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The map label for this gene is mepA [H]

Identifier: 326423718

GI number: 326423718

Start: 470860

End: 471690

Strand: Reverse

Name: mepA [H]

Synonym: NA

Alternate gene names: 326423718

Gene position: 471690-470860 (Counterclockwise)

Preceding gene: 27363946

Following gene: 27363935

Centisome position: 14.37

GC content: 47.65

Gene sequence:

>831_bases
ATGTTCGCTAAAATACTGGCGTCATTGTGCGCGATGTATTCAATACCTACTTGGGCATTACAAATGACAGCGTGGGAACT
GGTGACCGCACCAACGCGTGAACGTGCTCAATCAATAGGATCGTACGCAAATGGCTGTTTAGATGGTGGTATCGCTTTAC
CACTAGAGGGGGAAGGGTATCAAGTTGTGCGTGCAGAGCGTAATCGCTACTTTGCGCATCCAGAAACCGTCACTTTTATA
CAAGAGTTAGGCTATTACGCGGATACACATTTGGCTTCGACTCTAATGATTGCGGATGTCAGTTTACCACGAGGTGGGCG
TTTTTCTTTTGGTCATGCGAGTCATCAAACCGGACTCGATATTGATGTATGGCTTAAACTAAACACCTCTCCTTTGAATA
AATATGAGCTTGAACAGGCAAAGACTGCGAGTGTCGTCAATTTCGATACCATGACTATTCGTGCCGATGAATGGCGTGAT
GAGCAATTCGAGTTGATCCGAGCCGCAGCCGAAAATGGCAGGGTAGCGAGAATTTTTGTCCACGCCGTTATCAAGCAACG
TTTATGTCAAATGGAAACATCCACTCATAGGTCGTGGCTGAGAAAGGTAAGGCCTTGGTGGGGACATTCTTCACACATGC
ATGTGAGACTCTCTTGCCCACAAGGAAGCGATGACTGTATCGAGCAAGCTTTGCCGCCGGAGGGAGATGGTTGTGGTGAA
GAAGTGTTGAGCTGGATAGAGCGTCCAACGCCACCGGCGAAAAAAGCACTGGAGCCCGAGCCTATCTCTGAGGCATGTGT
GACGGTACTAAATCAGAAACATGAGAAATAA

Upstream 100 bases:
NA

Downstream 100 bases:
NA

Product: Murein endopeptidase

Products: Split Peptidoglycan Strands [C]

Alternate protein names: D-alanyl-D-alanine-endopeptidase; DD-endopeptidase [H]

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:
NA

Sequences:

>Translated_276_residues
MFAKILASLCAMYSIPTWALQMTAWELVTAPTRERAQSIGSYANGCLDGGIALPLEGEGYQVVRAERNRYFAHPETVTFI
QELGYYADTHLASTLMIADVSLPRGGRFSFGHASHQTGLDIDVWLKLNTSPLNKYELEQAKTASVVNFDTMTIRADEWRD
EQFELIRAAAENGRVARIFVHAVIKQRLCQMETSTHRSWLRKVRPWWGHSSHMHVRLSCPQGSDDCIEQALPPEGDGCGE
EVLSWIERPTPPAKKALEPEPISEACVTVLNQKHEK
>Mature_276_residues
MFAKILASLCAMYSIPTWALQMTAWELVTAPTRERAQSIGSYANGCLDGGIALPLEGEGYQVVRAERNRYFAHPETVTFI
QELGYYADTHLASTLMIADVSLPRGGRFSFGHASHQTGLDIDVWLKLNTSPLNKYELEQAKTASVVNFDTMTIRADEWRD
EQFELIRAAAENGRVARIFVHAVIKQRLCQMETSTHRSWLRKVRPWWGHSSHMHVRLSCPQGSDDCIEQALPPEGDGCGE
EVLSWIERPTPPAKKALEPEPISEACVTVLNQKHEK

Specific function: Involved in the removal of murein from the sacculus. May also facilitate integration of nascent murein strands into the sacculus by cleaving the peptide bonds between neighboring strands in mature murein [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M74 family [H]

Homologues:

Organism=Escherichia coli, GI1788668, Length=254, Percent_Identity=45.2755905511811, Blast_Score=239, Evalue=2e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009045
- InterPro:   IPR005073 [H]

Pfam domain/function: PF03411 Peptidase_M74 [H]

EC number: 3.4.99.- [C]

Molecular weight: Translated: 31023; Mature: 31023

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS00477 ALPHA_2_MACROGLOBULIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFAKILASLCAMYSIPTWALQMTAWELVTAPTRERAQSIGSYANGCLDGGIALPLEGEGY
CHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCE
QVVRAERNRYFAHPETVTFIQELGYYADTHLASTLMIADVSLPRGGRFSFGHASHQTGLD
EEEEECCCCEECCCHHHHHHHHHCCHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCCCC
IDVWLKLNTSPLNKYELEQAKTASVVNFDTMTIRADEWRDEQFELIRAAAENGRVARIFV
EEEEEEECCCCCCHHHHHHHHHHEEEEEEEEEEECCCCCHHHHHHHHHHHCCCCCHHHHH
HAVIKQRLCQMETSTHRSWLRKVRPWWGHSSHMHVRLSCPQGSDDCIEQALPPEGDGCGE
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHHCCCCCCCCCHH
EVLSWIERPTPPAKKALEPEPISEACVTVLNQKHEK
HHHHHHHCCCCCHHHCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MFAKILASLCAMYSIPTWALQMTAWELVTAPTRERAQSIGSYANGCLDGGIALPLEGEGY
CHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCE
QVVRAERNRYFAHPETVTFIQELGYYADTHLASTLMIADVSLPRGGRFSFGHASHQTGLD
EEEEECCCCEECCCHHHHHHHHHCCHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCCCC
IDVWLKLNTSPLNKYELEQAKTASVVNFDTMTIRADEWRDEQFELIRAAAENGRVARIFV
EEEEEEECCCCCCHHHHHHHHHHEEEEEEEEEEECCCCCHHHHHHHHHHHCCCCCHHHHH
HAVIKQRLCQMETSTHRSWLRKVRPWWGHSSHMHVRLSCPQGSDDCIEQALPPEGDGCGE
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHHCCCCCCCCCHH
EVLSWIERPTPPAKKALEPEPISEACVTVLNQKHEK
HHHHHHHCCCCCHHHCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: The D-Alanyl-Gamma-Meso-2,6-Diamino- Pimelyl Peptide Bond [C]

Specific reaction: Split The D-Alanyl-Gamma-Meso-2,6-Diamino- Pimelyl Peptide Bond Connecting Neighboring Peptidoglycan Strands. [C]

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Endopeptidases of unknown catalytic mechanism [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA