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The map label for this gene is folE

Identifier: 32267211

GI number: 32267211

Start: 1638709

End: 1639329

Strand: Direct

Name: folE

Synonym: HH1712

Alternate gene names: 32267211

Gene position: 1638709-1639329 (Clockwise)

Preceding gene: 32267210

Following gene: 32267212

Centisome position: 91.08

GC content: 38.49

Gene sequence:

>621_bases
TTGCACAATGCAAAACGCGCAATTTTTAAAAAGCGAGAAAATATGCAAAAATCTAGCCACGATTCTATCTTAGAGTGCAA
TGCGCAAGCATTTTTTGAATCTTTATGTCAAAATGTCGGCGAAAATCCTACGCGTGAGGGATTACTTCATACACCAAAGC
GCATTGCTAGTGCATTTTCCGATATGCTAAATGGCTATGCCAAAAGCCCGAAACAAGCGCTTGGTAGTGTCTTTGAAGAT
GGCGTATGTGATGAAATGATAGTGCTTAAAAAGCTACATTTTTACTCTATATGCGAGCATCATCTTTTGCCATTTTTTGG
GCATATTTCTATTGGATATATCCCTGATAAAAAGCTTGTAGGTATTAGTGGCTTAGCAAGATTGACAGAGGTTTTTACAC
ATAGATTACAGATTCAAGAGCGTTTGACTGCCCAGATTGCAGATGCGCTGATTGCTGAGCTTAAACCCAAAGGGGCAATG
ATAGTTTGCCAAGCACGACATCTTTGCCTTGAAATGCGCTCTAATGTGCCACAATCTCATATTATTACTTCTGCTTTACG
CGGACTTTTTAAAAAAGATTCTAGAACAAGAGCAGAATTTATGCAGATTCTCAAAGATTAA

Upstream 100 bases:

>100_bases
AGCATTACTTGGACAACATTTTTAAGCAATGGATAAAATCGCACGAATAAGGTGAGATATAAACATTGAAATTAAAGCAT
AAGCAAAAATATTATACAAT

Downstream 100 bases:

>100_bases
AACCCTCATAAGAGTATTTTTCAAGCTAAATTTTACTTTTTTATTGACAAAGTGCTTTTTTTTTGCAAAAATGCCCCATC
CGTTATCGGATTATTAACTT

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I

Number of amino acids: Translated: 206; Mature: 206

Protein sequence:

>206_residues
MHNAKRAIFKKRENMQKSSHDSILECNAQAFFESLCQNVGENPTREGLLHTPKRIASAFSDMLNGYAKSPKQALGSVFED
GVCDEMIVLKKLHFYSICEHHLLPFFGHISIGYIPDKKLVGISGLARLTEVFTHRLQIQERLTAQIADALIAELKPKGAM
IVCQARHLCLEMRSNVPQSHIITSALRGLFKKDSRTRAEFMQILKD

Sequences:

>Translated_206_residues
MHNAKRAIFKKRENMQKSSHDSILECNAQAFFESLCQNVGENPTREGLLHTPKRIASAFSDMLNGYAKSPKQALGSVFED
GVCDEMIVLKKLHFYSICEHHLLPFFGHISIGYIPDKKLVGISGLARLTEVFTHRLQIQERLTAQIADALIAELKPKGAM
IVCQARHLCLEMRSNVPQSHIITSALRGLFKKDSRTRAEFMQILKD
>Mature_206_residues
MHNAKRAIFKKRENMQKSSHDSILECNAQAFFESLCQNVGENPTREGLLHTPKRIASAFSDMLNGYAKSPKQALGSVFED
GVCDEMIVLKKLHFYSICEHHLLPFFGHISIGYIPDKKLVGISGLARLTEVFTHRLQIQERLTAQIADALIAELKPKGAM
IVCQARHLCLEMRSNVPQSHIITSALRGLFKKDSRTRAEFMQILKD

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family

Homologues:

Organism=Homo sapiens, GI66932968, Length=191, Percent_Identity=41.3612565445026, Blast_Score=167, Evalue=5e-42,
Organism=Homo sapiens, GI4503949, Length=191, Percent_Identity=41.3612565445026, Blast_Score=167, Evalue=5e-42,
Organism=Homo sapiens, GI66932972, Length=150, Percent_Identity=43.3333333333333, Blast_Score=137, Evalue=6e-33,
Organism=Homo sapiens, GI66932970, Length=150, Percent_Identity=43.3333333333333, Blast_Score=137, Evalue=7e-33,
Organism=Escherichia coli, GI1788476, Length=165, Percent_Identity=34.5454545454545, Blast_Score=90, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17560486, Length=180, Percent_Identity=42.7777777777778, Blast_Score=166, Evalue=7e-42,
Organism=Saccharomyces cerevisiae, GI6321706, Length=176, Percent_Identity=43.75, Blast_Score=145, Evalue=4e-36,
Organism=Drosophila melanogaster, GI24656782, Length=175, Percent_Identity=43.4285714285714, Blast_Score=152, Evalue=1e-37,
Organism=Drosophila melanogaster, GI24656796, Length=175, Percent_Identity=43.4285714285714, Blast_Score=152, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24656791, Length=175, Percent_Identity=43.4285714285714, Blast_Score=152, Evalue=2e-37,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): GCH1_HELHP (Q7VFG4)

Other databases:

- EMBL:   AE017125
- RefSeq:   NP_861243.1
- ProteinModelPortal:   Q7VFG4
- SMR:   Q7VFG4
- GeneID:   1492259
- GenomeReviews:   AE017125_GR
- KEGG:   hhe:HH1712
- NMPDR:   fig|235279.1.peg.1712
- HOGENOM:   HBG370191
- OMA:   YALEPRE
- ProtClustDB:   PRK09347
- BioCyc:   HHEP235279:HH_1712-MONOMER
- BRENDA:   3.5.4.16
- GO:   GO:0005737
- HAMAP:   MF_00223
- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234
- TIGRFAMs:   TIGR00063

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =3.5.4.16

Molecular weight: Translated: 23262; Mature: 23262

Theoretical pI: Translated: 9.32; Mature: 9.32

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1; PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHNAKRAIFKKRENMQKSSHDSILECNAQAFFESLCQNVGENPTREGLLHTPKRIASAFS
CCCHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
DMLNGYAKSPKQALGSVFEDGVCDEMIVLKKLHFYSICEHHLLPFFGHISIGYIPDKKLV
HHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCHHH
GISGLARLTEVFTHRLQIQERLTAQIADALIAELKPKGAMIVCQARHLCLEMRSNVPQSH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHCCCHHH
IITSALRGLFKKDSRTRAEFMQILKD
HHHHHHHHHHHCCCHHHHHHHHHHCC
>Mature Secondary Structure
MHNAKRAIFKKRENMQKSSHDSILECNAQAFFESLCQNVGENPTREGLLHTPKRIASAFS
CCCHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
DMLNGYAKSPKQALGSVFEDGVCDEMIVLKKLHFYSICEHHLLPFFGHISIGYIPDKKLV
HHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCHHH
GISGLARLTEVFTHRLQIQERLTAQIADALIAELKPKGAMIVCQARHLCLEMRSNVPQSH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHCCCHHH
IITSALRGLFKKDSRTRAEFMQILKD
HHHHHHHHHHHCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12810954