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The map label for this gene is dapF

Identifier: 32267200

GI number: 32267200

Start: 1627596

End: 1628390

Strand: Direct

Name: dapF

Synonym: HH1701

Alternate gene names: 32267200

Gene position: 1627596-1628390 (Clockwise)

Preceding gene: 32267199

Following gene: 32267203

Centisome position: 90.46

GC content: 36.35

Gene sequence:

>795_bases
ATGCGTTTTGTAAAATATAGTAGCAATGGCAATGATTTTCTTATTTTCACTACTCATACCTATGATGATGGCTCTCGCAC
TGCTTTAGCAAAAAGTGTTTGTGAGCGACATAATGGCATTGGTGCTGATGGTATGGTGGCAGTTATTCCTGCTATAAATA
AAGATTATGCCTATGAATGGGAATTCTATAATGCTGATGGCTCTGTTGCAAAAATGTGTGGTAATGCAAGTCGGAGTGTA
GGGCATTATGCGTATGTAGAGGGTATTGCTCAAAAACGACACTTTTTTTTAAGTAAAGTTGGACTTATTGGCATTACACT
AGATGATAACGACATTTCTTTAGTACAGAGTGATTTAGGTGAGTGTGCTTTATTGCAAGAAGATATTATAGAATCTAATC
CTTATGGTGTGAAATCGTGGAACTTGCTAGATTCTGGTGTGCCACATTTGGTGGGATTTGTTTCACAAGAGAATGCTTTG
CCTACACAAAAAGATAGATTGCTTGAAGATTTAAGACATAAATATAACGCAAATGTAAGTCTTGGCTTTATAGGAGAGGG
TGCGATAAGTTATAGCACCTATGAACGCGGTGTTGAGGATATTACTCAAGCGTGTGGCACAGGTGCGATTGCAGTATTTG
CTTTAGCTTTAAAACTTGGGTTATGTGAGAAATCTGCTATTCTTATTCCCCCAAGTAAGGAACTTTTGCACTTATATATG
AATGAAGATTCTCATATTTTATTTAAAGGTAAAGTAAAACGTATTGCTACTTGTGAATGGCTTTTAGAATCTTAA

Upstream 100 bases:

>100_bases
AATGAGGGTGATTTGCGCACTTTGCAACATAATGCAGAAACTTATATACAATCTTTACCTTGTGTAGATTGTGTGCAAAG
TTCTTAAAATGGAGGATTGT

Downstream 100 bases:

>100_bases
AGTGTAATTTACTCAAAAATTTCTTTAAAATACTCATTGCTTTGTGATTTGGGTATTGTCTTTGTCGTAGGAATTGCAAG
GACTTTATAAATTTTGGTAT

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MRFVKYSSNGNDFLIFTTHTYDDGSRTALAKSVCERHNGIGADGMVAVIPAINKDYAYEWEFYNADGSVAKMCGNASRSV
GHYAYVEGIAQKRHFFLSKVGLIGITLDDNDISLVQSDLGECALLQEDIIESNPYGVKSWNLLDSGVPHLVGFVSQENAL
PTQKDRLLEDLRHKYNANVSLGFIGEGAISYSTYERGVEDITQACGTGAIAVFALALKLGLCEKSAILIPPSKELLHLYM
NEDSHILFKGKVKRIATCEWLLES

Sequences:

>Translated_264_residues
MRFVKYSSNGNDFLIFTTHTYDDGSRTALAKSVCERHNGIGADGMVAVIPAINKDYAYEWEFYNADGSVAKMCGNASRSV
GHYAYVEGIAQKRHFFLSKVGLIGITLDDNDISLVQSDLGECALLQEDIIESNPYGVKSWNLLDSGVPHLVGFVSQENAL
PTQKDRLLEDLRHKYNANVSLGFIGEGAISYSTYERGVEDITQACGTGAIAVFALALKLGLCEKSAILIPPSKELLHLYM
NEDSHILFKGKVKRIATCEWLLES
>Mature_264_residues
MRFVKYSSNGNDFLIFTTHTYDDGSRTALAKSVCERHNGIGADGMVAVIPAINKDYAYEWEFYNADGSVAKMCGNASRSV
GHYAYVEGIAQKRHFFLSKVGLIGITLDDNDISLVQSDLGECALLQEDIIESNPYGVKSWNLLDSGVPHLVGFVSQENAL
PTQKDRLLEDLRHKYNANVSLGFIGEGAISYSTYERGVEDITQACGTGAIAVFALALKLGLCEKSAILIPPSKELLHLYM
NEDSHILFKGKVKRIATCEWLLES

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family [H]

Homologues:

Organism=Escherichia coli, GI87082334, Length=246, Percent_Identity=25.609756097561, Blast_Score=86, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001653
- InterPro:   IPR018510 [H]

Pfam domain/function: PF01678 DAP_epimerase [H]

EC number: =5.1.1.7 [H]

Molecular weight: Translated: 29053; Mature: 29053

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFVKYSSNGNDFLIFTTHTYDDGSRTALAKSVCERHNGIGADGMVAVIPAINKDYAYEW
CEEEEECCCCCEEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCEEEE
EFYNADGSVAKMCGNASRSVGHYAYVEGIAQKRHFFLSKVGLIGITLDDNDISLVQSDLG
EEECCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHC
ECALLQEDIIESNPYGVKSWNLLDSGVPHLVGFVSQENALPTQKDRLLEDLRHKYNANVS
HHHHHHHHHHCCCCCCCCCCCHHHCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCEE
LGFIGEGAISYSTYERGVEDITQACGTGAIAVFALALKLGLCEKSAILIPPSKELLHLYM
EEEECCCCEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEECCHHHHEEEEE
NEDSHILFKGKVKRIATCEWLLES
CCCCEEEEECCCCHHHHHHHHCCC
>Mature Secondary Structure
MRFVKYSSNGNDFLIFTTHTYDDGSRTALAKSVCERHNGIGADGMVAVIPAINKDYAYEW
CEEEEECCCCCEEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCEEEE
EFYNADGSVAKMCGNASRSVGHYAYVEGIAQKRHFFLSKVGLIGITLDDNDISLVQSDLG
EEECCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHC
ECALLQEDIIESNPYGVKSWNLLDSGVPHLVGFVSQENALPTQKDRLLEDLRHKYNANVS
HHHHHHHHHHCCCCCCCCCCCHHHCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCEE
LGFIGEGAISYSTYERGVEDITQACGTGAIAVFALALKLGLCEKSAILIPPSKELLHLYM
EEEECCCCEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEECCHHHHEEEEE
NEDSHILFKGKVKRIATCEWLLES
CCCCEEEEECCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA