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The map label for this gene is gdhA

Identifier: 32266740

GI number: 32266740

Start: 1204978

End: 1206330

Strand: Direct

Name: gdhA

Synonym: HH1241

Alternate gene names: 32266740

Gene position: 1204978-1206330 (Clockwise)

Preceding gene: 32266739

Following gene: 32266742

Centisome position: 66.97

GC content: 39.25

Gene sequence:

>1353_bases
ATGTCATACGCAAGTAATGTTCTTAACAAACTTAAGGAACAATATCCTTCACAAACGCTTTTTCATCAAGCTGTGGCGGA
AGTTTTTGAATCTCTAGAACCGGCTCTTCAAAAGGATAAGAAATATGAAACTTATGCGATACTTGAGCGTCTCACAATAC
CAGATAGAGAAATCCATTTTCGTGTAAGTTGGACTGATGATAATGGTAAAATCCAAACAAACCGAGCTTATCGTATTGAA
TTTAATGCTGCTATTGGACCATATAAAGGTGGTTTGCGTTTTCACCCAAGTGTAAATGAAGGTGTAATTAAATTTTTAGG
ATTTGAGCAGATTCTAAAAAACTCTCTTACAACTCTCGCTATGGGAGGTGGTAAGGGCGGAAGTGATTTTGACCCAAAAG
GAAAGAGTGAAGGCGAGATAATGCGATTTTGTCAAGCTTTTATGAATGAGCTTTATCGTCATATCGGTGCAACCACTGAT
GTTCCAGCAGGAGATATTGGCGTAGGTGGTAGAGAAATAGGCTATCTTTTTGGACAATATCGTAAATTAACAAACTGCTT
TGAAGGTGTCTTTACAGGTAAGGCAATTCCTTGGGGCGGAAGTCTTGTGCGCACGGAAGCAACAGGTTATGGTTGTGTAT
ATTTTGCTCAAGAAATGCTTAAAGATAGAGGTGAAAGTTTAGAGGGCAAAGTTTGTAGCGTTTCAGGTGCGGGTAATGTT
GCTATTTATACGATTGAAAAACTCCTTCAGCTTGGGGCAATTCCTGTTACTGCAAGTGATTCTCAAGGTATGATTTATGA
TAAAGAGGGTATTGATTTAGCATTACTCAAAGAAGTCAAAGAAATCAAACGTTTAACACTTGCAGAATATGCAAAAGCAA
GACCTCAAGCGGTTTATACAAAAGTGGCTGATTATCCAAAAGATAGCAATCCTGTATGGGCTATCCCTTGTTTTGCGGCA
TTCCCAAGTGCAACACAAAATGAACTCAATGGGAATGATGCTAAAACACTTCTTGCGAATGGTTGCAAATGTGTCAGTGA
AGGGGCAAATATGCCTTCAACCATTGAAGCTGTTCATCAATTTCTTGATGCAAAAATCTGCTATGGACCAGGTAAGGCTG
CAAATGCAGGTGGTGTAGCAGTCAGTGGTTTGGAAATGGCACAAAATGCAAGTATGAATCCGTGGACTTTTGAAGTTGTG
GATAAACGCTTACATACTATTATGGAAAGCATTTATGCAAATGCAAGTGAAACTGCAAAAGAATTTGGTGAGCCTACAAA
CTTAGTGCTTGGTGCAAATATTGCAGGATTCCGCAAGGTTGCTGGAGCAATGATAGACCAAGGTGTGATTTAA

Upstream 100 bases:

>100_bases
ATTAAACTTAATTAAGAATATTCAAGTTTAATTGGCTTATAATTGCAATGCGTTTAACGCACATCTTAAAATTAAAATAT
CCAAAAAAGGAGGCACCATT

Downstream 100 bases:

>100_bases
TTTGGCATTGAGTTACAATCCTAATAAAGAAGTTGAGTATTCTAAAGATGCGCAACTTCTTTTCTTAATTTCTAGCGATG
AATATTAATGTATTTGTGCA

Product: glutamate dehydrogenase

Products: NA

Alternate protein names: NADP-GDH [H]

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MSYASNVLNKLKEQYPSQTLFHQAVAEVFESLEPALQKDKKYETYAILERLTIPDREIHFRVSWTDDNGKIQTNRAYRIE
FNAAIGPYKGGLRFHPSVNEGVIKFLGFEQILKNSLTTLAMGGGKGGSDFDPKGKSEGEIMRFCQAFMNELYRHIGATTD
VPAGDIGVGGREIGYLFGQYRKLTNCFEGVFTGKAIPWGGSLVRTEATGYGCVYFAQEMLKDRGESLEGKVCSVSGAGNV
AIYTIEKLLQLGAIPVTASDSQGMIYDKEGIDLALLKEVKEIKRLTLAEYAKARPQAVYTKVADYPKDSNPVWAIPCFAA
FPSATQNELNGNDAKTLLANGCKCVSEGANMPSTIEAVHQFLDAKICYGPGKAANAGGVAVSGLEMAQNASMNPWTFEVV
DKRLHTIMESIYANASETAKEFGEPTNLVLGANIAGFRKVAGAMIDQGVI

Sequences:

>Translated_450_residues
MSYASNVLNKLKEQYPSQTLFHQAVAEVFESLEPALQKDKKYETYAILERLTIPDREIHFRVSWTDDNGKIQTNRAYRIE
FNAAIGPYKGGLRFHPSVNEGVIKFLGFEQILKNSLTTLAMGGGKGGSDFDPKGKSEGEIMRFCQAFMNELYRHIGATTD
VPAGDIGVGGREIGYLFGQYRKLTNCFEGVFTGKAIPWGGSLVRTEATGYGCVYFAQEMLKDRGESLEGKVCSVSGAGNV
AIYTIEKLLQLGAIPVTASDSQGMIYDKEGIDLALLKEVKEIKRLTLAEYAKARPQAVYTKVADYPKDSNPVWAIPCFAA
FPSATQNELNGNDAKTLLANGCKCVSEGANMPSTIEAVHQFLDAKICYGPGKAANAGGVAVSGLEMAQNASMNPWTFEVV
DKRLHTIMESIYANASETAKEFGEPTNLVLGANIAGFRKVAGAMIDQGVI
>Mature_449_residues
SYASNVLNKLKEQYPSQTLFHQAVAEVFESLEPALQKDKKYETYAILERLTIPDREIHFRVSWTDDNGKIQTNRAYRIEF
NAAIGPYKGGLRFHPSVNEGVIKFLGFEQILKNSLTTLAMGGGKGGSDFDPKGKSEGEIMRFCQAFMNELYRHIGATTDV
PAGDIGVGGREIGYLFGQYRKLTNCFEGVFTGKAIPWGGSLVRTEATGYGCVYFAQEMLKDRGESLEGKVCSVSGAGNVA
IYTIEKLLQLGAIPVTASDSQGMIYDKEGIDLALLKEVKEIKRLTLAEYAKARPQAVYTKVADYPKDSNPVWAIPCFAAF
PSATQNELNGNDAKTLLANGCKCVSEGANMPSTIEAVHQFLDAKICYGPGKAANAGGVAVSGLEMAQNASMNPWTFEVVD
KRLHTIMESIYANASETAKEFGEPTNLVLGANIAGFRKVAGAMIDQGVI

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=337, Percent_Identity=28.1899109792285, Blast_Score=125, Evalue=7e-29,
Organism=Homo sapiens, GI4885281, Length=360, Percent_Identity=28.6111111111111, Blast_Score=121, Evalue=1e-27,
Organism=Escherichia coli, GI1788059, Length=446, Percent_Identity=57.6233183856502, Blast_Score=495, Evalue=1e-141,
Organism=Caenorhabditis elegans, GI17544676, Length=369, Percent_Identity=27.6422764227642, Blast_Score=108, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6319256, Length=467, Percent_Identity=49.4646680942184, Blast_Score=383, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6324951, Length=455, Percent_Identity=48.1318681318681, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI45553475, Length=414, Percent_Identity=27.7777777777778, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI45549226, Length=389, Percent_Identity=28.2776349614396, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24649283, Length=424, Percent_Identity=27.3584905660377, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24649460, Length=292, Percent_Identity=29.1095890410959, Blast_Score=101, Evalue=1e-21,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.4 [H]

Molecular weight: Translated: 48921; Mature: 48790

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYASNVLNKLKEQYPSQTLFHQAVAEVFESLEPALQKDKKYETYAILERLTIPDREIHF
CCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEE
RVSWTDDNGKIQTNRAYRIEFNAAIGPYKGGLRFHPSVNEGVIKFLGFEQILKNSLTTLA
EEEEECCCCEEEECCEEEEEEECCCCCCCCCEEECCCCCHHHHHHHHHHHHHHCCHHEEE
MGGGKGGSDFDPKGKSEGEIMRFCQAFMNELYRHIGATTDVPAGDIGVGGREIGYLFGQY
ECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHH
RKLTNCFEGVFTGKAIPWGGSLVRTEATGYGCVYFAQEMLKDRGESLEGKVCSVSGAGNV
HHHHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCCCE
AIYTIEKLLQLGAIPVTASDSQGMIYDKEGIDLALLKEVKEIKRLTLAEYAKARPQAVYT
EEHHHHHHHHHCCCEEECCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHH
KVADYPKDSNPVWAIPCFAAFPSATQNELNGNDAKTLLANGCKCVSEGANMPSTIEAVHQ
HHHCCCCCCCCEEEEEHHHHCCCCCCCCCCCCHHHHHHHCCCHHHHCCCCCCHHHHHHHH
FLDAKICYGPGKAANAGGVAVSGLEMAQNASMNPWTFEVVDKRLHTIMESIYANASETAK
HHCCEEEECCCCCCCCCCEEEEHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHH
EFGEPTNLVLGANIAGFRKVAGAMIDQGVI
HHCCCCCEEEECCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SYASNVLNKLKEQYPSQTLFHQAVAEVFESLEPALQKDKKYETYAILERLTIPDREIHF
CHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEE
RVSWTDDNGKIQTNRAYRIEFNAAIGPYKGGLRFHPSVNEGVIKFLGFEQILKNSLTTLA
EEEEECCCCEEEECCEEEEEEECCCCCCCCCEEECCCCCHHHHHHHHHHHHHHCCHHEEE
MGGGKGGSDFDPKGKSEGEIMRFCQAFMNELYRHIGATTDVPAGDIGVGGREIGYLFGQY
ECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHH
RKLTNCFEGVFTGKAIPWGGSLVRTEATGYGCVYFAQEMLKDRGESLEGKVCSVSGAGNV
HHHHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCCCE
AIYTIEKLLQLGAIPVTASDSQGMIYDKEGIDLALLKEVKEIKRLTLAEYAKARPQAVYT
EEHHHHHHHHHCCCEEECCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHH
KVADYPKDSNPVWAIPCFAAFPSATQNELNGNDAKTLLANGCKCVSEGANMPSTIEAVHQ
HHHCCCCCCCCEEEEEHHHHCCCCCCCCCCCCHHHHHHHCCCHHHHCCCCCCHHHHHHHH
FLDAKICYGPGKAANAGGVAVSGLEMAQNASMNPWTFEVVDKRLHTIMESIYANASETAK
HHCCEEEECCCCCCCCCCEEEEHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHH
EFGEPTNLVLGANIAGFRKVAGAMIDQGVI
HHCCCCCEEEECCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]