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The map label for this gene is purC

Identifier: 32267118

GI number: 32267118

Start: 1542780

End: 1543490

Strand: Direct

Name: purC

Synonym: HH1619

Alternate gene names: 32267118

Gene position: 1542780-1543490 (Clockwise)

Preceding gene: 32267117

Following gene: 32267119

Centisome position: 85.75

GC content: 34.74

Gene sequence:

>711_bases
ATGGAAAAACGAGAAATGCTATATGAAGGGAAGGGTAAAAAGCTTTTTGCCACGGATGATGAACATATTGTAATTGCAGA
GTTTAAAGATGATTTAACGGCTTTTAACGCAGAAAAAAAAGGTAAGGAATCGGGCAAAGGTGAGCTAAATTGTCAAATTA
GTTCAGTTCTTTTTGAGCTTTTAGCACGGCATAATATAGCTACACATTATATCAAGCGTCTTAATATGAGTGATATGTTG
TGTAAAAAAGTAACTATTATTCCTATTGAAGTGGTTGTGCGTAATATTGCTACAGGTTCGCTGACAAAGCGTTTAGGCAT
AAGTGATGGAAGTATTCTTTCCCAGACGCTTGTTGAATTTTATTATAAAGATGATGCACTTGGCGACCCATTAATTAATG
ATGAGCATTGTAAGATTTTGGGCATTATAAAGGAGCAAAGTCAGTTAGAGATACTTAAGAGCCAAGCGCGTCAAATAAAT
GAAATTTTGAAAGCATTTTTTGATGAAAAAGGTTTGCGACTCGTTGATTTTAAGCTTGAATTTGGACAAGATAGCGCTGG
AAATATTATACTTGCTGATGAAATTAGTCCTGATAGTTGCCGTTTATGGGATAAACAAACAAATCAAAAACTTGATAAAG
ATAGATTTAGGGAGAATCTAGGCAGCGTAAAAGTTGCCTATGAAGAAGTATTACGGCGGATTTTAGTATAG

Upstream 100 bases:

>100_bases
GATATATCAAGATATTCAACTTAAAAGTGCTATTGATGTGCTTAAAGCGTGGGGTGTGATTGGTGCTTTAAATCCAAACA
AGTAATAAGGAGTTAAATCA

Downstream 100 bases:

>100_bases
AGGATAAAAATGAAGGTAAAAGTTCTTGTTTCACTTAAAGAGGGGGTGCTTGACCCTCAAGCAAAGGCTATTGCTCACGC
ATTGTGTGCTCACGGATTTG

Product: phosphoribosylaminoimidazole-succinocarboxamide synthase

Products: NA

Alternate protein names: SAICAR synthetase

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MEKREMLYEGKGKKLFATDDEHIVIAEFKDDLTAFNAEKKGKESGKGELNCQISSVLFELLARHNIATHYIKRLNMSDML
CKKVTIIPIEVVVRNIATGSLTKRLGISDGSILSQTLVEFYYKDDALGDPLINDEHCKILGIIKEQSQLEILKSQARQIN
EILKAFFDEKGLRLVDFKLEFGQDSAGNIILADEISPDSCRLWDKQTNQKLDKDRFRENLGSVKVAYEEVLRRILV

Sequences:

>Translated_236_residues
MEKREMLYEGKGKKLFATDDEHIVIAEFKDDLTAFNAEKKGKESGKGELNCQISSVLFELLARHNIATHYIKRLNMSDML
CKKVTIIPIEVVVRNIATGSLTKRLGISDGSILSQTLVEFYYKDDALGDPLINDEHCKILGIIKEQSQLEILKSQARQIN
EILKAFFDEKGLRLVDFKLEFGQDSAGNIILADEISPDSCRLWDKQTNQKLDKDRFRENLGSVKVAYEEVLRRILV
>Mature_236_residues
MEKREMLYEGKGKKLFATDDEHIVIAEFKDDLTAFNAEKKGKESGKGELNCQISSVLFELLARHNIATHYIKRLNMSDML
CKKVTIIPIEVVVRNIATGSLTKRLGISDGSILSQTLVEFYYKDDALGDPLINDEHCKILGIIKEQSQLEILKSQARQIN
EILKAFFDEKGLRLVDFKLEFGQDSAGNIILADEISPDSCRLWDKQTNQKLDKDRFRENLGSVKVAYEEVLRRILV

Specific function: De novo purine biosynthesis; seventh step. [C]

COG id: COG0152

COG function: function code F; Phosphoribosylaminoimidazolesuccinocarboxamide (SAICAR) synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SAICAR synthetase family

Homologues:

Organism=Homo sapiens, GI119220557, Length=245, Percent_Identity=35.1020408163265, Blast_Score=124, Evalue=8e-29,
Organism=Homo sapiens, GI5453539, Length=245, Percent_Identity=35.1020408163265, Blast_Score=124, Evalue=8e-29,
Organism=Homo sapiens, GI119220559, Length=252, Percent_Identity=34.1269841269841, Blast_Score=117, Evalue=7e-27,
Organism=Escherichia coli, GI1788820, Length=236, Percent_Identity=40.6779661016949, Blast_Score=188, Evalue=3e-49,
Organism=Caenorhabditis elegans, GI17531275, Length=225, Percent_Identity=33.7777777777778, Blast_Score=118, Evalue=2e-27,
Organism=Drosophila melanogaster, GI18860083, Length=237, Percent_Identity=32.0675105485232, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24583917, Length=208, Percent_Identity=31.25, Blast_Score=96, Evalue=2e-20,

Paralogues:

None

Copy number: 1680 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 13,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PUR7_HELHP (Q7VFQ6)

Other databases:

- EMBL:   AE017125
- RefSeq:   NP_861150.1
- ProteinModelPortal:   Q7VFQ6
- SMR:   Q7VFQ6
- GeneID:   1492166
- GenomeReviews:   AE017125_GR
- KEGG:   hhe:HH1619
- NMPDR:   fig|235279.1.peg.1619
- HOGENOM:   HBG306070
- OMA:   YKDDALG
- ProtClustDB:   PRK09362
- BioCyc:   HHEP235279:HH_1619-MONOMER
- BRENDA:   6.3.2.6
- HAMAP:   MF_00137
- InterPro:   IPR013816
- InterPro:   IPR001636
- InterPro:   IPR018236
- Gene3D:   G3DSA:3.30.470.20
- PANTHER:   PTHR11609
- TIGRFAMs:   TIGR00081

Pfam domain/function: PF01259 SAICAR_synt

EC number: =6.3.2.6

Molecular weight: Translated: 26895; Mature: 26895

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS01057 SAICAR_SYNTHETASE_1; PS01058 SAICAR_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKREMLYEGKGKKLFATDDEHIVIAEFKDDLTAFNAEKKGKESGKGELNCQISSVLFEL
CCCHHHHHCCCCCEEEECCCCEEEEEECCCHHHHHCCHHCCCCCCCCCEEEEHHHHHHHH
LARHNIATHYIKRLNMSDMLCKKVTIIPIEVVVRNIATGSLTKRLGISDGSILSQTLVEF
HHHCCHHHHHHHHCCHHHHHHHHHHEEHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHH
YYKDDALGDPLINDEHCKILGIIKEQSQLEILKSQARQINEILKAFFDEKGLRLVDFKLE
HHCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEE
FGQDSAGNIILADEISPDSCRLWDKQTNQKLDKDRFRENLGSVKVAYEEVLRRILV
CCCCCCCCEEEEECCCCCCHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MEKREMLYEGKGKKLFATDDEHIVIAEFKDDLTAFNAEKKGKESGKGELNCQISSVLFEL
CCCHHHHHCCCCCEEEECCCCEEEEEECCCHHHHHCCHHCCCCCCCCCEEEEHHHHHHHH
LARHNIATHYIKRLNMSDMLCKKVTIIPIEVVVRNIATGSLTKRLGISDGSILSQTLVEF
HHHCCHHHHHHHHCCHHHHHHHHHHEEHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHH
YYKDDALGDPLINDEHCKILGIIKEQSQLEILKSQARQINEILKAFFDEKGLRLVDFKLE
HHCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEE
FGQDSAGNIILADEISPDSCRLWDKQTNQKLDKDRFRENLGSVKVAYEEVLRRILV
CCCCCCCCEEEEECCCCCCHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12810954