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The map label for this gene is dnaB

Identifier: 32267157

GI number: 32267157

Start: 1585869

End: 1587332

Strand: Direct

Name: dnaB

Synonym: HH1658

Alternate gene names: 32267157

Gene position: 1585869-1587332 (Clockwise)

Preceding gene: 32267156

Following gene: 32267158

Centisome position: 88.15

GC content: 36.2

Gene sequence:

>1464_bases
ATGCAGCAACAAGAGCATATCATTAGTATTGAAAAAATTGTCCTTTCATCAATTTTTTTCTCTCCTGAAAAATTTGAGGA
AATCTCTGCTGAACTTCAAGCTGATGATTTTCTATATCCAGCACATCGTGATATTTTTGATATGTGTGTATATTTAAATA
AATGTAATCTTCCAATTGATACAGAGCTTATTCTTACTAGAAAACCATCACATAAAAAAATTTCACAAGAAGAACTACTT
GAGATTACAGCCCTTAATCCTATTGCCAATATTGAAGCGTATGTGAGCGAGATTAAAGAGGCGAGTATCAAGCGCGAGCT
TCATTCTTTGGCAAATTTTTTGCGTGAAAAATCACTTGATATGCAAGAGAGTGTAGAAGATATTATTGATGAAGTGGAGC
GTAAGCTCAATGATGTAGCACTTGGCAAACCCCAAGATAATGTTATGAATGCACACGAAATGGCAATAGGAGTTCTAGAA
CATCTCAAGGAGCTTAAAAATCGTGGTAATCAAGTTGTAGTAGGGCTTGACACGGGATTTAAAAGGCTTAATCTGCTGAC
AACAGGCTTTAATAAGGGAGATTTTATTATCATTGGCGCACGTCCTTCTATGGGAAAGACATCATTTGTGCTTAATCTTG
CTCAACATATTTTAGATTCTCCAAAAGGCGTAGCTTTTTTTAGCCTTGAAATGCCTTTTCTCCAGCTTGTTATGCGTATG
ATAAGTGCAAAGGCTTCTATTCATTTACAAAATCTACGAATAGGCAATCTTACTAATAATGAGTGGTCAAACCTGAGTGA
AACTATTGATGCATTTGCAAAGCAGCCTATATGGGTTGATGATAATTCAAATTTAAGTATTGCACAATTAAAAGGGCGTT
TGCGCCGATTAAAAAAACAGCACCCAGAAATTAGTATAGCAATCATTGATTATTTGCAGCTTATGAATGGCGGTAAAAAT
GAGGGTAGCAAACAAGCAGAAGTTTCTGAAATCTCGCGTGGGCTTAAAATGTTAGCTCGTGAGCTTGATATGCCAATCAT
TGCACTCTCACAGCTTAATCGCTTTGTGGAATCTCGTGATGATAAAAGACCTATTTTATCAGATTTGCGAGATTCTGGTG
CGATAGAGCAAGATGCAGATATTATTTTATTTTTATATCGTGAAGATGTGTATAAAAAACGTGCAGATAATGACAGATAT
GCGCGATTAAAAAAAGAAGGTAAGGAAAAAGATTTTAAACCAGAATATCAAAAACGCGAAATTGAGAAAGCTGAAATTAT
TATTGCCAAGAATCGTAATGGCGAAACAGGAAGCGTGGAGATTCAATTTGATACGCGCTTTACACGTTTTGAAGATGTGC
CCGAAAAGTATGAGCACGAGATGACACAAACGCGCATTGATGGAGAAGAGTTTATGACACAAATAGAGAATCTGCCTCCA
CAAGTCCAAATGCCAAATATTTGA

Upstream 100 bases:

>100_bases
ATACGAATAAGCACATTTAATTCAATACAAATTACTCTTTAAATAAAAACAAGGTAAGATTATGGAATCTAAAATCATTT
GAACAATAGGGATATAAAAG

Downstream 100 bases:

>100_bases
TGAATTTAAGTGAAAGATTCTCATAAAAATACACATTCACTTTTTGAAGTTGAACTTTTTAGCACAAGAAGACAATGGGC
AGTATTTATATGTATTTGCG

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 487; Mature: 487

Protein sequence:

>487_residues
MQQQEHIISIEKIVLSSIFFSPEKFEEISAELQADDFLYPAHRDIFDMCVYLNKCNLPIDTELILTRKPSHKKISQEELL
EITALNPIANIEAYVSEIKEASIKRELHSLANFLREKSLDMQESVEDIIDEVERKLNDVALGKPQDNVMNAHEMAIGVLE
HLKELKNRGNQVVVGLDTGFKRLNLLTTGFNKGDFIIIGARPSMGKTSFVLNLAQHILDSPKGVAFFSLEMPFLQLVMRM
ISAKASIHLQNLRIGNLTNNEWSNLSETIDAFAKQPIWVDDNSNLSIAQLKGRLRRLKKQHPEISIAIIDYLQLMNGGKN
EGSKQAEVSEISRGLKMLARELDMPIIALSQLNRFVESRDDKRPILSDLRDSGAIEQDADIILFLYREDVYKKRADNDRY
ARLKKEGKEKDFKPEYQKREIEKAEIIIAKNRNGETGSVEIQFDTRFTRFEDVPEKYEHEMTQTRIDGEEFMTQIENLPP
QVQMPNI

Sequences:

>Translated_487_residues
MQQQEHIISIEKIVLSSIFFSPEKFEEISAELQADDFLYPAHRDIFDMCVYLNKCNLPIDTELILTRKPSHKKISQEELL
EITALNPIANIEAYVSEIKEASIKRELHSLANFLREKSLDMQESVEDIIDEVERKLNDVALGKPQDNVMNAHEMAIGVLE
HLKELKNRGNQVVVGLDTGFKRLNLLTTGFNKGDFIIIGARPSMGKTSFVLNLAQHILDSPKGVAFFSLEMPFLQLVMRM
ISAKASIHLQNLRIGNLTNNEWSNLSETIDAFAKQPIWVDDNSNLSIAQLKGRLRRLKKQHPEISIAIIDYLQLMNGGKN
EGSKQAEVSEISRGLKMLARELDMPIIALSQLNRFVESRDDKRPILSDLRDSGAIEQDADIILFLYREDVYKKRADNDRY
ARLKKEGKEKDFKPEYQKREIEKAEIIIAKNRNGETGSVEIQFDTRFTRFEDVPEKYEHEMTQTRIDGEEFMTQIENLPP
QVQMPNI
>Mature_487_residues
MQQQEHIISIEKIVLSSIFFSPEKFEEISAELQADDFLYPAHRDIFDMCVYLNKCNLPIDTELILTRKPSHKKISQEELL
EITALNPIANIEAYVSEIKEASIKRELHSLANFLREKSLDMQESVEDIIDEVERKLNDVALGKPQDNVMNAHEMAIGVLE
HLKELKNRGNQVVVGLDTGFKRLNLLTTGFNKGDFIIIGARPSMGKTSFVLNLAQHILDSPKGVAFFSLEMPFLQLVMRM
ISAKASIHLQNLRIGNLTNNEWSNLSETIDAFAKQPIWVDDNSNLSIAQLKGRLRRLKKQHPEISIAIIDYLQLMNGGKN
EGSKQAEVSEISRGLKMLARELDMPIIALSQLNRFVESRDDKRPILSDLRDSGAIEQDADIILFLYREDVYKKRADNDRY
ARLKKEGKEKDFKPEYQKREIEKAEIIIAKNRNGETGSVEIQFDTRFTRFEDVPEKYEHEMTQTRIDGEEFMTQIENLPP
QVQMPNI

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=465, Percent_Identity=35.6989247311828, Blast_Score=271, Evalue=8e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 55929; Mature: 55929

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQQEHIISIEKIVLSSIFFSPEKFEEISAELQADDFLYPAHRDIFDMCVYLNKCNLPID
CCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCC
TELILTRKPSHKKISQEELLEITALNPIANIEAYVSEIKEASIKRELHSLANFLREKSLD
CEEEEECCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
MQESVEDIIDEVERKLNDVALGKPQDNVMNAHEMAIGVLEHLKELKNRGNQVVVGLDTGF
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCC
KRLNLLTTGFNKGDFIIIGARPSMGKTSFVLNLAQHILDSPKGVAFFSLEMPFLQLVMRM
HHHHHEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHH
ISAKASIHLQNLRIGNLTNNEWSNLSETIDAFAKQPIWVDDNSNLSIAQLKGRLRRLKKQ
HHHHHHEEEEEEEECCCCCCCHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHH
HPEISIAIIDYLQLMNGGKNEGSKQAEVSEISRGLKMLARELDMPIIALSQLNRFVESRD
CCCEEEHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCC
DKRPILSDLRDSGAIEQDADIILFLYREDVYKKRADNDRYARLKKEGKEKDFKPEYQKRE
CCCHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHCCCHHHHHHHHHCCCCCCCCCHHHHH
IEKAEIIIAKNRNGETGSVEIQFDTRFTRFEDVPEKYEHEMTQTRIDGEEFMTQIENLPP
CCCEEEEEEECCCCCCCEEEEEECCCHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHCCCC
QVQMPNI
CCCCCCC
>Mature Secondary Structure
MQQQEHIISIEKIVLSSIFFSPEKFEEISAELQADDFLYPAHRDIFDMCVYLNKCNLPID
CCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCC
TELILTRKPSHKKISQEELLEITALNPIANIEAYVSEIKEASIKRELHSLANFLREKSLD
CEEEEECCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
MQESVEDIIDEVERKLNDVALGKPQDNVMNAHEMAIGVLEHLKELKNRGNQVVVGLDTGF
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCC
KRLNLLTTGFNKGDFIIIGARPSMGKTSFVLNLAQHILDSPKGVAFFSLEMPFLQLVMRM
HHHHHEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHH
ISAKASIHLQNLRIGNLTNNEWSNLSETIDAFAKQPIWVDDNSNLSIAQLKGRLRRLKKQ
HHHHHHEEEEEEEECCCCCCCHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHH
HPEISIAIIDYLQLMNGGKNEGSKQAEVSEISRGLKMLARELDMPIIALSQLNRFVESRD
CCCEEEHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCC
DKRPILSDLRDSGAIEQDADIILFLYREDVYKKRADNDRYARLKKEGKEKDFKPEYQKRE
CCCHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHCCCHHHHHHHHHCCCCCCCCCHHHHH
IEKAEIIIAKNRNGETGSVEIQFDTRFTRFEDVPEKYEHEMTQTRIDGEEFMTQIENLPP
CCCEEEEEEECCCCCCCEEEEEECCCHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHCCCC
QVQMPNI
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9252185 [H]