYQAKHRGRNRVEVNYGMQKITSECG
>Mature_442_residues
MKWIHKLIFALAICTISLVALYSVLGDTRKLVITPERFAYFATDDRAAGGITTTEMAIDGQTIDLSCNMQTANYPWPYCG
ISIFTDTLEATKGIDLSNYHTIRLKLSYDKTGESNGRLRFYLRNFNPAYSTADNEYTYKYNGMEFTPSASMETIEIPLAN
LQVMTWWLADNKIPIEHSAPEYSNVTRIELATGSGAANGEHHIRIERIEFEGAYLAQETLLFALLLSWVLLGLAFSLNEL
RKSKHTLAESRFRQEHLKKMNADLRAQNYEFAELAHRDALTGAMNRHAVRTWLEKKARTVRWGQEQLSVLYLDLDKFKRV
NDRYGHQMGDDILREFVLVVASSLEEGERLVRWGGEEFIVFLPETTLQQAVEKAEMIRKNVSNHLWVHGGMLTCSIGVAQ
MQEERVTEFLARADDALYQAKHRGRNRVEVNYGMQKITSECG

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI87081881, Length=155, Percent_Identity=40.6451612903226, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1787262, Length=164, Percent_Identity=35.9756097560976, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI145693134, Length=158, Percent_Identity=37.9746835443038, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1787541, Length=159, Percent_Identity=35.8490566037736, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI87082007, Length=179, Percent_Identity=34.0782122905028, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1787816, Length=154, Percent_Identity=34.4155844155844, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1788381, Length=161, Percent_Identity=33.5403726708075, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI1787802, Length=228, Percent_Identity=29.3859649122807, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1786584, Length=171, Percent_Identity=32.1637426900585, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1787056, Length=169, Percent_Identity=33.7278106508876, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1788085, Length=169, Percent_Identity=32.5443786982249, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87081974, Length=151, Percent_Identity=27.8145695364238, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1788956, Length=165, Percent_Identity=31.5151515151515, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI87081977, Length=106, Percent_Identity=35.8490566037736, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 50505; Mature: 50505

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKWIHKLIFALAICTISLVALYSVLGDTRKLVITPERFAYFATDDRAAGGITTTEMAIDG
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHEEEEECCCCCCCCCEEEEEEECC
QTIDLSCNMQTANYPWPYCGISIFTDTLEATKGIDLSNYHTIRLKLSYDKTGESNGRLRF
EEEEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCCCCEEEE
YLRNFNPAYSTADNEYTYKYNGMEFTPSASMETIEIPLANLQVMTWWLADNKIPIEHSAP
EEECCCCCCCCCCCCEEEEECCEEECCCCCCEEEEEECCCEEEEEEEEECCCCCCCCCCC
EYSNVTRIELATGSGAANGEHHIRIERIEFEGAYLAQETLLFALLLSWVLLGLAFSLNEL
CCCCEEEEEEECCCCCCCCCCEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RKSKHTLAESRFRQEHLKKMNADLRAQNYEFAELAHRDALTGAMNRHAVRTWLEKKARTV
HHHHHHHHHHHHHHHHHHHHCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RWGQEQLSVLYLDLDKFKRVNDRYGHQMGDDILREFVLVVASSLEEGERLVRWGGEEFIV
HCCCHHHEEEEEEHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
FLPETTLQQAVEKAEMIRKNVSNHLWVHGGMLTCSIGVAQMQEERVTEFLARADDALYQA
ECCCHHHHHHHHHHHHHHHCCCCCEEEECCEEEEECCHHHHHHHHHHHHHHHHHHHHHHH
KHRGRNRVEVNYGMQKITSECG
HHCCCCEEEEECCHHHHHHHCC
>Mature Secondary Structure
MKWIHKLIFALAICTISLVALYSVLGDTRKLVITPERFAYFATDDRAAGGITTTEMAIDG
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHEEEEECCCCCCCCCEEEEEEECC
QTIDLSCNMQTANYPWPYCGISIFTDTLEATKGIDLSNYHTIRLKLSYDKTGESNGRLRF
EEEEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCCCCEEEE
YLRNFNPAYSTADNEYTYKYNGMEFTPSASMETIEIPLANLQVMTWWLADNKIPIEHSAP
EEECCCCCCCCCCCCEEEEECCEEECCCCCCEEEEEECCCEEEEEEEEECCCCCCCCCCC
EYSNVTRIELATGSGAANGEHHIRIERIEFEGAYLAQETLLFALLLSWVLLGLAFSLNEL
CCCCEEEEEEECCCCCCCCCCEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RKSKHTLAESRFRQEHLKKMNADLRAQNYEFAELAHRDALTGAMNRHAVRTWLEKKARTV
HHHHHHHHHHHHHHHHHHHHCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RWGQEQLSVLYLDLDKFKRVNDRYGHQMGDDILREFVLVVASSLEEGERLVRWGGEEFIV
HCCCHHHEEEEEEHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
FLPETTLQQAVEKAEMIRKNVSNHLWVHGGMLTCSIGVAQMQEERVTEFLARADDALYQA
ECCCHHHHHHHHHHHHHHHCCCCCEEEECCEEEEECCHHHHHHHHHHHHHHHHHHHHHHH
KHRGRNRVEVNYGMQKITSECG
HHCCCCEEEEECCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA