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The map label for this gene is rlmN

Identifier: 32266677

GI number: 32266677

Start: 1144064

End: 1145173

Strand: Direct

Name: rlmN

Synonym: HH1178

Alternate gene names: 32266677

Gene position: 1144064-1145173 (Clockwise)

Preceding gene: 32266676

Following gene: 32266678

Centisome position: 63.59

GC content: 36.13

Gene sequence:

>1110_bases
ATGAACATAGATTTTCCATTCCAACAACCAAGCCTTTATGGTTATAAGCTTAATGAGCTTAAATCCATTCTTTCTCCTGC
ATTTCGTGCGAAACAAATTTATCACTGGCTTTATCATCGCTACGAGAACGATGCAATGCGTATGGATAATATTTCAAAGA
CTATGCAAAATTACATACGTGAGCATTTTGCACTTTCTCAAATTTATCCTATACGGGTAGAACATAGCATTGATGGAAGT
AAAAAATATCTCTTTGAAACTTATGATGGACATTGTTTTGAATCTGTGTTGATTCAAATGCGTGATAAAAAATTGGGACA
CAAAGGCGAAGTGGTAGAGAGTGAAAAATGGACAATGTGTCTCTCAAGTCAAATTGGTTGTAAGGTGGGTTGTGCATTTT
GTTTTACTGCAAAGGGTGGATTTGTGCGGAATCTCCACGCAAGTGAAATAGTTGAGCAAGTTGTAATTATGAAAAAAGAC
AATCAAATGGCGCCCCATAAAAGAGTGAATATTGTTTATATGGGAATGGGTGAACCCCTTGATAATATTGAAAATGTAAC
ACGTGCAATCGAGATTCTCTCAGAATCCGAAGGATTAAGTATTTCTGCTCGGCGACAAACGATTTCTACGAGTGGTATCG
CACCTAAAATCAAACAATTAGGCAAGCTTAATTTAGGTGTGCAGCTTGCTATTTCGCTCCACGCAGTAGATGATAAGTTG
CGTTCTCAACTTATACCTATGAATAAAGCCTATAATATATCTGATATTCTCACTGAGGTAAGGGCATTTCCCATAGATAC
GCGCAAGAAAGTAATGTTTGAATATCTTATGATTAAAGGTGTGAATGATGACTTAAAAAGTGCAAAAAAATTGCTTCAAC
TCCTTAATGGTATTAAAGCAAAAGTAAATTTGATTTTGTTTAATCCACACGAAGGAAGCACATTTAAGCGACCGGAAATA
AACGATGTAAGAGCATTTGCCGATTTTTTAATAAAGCGGGGATTACTTTGCACTATACGAGAATCTAGAGGTATTGATAT
TAGTGCTGCGTGTGGGCAGTTACGCGAAAAAGTTAAAGCAGAAAATGTTTCTTGTGAAACATTGACATAA

Upstream 100 bases:

>100_bases
AGCGAAAACTCACTGAACAACTCCCTTTAATTCAAGATATTTCTTGTATCCTTAGCAAAGATTTGGTGCAGAATCATAAA
AGTTAAAATAAGAGATTGAT

Downstream 100 bases:

>100_bases
AAAATCTAAGAAAGGAGAAGCATATACAATGAATCTTGATATTATTTTAGCAGTATTTTTGGCAGCGGTTGTAGTCATAG
GTATCGTTTCTTTTTGGTAT

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MNIDFPFQQPSLYGYKLNELKSILSPAFRAKQIYHWLYHRYENDAMRMDNISKTMQNYIREHFALSQIYPIRVEHSIDGS
KKYLFETYDGHCFESVLIQMRDKKLGHKGEVVESEKWTMCLSSQIGCKVGCAFCFTAKGGFVRNLHASEIVEQVVIMKKD
NQMAPHKRVNIVYMGMGEPLDNIENVTRAIEILSESEGLSISARRQTISTSGIAPKIKQLGKLNLGVQLAISLHAVDDKL
RSQLIPMNKAYNISDILTEVRAFPIDTRKKVMFEYLMIKGVNDDLKSAKKLLQLLNGIKAKVNLILFNPHEGSTFKRPEI
NDVRAFADFLIKRGLLCTIRESRGIDISAACGQLREKVKAENVSCETLT

Sequences:

>Translated_369_residues
MNIDFPFQQPSLYGYKLNELKSILSPAFRAKQIYHWLYHRYENDAMRMDNISKTMQNYIREHFALSQIYPIRVEHSIDGS
KKYLFETYDGHCFESVLIQMRDKKLGHKGEVVESEKWTMCLSSQIGCKVGCAFCFTAKGGFVRNLHASEIVEQVVIMKKD
NQMAPHKRVNIVYMGMGEPLDNIENVTRAIEILSESEGLSISARRQTISTSGIAPKIKQLGKLNLGVQLAISLHAVDDKL
RSQLIPMNKAYNISDILTEVRAFPIDTRKKVMFEYLMIKGVNDDLKSAKKLLQLLNGIKAKVNLILFNPHEGSTFKRPEI
NDVRAFADFLIKRGLLCTIRESRGIDISAACGQLREKVKAENVSCETLT
>Mature_369_residues
MNIDFPFQQPSLYGYKLNELKSILSPAFRAKQIYHWLYHRYENDAMRMDNISKTMQNYIREHFALSQIYPIRVEHSIDGS
KKYLFETYDGHCFESVLIQMRDKKLGHKGEVVESEKWTMCLSSQIGCKVGCAFCFTAKGGFVRNLHASEIVEQVVIMKKD
NQMAPHKRVNIVYMGMGEPLDNIENVTRAIEILSESEGLSISARRQTISTSGIAPKIKQLGKLNLGVQLAISLHAVDDKL
RSQLIPMNKAYNISDILTEVRAFPIDTRKKVMFEYLMIKGVNDDLKSAKKLLQLLNGIKAKVNLILFNPHEGSTFKRPEI
NDVRAFADFLIKRGLLCTIRESRGIDISAACGQLREKVKAENVSCETLT

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=342, Percent_Identity=35.9649122807018, Blast_Score=215, Evalue=3e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_HELHP (Q7VGY9)

Other databases:

- EMBL:   AE017125
- RefSeq:   NP_860709.1
- ProteinModelPortal:   Q7VGY9
- GeneID:   1492866
- GenomeReviews:   AE017125_GR
- KEGG:   hhe:HH1178
- NMPDR:   fig|235279.1.peg.1178
- HOGENOM:   HBG335345
- OMA:   TFCSTAQ
- ProtClustDB:   PRK14462
- BioCyc:   HHEP235279:HH_1178-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 42015; Mature: 42015

Theoretical pI: Translated: 9.38; Mature: 9.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIDFPFQQPSLYGYKLNELKSILSPAFRAKQIYHWLYHRYENDAMRMDNISKTMQNYIR
CCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
EHFALSQIYPIRVEHSIDGSKKYLFETYDGHCFESVLIQMRDKKLGHKGEVVESEKWTMC
HHHHHHCEEEEEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCEECCCHHHEE
LSSQIGCKVGCAFCFTAKGGFVRNLHASEIVEQVVIMKKDNQMAPHKRVNIVYMGMGEPL
CCCCCCCCEEEEEEEECCCCCEECCCHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCH
DNIENVTRAIEILSESEGLSISARRQTISTSGIAPKIKQLGKLNLGVQLAISLHAVDDKL
HHHHHHHHHHHHHHCCCCCEEEHHHHHHCCCCCCHHHHHHCCCCCCEEEEEEEEHHHHHH
RSQLIPMNKAYNISDILTEVRAFPIDTRKKVMFEYLMIKGVNDDLKSAKKLLQLLNGIKA
HHHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCE
KVNLILFNPHEGSTFKRPEINDVRAFADFLIKRGLLCTIRESRGIDISAACGQLREKVKA
EEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHH
ENVSCETLT
CCCCCCCCC
>Mature Secondary Structure
MNIDFPFQQPSLYGYKLNELKSILSPAFRAKQIYHWLYHRYENDAMRMDNISKTMQNYIR
CCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
EHFALSQIYPIRVEHSIDGSKKYLFETYDGHCFESVLIQMRDKKLGHKGEVVESEKWTMC
HHHHHHCEEEEEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCEECCCHHHEE
LSSQIGCKVGCAFCFTAKGGFVRNLHASEIVEQVVIMKKDNQMAPHKRVNIVYMGMGEPL
CCCCCCCCEEEEEEEECCCCCEECCCHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCH
DNIENVTRAIEILSESEGLSISARRQTISTSGIAPKIKQLGKLNLGVQLAISLHAVDDKL
HHHHHHHHHHHHHHCCCCCEEEHHHHHHCCCCCCHHHHHHCCCCCCEEEEEEEEHHHHHH
RSQLIPMNKAYNISDILTEVRAFPIDTRKKVMFEYLMIKGVNDDLKSAKKLLQLLNGIKA
HHHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCE
KVNLILFNPHEGSTFKRPEINDVRAFADFLIKRGLLCTIRESRGIDISAACGQLREKVKA
EEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHH
ENVSCETLT
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12810954