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The map label for this gene is thiG

Identifier: 32267350

GI number: 32267350

Start: 1773865

End: 1774656

Strand: Direct

Name: thiG

Synonym: HH1851

Alternate gene names: 32267350

Gene position: 1773865-1774656 (Clockwise)

Preceding gene: 32267349

Following gene: 32267351

Centisome position: 98.59

GC content: 41.92

Gene sequence:

>792_bases
ATGAATCATATTAAAGATACTCTCAAAATCGGCTCACATACTTTCGCATCGCGCTTAATTGTCGGGAGCGGAAAATATAA
AGACTTTGCTACCACCAAAGAAGCGACTTTAGCAAGTGGCGCAGAAATTATCACCGTTGCAGTTAGGCGTGTGAATATTA
TGGATAATAAAAGTGAGAATCTTCTTGAAACCTTTAAAGATACAAATATTCAGTTTTTGCCCAACTCTGCTGGGTGTGTC
AATGCTAAGGAAGCAATTACACTCTTTCGTCTTGTGAGAGAAGCAACAGGTATTGATTTTATCAAGCTTGAAATCATAGG
CGATATAGACAAAACACTCTATCCTGATGTAATAGAGAGCCTACAAGCCACAGAGATTCTAGCAAATGAGGGCTTTTGCG
TATTGGCTTATACAAATGATGACCCTATTATGGCAAAACGACTTGAAAATGCGGGAGCAAGCGCGATTATGCCTCTTGCT
GCGCCTATTGGAAGCGGACTTGGAATCCAAAATCGTTATAATATCGGCTTTATCAAAGAAGCAGTAAAAGTGCCTGTGAT
TGTTGATGCGGGGGTAGGCTGTGCGAGTGATGCAAGTATCGCTATGGAGTTAGGAGCAGATGCTGTGCTTACAAATACGG
CAATTGCTCAAGCACAAAATCCTGTAACGATGGCAGAAGCGATGAAATACGCTGTAAAAGCCGGGCGATTAAGCTATCTT
GCTGGACGCATACCACGTAAAGCCTATGCAAGTGCAAGTTCTCCACTTGAAGGTATGATGCAATTTGGTTAG

Upstream 100 bases:

>100_bases
CAGCAAGAGAAGCTACAAAACATATTGCAAATTACGCATTGACACCAAGTATGCTTCTTGAAAGCATTACTACCTTGCAA
TCTCATAAAAGGATACATAA

Downstream 100 bases:

>100_bases
ATTCTAGGCAAAATAATGAAAGATAGAATCTTTAAACAAGATATTGGCAAACAATTTGAATTTGATGCGCAAGTAGCAAG
TGTGTTTGATGATATGCTTG

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MNHIKDTLKIGSHTFASRLIVGSGKYKDFATTKEATLASGAEIITVAVRRVNIMDNKSENLLETFKDTNIQFLPNSAGCV
NAKEAITLFRLVREATGIDFIKLEIIGDIDKTLYPDVIESLQATEILANEGFCVLAYTNDDPIMAKRLENAGASAIMPLA
APIGSGLGIQNRYNIGFIKEAVKVPVIVDAGVGCASDASIAMELGADAVLTNTAIAQAQNPVTMAEAMKYAVKAGRLSYL
AGRIPRKAYASASSPLEGMMQFG

Sequences:

>Translated_263_residues
MNHIKDTLKIGSHTFASRLIVGSGKYKDFATTKEATLASGAEIITVAVRRVNIMDNKSENLLETFKDTNIQFLPNSAGCV
NAKEAITLFRLVREATGIDFIKLEIIGDIDKTLYPDVIESLQATEILANEGFCVLAYTNDDPIMAKRLENAGASAIMPLA
APIGSGLGIQNRYNIGFIKEAVKVPVIVDAGVGCASDASIAMELGADAVLTNTAIAQAQNPVTMAEAMKYAVKAGRLSYL
AGRIPRKAYASASSPLEGMMQFG
>Mature_263_residues
MNHIKDTLKIGSHTFASRLIVGSGKYKDFATTKEATLASGAEIITVAVRRVNIMDNKSENLLETFKDTNIQFLPNSAGCV
NAKEAITLFRLVREATGIDFIKLEIIGDIDKTLYPDVIESLQATEILANEGFCVLAYTNDDPIMAKRLENAGASAIMPLA
APIGSGLGIQNRYNIGFIKEAVKVPVIVDAGVGCASDASIAMELGADAVLTNTAIAQAQNPVTMAEAMKYAVKAGRLSYL
AGRIPRKAYASASSPLEGMMQFG

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family

Homologues:

Organism=Escherichia coli, GI48994993, Length=254, Percent_Identity=44.4881889763779, Blast_Score=213, Evalue=7e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIG_HELHP (Q7VF28)

Other databases:

- EMBL:   AE017125
- RefSeq:   NP_861382.1
- ProteinModelPortal:   Q7VF28
- SMR:   Q7VF28
- GeneID:   1492398
- GenomeReviews:   AE017125_GR
- KEGG:   hhe:HH1851
- NMPDR:   fig|235279.1.peg.1851
- HOGENOM:   HBG296821
- OMA:   VAIRRTN
- ProtClustDB:   PRK00208
- BioCyc:   HHEP235279:HH_1851-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00443
- InterPro:   IPR013785
- InterPro:   IPR008867
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF05690 ThiG; SSF110399 ThiG

EC number: NA

Molecular weight: Translated: 28022; Mature: 28022

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: NA

Important sites: ACT_SITE 102-102 BINDING 164-164

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHIKDTLKIGSHTFASRLIVGSGKYKDFATTKEATLASGAEIITVAVRRVNIMDNKSEN
CCCHHHHHHHCCHHHHHEEEECCCCCCCCCCCCHHHHHCCCCEEEHHHHHHHCCCCCCHH
LLETFKDTNIQFLPNSAGCVNAKEAITLFRLVREATGIDFIKLEIIGDIDKTLYPDVIES
HHHHHHCCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHH
LQATEILANEGFCVLAYTNDDPIMAKRLENAGASAIMPLAAPIGSGLGIQNRYNIGFIKE
HHHHHHHHCCCEEEEEEECCCCHHHHHHHCCCCCEEHHHHHHCCCCCCCCCCCCCHHHHH
AVKVPVIVDAGVGCASDASIAMELGADAVLTNTAIAQAQNPVTMAEAMKYAVKAGRLSYL
HHCCCEEEECCCCCCCCCHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
AGRIPRKAYASASSPLEGMMQFG
HHCCCHHHHHCCCCCHHHHHHCC
>Mature Secondary Structure
MNHIKDTLKIGSHTFASRLIVGSGKYKDFATTKEATLASGAEIITVAVRRVNIMDNKSEN
CCCHHHHHHHCCHHHHHEEEECCCCCCCCCCCCHHHHHCCCCEEEHHHHHHHCCCCCCHH
LLETFKDTNIQFLPNSAGCVNAKEAITLFRLVREATGIDFIKLEIIGDIDKTLYPDVIES
HHHHHHCCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHH
LQATEILANEGFCVLAYTNDDPIMAKRLENAGASAIMPLAAPIGSGLGIQNRYNIGFIKE
HHHHHHHHCCCEEEEEEECCCCHHHHHHHCCCCCEEHHHHHHCCCCCCCCCCCCCHHHHH
AVKVPVIVDAGVGCASDASIAMELGADAVLTNTAIAQAQNPVTMAEAMKYAVKAGRLSYL
HHCCCEEEECCCCCCCCCHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
AGRIPRKAYASASSPLEGMMQFG
HHCCCHHHHHCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12810954