Click here to switch to the map view.

The map label for this gene is hisF

Identifier: 32266675

GI number: 32266675

Start: 1142585

End: 1143340

Strand: Direct

Name: hisF

Synonym: HH1176

Alternate gene names: 32266675

Gene position: 1142585-1143340 (Clockwise)

Preceding gene: 32266674

Following gene: 32266676

Centisome position: 63.51

GC content: 39.02

Gene sequence:

>756_bases
ATGAGCCTTGCCAAAAGGATTATCCCGTGCCTTGATATTAATGGTGGGCGCGTAGTAAAAGGAGTGAATTTTATTGGATT
ACAAGATGCGGGCGACCCAGTAGAAATTGCTCGGCGCTATAATGATGAAGGTGCTGATGAAATAGTGCTTCTTGATATTA
CTGCAAGCTATGAGCAGCGCGGCATAATGATAGAGGTTGTAAAAAATATTGCAAAAGAGGTTTTTATTCCTTTTAGCGTA
GGAGGAGGGATAAGATCACTAGAGGATATGTCTGCATTATTAAATGCAGGTTGTGATAAAATTAGCATTAATAGTGCGGC
AATTAGAAATCCACAGCTTATTGATGAAAGTGCAAAGCGATTTGGTTCACAATGTATAGTGGTAGCGGTTGATGTGAAAA
AGGTATCCCAAAATTCTTGGAATGTTTATATTAATGGTGGGCGCGTAGATAGCGGTAAGGATATGTTAGAGTGGATAAAT
GAAGCGTGTGCAAGAGGTGCAGGAGAGATTCTCCTTACAAGTATAGATACTGATGGCACCAAAGGTGGTTATGACAAGGC
AATGATAGAAGCAGTTAAATCTATAAATGTGCCAATTATTGCAAGTGGAGGAGCTGGGAGTATGGAAGATTTTGCTGATG
TATTTGTTCACGGAGCTGATGCAGCTTTAGCTGCGAGTGTGTTTCATTATAAAGAAATTGAAATAAAAATGCTAAAGACT
TATTTGCAAGATAAAGGCGTGCCTGTAAGAATTTAA

Upstream 100 bases:

>100_bases
ATGATATATATAAAATCTACATATTTACACATAGATAAGATTCTTAGAGAAATCTCAAAATAAGAGTAAATTTATAGAAT
CAGGAATAAAAGGAGCAATA

Downstream 100 bases:

>100_bases
AAGAGGCAATAGAAAATTGGACGTAAGGATTAGATTTGGACAATAAATACAAAGTATTAATACCACAAAAGAAAGTTTTG
TTGCGAGGTAGAGAATGATA

Product: cyclase HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 251; Mature: 250

Protein sequence:

>251_residues
MSLAKRIIPCLDINGGRVVKGVNFIGLQDAGDPVEIARRYNDEGADEIVLLDITASYEQRGIMIEVVKNIAKEVFIPFSV
GGGIRSLEDMSALLNAGCDKISINSAAIRNPQLIDESAKRFGSQCIVVAVDVKKVSQNSWNVYINGGRVDSGKDMLEWIN
EACARGAGEILLTSIDTDGTKGGYDKAMIEAVKSINVPIIASGGAGSMEDFADVFVHGADAALAASVFHYKEIEIKMLKT
YLQDKGVPVRI

Sequences:

>Translated_251_residues
MSLAKRIIPCLDINGGRVVKGVNFIGLQDAGDPVEIARRYNDEGADEIVLLDITASYEQRGIMIEVVKNIAKEVFIPFSV
GGGIRSLEDMSALLNAGCDKISINSAAIRNPQLIDESAKRFGSQCIVVAVDVKKVSQNSWNVYINGGRVDSGKDMLEWIN
EACARGAGEILLTSIDTDGTKGGYDKAMIEAVKSINVPIIASGGAGSMEDFADVFVHGADAALAASVFHYKEIEIKMLKT
YLQDKGVPVRI
>Mature_250_residues
SLAKRIIPCLDINGGRVVKGVNFIGLQDAGDPVEIARRYNDEGADEIVLLDITASYEQRGIMIEVVKNIAKEVFIPFSVG
GGIRSLEDMSALLNAGCDKISINSAAIRNPQLIDESAKRFGSQCIVVAVDVKKVSQNSWNVYINGGRVDSGKDMLEWINE
ACARGAGEILLTSIDTDGTKGGYDKAMIEAVKSINVPIIASGGAGSMEDFADVFVHGADAALAASVFHYKEIEIKMLKTY
LQDKGVPVRI

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=257, Percent_Identity=47.0817120622568, Blast_Score=214, Evalue=4e-57,
Organism=Escherichia coli, GI87082028, Length=211, Percent_Identity=31.2796208530806, Blast_Score=100, Evalue=7e-23,
Organism=Saccharomyces cerevisiae, GI6319725, Length=316, Percent_Identity=34.1772151898734, Blast_Score=150, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_HELHP (Q7VGZ1)

Other databases:

- EMBL:   AE017125
- RefSeq:   NP_860707.1
- ProteinModelPortal:   Q7VGZ1
- SMR:   Q7VGZ1
- GeneID:   1492864
- GenomeReviews:   AE017125_GR
- KEGG:   hhe:HH1176
- NMPDR:   fig|235279.1.peg.1176
- HOGENOM:   HBG541613
- OMA:   RVVKGTN
- BioCyc:   HHEP235279:HH_1176-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 26953; Mature: 26822

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: ACT_SITE 12-12 ACT_SITE 131-131

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLAKRIIPCLDINGGRVVKGVNFIGLQDAGDPVEIARRYNDEGADEIVLLDITASYEQR
CCCHHHCCCEEECCCCEEEECEEEEEECCCCCHHHHHHHCCCCCCCEEEEEEEECCCCCC
GIMIEVVKNIAKEVFIPFSVGGGIRSLEDMSALLNAGCDKISINSAAIRNPQLIDESAKR
CCHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHCCCCEEEECHHHCCCCHHHHHHHHH
FGSQCIVVAVDVKKVSQNSWNVYINGGRVDSGKDMLEWINEACARGAGEILLTSIDTDGT
CCCEEEEEEEEEEECCCCCCEEEEECCEECCHHHHHHHHHHHHCCCCCCEEEEEECCCCC
KGGYDKAMIEAVKSINVPIIASGGAGSMEDFADVFVHGADAALAASVFHYKEIEIKMLKT
CCCHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
YLQDKGVPVRI
HHHCCCCEECC
>Mature Secondary Structure 
SLAKRIIPCLDINGGRVVKGVNFIGLQDAGDPVEIARRYNDEGADEIVLLDITASYEQR
CCHHHCCCEEECCCCEEEECEEEEEECCCCCHHHHHHHCCCCCCCEEEEEEEECCCCCC
GIMIEVVKNIAKEVFIPFSVGGGIRSLEDMSALLNAGCDKISINSAAIRNPQLIDESAKR
CCHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHCCCCEEEECHHHCCCCHHHHHHHHH
FGSQCIVVAVDVKKVSQNSWNVYINGGRVDSGKDMLEWINEACARGAGEILLTSIDTDGT
CCCEEEEEEEEEEECCCCCCEEEEECCEECCHHHHHHHHHHHHCCCCCCEEEEEECCCCC
KGGYDKAMIEAVKSINVPIIASGGAGSMEDFADVFVHGADAALAASVFHYKEIEIKMLKT
CCCHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
YLQDKGVPVRI
HHHCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12810954