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The map label for this gene is miaB

Identifier: 32266773

GI number: 32266773

Start: 1237945

End: 1239276

Strand: Direct

Name: miaB

Synonym: HH1274

Alternate gene names: 32266773

Gene position: 1237945-1239276 (Clockwise)

Preceding gene: 32266772

Following gene: 32266774

Centisome position: 68.81

GC content: 36.86

Gene sequence:

>1332_bases
ATGATTGCCGAATTACGCGATAAAAAACATTATACTCTTACAAATGATATTAAACAAGCAGATTTGATTCTTATTAATAC
TTGCTCTGTGCGCGAAAAACCAGAAAAAAAGCTTTTTTCTGAAATTGGAGCATTTGCCAAAGAAAAAAAAGCAGGGGCAA
AAATCGGTGTTTGTGGTTGCACAGCCAGTCATTTGGGTGAGGAAATTATCAAAAAAGCTCCAAGTGTAGATTTTGTATTA
GGTGCAAGAAATGTATCAAAAATAACGCAAGTGCTTGAGCGTCCCAAAGCCGTTGAGGTAGATATTGATTATGATGATAG
CACTTATGTGTTTGCCTCTTCACAAGGTATGGGTATCAAAGCGCATCTTAACATCTCCATAGGTTGTGATAAAAAATGTA
GTTATTGCATTGTGCCCTTTACGCGTGGTAAAGAGATTTCAGTTCCTAAAGATTTGCTTATATCTGAAGCAAAAAAATGT
GTGGCAAGTGGTGCGAAAGAATTACTTTTATTAGGGCAAAATGTTAATAATTATGGCGTGAGATTTTCACACTCTCACCC
AAAAACAAATTTTACGCAGCTTTTACGCGCTTTGAGCGAGATTGATGGTTTATACAGAATCCGTTTTACTTCTCCACACC
CATTGCATATGGACGATGAATTTTTAGAGGAATTTGCAAGTAATCCTGTAATTGCCAAAGGCATACATATTCCATTACAA
AGTGGTTCAAGCCAGATTCTCAAAATGATGAGAAGAGGTTATGATAAGCAATGGTATCTTAACAGAATTGCTAAACTTAA
GTCCCTTGTCCCTAATGTTGGCATTGGCACAGATATTATTGTGGGATTTCCTACCGAATCCGAGCAAGACTTTGAAGACA
CGATGGAAGTACTTTCTCTTGTAGAGTTTGATACGCTTTATAGTTTTGTATATTCGCCTCGTCCGCATACAAGTGCGTTT
GAATATGACAAAAGTATGCTTGTGTCTCCTGAAGTGGCAAAAGAGCGTTTAGCACGATTGCAAAATCTTCATAAAGAGAT
TCTTAGCAAAAAAGCACAACTTGAAATTGGACGCATTCATAATGTGCTTATTGAAAATCACTACAATGGTGAGGGGCAGT
GTTGGAGTGAGGGGCGTAGTAGTAGCAATAAGCTGATTAAAATTTTAGATAAAAAATGTGAGATTGGTTCTATCGTAAAA
GTAGAGATAACACACAATGAAGGTGGTGGGCTTATGGGGAGATTTATAAATGAACTAAGTCTTAGCGAAGCACTTGCTTC
TAAGGAATATTTTCAAAATCCTATATACATTCAAGAAGGTGCCTTATGCTAA

Upstream 100 bases:

>100_bases
ACTTCATATTATCTAAAATCATAGAATCTATACACTTGAGACAATGAAACTTTTTATACAAACATTGGGTTGTGCAATGA
ATGAGCGCGATTCTGCGCAT

Downstream 100 bases:

>100_bases
GTCGTGAGAGAAAACGTCAGATACTTATTAAAATCGCTCCACCCATTATGTATGCTGCATTGCGTATTTTATATGCTCTT
ACACGACATAGATTTCACAT

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 443; Mature: 443

Protein sequence:

>443_residues
MIAELRDKKHYTLTNDIKQADLILINTCSVREKPEKKLFSEIGAFAKEKKAGAKIGVCGCTASHLGEEIIKKAPSVDFVL
GARNVSKITQVLERPKAVEVDIDYDDSTYVFASSQGMGIKAHLNISIGCDKKCSYCIVPFTRGKEISVPKDLLISEAKKC
VASGAKELLLLGQNVNNYGVRFSHSHPKTNFTQLLRALSEIDGLYRIRFTSPHPLHMDDEFLEEFASNPVIAKGIHIPLQ
SGSSQILKMMRRGYDKQWYLNRIAKLKSLVPNVGIGTDIIVGFPTESEQDFEDTMEVLSLVEFDTLYSFVYSPRPHTSAF
EYDKSMLVSPEVAKERLARLQNLHKEILSKKAQLEIGRIHNVLIENHYNGEGQCWSEGRSSSNKLIKILDKKCEIGSIVK
VEITHNEGGGLMGRFINELSLSEALASKEYFQNPIYIQEGALC

Sequences:

>Translated_443_residues
MIAELRDKKHYTLTNDIKQADLILINTCSVREKPEKKLFSEIGAFAKEKKAGAKIGVCGCTASHLGEEIIKKAPSVDFVL
GARNVSKITQVLERPKAVEVDIDYDDSTYVFASSQGMGIKAHLNISIGCDKKCSYCIVPFTRGKEISVPKDLLISEAKKC
VASGAKELLLLGQNVNNYGVRFSHSHPKTNFTQLLRALSEIDGLYRIRFTSPHPLHMDDEFLEEFASNPVIAKGIHIPLQ
SGSSQILKMMRRGYDKQWYLNRIAKLKSLVPNVGIGTDIIVGFPTESEQDFEDTMEVLSLVEFDTLYSFVYSPRPHTSAF
EYDKSMLVSPEVAKERLARLQNLHKEILSKKAQLEIGRIHNVLIENHYNGEGQCWSEGRSSSNKLIKILDKKCEIGSIVK
VEITHNEGGGLMGRFINELSLSEALASKEYFQNPIYIQEGALC
>Mature_443_residues
MIAELRDKKHYTLTNDIKQADLILINTCSVREKPEKKLFSEIGAFAKEKKAGAKIGVCGCTASHLGEEIIKKAPSVDFVL
GARNVSKITQVLERPKAVEVDIDYDDSTYVFASSQGMGIKAHLNISIGCDKKCSYCIVPFTRGKEISVPKDLLISEAKKC
VASGAKELLLLGQNVNNYGVRFSHSHPKTNFTQLLRALSEIDGLYRIRFTSPHPLHMDDEFLEEFASNPVIAKGIHIPLQ
SGSSQILKMMRRGYDKQWYLNRIAKLKSLVPNVGIGTDIIVGFPTESEQDFEDTMEVLSLVEFDTLYSFVYSPRPHTSAF
EYDKSMLVSPEVAKERLARLQNLHKEILSKKAQLEIGRIHNVLIENHYNGEGQCWSEGRSSSNKLIKILDKKCEIGSIVK
VEITHNEGGGLMGRFINELSLSEALASKEYFQNPIYIQEGALC

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=392, Percent_Identity=35.4591836734694, Blast_Score=216, Evalue=3e-56,
Organism=Homo sapiens, GI28872782, Length=392, Percent_Identity=35.4591836734694, Blast_Score=216, Evalue=5e-56,
Organism=Homo sapiens, GI93277076, Length=354, Percent_Identity=28.2485875706215, Blast_Score=125, Evalue=8e-29,
Organism=Escherichia coli, GI1786882, Length=423, Percent_Identity=34.9881796690307, Blast_Score=254, Evalue=8e-69,
Organism=Escherichia coli, GI1787057, Length=435, Percent_Identity=26.8965517241379, Blast_Score=150, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17553146, Length=439, Percent_Identity=29.6127562642369, Blast_Score=181, Evalue=6e-46,
Organism=Caenorhabditis elegans, GI71996771, Length=287, Percent_Identity=29.9651567944251, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI21356207, Length=414, Percent_Identity=32.3671497584541, Blast_Score=209, Evalue=4e-54,
Organism=Drosophila melanogaster, GI19922432, Length=420, Percent_Identity=26.9047619047619, Blast_Score=139, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_HELHP (Q7VGP6)

Other databases:

- EMBL:   AE017125
- RefSeq:   NP_860805.1
- GeneID:   1492962
- GenomeReviews:   AE017125_GR
- KEGG:   hhe:HH1274
- NMPDR:   fig|235279.1.peg.1274
- HOGENOM:   HBG457663
- OMA:   EVRTYGC
- ProtClustDB:   PRK14339
- BioCyc:   HHEP235279:HH_1274-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 49547; Mature: 49547

Theoretical pI: Translated: 7.74; Mature: 7.74

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAELRDKKHYTLTNDIKQADLILINTCSVREKPEKKLFSEIGAFAKEKKAGAKIGVCGC
CCCCCCCCCCEEECCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECC
TASHLGEEIIKKAPSVDFVLGARNVSKITQVLERPKAVEVDIDYDDSTYVFASSQGMGIK
CHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHCCCEEEEEECCCCCEEEEECCCCCEEE
AHLNISIGCDKKCSYCIVPFTRGKEISVPKDLLISEAKKCVASGAKELLLLGQNVNNYGV
EEEEEEECCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCE
RFSHSHPKTNFTQLLRALSEIDGLYRIRFTSPHPLHMDDEFLEEFASNPVIAKGIHIPLQ
EEECCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHCCCCEEECCEEEECC
SGSSQILKMMRRGYDKQWYLNRIAKLKSLVPNVGIGTDIIVGFPTESEQDFEDTMEVLSL
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHHHHHHHHH
VEFDTLYSFVYSPRPHTSAFEYDKSMLVSPEVAKERLARLQNLHKEILSKKAQLEIGRIH
HHHHHHHHHHCCCCCCCCHHHCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NVLIENHYNGEGQCWSEGRSSSNKLIKILDKKCEIGSIVKVEITHNEGGGLMGRFINELS
HHHEECCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEEEEECCCCCHHHHHHHHHH
LSEALASKEYFQNPIYIQEGALC
HHHHHHHHHHHCCCEEEECCCCC
>Mature Secondary Structure
MIAELRDKKHYTLTNDIKQADLILINTCSVREKPEKKLFSEIGAFAKEKKAGAKIGVCGC
CCCCCCCCCCEEECCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECC
TASHLGEEIIKKAPSVDFVLGARNVSKITQVLERPKAVEVDIDYDDSTYVFASSQGMGIK
CHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHCCCEEEEEECCCCCEEEEECCCCCEEE
AHLNISIGCDKKCSYCIVPFTRGKEISVPKDLLISEAKKCVASGAKELLLLGQNVNNYGV
EEEEEEECCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCE
RFSHSHPKTNFTQLLRALSEIDGLYRIRFTSPHPLHMDDEFLEEFASNPVIAKGIHIPLQ
EEECCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHCCCCEEECCEEEECC
SGSSQILKMMRRGYDKQWYLNRIAKLKSLVPNVGIGTDIIVGFPTESEQDFEDTMEVLSL
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHHHHHHHHH
VEFDTLYSFVYSPRPHTSAFEYDKSMLVSPEVAKERLARLQNLHKEILSKKAQLEIGRIH
HHHHHHHHHHCCCCCCCCHHHCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NVLIENHYNGEGQCWSEGRSSSNKLIKILDKKCEIGSIVKVEITHNEGGGLMGRFINELS
HHHEECCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEEEEECCCCCHHHHHHHHHH
LSEALASKEYFQNPIYIQEGALC
HHHHHHHHHHHCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12810954