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The map label for this gene is slt [H]

Identifier: 326423719

GI number: 326423719

Start: 485980

End: 487935

Strand: Reverse

Name: slt [H]

Synonym: NA

Alternate gene names: 326423719

Gene position: 487935-485980 (Counterclockwise)

Preceding gene: 27363958

Following gene: 27363953

Centisome position: 14.87

GC content: 46.01

Gene sequence:

>1956_bases
ATGACGCGAATGAATTTAAAAAATATTGGGAGAGAGTCGGCCACAAAATGGCTGATGGCAGCGTTGTGTGGCGTGGCGAT
GAATGTCAGTGCTGCGGATGTGGTGACATTAGAAAAACAGCGCGCCTTGTATGAACAAGCGCAAAAACTGTTGGATGACA
AAAACGATGAGGCCTATGTACAAGTGCGTTCTCAACTGGACGACTATGCTCTTACGCCTTATCTCGATTACCGTTTTTTC
CTGGCGCAGCTGTCAAAAAAATCGCCAACAGAAGTGGAGCAATTTATCACTCAAAGCCGTGATTACCCGTTTTCCCAACG
TATTCGCGCGCCATATCTTGATGCTTTATATCGCCAACAAGCATGGTCTGAGGTGGTGTCATTTCAGCAGCAAGAGCCGA
GAGGCGAAAGTTATCAATGTATTTATTACTACGCGCAATACAATCAAGGGCAGAAAAAACTGGCCTATCAAGGCGCAGAG
CAATTATGGCTAAGCGGATTAGAAATTGCGGATGAGTGTGATCATTTGTTTACGGTTTGGGAGCAGGCGGGAGGACGAAC
CGACAACCTGATTTTGCAACGTATGTTGCTTGCGTTTGAGCAGAAAAACAGCAAGTTAATGACTTATTTGCTGAAATTGC
CGAAAAGTAGCCAAGCGAAAAAGCAAGCCCAAGAGATGAAAGCGCTGTTCAATCGTCCTGAGTCGGTGTTGGAGTTTGCC
AAGCAGCATAAAGCCAATGAATTTTATCGTAAGCAAACTGAGCTAGCACTAGAGAAACTGGCACGTAAAGATATCAAACG
TGCTCAAGAGCAGTACGCACAGGTCATCAAAGCTCAAAAGTTTTCATCGGTACAGGCTCAGGAATCGGCCGAATATTTGG
CTTTCCGTTTGATTAATACCGAATCGGAAGCGCTGGCTCAGTGGCGTGATGCAGTGCTGAAAAAATCACATAAGCAAAAC
CTCATTGAGCGCCGAATTCGTCTTGCGTTACAACAGGCGGATTGGAAAGGCGTGCAAAGCTGGGTTGCTCAGTTGGATGA
TGAACATCGAAACTCATTGCGTTGGCAATATTGGTTGGGGCGCAGTGAAATTGAAATGGGCAAAGCAACCCAAGGGAAGC
TGCGACTGAGTAAACTGGTTGGCCAACGTAATTTTTATAGTGTCGCGGCAGCGAAAGAGATCGGGCAGTCTATTAATTAT
CCTACCTCCACATTGAAGTTGGATAGCAGCTTGGTTGAACCTTATCGTCGTTCATTAGCGCGTATCGAAGAATTGATTGC
GCTAGACAAAATTGCCGCGGCAAAAAGCGAGTGGCAATACCTGTTATCTCGCAGTGACGAACAAACTCAAGGCATGCTTG
CGGTCTATGCGGCCTCTAAGCGTTGGTATCATTTGACGGTGACCGCCAGCATTGCGGCGCAAATGTGGGACAACATGGAA
ATTCGTTTTCCGGTGGCGCATCGCTGGTGGTTCAACTTCTATGCTGAGAAGCACGATATTGATCCTATCATGTTGATGTC
ACTTGCCCGTCAAGAGAGCGGTATGGACGCAGAAGCCACCTCTCCTGTGGGGGCTCGCGGTATCATGCAAATTATGCCGT
CCACTGCGAAGTACACAGCAAAGAAGTATCAATTGAGCTATGAAGGTTCTGATGACTTATACCAAGTCGGTAAGAACATC
GAAATTGGCAGCCATTATCTCAATGGTTTATTGGAACAGTACGATAATAATCGAATTTTTGCGTTTGCCGCTTATAATGC
AGGTCCACATCGAGTAAAAAGCTGGCGTGAACGTACTGGCGGGAAATTGGATGCGTACGCGTTCATTGAAGCCATTCCAT
TTAAAGAAACGCGTGGGTATGTACAAAATATCCTGATGTTTGAAACCTATTATCGAGACATTCTCGGGGTAGATGGTGCG
TTCCTAAATCAGCATGAGCTTGAGACTAAATACTGA

Upstream 100 bases:
NA

Downstream 100 bases:
NA

Product: soluble lytic murein transglycosylase

Products: 1,6-Anhydrobond [C]

Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]

Number of amino acids: Translated: 651; Mature: 650

Protein sequence:
NA

Sequences:

>Translated_651_residues
MTRMNLKNIGRESATKWLMAALCGVAMNVSAADVVTLEKQRALYEQAQKLLDDKNDEAYVQVRSQLDDYALTPYLDYRFF
LAQLSKKSPTEVEQFITQSRDYPFSQRIRAPYLDALYRQQAWSEVVSFQQQEPRGESYQCIYYYAQYNQGQKKLAYQGAE
QLWLSGLEIADECDHLFTVWEQAGGRTDNLILQRMLLAFEQKNSKLMTYLLKLPKSSQAKKQAQEMKALFNRPESVLEFA
KQHKANEFYRKQTELALEKLARKDIKRAQEQYAQVIKAQKFSSVQAQESAEYLAFRLINTESEALAQWRDAVLKKSHKQN
LIERRIRLALQQADWKGVQSWVAQLDDEHRNSLRWQYWLGRSEIEMGKATQGKLRLSKLVGQRNFYSVAAAKEIGQSINY
PTSTLKLDSSLVEPYRRSLARIEELIALDKIAAAKSEWQYLLSRSDEQTQGMLAVYAASKRWYHLTVTASIAAQMWDNME
IRFPVAHRWWFNFYAEKHDIDPIMLMSLARQESGMDAEATSPVGARGIMQIMPSTAKYTAKKYQLSYEGSDDLYQVGKNI
EIGSHYLNGLLEQYDNNRIFAFAAYNAGPHRVKSWRERTGGKLDAYAFIEAIPFKETRGYVQNILMFETYYRDILGVDGA
FLNQHELETKY
>Mature_650_residues
TRMNLKNIGRESATKWLMAALCGVAMNVSAADVVTLEKQRALYEQAQKLLDDKNDEAYVQVRSQLDDYALTPYLDYRFFL
AQLSKKSPTEVEQFITQSRDYPFSQRIRAPYLDALYRQQAWSEVVSFQQQEPRGESYQCIYYYAQYNQGQKKLAYQGAEQ
LWLSGLEIADECDHLFTVWEQAGGRTDNLILQRMLLAFEQKNSKLMTYLLKLPKSSQAKKQAQEMKALFNRPESVLEFAK
QHKANEFYRKQTELALEKLARKDIKRAQEQYAQVIKAQKFSSVQAQESAEYLAFRLINTESEALAQWRDAVLKKSHKQNL
IERRIRLALQQADWKGVQSWVAQLDDEHRNSLRWQYWLGRSEIEMGKATQGKLRLSKLVGQRNFYSVAAAKEIGQSINYP
TSTLKLDSSLVEPYRRSLARIEELIALDKIAAAKSEWQYLLSRSDEQTQGMLAVYAASKRWYHLTVTASIAAQMWDNMEI
RFPVAHRWWFNFYAEKHDIDPIMLMSLARQESGMDAEATSPVGARGIMQIMPSTAKYTAKKYQLSYEGSDDLYQVGKNIE
IGSHYLNGLLEQYDNNRIFAFAAYNAGPHRVKSWRERTGGKLDAYAFIEAIPFKETRGYVQNILMFETYYRDILGVDGAF
LNQHELETKY

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=640, Percent_Identity=35.625, Blast_Score=410, Evalue=1e-115,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016026
- InterPro:   IPR008258
- InterPro:   IPR012289
- InterPro:   IPR008939
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 75509; Mature: 75377

Theoretical pI: Translated: 9.15; Mature: 9.15

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRMNLKNIGRESATKWLMAALCGVAMNVSAADVVTLEKQRALYEQAQKLLDDKNDEAYV
CCCCCHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
QVRSQLDDYALTPYLDYRFFLAQLSKKSPTEVEQFITQSRDYPFSQRIRAPYLDALYRQQ
HHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHH
AWSEVVSFQQQEPRGESYQCIYYYAQYNQGQKKLAYQGAEQLWLSGLEIADECDHLFTVW
HHHHHHHHHHCCCCCCCEEEEEEEEECCCCHHHHHHHCHHHHHHHCCHHHHHHHHHHHHH
EQAGGRTDNLILQRMLLAFEQKNSKLMTYLLKLPKSSQAKKQAQEMKALFNRPESVLEFA
HHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHH
KQHKANEFYRKQTELALEKLARKDIKRAQEQYAQVIKAQKFSSVQAQESAEYLAFRLINT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECC
ESEALAQWRDAVLKKSHKQNLIERRIRLALQQADWKGVQSWVAQLDDEHRNSLRWQYWLG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCHHHHHCCEEEEECC
RSEIEMGKATQGKLRLSKLVGQRNFYSVAAAKEIGQSINYPTSTLKLDSSLVEPYRRSLA
CCHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHEEHHHHHHHHHHHHHH
RIEELIALDKIAAAKSEWQYLLSRSDEQTQGMLAVYAASKRWYHLTVTASIAAQMWDNME
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCEEEEEECCCCEEEEEEHHHHHHHHHCCCE
IRFPVAHRWWFNFYAEKHDIDPIMLMSLARQESGMDAEATSPVGARGIMQIMPSTAKYTA
EEECHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCHHHHHH
KKYQLSYEGSDDLYQVGKNIEIGSHYLNGLLEQYDNNRIFAFAAYNAGPHRVKSWRERTG
HHEEEECCCCHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHCC
GKLDAYAFIEAIPFKETRGYVQNILMFETYYRDILGVDGAFLNQHELETKY
CCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TRMNLKNIGRESATKWLMAALCGVAMNVSAADVVTLEKQRALYEQAQKLLDDKNDEAYV
CCCCHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
QVRSQLDDYALTPYLDYRFFLAQLSKKSPTEVEQFITQSRDYPFSQRIRAPYLDALYRQQ
HHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHH
AWSEVVSFQQQEPRGESYQCIYYYAQYNQGQKKLAYQGAEQLWLSGLEIADECDHLFTVW
HHHHHHHHHHCCCCCCCEEEEEEEEECCCCHHHHHHHCHHHHHHHCCHHHHHHHHHHHHH
EQAGGRTDNLILQRMLLAFEQKNSKLMTYLLKLPKSSQAKKQAQEMKALFNRPESVLEFA
HHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHH
KQHKANEFYRKQTELALEKLARKDIKRAQEQYAQVIKAQKFSSVQAQESAEYLAFRLINT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECC
ESEALAQWRDAVLKKSHKQNLIERRIRLALQQADWKGVQSWVAQLDDEHRNSLRWQYWLG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCHHHHHCCEEEEECC
RSEIEMGKATQGKLRLSKLVGQRNFYSVAAAKEIGQSINYPTSTLKLDSSLVEPYRRSLA
CCHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHEEHHHHHHHHHHHHHH
RIEELIALDKIAAAKSEWQYLLSRSDEQTQGMLAVYAASKRWYHLTVTASIAAQMWDNME
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCEEEEEECCCCEEEEEEHHHHHHHHHCCCE
IRFPVAHRWWFNFYAEKHDIDPIMLMSLARQESGMDAEATSPVGARGIMQIMPSTAKYTA
EEECHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCHHHHHH
KKYQLSYEGSDDLYQVGKNIEIGSHYLNGLLEQYDNNRIFAFAAYNAGPHRVKSWRERTG
HHEEEECCCCHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHCC
GKLDAYAFIEAIPFKETRGYVQNILMFETYYRDILGVDGAFLNQHELETKY
CCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]