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The map label for this gene is pcnB

Identifier: 32267207

GI number: 32267207

Start: 1634237

End: 1635643

Strand: Direct

Name: pcnB

Synonym: HH1708

Alternate gene names: 32267207

Gene position: 1634237-1635643 (Clockwise)

Preceding gene: 32267206

Following gene: 32267209

Centisome position: 90.83

GC content: 37.53

Gene sequence:

>1407_bases
ATGTGCGTTATCCTTTTATGTTTATTCCATTGTTTAGACGTTTTATTTTACGATTTGGCATATTGCGTGTGCGATGTGGG
CTTAAGAATAAGCATATTGTCTAAGATGAAAACACATATTTTTACGCCTACTCTCCCAAAAGAGGTTTTATATATCCTTG
AAAGTTTGCAGAGTGCAGGATATGAGGCTTATATTGTGGGCGGTTGTGTGCGAGATATGCTTCTTGCTCAAAACTCCCAT
ATACCTTATGTTATAAATGATTATGATATTACGACTTCCGCCCTGCCACAAGAAGTGATGAAGCTTTTTGCACACACAAT
TCACACAGGGTTAAAATATGGCACTATAAGCGTTTTGATAGAATCTCAAAGTTATGAGGTTACAACCTTTCGTATTGATG
GCTCATATATAAATGCGCGTTCCCCCAAAAATGTTACTTTCACGCGCTCCCTTGCACAGGATTTACAAAGAAGAGATTTT
ACAATTAATGCTCTTGCTTATGCGCCTTGTAGTGGGCTTATTGATGAGGTGGGAGGTTTAGCAGATTTGCAACACAAGCG
TATTGTGTGTGTAGGTGATGCGTTTGCTCGTTTGAATGAAGATATTTTGAGGATTCTACGCGCTTTGCGCTTTAGTTCTA
CACTTGGTTTTGAGATAGATTTTCATACAAAAGAAGCGATTTTTTCACTTTCCTCTAAAGTGTGTCTTATCGCCAAAGAG
CGTATAAGAGTGGAATTTACAAAGCTTTTGTGTGGAGCGTGGGTAAAGGTAGTGCTTGATGAGTTTAGATTTGTGATAAG
TGAAATTCTCTCACAAATGCGTGAAATAATTGCAAATAATACTTTATGGCAACATACACTTTCTATACTAGACAATCTTA
CTCCTCGTAGCCACACTCTAGCGTGGGTAGCGTTTTTGTATCACACAGATGAATTAAAAGCACGAGATATTTTGCAATCG
CTTAAATTTGACACATATAACAAGGACTATATTTGCTCCCTGCTTGTCTATGTTAAAAAGCCTTTAAACAATAATCTCAC
CACGCTTAAGACGATGCTTGTAGATATGGGAGGGCGCAAAAAAGGTGAAGCAATGTTTAAAGATATGCTTAAACTCAAGG
TTGCGGTAATAAATGCGCAAGATACAGCGGATAAAGCGCAAAAACTTCAAATGCTAGATTCTATCCATTCTCTTTTAGAG
TGTGTTCTTGCTTCCTCTGTGCCTTTAGAATTAAAAGAATTATGTCTTAATGGCAGAGATTTAATCTATATCGGAGTGCC
AAAGGGTAAGAGAATAGGGGAGATTCTCCATTTTTTACATCAAGAAGTGTTGCACGAGCGAGTGAGCAATACAAAAGAAG
CATTATTATCAAAAGCACAAGCGTTTTTACAAACCAAAAAAGAGTGA

Upstream 100 bases:

>100_bases
CTCTCCCGCAACAAAGATATAGCATTGTGGTATAAGTTGCTTCATCGCACTTATACAAAACGCACAAGATTCCTTGTATG
CAAAACACTTTTGAGGCATT

Downstream 100 bases:

>100_bases
GATTTAATACATACATTTAAGATTCTTTATAGAATCTCCCCACCAATAAGCTTATATCGCTCCCAATAATGCTCTGAAAA
AGCTTTTACTTGCGCATTTG

Product: poly(A) polymerase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MCVILLCLFHCLDVLFYDLAYCVCDVGLRISILSKMKTHIFTPTLPKEVLYILESLQSAGYEAYIVGGCVRDMLLAQNSH
IPYVINDYDITTSALPQEVMKLFAHTIHTGLKYGTISVLIESQSYEVTTFRIDGSYINARSPKNVTFTRSLAQDLQRRDF
TINALAYAPCSGLIDEVGGLADLQHKRIVCVGDAFARLNEDILRILRALRFSSTLGFEIDFHTKEAIFSLSSKVCLIAKE
RIRVEFTKLLCGAWVKVVLDEFRFVISEILSQMREIIANNTLWQHTLSILDNLTPRSHTLAWVAFLYHTDELKARDILQS
LKFDTYNKDYICSLLVYVKKPLNNNLTTLKTMLVDMGGRKKGEAMFKDMLKLKVAVINAQDTADKAQKLQMLDSIHSLLE
CVLASSVPLELKELCLNGRDLIYIGVPKGKRIGEILHFLHQEVLHERVSNTKEALLSKAQAFLQTKKE

Sequences:

>Translated_468_residues
MCVILLCLFHCLDVLFYDLAYCVCDVGLRISILSKMKTHIFTPTLPKEVLYILESLQSAGYEAYIVGGCVRDMLLAQNSH
IPYVINDYDITTSALPQEVMKLFAHTIHTGLKYGTISVLIESQSYEVTTFRIDGSYINARSPKNVTFTRSLAQDLQRRDF
TINALAYAPCSGLIDEVGGLADLQHKRIVCVGDAFARLNEDILRILRALRFSSTLGFEIDFHTKEAIFSLSSKVCLIAKE
RIRVEFTKLLCGAWVKVVLDEFRFVISEILSQMREIIANNTLWQHTLSILDNLTPRSHTLAWVAFLYHTDELKARDILQS
LKFDTYNKDYICSLLVYVKKPLNNNLTTLKTMLVDMGGRKKGEAMFKDMLKLKVAVINAQDTADKAQKLQMLDSIHSLLE
CVLASSVPLELKELCLNGRDLIYIGVPKGKRIGEILHFLHQEVLHERVSNTKEALLSKAQAFLQTKKE
>Mature_468_residues
MCVILLCLFHCLDVLFYDLAYCVCDVGLRISILSKMKTHIFTPTLPKEVLYILESLQSAGYEAYIVGGCVRDMLLAQNSH
IPYVINDYDITTSALPQEVMKLFAHTIHTGLKYGTISVLIESQSYEVTTFRIDGSYINARSPKNVTFTRSLAQDLQRRDF
TINALAYAPCSGLIDEVGGLADLQHKRIVCVGDAFARLNEDILRILRALRFSSTLGFEIDFHTKEAIFSLSSKVCLIAKE
RIRVEFTKLLCGAWVKVVLDEFRFVISEILSQMREIIANNTLWQHTLSILDNLTPRSHTLAWVAFLYHTDELKARDILQS
LKFDTYNKDYICSLLVYVKKPLNNNLTTLKTMLVDMGGRKKGEAMFKDMLKLKVAVINAQDTADKAQKLQMLDSIHSLLE
CVLASSVPLELKELCLNGRDLIYIGVPKGKRIGEILHFLHQEVLHERVSNTKEALLSKAQAFLQTKKE

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=269, Percent_Identity=32.3420074349442, Blast_Score=105, Evalue=1e-22,
Organism=Escherichia coli, GI87081691, Length=237, Percent_Identity=32.9113924050633, Blast_Score=120, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI71995920, Length=432, Percent_Identity=25.6944444444444, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI281360112, Length=321, Percent_Identity=29.2834890965732, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI21357337, Length=321, Percent_Identity=29.2834890965732, Blast_Score=105, Evalue=7e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 53038; Mature: 53038

Theoretical pI: Translated: 7.99; Mature: 7.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCVILLCLFHCLDVLFYDLAYCVCDVGLRISILSKMKTHIFTPTLPKEVLYILESLQSAG
CHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCC
YEAYIVGGCVRDMLLAQNSHIPYVINDYDITTSALPQEVMKLFAHTIHTGLKYGTISVLI
CEEEEEHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
ESQSYEVTTFRIDGSYINARSPKNVTFTRSLAQDLQRRDFTINALAYAPCSGLIDEVGGL
ECCCEEEEEEEEECCEECCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHCCH
ADLQHKRIVCVGDAFARLNEDILRILRALRFSSTLGFEIDFHTKEAIFSLSSKVCLIAKE
HHCCCCEEEEECHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHCCCCEEEEHH
RIRVEFTKLLCGAWVKVVLDEFRFVISEILSQMREIIANNTLWQHTLSILDNLTPRSHTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHH
AWVAFLYHTDELKARDILQSLKFDTYNKDYICSLLVYVKKPLNNNLTTLKTMLVDMGGRK
HHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCC
KGEAMFKDMLKLKVAVINAQDTADKAQKLQMLDSIHSLLECVLASSVPLELKELCLNGRD
CHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCC
LIYIGVPKGKRIGEILHFLHQEVLHERVSNTKEALLSKAQAFLQTKKE
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MCVILLCLFHCLDVLFYDLAYCVCDVGLRISILSKMKTHIFTPTLPKEVLYILESLQSAG
CHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCC
YEAYIVGGCVRDMLLAQNSHIPYVINDYDITTSALPQEVMKLFAHTIHTGLKYGTISVLI
CEEEEEHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
ESQSYEVTTFRIDGSYINARSPKNVTFTRSLAQDLQRRDFTINALAYAPCSGLIDEVGGL
ECCCEEEEEEEEECCEECCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHCCH
ADLQHKRIVCVGDAFARLNEDILRILRALRFSSTLGFEIDFHTKEAIFSLSSKVCLIAKE
HHCCCCEEEEECHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHCCCCEEEEHH
RIRVEFTKLLCGAWVKVVLDEFRFVISEILSQMREIIANNTLWQHTLSILDNLTPRSHTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHH
AWVAFLYHTDELKARDILQSLKFDTYNKDYICSLLVYVKKPLNNNLTTLKTMLVDMGGRK
HHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCC
KGEAMFKDMLKLKVAVINAQDTADKAQKLQMLDSIHSLLECVLASSVPLELKELCLNGRD
CHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCC
LIYIGVPKGKRIGEILHFLHQEVLHERVSNTKEALLSKAQAFLQTKKE
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA