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The map label for this gene is rlmL [H]

Identifier: 326424050

GI number: 326424050

Start: 2670721

End: 2672844

Strand: Reverse

Name: rlmL [H]

Synonym: NA

Alternate gene names: 326424050

Gene position: 2672844-2670721 (Counterclockwise)

Preceding gene: 27365929

Following gene: 27365926

Centisome position: 81.44

GC content: 45.43

Gene sequence:

>2124_bases
ATGAACCAATATCTTGCGGTTACCTCAAATGGTCTTGAGAACCTTCTTGCTGAAGAACTAACAAAACTTGGAATTGCAGA
CGCTAAGCCAGTACAGGCTGGAGTAAGATTTAAAGCCTCTAATGAACAGGTTTATCGTTGCTGTCTATGGAGTAGGTTGG
CATCGCGTTTTGTTCGCGTGTTGTCCGAGTTTACCTGTCAAAGTGATATGGACTTGTATTTGGCAACGTCCTCTATCAAT
TGGGTAAATCAATTCCACAGCACTAAGAAGTTTGTGGTTGATTTCAACGGCACGAACCGTGAGATTCGTAACAGCCAGTA
CGGTGCGATGAAAGTGAAAGATGGCATCGTAGATTGTTTCGAAAAGAAAAACCTACCACGTCCAAATATCAGTAAAGAGC
GTCCGGATGTACGTATCCATGTTCGTCTCCACAAAGACAAAGCGATTCTTGGTGTTGATATGGTCGGGAATGGCTTGCAC
CAACGTGGCTATCGTCCTGCATCTGGTAAAGCACCACTCCGTGAAACCTTGGCTGCTGCCATCATCCTTCGCTCAGGCTG
GACAGAGGAACAACCGCTTTTGGATCCAATGTGTGGTTCAGGTACGTTGTTGATAGAGGCGGCCATGATGGCAGCAAACA
TGGCGCCTGGCGTAAAACGCGAAAAGTGGGTCTTTGAATCACTTGAAGATTTCGAGCCAGAAGTGTGGGCAGAAATAAAA
TCAGAAGCAAATGTCATTGCTCGAAGAGGTGTCAAAAAAGTTGGCGCAAGATTCTATGGCTTTGATAATGATGAAAAAGT
GCTGAAAATTGCCCAAGATAACGCTCGCAGAGCAGGGGTTGCGGATCTTATCGAGTTTAAGTTAGGTGACGCCGCTACAG
TGTCACGTCCTCAAGAATTTGAGAATGGTGTAATTGTTTGTAACCCACCTTATGGTGAGCGACTTGGCACAGAGCCAGGA
TTGATTGCACTATACACAGCGTTTGGTGGCCAGTTAAAAGCCCAGTTTGGTGGGTGTACGGCATCTATTTTCTCCAGTTC
AGATGAGCTGTTGAGCTGTCTTCGCATGCGTGCGGAAAAACAGTTTAAATTGAATAACGGTGCGTTACCTTGTCACCAAA
AAAACTATACGATCGCACAAAGAAGTGCAGAGCAGATTGAAGGTGCGGATGGCCCGCTTTCTATCGCACCAGACTTTGCT
AATCGCCTAAAGAAAAATATCGGTAAGATTGGCAAATGGGCTCAAAAAGAACAGCTCGATTGTTACCGCATCTATGATGC
CGACCTGCCAGAATACAACGTGGCGATAGACGTCTATCAAGATCACTTGGTGATTCAAGAATACGCCGCGCCGAAAAACA
TTCCAGAAGAGAAGGCAAAGCGTCGCCTGACGGATATCATTCGTGCTGCGATTCAGGTAACTGGCGTAGAAGCCAACAAA
GTTGTGCTGAAGGTTCGTGAGAAACAAAAAGGCCGTAACCAATATCAAAAATTGGGTCAAGTTTCTGAGCGAATGGAAGT
CAACGAGTATGGTGCAAAGCTGATCGTCAATTTGTACGATTATCTGGATACGGGGTTGTTCCTGGATCATAAGATCACGC
GCCGTCGTTTGGGCGAAATGGCAAAAGGAGCCGATTTCCTTAACCTGTTTGCTTATACAGGGAGTGCCACGGTACACGCT
GCTCTTGGTGGTGCCAGCTCGACAACAACGGTTGACATGTCGAACACCTACCTTGAGTGGGCAAAAGACAACATGTCTGC
AAATGGTTGTGTCGGACGTCAACATAAGTTTGAGCAGGCAGATTGTTTACAGTGGTTAGAGAAAGCGCAGGGGCAGTACG
ATTTAATCTTTATCGATCCGCCTACGTTTTCAAACTCAAAGCGCATGGAAGACTCGTTTGACGTTCAGCGCGACCATATC
AAACTGATGAAAAACTTAAAGCGTTTGCTAAGAGAAAACGGCACGATTGTTTTCTCTAATAACAAGCGTCATTTCAAAAT
GGATATGGAGGAAATGGAAGAGTTGGGTTTAGACGCGAAGAACATTTCTTCTCAGACGCTGCCACTCGATTTCTCAAGAA
ACAAACATATTCATAACTGTTGGGTCGTCACCCACAAAAAATAG

Upstream 100 bases:
NA

Downstream 100 bases:
NA

Product: 23S rRNA (guanine-N-2-) -methyltransferase rlmL

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL [H]

Number of amino acids: Translated: 707; Mature: 707

Protein sequence:
NA

Sequences:

>Translated_707_residues
MNQYLAVTSNGLENLLAEELTKLGIADAKPVQAGVRFKASNEQVYRCCLWSRLASRFVRVLSEFTCQSDMDLYLATSSIN
WVNQFHSTKKFVVDFNGTNREIRNSQYGAMKVKDGIVDCFEKKNLPRPNISKERPDVRIHVRLHKDKAILGVDMVGNGLH
QRGYRPASGKAPLRETLAAAIILRSGWTEEQPLLDPMCGSGTLLIEAAMMAANMAPGVKREKWVFESLEDFEPEVWAEIK
SEANVIARRGVKKVGARFYGFDNDEKVLKIAQDNARRAGVADLIEFKLGDAATVSRPQEFENGVIVCNPPYGERLGTEPG
LIALYTAFGGQLKAQFGGCTASIFSSSDELLSCLRMRAEKQFKLNNGALPCHQKNYTIAQRSAEQIEGADGPLSIAPDFA
NRLKKNIGKIGKWAQKEQLDCYRIYDADLPEYNVAIDVYQDHLVIQEYAAPKNIPEEKAKRRLTDIIRAAIQVTGVEANK
VVLKVREKQKGRNQYQKLGQVSERMEVNEYGAKLIVNLYDYLDTGLFLDHKITRRRLGEMAKGADFLNLFAYTGSATVHA
ALGGASSTTTVDMSNTYLEWAKDNMSANGCVGRQHKFEQADCLQWLEKAQGQYDLIFIDPPTFSNSKRMEDSFDVQRDHI
KLMKNLKRLLRENGTIVFSNNKRHFKMDMEEMEELGLDAKNISSQTLPLDFSRNKHIHNCWVVTHKK
>Mature_707_residues
MNQYLAVTSNGLENLLAEELTKLGIADAKPVQAGVRFKASNEQVYRCCLWSRLASRFVRVLSEFTCQSDMDLYLATSSIN
WVNQFHSTKKFVVDFNGTNREIRNSQYGAMKVKDGIVDCFEKKNLPRPNISKERPDVRIHVRLHKDKAILGVDMVGNGLH
QRGYRPASGKAPLRETLAAAIILRSGWTEEQPLLDPMCGSGTLLIEAAMMAANMAPGVKREKWVFESLEDFEPEVWAEIK
SEANVIARRGVKKVGARFYGFDNDEKVLKIAQDNARRAGVADLIEFKLGDAATVSRPQEFENGVIVCNPPYGERLGTEPG
LIALYTAFGGQLKAQFGGCTASIFSSSDELLSCLRMRAEKQFKLNNGALPCHQKNYTIAQRSAEQIEGADGPLSIAPDFA
NRLKKNIGKIGKWAQKEQLDCYRIYDADLPEYNVAIDVYQDHLVIQEYAAPKNIPEEKAKRRLTDIIRAAIQVTGVEANK
VVLKVREKQKGRNQYQKLGQVSERMEVNEYGAKLIVNLYDYLDTGLFLDHKITRRRLGEMAKGADFLNLFAYTGSATVHA
ALGGASSTTTVDMSNTYLEWAKDNMSANGCVGRQHKFEQADCLQWLEKAQGQYDLIFIDPPTFSNSKRMEDSFDVQRDHI
KLMKNLKRLLRENGTIVFSNNKRHFKMDMEEMEELGLDAKNISSQTLPLDFSRNKHIHNCWVVTHKK

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1787181, Length=705, Percent_Identity=56.8794326241135, Blast_Score=836, Evalue=0.0,
Organism=Escherichia coli, GI145693111, Length=264, Percent_Identity=27.2727272727273, Blast_Score=72, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114 [H]

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020 [H]

EC number: =2.1.1.173 [H]

Molecular weight: Translated: 79661; Mature: 79661

Theoretical pI: Translated: 8.64; Mature: 8.64

Prosite motif: PS51165 THUMP ; PS00092 N6_MTASE ; PS01261 UPF0020

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQYLAVTSNGLENLLAEELTKLGIADAKPVQAGVRFKASNEQVYRCCLWSRLASRFVRV
CCCEEEEECCCHHHHHHHHHHHCCCCCCCCHHCCCEEECCCHHHHHHHHHHHHHHHHHHH
LSEFTCQSDMDLYLATSSINWVNQFHSTKKFVVDFNGTNREIRNSQYGAMKVKDGIVDCF
HHHHCCCCCCEEEEEECCCHHHHHHCCCCEEEEEECCCCCHHCCCCCCCEEEHHHHHHHH
EKKNLPRPNISKERPDVRIHVRLHKDKAILGVDMVGNGLHQRGYRPASGKAPLRETLAAA
HHCCCCCCCCCCCCCCEEEEEEEECCCEEEEEHHHCCCHHHCCCCCCCCCCHHHHHHHHH
IILRSGWTEEQPLLDPMCGSGTLLIEAAMMAANMAPGVKREKWVFESLEDFEPEVWAEIK
HHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHH
SEANVIARRGVKKVGARFYGFDNDEKVLKIAQDNARRAGVADLIEFKLGDAATVSRPQEF
HHHHHHHHHHHHHHCHHHCCCCCCHHHEEEHHCCCCCCCHHHHHHHCCCCCCCCCCCHHH
ENGVIVCNPPYGERLGTEPGLIALYTAFGGQLKAQFGGCTASIFSSSDELLSCLRMRAEK
CCCEEEECCCCCCCCCCCCCEEEEEECCCCEEEECCCCEEHHHHCCCHHHHHHHHHHHHH
QFKLNNGALPCHQKNYTIAQRSAEQIEGADGPLSIAPDFANRLKKNIGKIGKWAQKEQLD
CEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCCEECHHHHHHHHHHHHHHCCHHHHCCCC
CYRIYDADLPEYNVAIDVYQDHLVIQEYAAPKNIPEEKAKRRLTDIIRAAIQVTGVEANK
EEEEECCCCCCCEEEEEEECCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCE
VVLKVREKQKGRNQYQKLGQVSERMEVNEYGAKLIVNLYDYLDTGLFLDHKITRRRLGEM
EEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHH
AKGADFLNLFAYTGSATVHAALGGASSTTTVDMSNTYLEWAKDNMSANGCVGRQHKFEQA
HCCCHHHHHEEECCCCEEEEEECCCCCCEEEECCCHHHHHHHCCCCCCCCCCCCCCCCHH
DCLQWLEKAQGQYDLIFIDPPTFSNSKRMEDSFDVQRDHIKLMKNLKRLLRENGTIVFSN
HHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECC
NKRHFKMDMEEMEELGLDAKNISSQTLPLDFSRNKHIHNCWVVTHKK
CCCEEEECHHHHHHCCCCHHCCCCCCCCEECCCCCCEEEEEEEEECC
>Mature Secondary Structure
MNQYLAVTSNGLENLLAEELTKLGIADAKPVQAGVRFKASNEQVYRCCLWSRLASRFVRV
CCCEEEEECCCHHHHHHHHHHHCCCCCCCCHHCCCEEECCCHHHHHHHHHHHHHHHHHHH
LSEFTCQSDMDLYLATSSINWVNQFHSTKKFVVDFNGTNREIRNSQYGAMKVKDGIVDCF
HHHHCCCCCCEEEEEECCCHHHHHHCCCCEEEEEECCCCCHHCCCCCCCEEEHHHHHHHH
EKKNLPRPNISKERPDVRIHVRLHKDKAILGVDMVGNGLHQRGYRPASGKAPLRETLAAA
HHCCCCCCCCCCCCCCEEEEEEEECCCEEEEEHHHCCCHHHCCCCCCCCCCHHHHHHHHH
IILRSGWTEEQPLLDPMCGSGTLLIEAAMMAANMAPGVKREKWVFESLEDFEPEVWAEIK
HHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHH
SEANVIARRGVKKVGARFYGFDNDEKVLKIAQDNARRAGVADLIEFKLGDAATVSRPQEF
HHHHHHHHHHHHHHCHHHCCCCCCHHHEEEHHCCCCCCCHHHHHHHCCCCCCCCCCCHHH
ENGVIVCNPPYGERLGTEPGLIALYTAFGGQLKAQFGGCTASIFSSSDELLSCLRMRAEK
CCCEEEECCCCCCCCCCCCCEEEEEECCCCEEEECCCCEEHHHHCCCHHHHHHHHHHHHH
QFKLNNGALPCHQKNYTIAQRSAEQIEGADGPLSIAPDFANRLKKNIGKIGKWAQKEQLD
CEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCCEECHHHHHHHHHHHHHHCCHHHHCCCC
CYRIYDADLPEYNVAIDVYQDHLVIQEYAAPKNIPEEKAKRRLTDIIRAAIQVTGVEANK
EEEEECCCCCCCEEEEEEECCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCE
VVLKVREKQKGRNQYQKLGQVSERMEVNEYGAKLIVNLYDYLDTGLFLDHKITRRRLGEM
EEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHH
AKGADFLNLFAYTGSATVHAALGGASSTTTVDMSNTYLEWAKDNMSANGCVGRQHKFEQA
HCCCHHHHHEEECCCCEEEEEECCCCCCEEEECCCHHHHHHHCCCCCCCCCCCCCCCCHH
DCLQWLEKAQGQYDLIFIDPPTFSNSKRMEDSFDVQRDHIKLMKNLKRLLRENGTIVFSN
HHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECC
NKRHFKMDMEEMEELGLDAKNISSQTLPLDFSRNKHIHNCWVVTHKK
CCCEEEECHHHHHHCCCCHHCCCCCCCCEECCCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA